FastQCFastQC Report
Wed 25 May 2016
SRR1780266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96920
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGTA200.006932937552.58
ATGTATG200.006932937552.525
TGCTCTT200.006932937552.546
GTATAGA200.006932937552.536
ACGCTTC501.698973E-435.00000415
TATAACC450.00428920631.1111093
TAAGAGA450.00428920631.1111094
CTTATAA604.951086E-429.1666661
TCTCGGT853.910027E-628.8235343
ATCTCGG1207.264134E-826.2542
AGATCTC1101.000908E-625.45454440
TAGATCT1251.0786425E-725.19999939
TTATACC901.9111989E-423.3333322
GTAGATC901.9111989E-423.33333235
AACCGTG1253.0438678E-622.470
TATACCC1003.8978958E-421.0000023
TGTAGAT1055.4171146E-420.034
TTGAACC2555.2750693E-1119.21568770
GATCTCG1107.409784E-419.09090841
GAACCGT1501.4735468E-518.66666669
Produced by FastQC (version 0.11.5)