FastQCFastQC Report
Wed 25 May 2016
SRR1780267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581025
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT171552.952540768469515No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC47990.8259541327825825No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG45780.7879179037046599No Hit
CACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTG44770.7705348306871477TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC9950.17124908566757024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACT301.2849772E-436.7406161
TCTTCTG5950.035.8636544
CCGTCTT6400.033.3419941
GTCTTCT6450.033.08352343
CGTCTTC6600.032.66494442
GTACGTA353.216818E-431.42691213
ATGCCGT27500.030.63838244
TCTTTAT1700.029.8247321
TTCAGTC300.00574407129.33178517
CCAATTC300.00574407129.3317858
CAATTCG300.00574407129.3317859
AATTCGG300.00574407129.33178510
GGCGCTA454.0674146E-529.33178518
GCCGTCT7350.029.03247840
CTTTATA1750.028.9152492
GGCTAAA406.945415E-427.5554621
TATGCCG31150.026.9777143
CGCTATC508.337105E-526.39860520
ATCTATA609.745596E-625.66531223
TAGCCCG901.5099431E-824.4431535
Produced by FastQC (version 0.11.5)