##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780266_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96920 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.764052827073876 34.0 34.0 34.0 34.0 34.0 2 33.75244531572431 34.0 34.0 34.0 34.0 34.0 3 33.76273215022699 34.0 34.0 34.0 34.0 34.0 4 33.77980808914569 34.0 34.0 34.0 34.0 34.0 5 33.77443252166736 34.0 34.0 34.0 34.0 34.0 6 37.547606273215024 38.0 38.0 38.0 37.0 38.0 7 37.541260833677256 38.0 38.0 38.0 37.0 38.0 8 37.47186339248865 38.0 38.0 38.0 37.0 38.0 9 37.48597812628972 38.0 38.0 38.0 37.0 38.0 10-11 37.437623813454394 38.0 38.0 38.0 37.0 38.0 12-13 37.41092653735039 38.0 38.0 38.0 37.0 38.0 14-15 37.329328312009906 38.0 38.0 38.0 37.0 38.0 16-17 37.35183140734627 38.0 38.0 38.0 36.5 38.0 18-19 37.316209244737934 38.0 38.0 38.0 36.0 38.0 20-21 37.347513413124226 38.0 38.0 38.0 36.5 38.0 22-23 37.34172513413124 38.0 38.0 38.0 36.0 38.0 24-25 37.338511143210894 38.0 38.0 38.0 36.0 38.0 26-27 37.234812216260835 38.0 38.0 38.0 36.0 38.0 28-29 37.20021151465126 38.0 38.0 38.0 36.0 38.0 30-31 37.19679632686752 38.0 38.0 38.0 36.0 38.0 32-33 37.14272595955427 38.0 38.0 38.0 36.0 38.0 34-35 37.0884182831201 38.0 38.0 38.0 36.0 38.0 36-37 37.04178188196451 38.0 38.0 38.0 36.0 38.0 38-39 36.49623400742881 38.0 37.0 38.0 34.0 38.0 40-41 36.85601527032604 38.0 37.5 38.0 35.0 38.0 42-43 37.08215022699133 38.0 38.0 38.0 36.0 38.0 44-45 37.15127940569542 38.0 38.0 38.0 36.0 38.0 46-47 37.10387432934379 38.0 38.0 38.0 36.0 38.0 48-49 37.124195212546425 38.0 38.0 38.0 36.0 38.0 50-51 36.963150020635574 38.0 38.0 38.0 35.5 38.0 52-53 36.93659203466777 38.0 38.0 38.0 35.5 38.0 54-55 36.93493087082129 38.0 38.0 38.0 35.5 38.0 56-57 36.978673132480395 38.0 38.0 38.0 36.0 38.0 58-59 36.846909822534045 38.0 38.0 38.0 35.0 38.0 60-61 36.83892901361948 38.0 38.0 38.0 35.0 38.0 62-63 36.84152909616178 38.0 38.0 38.0 35.0 38.0 64-65 36.74582645480809 38.0 37.5 38.0 34.5 38.0 66-67 36.38108749484111 38.0 37.5 38.0 34.0 38.0 68-69 36.21411989269501 38.0 37.0 38.0 34.0 38.0 70-71 35.32857511349567 38.0 36.5 38.0 28.0 38.0 72-73 33.76953157243087 38.0 35.0 38.0 20.5 38.0 74-75 33.314068303755676 38.0 34.0 38.0 15.0 38.0 76 32.63249071399092 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10219810044774391 1101 2 0.08766937319301604 1101 3 0.1424263082612356 1101 4 -0.03566902187559151 1101 5 -0.00543517045075248 1101 6 -0.058127966457924174 1101 7 0.01056182966261332 1101 8 0.0632807023048656 1101 9 -0.00196874713413564 1101 10-11 0.05562781874191103 1101 12-13 0.14529978244002706 1101 14-15 0.30689657614721 1101 16-17 0.16442819160698008 1101 18-19 0.2670271828795805 1101 20-21 0.10141754002273018 1101 22-23 0.304072713679183 1101 24-25 0.14602783350086668 1101 26-27 0.19103988343500333 1101 28-29 0.003903434228497815 1101 30-31 0.08531338388849008 1101 32-33 -0.12188601339515515 1101 34-35 0.15576768086617676 1101 36-37 0.2865303195481701 1101 38-39 0.4867723962771109 1101 40-41 0.1712592997595479 1101 42-43 0.09513901092103794 1101 44-45 0.1117974312454777 1101 46-47 0.29259866570878756 1101 48-49 0.143816442708399 1101 50-51 0.04652154119078489 1101 52-53 -0.039987788676626224 1101 54-55 -0.07917345742240656 1101 56-57 -0.03796215877184039 1101 58-59 0.24475895464584596 1101 60-61 0.04160277278376867 1101 62-63 -0.09425254122641746 1101 64-65 -0.32264839223685016 1101 66-67 -0.2780554450338286 1101 68-69 -0.3081149738205582 1101 70-71 0.15457899895709915 1101 72-73 0.03174511588494511 1101 74-75 0.13076633236155288 1101 76 -0.7451952224515246 1102 1 -0.15217799357576212 1102 2 -0.24375723345864486 1102 3 -0.19056318911310655 1102 4 -0.08364976706188543 1102 5 -0.13606157705240918 1102 6 -0.1745633252976404 1102 7 -0.25534839789428077 1102 8 -0.225572761060981 1102 9 -0.20289397844196344 1102 10-11 -0.16677153109554865 1102 12-13 0.04249283070208776 1102 14-15 -0.06982260364773651 1102 16-17 -0.11426648206144563 1102 18-19 -0.02532715570506383 1102 20-21 0.01647442924504361 1102 22-23 0.03137954038585633 1102 24-25 -0.22855018600401422 1102 26-27 7.423090414064859E-4 1102 28-29 0.13570200887215123 1102 30-31 -0.0025367036333889814 1102 32-33 -0.2311164460033055 1102 34-35 -0.059895609956527096 1102 36-37 -0.057368155070491866 1102 38-39 0.030502078697715262 1102 40-41 0.1828715778290686 1102 42-43 -0.040973142117223915 1102 44-45 0.03287144975010392 1102 46-47 -0.20008700570905802 1102 48-49 -0.0673613517402103 1102 50-51 -0.4065917371287995 1102 52-53 -0.11213082443556033 1102 54-55 0.08039768536329461 1102 56-57 -0.020739103007898052 1102 58-59 0.019843350744864097 1102 60-61 0.19900149746494122 1102 62-63 0.15527859155677248 1102 64-65 -0.2616175095161637 1102 66-67 -0.0532805012978983 1102 68-69 0.1354646710906664 1102 70-71 -0.6522477077195745 1102 72-73 -0.2490907180735391 1102 74-75 0.25870456692019417 1102 76 -0.052271991643827675 1103 1 -0.0020844357035301186 1103 2 0.13724606334256606 1103 3 -0.11268739832632946 1103 4 -0.04943431664755593 1103 5 -0.1089297941066718 1103 6 -0.018098921840454807 1103 7 0.04394769049563507 1103 8 -0.06385129621200747 1103 9 -0.1256864581710886 1103 10-11 -0.23304169058944524 1103 12-13 -0.10635328887377682 1103 14-15 0.02068349988963547 1103 16-17 0.13751557264763647 1103 18-19 0.02102854241486085 1103 20-21 0.11582397927622168 1103 22-23 0.07644324884170572 1103 24-25 0.07252795709498372 1103 26-27 0.18346974377364944 1103 28-29 0.2525825221039142 1103 30-31 0.12127154727991751 1103 32-33 0.15498798240264477 1103 34-35 -0.1332494369190158 1103 36-37 -0.04179745204991292 1103 38-39 0.22189475445286888 1103 40-41 0.17832733832091918 1103 42-43 0.027123623453476853 1103 44-45 -0.1515043855880478 1103 46-47 -0.08229318943890718 1103 48-49 0.0925557827158201 1103 50-51 0.10964723640769591 1103 52-53 0.12535024123314997 1103 54-55 -0.2151115367698253 1103 56-57 -0.13728548098169568 1103 58-59 0.08446510963285903 1103 60-61 -0.0352495940435702 1103 62-63 -0.13604280201000307 1103 64-65 0.2500326731444389 1103 66-67 0.2705633907095901 1103 68-69 0.5734046158469752 1103 70-71 -0.03587953521667231 1103 72-73 0.3400120173804382 1103 74-75 0.43463791807407404 1103 76 0.558660913996377 1104 1 0.007011410074369451 1104 2 0.06363457512341597 1104 3 0.14893507127876404 1104 4 0.04138838223921937 1104 5 0.09841928725816018 1104 6 0.12681615343031183 1104 7 -0.1050401515413526 1104 8 -0.13480794387243122 1104 9 0.006286269375898712 1104 10-11 0.10709467167561826 1104 12-13 -0.16302508420970696 1104 14-15 0.009588981532019147 1104 16-17 0.1437906503318942 1104 18-19 0.10538760960043447 1104 20-21 0.033901029989714004 1104 22-23 0.022132149798054 1104 24-25 -0.01572286500053366 1104 26-27 0.1152207317966969 1104 28-29 -0.12482719823277222 1104 30-31 0.018781125823977618 1104 32-33 0.03713417964523558 1104 34-35 0.06882013197107995 1104 36-37 -0.016365286243051003 1104 38-39 0.08783284839801553 1104 40-41 -0.030718729196507866 1104 42-43 0.05076829717961573 1104 44-45 0.10700317165695594 1104 46-47 -0.2879829954545343 1104 48-49 -0.19787774067997077 1104 50-51 -0.4229348977220937 1104 52-53 -0.18356157582419996 1104 54-55 -0.42286789481128295 1104 56-57 -0.3774655877786941 1104 58-59 -0.0647565393851437 1104 60-61 -0.09947863437904658 1104 62-63 0.05900501528869029 1104 64-65 -0.09844531883069862 1104 66-67 -0.44775953444200667 1104 68-69 -0.3759648595203302 1104 70-71 -0.5328098758206536 1104 72-73 -0.47484302229133135 1104 74-75 -0.5243327278365655 1104 76 -0.6032089384789572 1105 1 -0.12192185539672806 1105 2 0.008726039450834833 1105 3 0.04977636345564207 1105 4 -0.07775475718774061 1105 5 -0.06076756343493628 1105 6 0.14126182870949577 1105 7 0.07365210318155846 1105 8 0.14584233605931018 1105 9 0.07689792770948145 1105 10-11 0.060120030197822416 1105 12-13 0.1175952564986531 1105 14-15 0.15084972222840065 1105 16-17 0.097489641380065 1105 18-19 0.1780875287645287 1105 20-21 0.22642520591259085 1105 22-23 0.21364587693197024 1105 24-25 0.006439645103952785 1105 26-27 0.12109630438434493 1105 28-29 0.23206331402734293 1105 30-31 -0.004983132731155138 1105 32-33 -0.16664714426722327 1105 34-35 0.07986770539702093 1105 36-37 0.25482419910169796 1105 38-39 -0.06294856533211401 1105 40-41 -0.13769905912674574 1105 42-43 -0.051915754196095065 1105 44-45 0.18292155733403348 1105 46-47 -0.3031023032596849 1105 48-49 0.022235429584398503 1105 50-51 -0.143991153522812 1105 52-53 -0.05203856753102798 1105 54-55 -0.25501363895067186 1105 56-57 6.892578846091624E-4 1105 58-59 0.09630899757482325 1105 60-61 0.049828272599789614 1105 62-63 0.23490543743174186 1105 64-65 0.0295149218806543 1105 66-67 -0.5613714023501544 1105 68-69 -0.016611846137493558 1105 70-71 -0.551664101742027 1105 72-73 -0.2246559021452299 1105 74-75 -0.544092615265086 1105 76 -0.1875477307306852 1106 1 8.333361797596694E-4 1106 2 0.0984013831185706 1106 3 0.08206650677239935 1106 4 0.10335082924103745 1106 5 0.12440353922666247 1106 6 0.05707054470832418 1106 7 0.09238565100255869 1106 8 0.21470750131230432 1106 9 0.16800643897008172 1106 10-11 0.15233997755085227 1106 12-13 0.1274958122167007 1106 14-15 -0.08745826256246403 1106 16-17 -0.05281746596126169 1106 18-19 0.16659293970339917 1106 20-21 0.05796220503514604 1106 22-23 0.07435807348067414 1106 24-25 0.2299918533519758 1106 26-27 0.12174707946478236 1106 28-29 0.1273835831118717 1106 30-31 0.2514734335162885 1106 32-33 0.3099688253145274 1106 34-35 0.3262701622557529 1106 36-37 0.20241121284841057 1106 38-39 0.2823058950364228 1106 40-41 0.4804714585684806 1106 42-43 0.20652238561086733 1106 44-45 0.1101224932802154 1106 46-47 0.1986160354358404 1106 48-49 0.2781216536265134 1106 50-51 0.3542758836225488 1106 52-53 0.33526035028119594 1106 54-55 0.20122356491010152 1106 56-57 0.36023883378398835 1106 58-59 0.2438594509237575 1106 60-61 0.3236437157118104 1106 62-63 0.16865068458002952 1106 64-65 0.2731022281105453 1106 66-67 0.7461381033532675 1106 68-69 0.8650860187352762 1106 70-71 0.534603812852886 1106 72-73 0.7900267536020706 1106 74-75 0.7623420147436875 1106 76 0.9629933631564143 1107 1 -0.15301281766639363 1107 2 -0.11698323226604401 1107 3 -0.04101041630452329 1107 4 -0.0389568630666588 1107 5 0.059018923842046433 1107 6 0.11476285240063078 1107 7 0.06930872792563747 1107 8 -0.10452326791845934 1107 9 -0.05507048410684234 1107 10-11 -0.10727540706453453 1107 12-13 -0.24365803393715169 1107 14-15 0.08754173743753313 1107 16-17 0.08179791865412511 1107 18-19 -0.16033013721967393 1107 20-21 -0.25934548727255446 1107 22-23 -0.07179577267317683 1107 24-25 -0.13154660818648267 1107 26-27 -0.1205606128429082 1107 28-29 -0.030308724580443425 1107 30-31 -0.2966034895606384 1107 32-33 0.07727651762221655 1107 34-35 -0.014114453128861726 1107 36-37 -0.032204171766970546 1107 38-39 -0.0600017972712763 1107 40-41 -0.28491315681613116 1107 42-43 -0.18963146054298363 1107 44-45 -0.030262122104396383 1107 46-47 -0.26484550302569687 1107 48-49 -0.023801423296561097 1107 50-51 -0.045724116377449775 1107 52-53 0.1064141964350398 1107 54-55 0.1781466418331803 1107 56-57 -0.23206885852369652 1107 58-59 0.1419363740006787 1107 60-61 -0.2013562842881882 1107 62-63 0.09365068458003378 1107 64-65 0.2596406896490109 1107 66-67 0.384599641814809 1107 68-69 0.3535475571968121 1107 70-71 0.10960381285287468 1107 72-73 -0.6772809387056284 1107 74-75 -0.8299656775640045 1107 76 -1.2523912522282004 1108 1 -0.15917255830889587 1108 2 0.08360621659927148 1108 3 0.16317467063918656 1108 4 -0.05326573427208103 1108 5 -0.1127612117311898 1108 6 -0.3284437494266612 1108 7 -0.028849244370263705 1108 8 -0.10216547245721586 1108 9 -0.18315772253890827 1108 10-11 -0.016566594804004353 1108 12-13 -0.6465831739312478 1108 14-15 -0.4275923621793254 1108 16-17 -0.05213959976615712 1108 18-19 0.13022394471371257 1108 20-21 0.05260558847162855 1108 22-23 -0.2032502377483283 1108 24-25 -0.2109969471195754 1108 26-27 0.01105595067019749 1108 28-29 0.016950338195862003 1108 30-31 -0.1737476098082027 1108 32-33 -0.21091240073911877 1108 34-35 -0.08535671661250888 1108 36-37 -0.018808946302783625 1108 38-39 -0.20530831068123234 1108 40-41 -0.32358837049134337 1108 42-43 0.027123623453483958 1108 44-45 -0.28943542007079515 1108 46-47 -0.11294453043507247 1108 48-49 0.12895425014876594 1108 50-51 -0.05510372144671294 1108 52-53 8.291684362262686E-4 1108 54-55 -0.17296594290009892 1108 56-57 -0.06831996374032201 1108 58-59 -0.3005923616315016 1108 60-61 -0.1750963373385943 1108 62-63 0.013382485346312478 1108 64-65 0.393710834063981 1108 66-67 0.31462469339158616 1108 68-69 0.3243624702531065 1108 70-71 0.36641931535803707 1108 72-73 -0.06803395963105885 1108 74-75 0.21816282228863315 1108 76 0.4245612971381405 1109 1 -0.023450223397922798 1109 2 0.07607860785461895 1109 3 0.11796394266983867 1109 4 0.10485912486697657 1109 5 0.17342314706979778 1109 6 -0.11117983236557905 1109 7 0.2265485469301609 1109 8 0.08341036707399496 1109 9 0.20435636355529851 1109 10-11 -0.08717736784885233 1109 12-13 0.12425297662092305 1109 14-15 -0.03892885079775965 1109 16-17 0.1162624737069109 1109 18-19 0.04487348268983027 1109 20-21 -0.028350012159428672 1109 22-23 0.06564766624084939 1109 24-25 -0.14847722658768703 1109 26-27 -0.08824537905709207 1109 28-29 0.0611694051329863 1109 30-31 0.13348700817692105 1109 32-33 -0.106697841352144 1109 34-35 0.11518418940507757 1109 36-37 0.018851635171188263 1109 38-39 0.15421388900323763 1109 40-41 -0.03796745545866287 1109 42-43 -0.024397674720958662 1109 44-45 -0.16076740113908983 1109 46-47 0.020524029402658073 1109 48-49 -0.12515888935990915 1109 50-51 -0.017330451219081056 1109 52-53 0.023231692664296588 1109 54-55 0.12011496762502816 1109 56-57 -0.021058300454321 1109 58-59 -0.2384557828620686 1109 60-61 0.02111732053835169 1109 62-63 -0.195565001694483 1109 64-65 -0.4206006376511411 1109 66-67 -0.5121952299800654 1109 68-69 0.06957319822245012 1109 70-71 0.2560970255225641 1109 72-73 0.2244158938735623 1109 74-75 0.5335335682881777 1109 76 0.5106555049362029 1110 1 0.10149959905074724 1110 2 -0.141089834325399 1110 3 -0.07941138541415427 1110 4 0.013829327121108292 1110 5 -0.14567520093081754 1110 6 -0.24719959460118446 1110 7 -0.08535511217127123 1110 8 0.06204244982836116 1110 9 -0.10788695897056044 1110 10-11 -0.14290532832438174 1110 12-13 -0.1295605172805807 1110 14-15 -0.177418892483729 1110 16-17 -0.005815648880705737 1110 18-19 0.14278918440358268 1110 20-21 0.2169718961314473 1110 22-23 -0.20424580295784978 1110 24-25 0.3031747727947476 1110 26-27 0.129000259355756 1110 28-29 0.10351925846014609 1110 30-31 0.17429293684759983 1110 32-33 0.2457955969680654 1110 34-35 0.007796509923835515 1110 36-37 0.3674929814734895 1110 38-39 0.018935210602741392 1110 40-41 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-0.5313715491563826 2115 68-69 -0.3911732625240063 2115 70-71 -0.597071268822198 2115 72-73 -0.7275259639506473 2115 74-75 -0.4769214245197517 2115 76 -0.7260770259139733 2116 1 0.006736735106414926 2116 2 0.008180751037478728 2116 3 -0.016144584461947886 2116 4 -0.15729317791459607 2116 5 -0.050790259222949885 2116 6 0.05799481423724728 2116 7 0.12449656334603532 2116 8 0.030241192427396868 2116 9 0.0025323781471868756 2116 10-11 0.04446877898197954 2116 12-13 0.23629128031448943 2116 14-15 0.2654487141817157 2116 16-17 0.046586231116855004 2116 18-19 0.12002168150423387 2116 20-21 0.10562171606674298 2116 22-23 0.13021674968818076 2116 24-25 0.13579089926730603 2116 26-27 0.02206013849876598 2116 28-29 -0.04453054926738531 2116 30-31 0.16025399404102814 2116 32-33 0.22043394159359764 2116 34-35 0.07188733577990547 2116 36-37 -0.1348875349154568 2116 38-39 -0.2253118747906555 2116 40-41 -0.16208966248100865 2116 42-43 0.1256935245494475 2116 44-45 0.07356912416870642 2116 46-47 0.30960291677155993 2116 48-49 -0.11968693313556145 2116 50-51 -0.07713437278770385 2116 52-53 0.16887692750242422 2116 54-55 0.2063666776113564 2116 56-57 0.27189655590682804 2116 58-59 0.13999838950455512 2116 60-61 0.020706923821528278 2116 62-63 0.12233285512267145 2116 64-65 0.02714218040631522 2116 66-67 0.22191329715171548 2116 68-69 0.3641468416333069 2116 70-71 0.3613181837532977 2116 72-73 -0.3091682374533846 2116 74-75 -0.46610163463019205 2116 76 0.052379171146874626 2117 1 -0.06682757433829778 2117 2 0.020536390967869522 2117 3 -0.12829456073152556 2117 4 0.0876522421138759 2117 5 -0.004167889344763864 2117 6 -0.18665002044080836 2117 7 -0.03563632701941799 2117 8 -0.44832232600323607 2117 9 0.028445999409640876 2117 10-11 0.02690826640484545 2117 12-13 -0.23569100097011386 2117 14-15 -0.03388683399104764 2117 16-17 -0.2901801033238982 2117 18-19 -0.17673516076755647 2117 20-21 -0.08909430987851152 2117 22-23 -0.10386013688039952 2117 24-25 -0.4558401717657574 2117 26-27 -0.24618855632800063 2117 28-29 -0.9650810950670916 2117 30-31 -0.31693315989031134 2117 32-33 -0.7481944399916003 2117 34-35 -0.3083452223596339 2117 36-37 -0.31972379500087555 2117 38-39 -0.5054099605365749 2117 40-41 -0.4546541301285387 2117 42-43 -0.20430963950687442 2117 44-45 -0.17449289133517 2117 46-47 -0.22073247319838174 2117 48-49 -0.2504890214127329 2117 50-51 -0.21216838639315228 2117 52-53 -0.7063801520735922 2117 54-55 -0.1581563408983797 2117 56-57 -0.6262603812866487 2117 58-59 -0.3315330137544237 2117 60-61 -0.5760815590134669 2117 62-63 -0.201247274603638 2117 64-65 -0.354174067022889 2117 66-67 -0.5870251619528588 2117 68-69 -0.8780458493965924 2117 70-71 -0.7778765639132104 2117 72-73 -0.9903500124889817 2117 74-75 -0.4541415017398265 2117 76 -0.7881840308781243 2118 1 -0.03270512535870296 2118 2 -0.20713707841989049 2118 3 -0.07670272399683142 2118 4 -0.2255722476820381 2118 5 0.004403539226665032 2118 6 0.06583977547755637 2118 7 0.01807795869486739 2118 8 -0.12113865253384404 2118 9 -0.05783971487607431 2118 10-11 -0.050275407064532374 2118 12-13 -0.019119572398686557 2118 14-15 0.06954173743753245 2118 16-17 -0.04189438903818399 2118 18-19 -0.23571475260428798 2118 20-21 -0.06811471804178382 2118 22-23 -0.10741115728855988 2118 24-25 0.19191493027506112 2118 26-27 0.09136246408016291 2118 28-29 -0.02769333996505452 2118 30-31 0.0027811258239722747 2118 32-33 -0.1860311746854748 2118 34-35 -0.5483452223596288 2118 36-37 -0.020050325613119924 2118 38-39 -0.20700179727126766 2118 40-41 -0.10114392604690181 2118 42-43 0.0274454625339402 2118 44-45 0.10150710866482626 2118 46-47 -0.09069165687184721 2118 48-49 0.1134293459342075 2118 50-51 0.10019896054562594 2118 52-53 0.03872188874272808 2118 54-55 0.1217620264485646 2118 56-57 0.046392679937838466 2118 58-59 -0.1740636259993238 2118 60-61 -0.10151013044203694 2118 62-63 0.06965068458003287 2118 64-65 -0.23405161804330277 2118 66-67 -0.2168618966467264 2118 68-69 -0.16176013511087461 2118 70-71 -0.41555003330096696 2118 72-73 -0.9978194002440901 2118 74-75 -0.894427216025548 2118 76 -1.2277758676128165 2119 1 -0.039331079557179294 2119 2 -0.0035950936870605688 2119 3 -0.03114547208843277 2119 4 0.08902317216529099 2119 5 0.029747050677045195 2119 6 -0.004908316125494139 2119 7 -0.13770830084711605 2119 8 0.17498348487073656 2119 9 0.03324425458957592 2119 10-11 -0.010519681873695674 2119 12-13 0.050895694776883715 2119 14-15 -0.4826872701960525 2119 16-17 -0.05910049590841737 2119 18-19 -0.1596842182531475 2119 20-21 -4.2006155323548455E-4 2119 22-23 0.1529399877496047 2119 24-25 -0.016940031556998747 2119 26-27 -0.04535509317174302 2119 28-29 0.3043066600349462 2119 30-31 0.08175822506061792 2119 32-33 -0.10758842659387113 2119 34-35 -0.12361239793214907 2119 36-37 -0.19444727217801017 2119 38-39 -0.25790256063005046 2119 40-41 -0.1959378191766703 2119 42-43 -0.29976064433628835 2119 44-45 -0.3405234256863139 2119 46-47 -0.179760359161925 2119 48-49 0.011459880285357826 2119 50-51 0.004290563599063546 2119 52-53 -0.1001330730893244 2119 54-55 0.005319278356957113 2119 56-57 -0.2626912895278153 2119 58-59 -0.006918587831378886 2119 60-61 0.05134483139001844 2119 62-63 -0.36779969709935756 2119 64-65 -0.27858596918833456 2119 66-67 -0.17275502641772533 2119 68-69 -0.14734792137041808 2119 70-71 0.2853965315845315 2119 72-73 -0.018781232305151718 2119 74-75 -0.060411948849974806 2119 76 -1.277699531734953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 2.0 21 1.0 22 1.0 23 9.0 24 22.0 25 38.0 26 97.0 27 162.0 28 295.0 29 497.0 30 825.0 31 1229.0 32 1993.0 33 3313.0 34 5623.0 35 9697.0 36 14554.0 37 58561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.61906727197689 14.652290548906313 12.019191085431284 37.709451093685516 2 19.408790755262075 17.044985555096986 36.77569129178704 26.770532397853902 3 18.795914156004955 15.578827899298389 33.442014032191494 32.18324391250516 4 15.508666941807677 23.89186958316137 29.10751134956665 31.4919521254643 5 16.64775072224515 27.52063557573256 38.87639290136195 16.95522080066034 6 30.07738340899711 36.21027651671482 15.134131242261658 18.578208832026412 7 27.967395790342554 22.5660338423442 19.98245976062732 29.484110606685928 8 23.857820883202642 36.621956252579444 14.594510936855137 24.925711927362773 9 21.663227404044573 14.976269087907554 14.260214609987617 49.100288898060256 10-11 27.203363598844412 25.66859265373504 21.85513825835741 25.272905489063145 12-13 25.54219975237309 16.6018365662402 24.957697069748246 32.89826661163847 14-15 22.740404457284356 15.671172100701611 18.784564589352044 42.80385885266199 16-17 30.100598431696245 23.369273627734213 16.4919521254643 30.03817581510524 18-19 32.73008666941808 21.24019810152703 16.78085018572018 29.24886504333471 20-21 26.18190260008254 18.465744944283948 16.900536524969045 38.451815930664466 22-23 24.352042921997523 24.795707800247627 16.640528270738752 34.211721007016095 24-25 31.61370202228642 23.72833264548081 17.3643210895584 27.293644242674368 26-27 32.35916219562526 26.45377631035906 17.276619892695006 23.910441601320677 28-29 25.284255055716052 26.6054477919934 18.49411886091622 29.61617829137433 30-31 29.956149401568304 21.149401568303755 23.195934791580687 25.698514238547254 32-33 29.645583986793234 23.85163021048287 22.01867519603797 24.484110606685928 34-35 26.260317787866285 25.18572018159307 24.346884028064384 24.20707800247627 36-37 24.16167973586463 23.11390837804375 28.549319026000823 24.175092860090796 38-39 22.110503508047874 21.28043747420553 28.089661576557983 28.51939744118861 40-41 23.16756087494841 16.5615971935617 27.736277342137843 32.534564589352044 42-43 28.771151465125875 16.359368551382584 26.70862567065621 28.160854312835326 44-45 22.062525794469664 23.367210070160958 29.96749896822121 24.602765167148164 46-47 26.69830788278993 18.9991745769707 26.37794056954189 27.924576970697483 48-49 26.46409409822534 17.30344614114734 21.960379694593477 34.272080066033844 50-51 23.05561287659926 18.143829962855964 27.060978126289726 31.739579034255055 52-53 18.929013619479985 17.888980602558814 36.150433347090384 27.03157243087082 54-55 19.536731324803963 19.094098225340485 30.588629797771354 30.78054065208419 56-57 24.601733388361534 20.182108955839865 23.719046636401156 31.49711101939744 58-59 20.741848947585638 20.54426330994635 30.969872059430458 27.74401568303756 60-61 25.205839867932315 25.565414775072227 24.02754849360297 25.20119686339249 62-63 19.917973586463063 35.35751134956665 19.153941394964917 25.570573669005363 64-65 18.84234420140322 37.37670243499794 18.17633099463475 25.60462236896409 66-67 19.083780437474203 37.590796533223276 17.84203466776723 25.483388361535287 68-69 18.650949236483697 38.09327280231118 18.260420965744945 24.995356995460174 70-71 19.096161782913743 36.61886091621957 18.683450268262487 25.60152703260421 72-73 19.22410235245563 33.49308708212959 19.83027238959967 27.452538175815107 74-75 19.386607511349567 32.62020222864218 21.045191910854314 26.94799834915394 76 19.61411473380107 32.70738753611226 20.936855138258355 26.741642591828313 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 3.5 19 6.0 20 12.5 21 18.0 22 22.0 23 33.5 24 40.5 25 40.0 26 83.5 27 134.5 28 180.5 29 219.0 30 253.5 31 360.5 32 553.5 33 674.0 34 752.0 35 911.5 36 1186.5 37 1380.0 38 3605.5 39 6946.0 40 7276.0 41 5595.5 42 4700.0 43 4171.5 44 3486.0 45 3218.0 46 3107.0 47 3035.0 48 2835.0 49 2628.5 50 2550.0 51 2308.0 52 1928.5 53 1793.5 54 1796.0 55 1853.0 56 1895.5 57 2004.5 58 2128.0 59 2170.5 60 2418.0 61 2785.0 62 2947.0 63 2937.0 64 3093.5 65 3287.5 66 3365.5 67 3416.0 68 3357.5 69 3168.0 70 2898.0 71 2759.0 72 2552.0 73 2116.0 74 1621.5 75 1356.0 76 1057.5 77 576.5 78 277.0 79 160.0 80 95.0 81 20.0 82 6.0 83 2.0 84 1.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 96920.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.48514238547255 #Duplication Level Percentage of deduplicated Percentage of total 1 64.55036383728446 31.9428394552208 2 21.83232209503555 21.607511349566654 3 7.376827005275119 10.951300041271152 4 2.787681658013803 5.51795295088733 5 1.2843768895560976 3.1778786628146927 6 0.7589499801922395 2.253404869995873 7 0.4649611142386522 1.6106066859265373 8 0.2522883175913763 0.9987618654560462 9 0.21475782406538646 0.9564589352042923 >10 0.43785575780321506 2.908584399504746 >50 0.02085027418110548 0.6685926537350393 >100 0.008340109672442192 0.786215435410648 >500 0.002085027418110548 0.5220800660338423 >1k 0.006255082254331645 6.8551382583574085 >5k 0.002085027418110548 9.24267437061494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 8958 9.24267437061494 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 2405 2.4814279818406932 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 2276 2.3483285183656624 No Hit CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG 1963 2.0253817581510525 Illumina PCR Primer Index 10 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 506 0.5220800660338423 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 311 0.32088320264135367 Illumina PCR Primer Index 10 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT 189 0.19500619067271976 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 141 0.14548080891456872 No Hit CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 121 0.12484523318200577 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0010317787866281469 0.0 2 0.0 0.0 0.0 0.004127115146512587 0.0 3 0.0 0.0 0.0 0.005158893933140734 0.0 4 0.0 0.0 0.0 0.01650846058605035 0.0 5 0.0 0.0 0.0 0.020635575732562937 0.0 6 0.0 0.0 0.0 0.020635575732562937 0.0 7 0.0 0.0 0.0 0.02269913330581923 0.0 8 0.0 0.0 0.0 0.02373091209244738 0.0 9 0.0 0.0 0.0 0.035080478745357 0.0 10 0.0 0.0 0.0 0.04023937267849773 0.0 11 0.0 0.0 0.0 0.04230293025175402 0.0 12 0.0 0.0 0.0 0.04333470903838217 0.0 13 0.0 0.0 0.0 0.04849360297152291 0.0 14 0.0 0.0 0.0 0.0505571605447792 0.0 15 0.0 0.0 0.0 0.0505571605447792 0.0 16 0.0 0.0 0.0 0.052620718118035495 0.0 17 0.0 0.0 0.0 0.055716054477919934 0.0 18 0.0 0.0 0.0 0.05984316962443252 0.0 19 0.0 0.0 0.0 0.06706562113082955 0.0 20 0.0 0.0 0.0 0.07841518778373917 0.0 21 0.0 0.0 0.0 0.08460586050350805 0.0 22 0.0 0.0 0.0 0.09492364836978952 0.0 23 0.0 0.0 0.0 0.1011143210895584 0.0 24 0.0 0.0 0.0 0.10730499380932729 0.0 25 0.0 0.0020635575732562937 0.0 0.11968633924886504 0.0 26 0.0 0.0020635575732562937 0.0 0.14548080891456872 0.0 27 0.0 0.0020635575732562937 0.0 0.25381758151052414 0.0 28 0.0 0.0020635575732562937 0.0 0.3776310359059018 0.0 29 0.0 0.0020635575732562937 0.0 0.540652084193149 0.0 30 0.0 0.0020635575732562937 0.0 0.7170862567065621 0.0 31 0.0 0.0020635575732562937 0.0 0.8997111019397441 0.0 32 0.0 0.0020635575732562937 0.0 1.110193974411886 0.0 33 0.0 0.0020635575732562937 0.0 1.4558398679323152 0.0 34 0.0 0.0020635575732562937 0.0 1.833470903838217 0.0 35 0.0 0.0020635575732562937 0.0 2.4071399092034667 0.0 36 0.0 0.0020635575732562937 0.0 3.208832026413537 0.0 37 0.0 0.0020635575732562937 0.0 4.190053652496904 0.0 38 0.0 0.0020635575732562937 0.0 5.176434172513413 0.0 39 0.0 0.0020635575732562937 0.0 6.165910028889806 0.0 40 0.0 0.0020635575732562937 0.0 7.026413536937681 0.0 41 0.0 0.0020635575732562937 0.0 7.972554684275691 0.0 42 0.0 0.0020635575732562937 0.0 8.861947998349153 0.0 43 0.0 0.0020635575732562937 0.0 9.831820057779613 0.0 44 0.0 0.0020635575732562937 0.0 10.799628559636814 0.0 45 0.0 0.0020635575732562937 0.0 11.80458109781263 0.0 46 0.0 0.0020635575732562937 0.0 12.716673545191911 0.0 47 0.0 0.0020635575732562937 0.0 13.658687577383409 0.0 48 0.0 0.0020635575732562937 0.0 14.518159306644655 0.0 49 0.0 0.0020635575732562937 0.0 15.20119686339249 0.0 50 0.0 0.006190672719768881 0.0 15.980189847296739 0.0 51 0.0 0.006190672719768881 0.0 16.712752785802724 0.0 52 0.0 0.006190672719768881 0.0 17.492777548493603 0.0 53 0.0 0.006190672719768881 0.0 18.260420965744945 0.0 54 0.0 0.006190672719768881 0.0 19.023937267849774 0.0 55 0.0 0.006190672719768881 0.0 19.720387948823774 0.0 56 0.0 0.006190672719768881 0.0 20.42818819645068 0.0 57 0.0 0.006190672719768881 0.0 21.112257531985144 0.0 58 0.0 0.006190672719768881 0.0 21.806644655385885 0.0 59 0.0 0.006190672719768881 0.0 22.442220387948822 0.0 60 0.0 0.006190672719768881 0.0 23.104622368964094 0.0 61 0.0 0.006190672719768881 0.0 23.773215022699134 0.0 62 0.0 0.006190672719768881 0.0 24.431489888567892 0.0 63 0.0 0.006190672719768881 0.0 25.101114321089558 0.0 64 0.0 0.006190672719768881 0.0 25.77280231118448 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAATC 15 0.0022187447 70.0 57 TCCGATT 15 0.0022187447 70.0 58 CAAGGCA 15 0.0022187447 70.0 4 AAGGCAC 15 0.0022187447 70.0 5 TCTTTAT 120 0.0 58.333332 1 CTTTATA 120 0.0 58.333332 2 CTATACA 25 2.3800888E-4 56.000004 1 TTTATAC 130 0.0 53.846153 3 GCGAATA 20 0.0069329375 52.5 6 CTTTATG 20 0.0069329375 52.5 3 CTCCGAT 35 0.0012500879 40.0 57 TTATCGA 35 0.0012500879 40.0 41 ATAGTAC 35 0.0012500879 40.0 3 TATGCCG 1920 0.0 36.27604 43 CCGTCTT 1905 0.0 36.194225 47 CTGCTTG 1915 0.0 36.187992 54 TCGTATG 1935 0.0 36.175713 40 CGTATGC 1940 0.0 36.082474 41 CTTGAAA 1885 0.0 36.02122 57 GCTTGAA 1905 0.0 36.010498 56 >>END_MODULE