FastQCFastQC Report
Wed 25 May 2016
SRR1780265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458683
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATGC301.3056894E-436.6388663
GCTAGCT250.002351216535.20017623
CTATTGC353.2291026E-431.4047458
TGCTAGC300.005741201329.3334822
AATAACC500.002579575921.9977127
ATGCAGT500.002584482521.99051331
GAATAGA500.00258939721.9833221
TAACGAA550.00445391220.01975620
TATGCAG550.004482140319.99791730
TTGTATA600.007437731618.3194332
ATTACAC750.001295785717.5866574
CAAGGAA750.001295785717.5866574
GTACAAG800.0019959116.487491
AAGGAAG800.0019959116.487495
ACAAGGA1206.435368E-616.487493
TCTACAC1259.48265E-615.8279913
GAACTGT1004.918836E-415.406837
TAGATCT1503.2248645E-614.67314339
TTTTTTA1057.1524933E-414.66513926
TCATGGT1351.9647587E-514.6555472
Produced by FastQC (version 0.11.5)