##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96920 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.682996285596367 34.0 31.0 34.0 12.0 34.0 2 29.712360709863805 34.0 31.0 34.0 12.0 34.0 3 29.496450680974 34.0 31.0 34.0 11.0 34.0 4 29.4883718530747 34.0 30.0 34.0 11.0 34.0 5 29.392560874948412 34.0 27.0 34.0 11.0 34.0 6 32.24855550969872 37.0 31.0 38.0 11.0 38.0 7 32.537752785802724 37.0 31.0 38.0 12.0 38.0 8 32.432655798596784 37.0 31.0 38.0 11.0 38.0 9 32.78052001650846 38.0 33.0 38.0 12.0 38.0 10-11 32.49828724721419 38.0 31.5 38.0 11.0 38.0 12-13 32.57526826248453 38.0 31.5 38.0 11.0 38.0 14-15 32.465502476269094 38.0 31.0 38.0 11.0 38.0 16-17 32.48287247214197 38.0 31.0 38.0 11.0 38.0 18-19 32.5392282294676 38.0 31.0 38.0 11.0 38.0 20-21 32.531443458522496 38.0 31.0 38.0 11.0 38.0 22-23 32.52568097399917 38.0 31.5 38.0 11.0 38.0 24-25 32.5499845233182 38.0 31.5 38.0 11.0 38.0 26-27 32.48232562938506 38.0 31.0 38.0 11.0 38.0 28-29 32.555427156417664 38.0 32.0 38.0 11.0 38.0 30-31 32.55991023524557 38.0 32.0 38.0 11.0 38.0 32-33 32.60699546017334 38.0 32.5 38.0 11.0 38.0 34-35 32.57209038382171 38.0 32.5 38.0 11.0 38.0 36-37 32.51244841106067 38.0 31.5 38.0 11.0 38.0 38-39 32.50543747420553 38.0 31.5 38.0 11.0 38.0 40-41 32.56435720181593 38.0 32.5 38.0 11.0 38.0 42-43 32.650980189847296 38.0 33.0 38.0 11.0 38.0 44-45 32.75375567478333 38.0 34.0 38.0 11.0 38.0 46-47 32.64969562525795 38.0 33.5 38.0 11.0 38.0 48-49 32.592137845645894 38.0 33.0 38.0 11.0 38.0 50-51 32.53289826661164 38.0 32.5 38.0 11.0 38.0 52-53 32.58914052827074 38.0 32.0 38.0 11.0 38.0 54-55 32.59238547255468 38.0 32.0 38.0 11.0 38.0 56-57 32.553549319026004 38.0 32.0 38.0 11.0 38.0 58-59 32.58808295501444 38.0 32.0 38.0 11.0 38.0 60-61 32.608403838217086 38.0 32.0 38.0 11.0 38.0 62-63 32.46781881964507 38.0 31.0 38.0 11.0 38.0 64-65 32.40928600907965 38.0 31.0 38.0 11.0 38.0 66-67 32.33028270738754 37.5 31.0 38.0 11.0 38.0 68-69 32.246796326867525 37.0 31.0 38.0 11.0 38.0 70-71 32.2320831613702 37.0 31.0 38.0 11.0 38.0 72-73 32.14352558811391 37.5 31.0 38.0 11.0 38.0 74-75 32.10851733388362 38.0 30.5 38.0 11.0 38.0 76 30.601619892695005 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37988396779619293 1101 2 0.7143131768626034 1101 3 0.1742976947890753 1101 4 0.15972820888650574 1101 5 -0.5674001903121102 1101 6 -0.17150740739593573 1101 7 -0.14002198041294633 1101 8 -0.329434868533788 1101 9 0.17122292483165324 1101 10-11 -0.08769172667047087 1101 12-13 0.14289782158282094 1101 14-15 -0.21227746967086603 1101 16-17 -0.7004306589323583 1101 18-19 0.02359414637105317 1101 20-21 0.16817956738218243 1101 22-23 0.3534588954771465 1101 24-25 -0.29404590340286063 1101 26-27 -0.5173612253671465 1101 28-29 -0.3574504958850824 1101 30-31 0.23335690507873608 1101 32-33 -0.44476341819246556 1101 34-35 -0.2545441649305715 1101 36-37 0.46888127315191497 1101 38-39 0.04115034119766392 1101 40-41 0.2123147404439294 1101 42-43 -0.2694229779563386 1101 44-45 0.4519295997942834 1101 46-47 0.3790138456800989 1101 48-49 0.09375983894656059 1101 50-51 0.10207711434561872 1101 52-53 0.09530535683480679 1101 54-55 0.1855686213176142 1101 56-57 -0.11249480466691608 1101 58-59 -0.017927964698614574 1101 60-61 0.1270335914244285 1101 62-63 0.012507938523491191 1101 64-65 -0.26922082790291313 1101 66-67 -0.8084851660029528 1101 68-69 -0.4202437836823698 1101 70-71 0.14900841643294171 1101 72-73 0.06726191494491474 1101 74-75 -0.24371842874170113 1101 76 -0.2296881323295601 1102 1 0.008666163345083078 1102 2 0.07215263672756933 1102 3 -0.007904105661037164 1102 4 -0.18651460181617097 1102 5 -0.027358929997031822 1102 6 -0.18035336888630837 1102 7 -0.5297444110205944 1102 8 -0.0313290920896776 1102 9 -0.26504239149742403 1102 10-11 0.17118549263435057 1102 12-13 0.35343483083514116 1102 14-15 0.24804073488027 1102 16-17 0.3676957476692948 1102 18-19 0.18669742219000796 1102 20-21 0.22191175042794242 1102 22-23 -0.32105348261737277 1102 24-25 -0.19697476061716657 1102 26-27 0.03746498118449182 1102 28-29 0.23368166215442443 1102 30-31 0.3105480778719283 1102 32-33 0.6769857691043626 1102 34-35 0.2493786907833595 1102 36-37 0.23980837992861126 1102 38-39 -0.0047517593274619685 1102 40-41 0.28244164717060727 1102 42-43 0.24812828485936222 1102 44-45 0.16474842950171364 1102 46-47 -0.36090550915861286 1102 48-49 0.18015643809633985 1102 50-51 0.0990919930653007 1102 52-53 0.11657629957048954 1102 54-55 0.01663576310305359 1102 56-57 -0.010141091238558886 1102 58-59 -0.22204149308070953 1102 60-61 0.09541631210459656 1102 62-63 0.15599693577279794 1102 64-65 0.6770938757729965 1102 66-67 0.4047712731068245 1102 68-69 0.2200412875854454 1102 70-71 0.27042939667799715 1102 72-73 -0.10426534186928649 1102 74-75 -0.033603399984514226 1102 76 -0.15635729962138356 1103 1 1.2867558006969482 1103 2 0.5032762222216434 1103 3 0.7382422010305767 1103 4 1.143521930305365 1103 5 0.45838463233033266 1103 6 0.13743153711030232 1103 7 0.9419209191050371 1103 8 0.5693569989733192 1103 9 0.399485809518346 1103 10-11 0.8593481942292911 1103 12-13 1.372836951480238 1103 14-15 0.14630768667569072 1103 16-17 0.6968858038040153 1103 18-19 0.7213138349138788 1103 20-21 0.6651978593114904 1103 22-23 0.8230701919794399 1103 24-25 0.795830193494929 1103 26-27 0.44179091899078315 1103 28-29 1.099314737089827 1103 30-31 0.42872058076777364 1103 32-33 1.128357951230349 1103 34-35 1.0173551676611936 1103 36-37 1.110141624159212 1103 38-39 0.3766079484155611 1103 40-41 0.6608609646436534 1103 42-43 0.7567712501984047 1103 44-45 0.5244561725294261 1103 46-47 0.2511634563586256 1103 48-49 0.5398419054401913 1103 50-51 0.08425638761221421 1103 52-53 0.7755133039365205 1103 54-55 0.16445720122121088 1103 56-57 0.7349359826276114 1103 58-59 0.5329928114724396 1103 60-61 0.6833384363138109 1103 62-63 0.347857400889076 1103 64-65 1.101445653372572 1103 66-67 0.7031095070906019 1103 68-69 0.5192259975560418 1103 70-71 0.37917928529957834 1103 72-73 0.3707858922035996 1103 74-75 0.6990883936535468 1103 76 0.34270712165634976 1104 1 0.41112603028031813 1104 2 0.3449964382291242 1104 3 0.6325463612864084 1104 4 0.6487109368519626 1104 5 0.8176328297539293 1104 6 0.45774710111307826 1104 7 0.5169503335316818 1104 8 -0.4461798520237572 1104 9 -0.1348159372460671 1104 10-11 0.003938956590886278 1104 12-13 0.08735108548934534 1104 14-15 0.05212118412644173 1104 16-17 -0.3116670064050524 1104 18-19 0.6989814971457591 1104 20-21 0.3580131970494449 1104 22-23 0.25773245402426426 1104 24-25 0.23676753322401822 1104 26-27 -0.38788446641363095 1104 28-29 -0.4168707397255744 1104 30-31 -0.5475517817386404 1104 32-33 -0.15993359853283096 1104 34-35 -0.17026362136948592 1104 36-37 -0.21639622240307688 1104 38-39 0.1190808720587313 1104 40-41 -0.46502523923603434 1104 42-43 -0.15085236581511907 1104 44-45 -0.429785538635997 1104 46-47 -0.3274409422294795 1104 48-49 -0.25555435968183815 1104 50-51 -0.10171066822994135 1104 52-53 -0.10314841507274508 1104 54-55 0.08182528883094164 1104 56-57 0.16720737973745514 1104 58-59 -0.1718522725472269 1104 60-61 -0.1871174118775798 1104 62-63 0.0637366659809473 1104 64-65 0.1468796292979988 1104 66-67 0.3628223016119847 1104 68-69 -0.5993141418230863 1104 70-71 0.03875632092874781 1104 72-73 0.5552445159101254 1104 74-75 -0.006608954522754118 1104 76 -0.08814634924599218 1105 1 -0.13213614749878744 1105 2 0.0944799866258812 1105 3 -0.2668129393333345 1105 4 -0.16154923499918183 1105 5 0.10558828018954713 1105 6 0.093042451551689 1105 7 -0.011970956250060283 1105 8 -0.37746933437458097 1105 9 0.22318232627897316 1105 10-11 -0.03280395529797886 1105 12-13 -1.1233461990230893 1105 14-15 -0.3885392751412482 1105 16-17 -0.4756591024500807 1105 18-19 -0.2801284097432628 1105 20-21 -0.018914140455422057 1105 22-23 -0.17709734149083545 1105 24-25 -0.10367886059653131 1105 26-27 -0.0022747541295657925 1105 28-29 -0.16726743443530978 1105 30-31 0.1549271859569359 1105 32-33 -0.5194138536150064 1105 34-35 -0.14706072325266462 1105 36-37 0.2553785747586872 1105 38-39 0.07151493091924266 1105 40-41 -0.4497038416730845 1105 42-43 -0.1342810056761934 1105 44-45 -0.2510708294955464 1105 46-47 -0.16033516695328842 1105 48-49 -0.3561052413919654 1105 50-51 -0.2245587865497498 1105 52-53 -0.9057142065161123 1105 54-55 -0.046853493241442834 1105 56-57 -0.4305142453635895 1105 58-59 -0.19375281444717274 1105 60-61 -0.5943956370804173 1105 62-63 -0.5120919020260004 1105 64-65 -0.6457463399843597 1105 66-67 -0.007989949518218964 1105 68-69 -0.06837943926927181 1105 70-71 -0.48506033126729875 1105 72-73 -0.06631621580449831 1105 74-75 -0.30906247387846264 1105 76 -0.5324294545422426 1106 1 0.8216953821882527 1106 2 0.9311866259941155 1106 3 0.6465681662530933 1106 4 1.0920483081420898 1106 5 0.2964335565861518 1106 6 0.5348821707678297 1106 7 0.19360084837117597 1106 8 0.39246339449350387 1106 9 0.7194239173874877 1106 10-11 0.40287899293250007 1106 12-13 0.9132141987540336 1106 14-15 0.27354759236387594 1106 16-17 0.6860767852363736 1106 18-19 0.3105048163462385 1106 20-21 0.49485450534744047 1106 22-23 0.6471933867922885 1106 24-25 0.7123126573911875 1106 26-27 0.4971549036651126 1106 28-29 0.48606687384196334 1106 30-31 0.7325814708355551 1106 32-33 1.0809809986809853 1106 34-35 0.6120501278733954 1106 36-37 0.606877551067555 1106 38-39 0.8568761476492739 1106 40-41 1.1158197032230817 1106 42-43 1.10839960268882 1106 44-45 0.6061169447074377 1106 46-47 0.7833252600721607 1106 48-49 0.6997151739341518 1106 50-51 0.6115994468518267 1106 52-53 0.6479418332616049 1106 54-55 0.45402395630520687 1106 56-57 0.5955386940923901 1106 58-59 0.7755583876587053 1106 60-61 0.7920770157420449 1106 62-63 0.8876009906326701 1106 64-65 0.8630062192434877 1106 66-67 0.9916078861062232 1106 68-69 0.9991110550273063 1106 70-71 0.8701312452170598 1106 72-73 0.6860894583692314 1106 74-75 0.9003262362707076 1106 76 1.3314781207721786 1107 1 -0.21676615627328388 1107 2 -1.0649672201597298 1107 3 0.18502970471463343 1107 4 -1.1310286149348343 1107 5 -0.8035664434138461 1107 6 -0.9843485984629368 1107 7 -1.241014536244208 1107 8 -0.06907506704495603 1107 9 -0.01903762107404816 1107 10-11 0.04903283908635103 1107 12-13 0.04013727567711811 1107 14-15 -0.143760099943826 1107 16-17 0.06299986215945097 1107 18-19 0.14127404711546632 1107 20-21 -0.2474531869602501 1107 22-23 -0.07203738243848079 1107 24-25 -0.2607642656857436 1107 26-27 -0.5778450963348902 1107 28-29 -0.04470235692727442 1107 30-31 -0.6039569907029048 1107 32-33 -0.6036343859343987 1107 34-35 -0.35718064135737393 1107 36-37 -0.6546609104709127 1107 38-39 -0.5700469292738006 1107 40-41 -0.757257219853841 1107 42-43 -0.4108311665419464 1107 44-45 -0.03426767067718117 1107 46-47 -0.42821320146630626 1107 48-49 -0.06374636452738969 1107 50-51 -0.07493901468663466 1107 52-53 -0.275135089815322 1107 54-55 -0.5978991206178748 1107 56-57 -0.7775382289845325 1107 58-59 -0.6552108431105239 1107 60-61 -0.696384522719498 1107 62-63 -0.5104759324442512 1107 64-65 -0.7754553192180538 1107 66-67 -0.6853151908168513 1107 68-69 -0.5605043295880776 1107 70-71 -0.8798687547829438 1107 72-73 -0.517756695476919 1107 74-75 -0.8169814560369915 1107 76 -0.09929110999705415 1108 1 -0.16918289662105934 1108 2 -0.10591918007720835 1108 3 0.5964797489232971 1108 4 0.6875832329873575 1108 5 0.29746509210044536 1108 6 0.4592706175700698 1108 7 0.30590559343453805 1108 8 0.28200067713423493 1108 9 0.7443133957252428 1108 10-11 0.7712255888653061 1108 12-13 0.26172584036912383 1108 14-15 0.9968823993193681 1108 16-17 0.2390313976737488 1108 18-19 0.9531146011974059 1108 20-21 0.49469977502030105 1108 22-23 0.7617675099871022 1108 24-25 0.37054283717308323 1108 26-27 0.8211012638183703 1108 28-29 0.04184347272200739 1108 30-31 0.5379159830666183 1108 32-33 0.015331131306979273 1108 34-35 0.4675467385424241 1108 36-37 0.18102285021284814 1108 38-39 0.4379106304078988 1108 40-41 0.3313590489348428 1108 42-43 0.5786103306581722 1108 44-45 0.5225404637171636 1108 46-47 0.41591441421303443 1108 48-49 0.6298802196164388 1108 50-51 0.44249393550493465 1108 52-53 0.1548236487641006 1108 54-55 0.4364878525622018 1108 56-57 0.5050509251563469 1108 58-59 0.6479353402080719 1108 60-61 0.6833384363138109 1108 62-63 0.7731447572109218 1108 64-65 0.8236678755947935 1108 66-67 1.155216786784095 1108 68-69 0.8391493692035468 1108 70-71 1.0017846492842537 1108 72-73 0.12940658185877396 1108 74-75 0.37150218675699875 1108 76 0.6070749377483047 1109 1 0.45736657374179757 1109 2 0.07794379039833998 1109 3 0.5499618314114656 1109 4 0.21685977118884736 1109 5 0.37742903169927544 1109 6 1.0904628645838201 1109 7 0.9914138197135571 1109 8 0.10679220293996394 1109 9 0.560675802757018 1109 10-11 0.6021248451195333 1109 12-13 0.3335761897042602 1109 14-15 0.6364812273563274 1109 16-17 1.1091914157039469 1109 18-19 0.67283513308832 1109 20-21 0.8995679291785166 1109 22-23 0.6196669916188284 1109 24-25 0.24176212948771791 1109 26-27 0.7314686291553087 1109 28-29 0.3417983972205434 1109 30-31 0.5523401435354032 1109 32-33 0.5875420846538333 1109 34-35 0.637841983077017 1109 36-37 0.6019378828925852 1109 38-39 0.8617781084335974 1109 40-41 0.5049222673256537 1109 42-43 0.6333996026888187 1109 44-45 0.34755736703021256 1109 46-47 0.5930160594688374 1109 48-49 1.119850920540486 1109 50-51 1.1240805931564992 1109 52-53 1.204879993140942 1109 54-55 0.7457283303625175 1109 56-57 0.8429368841376359 1109 58-59 0.8933939834354803 1109 60-61 1.1583666085022273 1109 62-63 0.9615828910851647 1109 64-65 0.6349142132103012 1109 66-67 0.6503937081273392 1109 68-69 0.4942090942429971 1109 70-71 0.47088539303003074 1109 72-73 0.2663684930600354 1109 74-75 0.3027395092722074 1109 76 1.1995022535021924 1110 1 0.22214965232756256 1110 2 0.7820043122448723 1110 3 0.5380581723100306 1110 4 0.959734558899207 1110 5 0.5499162943208589 1110 6 0.9774321404831596 1110 7 0.11537553038208159 1110 8 0.19161542356679462 1110 9 0.597467527320859 1110 10-11 1.0275610038349896 1110 12-13 0.6798707705287228 1110 14-15 0.6288928376697456 1110 16-17 0.5367581904453402 1110 18-19 0.3918948829725295 1110 20-21 0.2851785513801488 1110 22-23 0.8167875721345013 1110 24-25 0.6422793442476333 1110 26-27 0.94872970681471 1110 28-29 1.1047828035815144 1110 30-31 0.8737080607810412 1110 32-33 0.6333734880813466 1110 34-35 0.41635055185885506 1110 36-37 0.6576195256284194 1110 38-39 0.7194745728461314 1110 40-41 1.2259196426537358 1110 42-43 0.44560432709827325 1110 44-45 0.33793099679708405 1110 46-47 0.29263779792800193 1110 48-49 0.5554692623653992 1110 50-51 0.5951397254708439 1110 52-53 0.9125996164233214 1110 54-55 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2116 24-25 0.31852911535182926 2116 26-27 0.5917285470759666 2116 28-29 -0.0725198882331739 2116 30-31 -0.40456820119784 2116 32-33 -0.21448709911270214 2116 34-35 -0.16341200689106472 2116 36-37 -0.306703247978362 2116 38-39 0.12315521741672342 2116 40-41 -0.7369381977906357 2116 42-43 -0.13946861436544822 2116 44-45 -0.04842986507193103 2116 46-47 -0.374471400631478 2116 48-49 -0.13185906577962925 2116 50-51 -0.214861495306792 2116 52-53 -0.28071052213493886 2116 54-55 -0.2508060973620587 2116 56-57 -0.5857224865873931 2116 58-59 -0.1464600977318753 2116 60-61 0.05084266869373977 2116 62-63 -0.4389643045372722 2116 64-65 -0.30546426376188407 2116 66-67 0.11213561419209839 2116 68-69 0.7471730705311828 2116 70-71 0.5572511021043525 2116 72-73 -0.4065909232646341 2116 74-75 -0.21577394262017435 2116 76 0.21961169262667823 2117 1 -0.47100477479761693 2117 2 -0.37791855453963663 2117 3 -0.20201896090546256 2117 4 -0.371295490915994 2117 5 -0.8955601639789954 2117 6 -0.9997332138475521 2117 7 -0.5438402819270962 2117 8 -1.1281017546430725 2117 9 -1.0415650936015233 2117 10-11 -1.2729451828916751 2117 12-13 -0.9721861152177027 2117 14-15 -0.8421902412311049 2117 16-17 -1.0213407971812067 2117 18-19 -0.5173209293366483 2117 20-21 -0.6128770488126776 2117 22-23 -0.21426658181053426 2117 24-25 -0.7834879705523008 2117 26-27 -0.5324369330695831 2117 28-29 -1.0807306143840982 2117 30-31 -0.36082512257103616 2117 32-33 -0.9026139777711357 2117 34-35 -0.676803875266323 2117 36-37 -1.1452810046624364 2117 38-39 -1.1380218115343936 2117 40-41 -0.8773906578444226 2117 42-43 -0.5053759075152584 2117 44-45 -0.609385410080634 2117 46-47 -1.1007014275259621 2117 48-49 -0.9619017805399466 2117 50-51 -0.0943267697886796 2117 52-53 -1.0028430960947574 2117 54-55 -1.1371848349035858 2117 56-57 -1.1074832839295858 2117 58-59 -0.46262842552810923 2117 60-61 -0.6193044599251465 2117 62-63 -1.2385371569340506 2117 64-65 -0.3369545342886937 2117 66-67 -1.2942241390115115 2117 68-69 -0.9304807817073879 2117 70-71 -0.43159559936692915 2117 72-73 -1.4448760047390863 2117 74-75 -1.0431588500715279 2117 76 -1.366324077030022 2118 1 -1.1495353870425156 2118 2 -0.6654287586212675 2118 3 -0.5465087568238296 2118 4 -1.0053363072425263 2118 5 -0.8983356741830768 2118 6 -0.9997332138475521 2118 7 -0.4468606900903609 2118 8 -0.32630583627572207 2118 9 -0.9924222364586655 2118 10-11 -1.912659468605959 2118 12-13 -1.3672473397074967 2118 14-15 -0.9280677922515146 2118 16-17 -1.3407693686097772 2118 18-19 -1.1743413374999143 2118 20-21 -1.0735301100371686 2118 22-23 -0.43896045936155303 2118 24-25 -0.6976104195318911 2118 26-27 -1.0668450963348874 2118 28-29 -0.8862408184657298 2118 30-31 -1.2331108368567527 2118 32-33 -0.42363438593440605 2118 34-35 -1.1553344875112188 2118 36-37 -0.9911993720093726 2118 38-39 -0.7111238523507204 2118 40-41 -1.0224110660076846 2118 42-43 -0.654600397311178 2118 44-45 -0.697344593754103 2118 46-47 -0.7469055091586121 2118 48-49 -1.4252079029889266 2118 50-51 -0.8209390146866369 2118 52-53 -0.5319043205845517 2118 54-55 -0.826899120617874 2118 56-57 -1.315768998215301 2118 58-59 -0.7520569969566786 2118 60-61 -1.4686922150271862 2118 62-63 -0.8294759324442538 2118 64-65 -1.2523014730642075 2118 66-67 -1.4044690369706956 2118 68-69 -0.9528889449726918 2118 70-71 -0.8423302932444798 2118 72-73 -0.894141310861535 2118 74-75 -0.8167506868062233 2118 76 -0.6537526484585925 2119 1 -0.1524972191035836 2119 2 -0.5816730334304303 2119 3 -1.747119443846728 2119 4 -0.5534889789982529 2119 5 -0.03357231540445227 2119 6 -0.22645077109946143 2119 7 -0.300204201540744 2119 8 -0.6978478210085477 2119 9 -0.25007109142050155 2119 10-11 -0.12469000295710941 2119 12-13 0.14956182059784595 2119 14-15 -0.1307242808011324 2119 16-17 0.09692528787876853 2119 18-19 -0.2565092764312098 2119 20-21 -0.432934690189839 2119 22-23 -0.8668993906592668 2119 24-25 -0.10029744243265526 2119 26-27 -0.42994433297611323 2119 28-29 -0.20624081846573006 2119 30-31 -0.33465282158957166 2119 32-33 -0.3570695004382145 2119 34-35 -0.5441741821677084 2119 36-37 0.1420067348608569 2119 38-39 -0.7679330126560657 2119 40-41 -0.14176984463364306 2119 42-43 -0.13988284005926488 2119 44-45 -0.46306978459379877 2119 46-47 -0.6242642877845661 2119 48-49 -0.0023987426835887504 2119 50-51 -0.551656571938544 2119 52-53 0.09295064124750496 2119 54-55 -0.33282278474001004 2119 56-57 -0.36718884554354503 2119 58-59 -0.24207226413224703 2119 60-61 -0.19937923792794976 2119 62-63 0.041241624807661026 2119 64-65 -0.43101903031611855 2119 66-67 -0.47822476216153476 2119 68-69 -1.2737897083314706 2119 70-71 -0.7129867817941005 2119 72-73 -0.06997337193023867 2119 74-75 -0.44336137382912 2119 76 -0.2606534118173691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 12.0 13 238.0 14 992.0 15 1780.0 16 2484.0 17 2855.0 18 2918.0 19 2568.0 20 1881.0 21 1368.0 22 1040.0 23 866.0 24 778.0 25 843.0 26 826.0 27 979.0 28 1124.0 29 1399.0 30 1574.0 31 1951.0 32 2444.0 33 3284.0 34 4358.0 35 6594.0 36 12081.0 37 39682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.07697069748246 22.81778786628147 10.988444077589765 29.116797358646306 2 19.797771357820885 22.72595955427156 29.91023524556335 27.5660338423442 3 19.450061906727196 20.426124638877425 28.386297977713575 31.7375154766818 4 15.93582335947173 18.143829962855964 26.91085431283533 39.00949236483698 5 17.023318200577798 30.041271151465125 31.20408584399505 21.73132480396203 6 30.763516302104826 31.740610813041688 15.992571192736277 21.503301692117212 7 28.305819232356583 24.289104416013206 18.068510111432108 29.336566240198103 8 24.79777135782088 28.416219562525797 20.69541890218737 26.09059017746595 9 23.467808501857203 16.734420140321916 18.9991745769707 40.79859678085019 10-11 27.54488237721833 21.44913330581923 23.208316137020223 27.79766817994222 12-13 25.476681799422202 19.192633099463475 23.106685926537352 32.223999174576974 14-15 25.151155592241025 20.245047461824186 21.166941807676434 33.43685513825836 16-17 26.667354519191083 21.39341725134131 21.332542302930253 30.606685926537352 18-19 25.778992983904253 20.793437886917047 22.195109368551382 31.232459760627325 20-21 25.582955014444902 21.364527445315723 22.507222451506397 30.545295088732978 22-23 27.308089145687163 20.861535286834503 21.421791167973588 30.408584399504747 24-25 26.194799834915393 21.792199752373094 20.792406108130418 31.220594304581095 26-27 25.357511349566654 22.38495666529096 22.390115559224103 29.86741642591828 28-29 25.968324391250512 21.416116384647133 23.545707800247627 29.069851423854725 30-31 25.973999174576974 20.894036318613292 23.37546430045398 29.756500206355756 32-33 24.712133718530747 22.268365662401983 23.417251341312422 29.60224927775485 34-35 25.052620718118035 21.62556747833265 23.550350804787453 29.771460998761867 36-37 25.326557985967806 20.120718118035494 24.296326867519603 30.256397028477096 38-39 23.99607924061081 20.34203466776723 26.331510524143624 29.330375567478335 40-41 23.967189434585226 19.829756500206354 26.584296326867516 29.6187577383409 42-43 22.448411060668594 20.222864217911678 26.53528683450268 30.793437886917047 44-45 21.6678704085844 20.25846058605035 28.208316137020223 29.865352868345024 46-47 21.693148988856787 21.254127115146513 28.04323153115972 29.00949236483698 48-49 21.65652084193149 21.660647957078 27.47833264548081 29.2044985555097 50-51 21.19325216673545 22.833780437474203 26.17725959554272 29.795707800247627 52-53 20.23122281893737 22.87929673595097 26.54677335314359 30.342707091968073 54-55 20.58140734626496 22.22915806851011 26.33512174989682 30.854312835328106 56-57 20.672826417800337 22.523847110230655 25.99786420689335 30.805462265075654 58-59 21.231021621036014 22.446463302018685 25.293671552164426 31.028843524780875 60-61 21.268572018159308 21.286112257531986 25.883718530747007 31.561597193561703 62-63 20.95697482459761 21.836050350804786 25.603590590177465 31.60338423442014 64-65 21.62608336772596 22.25185720181593 24.64764754436649 31.474411886091623 66-67 21.531159719356168 23.31304168386298 23.14073462649608 32.01506397028477 68-69 21.13661337501741 24.349073199923648 23.41221322850407 31.102100196554872 70-71 21.196347503095335 24.777651671481635 24.05282707387536 29.973173751547666 72-73 21.425402393726785 23.912505158893932 25.2455633512175 29.41652909616178 74-75 20.54839042509286 25.67375154766818 24.880829550144448 28.897028477094512 76 20.290961617829137 25.30747007841519 23.42963268675196 30.971935617003716 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.0 8 0.0 9 0.5 10 0.5 11 1.0 12 2.0 13 1.0 14 1.5 15 4.0 16 4.5 17 4.0 18 8.0 19 13.5 20 27.5 21 40.0 22 47.0 23 64.5 24 94.0 25 113.0 26 161.5 27 254.5 28 378.0 29 457.0 30 549.5 31 718.5 32 936.5 33 1078.0 34 1191.0 35 1519.5 36 1834.0 37 1933.0 38 2115.0 39 2598.5 40 3148.0 41 3491.0 42 3586.0 43 3733.5 44 4036.5 45 4136.5 46 4081.0 47 3999.0 48 3838.0 49 3516.0 50 3273.0 51 3114.0 52 2740.0 53 2478.0 54 2431.0 55 2425.0 56 2424.5 57 2519.0 58 2608.0 59 2675.0 60 2888.0 61 3024.5 62 3015.0 63 3113.5 64 3135.5 65 3129.5 66 3226.5 67 3253.0 68 3210.0 69 3082.0 70 2877.0 71 2757.0 72 2554.0 73 2055.0 74 1563.0 75 1367.0 76 1098.0 77 619.5 78 300.0 79 190.0 80 122.0 81 35.0 82 14.5 83 13.0 84 10.0 85 8.0 86 6.0 87 3.0 88 4.5 89 6.5 90 5.5 91 4.0 92 4.0 93 3.5 94 2.5 95 2.5 96 3.0 97 3.0 98 2.5 99 7.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.158893933140734E-4 54-55 0.0 56-57 5.158893933140734E-4 58-59 5.158893933140734E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.158893933140734E-4 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 96920.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.64857614527446 #Duplication Level Percentage of deduplicated Percentage of total 1 78.88966212665635 57.31221626083368 2 13.28627629205664 19.30458109781263 3 4.308985811875985 9.391250515889393 4 1.6204854355143374 4.709038382170863 5 0.7626649245146355 2.7703260420965745 6 0.4047663007200579 1.7643417251341313 7 0.24996094360256207 1.2711514651258768 8 0.16474698555623413 0.9574907139909203 9 0.10935791282612092 0.7150226991333059 >10 0.2030932666770817 1.804581097812629 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0020635575732562937 0.0 2 0.0 0.0 0.0 0.005158893933140734 0.0 3 0.0 0.0 0.0 0.006190672719768881 0.0 4 0.0 0.0 0.0 0.0175402393726785 0.0 5 0.0 0.0 0.0 0.021667354519191086 0.0 6 0.0 0.0 0.0 0.021667354519191086 0.0 7 0.0 0.0 0.0 0.02373091209244738 0.0 8 0.0 0.0 0.0 0.024762690879075525 0.0 9 0.0 0.0 0.0 0.036112257531985145 0.0 10 0.0 0.0 0.0 0.04023937267849773 0.0 11 0.0 0.0 0.0 0.04230293025175402 0.0 12 0.0 0.0 0.0 0.04333470903838217 0.0 13 0.0 0.0 0.0 0.04849360297152291 0.0 14 0.0 0.0 0.0 0.0505571605447792 0.0 15 0.0 0.0 0.0 0.051588939331407346 0.0 16 0.0 0.0 0.0 0.053652496904663644 0.0 17 0.0 0.0 0.0 0.055716054477919934 0.0 18 0.0 0.0 0.0 0.05984316962443252 0.0 19 0.0 0.0 0.0 0.0660338423442014 0.0 20 0.0 0.0 0.0 0.07635163021048287 0.0 21 0.0 0.0 0.0 0.0835740817168799 0.0 22 0.0 0.0 0.0 0.09182831200990507 0.0 23 0.0 0.0 0.0 0.09698720594304581 0.0 24 0.0 0.0 0.0 0.10317787866281469 0.0 25 0.0 0.0010317787866281469 0.0 0.11555922410235246 0.0 26 0.0 0.0010317787866281469 0.0 0.14135369376805612 0.0 27 0.0 0.0010317787866281469 0.0 0.24246801485761452 0.0 28 0.0 0.0010317787866281469 0.0 0.3631861328931077 0.0 29 0.0 0.0010317787866281469 0.0 0.5169211721007017 0.0 30 0.0 0.0010317787866281469 0.0 0.6912917870408585 0.0 31 0.0 0.0010317787866281469 0.0 0.8739166322740405 0.0 32 0.0 0.0010317787866281469 0.0 1.0751134956665291 0.0 33 0.0 0.0010317787866281469 0.0 1.405282707387536 0.0 34 0.0 0.0010317787866281469 0.0 1.7787866281469253 0.0 35 0.0 0.0010317787866281469 0.0 2.338010730499381 0.0 36 0.0 0.0010317787866281469 0.0 3.119067271976888 0.0 37 0.0 0.0010317787866281469 0.0 4.083780437474205 0.0 38 0.0 0.0010317787866281469 0.0 5.038175815105242 0.0 39 0.0 0.0010317787866281469 0.0 6.011143210895584 0.0 40 0.0 0.0010317787866281469 0.0 6.862360709863805 0.0 41 0.0 0.0010317787866281469 0.0 7.806438299628559 0.0 42 0.0 0.0010317787866281469 0.0 8.693768056128766 0.0 43 0.0 0.0010317787866281469 0.0 9.651258770119686 0.0 44 0.0 0.0010317787866281469 0.0 10.60668592653735 0.0 45 0.0 0.0010317787866281469 0.0 11.59203466776723 0.0 46 0.0 0.0010317787866281469 0.0 12.491745769706975 0.0 47 0.0 0.0010317787866281469 0.0 13.425505571605449 0.0 48 0.0 0.0010317787866281469 0.0 14.289104416013206 0.0 49 0.0 0.0010317787866281469 0.0 14.963887742468016 0.0 50 0.0 0.0010317787866281469 0.0 15.733594717292613 0.0 51 0.0 0.0010317787866281469 0.0 16.45996698307883 0.0 52 0.0 0.0010317787866281469 0.0 17.236896409409823 0.0 53 0.0 0.0010317787866281469 0.0 17.985967808501858 0.0 54 0.0 0.0010317787866281469 0.0 18.744325216673545 0.0 55 0.0 0.0010317787866281469 0.0 19.431489888567892 0.0 56 0.0 0.0010317787866281469 0.0 20.130004127115146 0.0 57 0.0 0.0010317787866281469 0.0 20.806851011143213 0.0 58 0.0 0.0010317787866281469 0.0 21.492983904250927 0.0 59 0.0 0.0010317787866281469 0.0 22.124432521667355 0.0 60 0.0 0.0010317787866281469 0.0 22.77754849360297 0.0 61 0.0 0.0010317787866281469 0.0 23.448204704911266 0.0 62 0.0 0.0010317787866281469 0.0 24.097193561700372 0.0 63 0.0 0.0010317787866281469 0.0 24.75546842756913 0.0 64 0.0 0.0010317787866281469 0.0 25.41683862979777 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGTA 20 0.0069329375 52.5 8 ATGTATG 20 0.0069329375 52.5 25 TGCTCTT 20 0.0069329375 52.5 46 GTATAGA 20 0.0069329375 52.5 36 ACGCTTC 50 1.698973E-4 35.000004 15 TATAACC 45 0.004289206 31.111109 3 TAAGAGA 45 0.004289206 31.111109 4 CTTATAA 60 4.951086E-4 29.166666 1 TCTCGGT 85 3.910027E-6 28.82353 43 ATCTCGG 120 7.264134E-8 26.25 42 AGATCTC 110 1.000908E-6 25.454544 40 TAGATCT 125 1.0786425E-7 25.199999 39 TTATACC 90 1.9111989E-4 23.333332 2 GTAGATC 90 1.9111989E-4 23.333332 35 AACCGTG 125 3.0438678E-6 22.4 70 TATACCC 100 3.8978958E-4 21.000002 3 TGTAGAT 105 5.4171146E-4 20.0 34 TTGAACC 255 5.2750693E-11 19.215687 70 GATCTCG 110 7.409784E-4 19.090908 41 GAACCGT 150 1.4735468E-5 18.666666 69 >>END_MODULE