FastQCFastQC Report
Wed 25 May 2016
SRR1780267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581025
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAT401.8058152E-533.0336413
GTTCTAA406.9930096E-427.52328539
GCATTTC406.9930096E-427.52328537
AAACTCG407.049611E-427.48535727
TCAGATG609.840916E-625.6331314
TGTACAG450.001389765524.46725512
CCATAGA450.001408023324.4125082
AGGGATA450.001408023324.4125088
CCGTATT602.8472464E-422.02622814
ATCGTAC500.002586824121.98828725
GCGTATC500.002595893621.97503944
TACCAGA654.971557E-420.281161
GTATCAA550.004456726420.01866334
CTTTAGA550.00449468319.98935325
ATGACTC550.004514884719.973877
ACTCGCA708.0329337E-418.8861421
ATCCGTA708.065626E-418.87473912
CGTCAGA708.188041E-418.8325062
ATTTAGT600.007430366718.32357226
CGAAACT600.007430366718.32357225
Produced by FastQC (version 0.11.5)