FastQCFastQC Report
Wed 25 May 2016
SRR1780265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458683
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT203654.439885498263506No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC57471.252935033563485No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG53271.1613685268475178No Hit
CACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCTG43180.9413908952370156RNA PCR Primer, Index 34 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC10950.23872696393805745No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT5940.12950120235543938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG5350.037.418844
GTCTTCT5450.036.73221643
CTTTATA2050.035.4129682
GAACATT250.002351818835.1983441
CCGTCTT5800.034.8949141
ATGCCGT28400.033.15336644
CGTCTTC6400.031.62351242
TTTATAC2650.031.5456853
TCTTTAT2350.030.9562841
CTTAATA502.3202629E-630.8624741
TTAATAC502.3581015E-630.798552
GCCGTCT6600.030.33190540
TTACTCC300.00574266629.3319518
GATTACT300.00574266629.3319516
TATGCCG33250.028.25130343
TTATATA407.0273684E-427.4987052
TAATGGC407.0273684E-427.49870537
TGCCGTC7900.025.61904539
CTATTAA450.001380495324.4940281
ACACATC42650.022.4889776
Produced by FastQC (version 0.11.5)