FastQCFastQC Report
Wed 25 May 2016
SRR1780264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97523
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTT150.002218785770.0000119
GTTTACC200.00693306552.50000436
TGGAATA200.00693306552.50000436
ATTCACG200.00693306552.50000427
GCCTAAA200.00693306552.50000411
TTTTGGT200.00693306552.50000423
CGCTTTG200.00693306552.50000453
CAAAGAG200.00693306552.50000456
AACCGTT305.852823E-446.66666870
TTGTGCC305.852823E-446.66666838
CATAAGG350.001250125840.029
CGACGAT753.4855475E-837.33333222
CACGACG400.002408626735.00000412
TATAGTG400.002408626735.0000045
TCATAAC400.002408626735.0000042
ATTGCCA400.002408626735.00000446
GGGTGTG450.00428933531.1111134
TGGCTAT500.007178555727.99999842
GACGATG700.001218519225.023
CTCATAA901.9113172E-423.3333341
Produced by FastQC (version 0.11.5)