FastQCFastQC Report
Wed 25 May 2016
SRR1780266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96920
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT89589.24267437061494No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC24052.4814279818406932No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG22762.3483285183656624No Hit
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG19632.0253817581510525Illumina PCR Primer Index 10 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC5060.5220800660338423No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT3110.32088320264135367Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT1890.19500619067271976No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1410.14548080891456872No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1210.12484523318200577No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATC150.002218744770.057
TCCGATT150.002218744770.058
CAAGGCA150.002218744770.04
AAGGCAC150.002218744770.05
TCTTTAT1200.058.3333321
CTTTATA1200.058.3333322
CTATACA252.3800888E-456.0000041
TTTATAC1300.053.8461533
GCGAATA200.006932937552.56
CTTTATG200.006932937552.53
CTCCGAT350.001250087940.057
TTATCGA350.001250087940.041
ATAGTAC350.001250087940.03
TATGCCG19200.036.2760443
CCGTCTT19050.036.19422547
CTGCTTG19150.036.18799254
TCGTATG19350.036.17571340
CGTATGC19400.036.08247441
CTTGAAA18850.036.0212257
GCTTGAA19050.036.01049856
Produced by FastQC (version 0.11.5)