##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780264_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 97523 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.12070998636219 34.0 27.0 34.0 12.0 34.0 2 29.185607497718486 34.0 27.0 34.0 12.0 34.0 3 28.97673369359023 34.0 27.0 34.0 11.0 34.0 4 28.857336218122903 34.0 23.0 34.0 11.0 34.0 5 28.84939962880551 34.0 27.0 34.0 11.0 34.0 6 31.530674815171807 37.0 27.0 38.0 11.0 38.0 7 31.93737887472699 37.0 28.0 38.0 12.0 38.0 8 31.868841196435714 37.0 29.0 38.0 11.0 38.0 9 32.1730361043036 37.0 31.0 38.0 12.0 38.0 10-11 31.810121714877518 37.0 29.5 38.0 11.0 38.0 12-13 31.891051341734773 37.0 31.0 38.0 11.0 38.0 14-15 31.75224306061134 37.0 28.0 38.0 11.0 38.0 16-17 31.777252545553356 37.0 28.0 38.0 11.0 38.0 18-19 31.824282476954153 37.0 28.5 38.0 11.0 38.0 20-21 31.830178521989684 37.0 29.5 38.0 11.0 38.0 22-23 31.797827179229515 37.0 29.5 38.0 11.0 38.0 24-25 31.79900638823662 37.0 29.5 38.0 11.0 38.0 26-27 31.75824677255622 37.0 28.0 38.0 11.0 38.0 28-29 31.79073141720415 37.5 28.5 38.0 11.0 38.0 30-31 31.80445638464772 37.5 28.0 38.0 11.0 38.0 32-33 31.87743916819622 38.0 30.0 38.0 11.0 38.0 34-35 31.830342585851543 37.0 28.0 38.0 11.0 38.0 36-37 31.766096202946997 37.0 27.0 38.0 11.0 38.0 38-39 31.74854649672385 37.0 27.0 38.0 11.0 38.0 40-41 31.822964839063605 37.0 27.5 38.0 11.0 38.0 42-43 31.908529270018356 37.0 29.0 38.0 11.0 38.0 44-45 32.015227177178716 38.0 31.0 38.0 11.0 38.0 46-47 31.89540928806538 37.5 29.0 38.0 11.0 38.0 48-49 31.807312121243193 37.0 27.0 38.0 11.0 38.0 50-51 31.7381130605088 37.0 27.0 38.0 11.0 38.0 52-53 31.831075746234223 37.0 27.0 38.0 11.0 38.0 54-55 31.788547317043157 37.0 27.0 38.0 11.0 38.0 56-57 31.753468412579597 37.0 27.0 38.0 11.0 38.0 58-59 31.77222808978395 37.0 27.0 38.0 11.0 38.0 60-61 31.844954523548292 37.0 28.0 38.0 11.0 38.0 62-63 31.667950124585992 37.0 27.0 38.0 11.0 38.0 64-65 31.62052028752192 37.0 27.0 38.0 11.0 38.0 66-67 31.577930334382657 37.0 27.0 38.0 11.0 38.0 68-69 31.49423212985655 37.0 27.0 38.0 11.0 38.0 70-71 31.434579535084033 37.0 27.0 38.0 11.0 38.0 72-73 31.326087179434595 37.0 27.0 38.0 11.0 38.0 74-75 31.31582293407709 37.0 26.0 38.0 11.0 38.0 76 29.837515252812157 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5140712121025182 1101 2 0.13896181905814942 1101 3 -0.19136691928184746 1101 4 0.8008000229819707 1101 5 0.12252773222841284 1101 6 -0.26578616436957603 1101 7 -0.07258778383081932 1101 8 -0.7461994072443403 1101 9 -0.25603475793212027 1101 10-11 -0.5044621318881823 1101 12-13 -0.9314478621990041 1101 14-15 -0.521684527138035 1101 16-17 -0.5178894349632692 1101 18-19 -0.2937667040398182 1101 20-21 -0.4772415133974732 1101 22-23 -0.2117860565252947 1101 24-25 -0.2760672455750708 1101 26-27 -0.38451014711895226 1101 28-29 -0.7454822477652776 1101 30-31 -0.4332035223166386 1101 32-33 -0.6830165028457955 1101 34-35 -0.2440007653708598 1101 36-37 -0.483521270128616 1101 38-39 -0.4465535938345404 1101 40-41 -0.5247877153099161 1101 42-43 -0.5553925204048227 1101 44-45 -0.37769775746052403 1101 46-47 -0.642263102820511 1101 48-49 -0.061812491303776085 1101 50-51 -0.2491290530991499 1101 52-53 -0.9755238263251833 1101 54-55 -0.2596603623321343 1101 56-57 -0.1772463280068557 1101 58-59 -0.6723843127161011 1101 60-61 -0.238010717802716 1101 62-63 -0.8881877699887717 1101 64-65 -0.2916878919156396 1101 66-67 -0.47888754847366144 1101 68-69 -0.46931385628127487 1101 70-71 -0.013834230970672934 1101 72-73 -0.11503770370145361 1101 74-75 -0.5517472821350218 1101 76 -1.0211615125053193 1102 1 -0.9214621586045766 1102 2 -1.003392090406873 1102 3 -0.8414903760719703 1102 4 -0.3781794008863386 1102 5 -1.040813831557596 1102 6 -0.7021647651926237 1102 7 -0.7396659731312312 1102 8 -0.9506603125941346 1102 9 -0.8310471036111338 1102 10-11 -0.870939498143322 1102 12-13 -1.0510198786599076 1102 14-15 -0.4458902884549083 1102 16-17 -1.0039964308480442 1102 18-19 -1.178597979759978 1102 20-21 -1.174360854961261 1102 22-23 -1.0436214474717964 1102 24-25 -1.5435240356985247 1102 26-27 -1.3773167314810912 1102 28-29 -1.3243464452961433 1102 30-31 -1.7260347980367996 1102 32-33 -1.0201852271256335 1102 34-35 -0.9723793661939055 1102 36-37 -0.6643525458487787 1102 38-39 -0.8658210835464715 1102 40-41 -0.9818412132522987 1102 42-43 -0.8563390224624321 1102 44-45 -0.8969323253617603 1102 46-47 -1.8205347077587852 1102 48-49 -1.8338701044724992 1102 50-51 -1.341664032523024 1102 52-53 -0.9556143613046082 1102 54-55 -1.6045739425790444 1102 56-57 -1.4332792498175557 1102 58-59 -0.9313225843210375 1102 60-61 -1.026142405045519 1102 62-63 -1.0103112267788958 1102 64-65 -0.7535315133148153 1102 66-67 -1.1383278776917685 1102 68-69 -1.1150422513430058 1102 70-71 -0.7198836136867257 1102 72-73 -1.2716385267467203 1102 74-75 -0.7043069529169159 1102 76 -0.6551697429579946 1103 1 0.12288401177807984 1103 2 0.8061558489088938 1103 3 0.7247524837032273 1103 4 0.4739642020864494 1103 5 0.6307665381985608 1103 6 -0.5495175076531602 1103 7 0.5221883355721637 1103 8 0.6366961151437174 1103 9 0.49721897341116517 1103 10-11 0.9190304054252536 1103 12-13 0.7836416900398042 1103 14-15 0.6017035325634552 1103 16-17 1.3596329530964333 1103 18-19 0.5347258332736118 1103 20-21 0.3067137104831197 1103 22-23 0.7007661822806774 1103 24-25 0.47545514248463405 1103 26-27 0.4611913454183636 1103 28-29 0.8912192447720351 1103 30-31 0.3902740896236594 1103 32-33 -0.002165756577138467 1103 34-35 0.5235514734351057 1103 36-37 0.47061305822958843 1103 38-39 0.34922252556844313 1103 40-41 0.7447495981229189 1103 42-43 0.5103537482519016 1103 44-45 0.7207201529872336 1103 46-47 0.6662443598660595 1103 48-49 0.9219188519798074 1103 50-51 0.6220052752590526 1103 52-53 0.29681945725690895 1103 54-55 0.1572650108021918 1103 56-57 1.014858149605086 1103 58-59 0.4665261350450933 1103 60-61 1.1078251030928001 1103 62-63 0.7201107374739131 1103 64-65 1.0044762871888402 1103 66-67 0.23587364555618962 1103 68-69 0.14444733774857355 1103 70-71 0.9532553212681307 1103 72-73 1.386813042567205 1103 74-75 1.2199989865216914 1103 76 1.2838235621215475 1104 1 -0.3013996792558018 1104 2 0.11248006723333148 1104 3 -0.10976107986578754 1104 4 0.1896467383104401 1104 5 -1.027311683829982 1104 6 0.04180507650633558 1104 7 -0.6339016524439565 1104 8 -0.5254402831567475 1104 9 -0.5498449769102223 1104 10-11 -0.01421395670569936 1104 12-13 0.1385959334214384 1104 14-15 -0.5420348921015403 1104 16-17 0.11374560153307911 1104 18-19 0.07502161712806554 1104 20-21 -0.14220501704710742 1104 22-23 0.31468109675938294 1104 24-25 0.26788895880449104 1104 26-27 0.08579642222411366 1104 28-29 -0.2715844375462986 1104 30-31 -0.03445899676918884 1104 32-33 0.043713424161506254 1104 34-35 0.27341529302330514 1104 36-37 -0.35781324093153444 1104 38-39 -0.7311375354403786 1104 40-41 -0.21037895618582425 1104 42-43 -0.012837775879273039 1104 44-45 0.13751392137158547 1104 46-47 -0.287372591871609 1104 48-49 -0.6602796445884458 1104 50-51 -0.1872604399604647 1104 52-53 -0.29127565114270126 1104 54-55 -0.3777333550328663 1104 56-57 0.03192155520482132 1104 58-59 -0.326384312716101 1104 60-61 -0.01387933094140692 1104 62-63 0.015213689865245072 1104 64-65 0.1809252467704887 1104 66-67 0.3784409186796225 1104 68-69 0.4850073116019331 1104 70-71 -0.10054955943783028 1104 72-73 0.11608638388978676 1104 74-75 0.10739140399636327 1104 76 -0.03704472418414895 1105 1 1.833776116141756 1105 2 1.5430534221115835 1105 3 0.8951979662143366 1105 4 1.1682504046613609 1105 5 1.3266193352818476 1105 6 2.0496336829586674 1105 7 1.5289389337264332 1105 8 0.39786166145794866 1105 9 1.1113240206938357 1105 10-11 1.3511256543713586 1105 12-13 0.35326969505290506 1105 14-15 0.7921017324039497 1105 16-17 0.7389197253420683 1105 18-19 1.494492837944918 1105 20-21 0.9593997079765693 1105 22-23 1.4327788289708927 1105 24-25 1.0112304643485999 1105 26-27 0.9740089368505167 1105 28-29 0.7007925613950228 1105 30-31 0.9298804471490101 1105 32-33 0.8296247185282475 1105 34-35 1.1935412193619612 1105 36-37 0.8675168978103187 1105 38-39 1.0573090015853097 1105 40-41 0.5985863304916101 1105 42-43 1.2521647315035764 1105 44-45 0.9260579677303085 1105 46-47 0.8009430040497172 1105 48-49 0.9067371270168358 1105 50-51 1.3309320156031355 1105 52-53 0.9881860973389394 1105 54-55 1.0335610117136653 1105 56-57 0.8462498551992503 1105 58-59 0.8434630155281724 1105 60-61 0.6873938623499498 1105 62-63 0.926758794896724 1105 64-65 0.8542968409087912 1105 66-67 1.1154025278622157 1105 68-69 1.1852357620393335 1105 70-71 0.7366237842964978 1105 72-73 0.7729928306496916 1105 74-75 0.48852752702528335 1105 76 0.6232048997084227 1106 1 -0.6845842232549337 1106 2 -0.1641459876333009 1106 3 -0.10341152894727301 1106 4 -0.16662005136747027 1106 5 -0.8285354647976106 1106 6 -0.5011764989420691 1106 7 -0.5829967057639038 1106 8 -0.38401353363839164 1106 9 -0.5795440516124195 1106 10-11 -0.8140606448993353 1106 12-13 0.4501580857563887 1106 14-15 -0.07821240817892772 1106 16-17 -0.2189749368220042 1106 18-19 -0.8623986743000387 1106 20-21 -0.5857916992710805 1106 22-23 -0.35114665132083545 1106 24-25 -0.2248999593297185 1106 26-27 -0.5288075448884655 1106 28-29 -0.2916235117058008 1106 30-31 -0.4284228531716572 1106 32-33 -0.19512059206512689 1106 34-35 -0.21748775421844257 1106 36-37 0.28753449195316705 1106 38-39 -0.5064271997824932 1106 40-41 0.48594834416963906 1106 42-43 -0.2776601783973831 1106 44-45 -0.07004719983970631 1106 46-47 -0.22984674594318832 1106 48-49 -0.23515821621083788 1106 50-51 -0.4277089787497097 1106 52-53 -0.17383609398317645 1106 54-55 -0.6643518121462577 1106 56-57 -0.7204730938061132 1106 58-59 -0.71114267702837 1106 60-61 -0.3998248441967718 1106 62-63 -0.6830130487991823 1106 64-65 -0.4516284123617389 1106 66-67 -1.023690522451357 1106 68-69 -0.7298937819318354 1106 70-71 -0.45736582948368465 1106 72-73 -0.28337227619216065 1106 74-75 -0.7241487691238682 1106 76 -0.6238083526540166 1107 1 0.04520291917161501 1107 2 -0.4135323894360603 1107 3 0.1802933123783852 1107 4 -0.6887598225778717 1107 5 -0.257379603678924 1107 6 -0.45698307556648743 1107 7 -0.8227808340238703 1107 8 -0.3125777856227181 1107 9 -0.4724980783954429 1107 10-11 -0.6415354909615374 1107 12-13 -0.01611195486309569 1107 14-15 0.08655485510134753 1107 16-17 -0.07932572839956364 1107 18-19 0.20823329596018425 1107 20-21 0.05094381478785337 1107 22-23 0.07598228324304657 1107 24-25 0.05869723318940956 1107 26-27 0.06847826986946615 1107 28-29 0.13508918080615118 1107 30-31 -0.32762051073362386 1107 32-33 0.11421901838972204 1107 34-35 -0.22526717463726698 1107 36-37 -0.2775212701286165 1107 38-39 0.3840796107986648 1107 40-41 0.13686479434259624 1107 42-43 -0.3399098949221937 1107 44-45 0.09889683713407038 1107 46-47 -0.07338279394020475 1107 48-49 -0.08663874613003131 1107 50-51 0.26817596620587025 1107 52-53 0.29665377985242714 1107 54-55 -0.2765792812510526 1107 56-57 0.06982703106649879 1107 58-59 0.2527662664344774 1107 60-61 -0.23572500351700398 1107 62-63 -0.1120796618806672 1107 64-65 -0.24174194596969656 1107 66-67 0.06562210403599167 1107 68-69 -0.15369995666738134 1107 70-71 -0.644760871897315 1107 72-73 0.435147624483875 1107 74-75 0.1438666174788743 1107 76 0.13083848749468174 1108 1 -0.18568807034006696 1108 2 -0.10158990507978416 1108 3 -0.39236308786422214 1108 4 0.0840030881160736 1108 5 -0.044077631756263 1108 6 -0.764176968967277 1108 7 0.10097543455998448 1108 8 -0.6238545796581327 1108 9 -0.7037588958631567 1108 10-11 -0.03916711273109286 1108 12-13 -0.9349421150725625 1108 14-15 -0.6405121133449327 1108 16-17 -0.21883962653415168 1108 18-19 -0.12126861974862635 1108 20-21 -0.8078928543936428 1108 22-23 -0.6239393132302773 1108 24-25 -0.6920978969160672 1108 26-27 -0.09180516627603907 1108 28-29 -0.2819879948917148 1108 30-31 -0.3736862809373278 1108 32-33 -0.2690854683630377 1108 34-35 0.05564674420768512 1108 36-37 -0.04955192146961451 1108 38-39 -0.5466072336812822 1108 40-41 -0.47623599117198623 1108 42-43 -0.6799519073779976 1108 44-45 -0.5953146156980722 1108 46-47 -0.029772681364573117 1108 48-49 -0.32414965605473256 1108 50-51 -0.12660798030221798 1108 52-53 -0.3650793818807401 1108 54-55 0.015902856058673365 1108 56-57 -0.011468550229079 1108 58-59 -0.09075212880805594 1108 60-61 0.20899311361490192 1108 62-63 0.16870111890011685 1108 64-65 -0.13596375398460836 1108 66-67 -0.1884201155234706 1108 68-69 -0.3023560018751468 1108 70-71 -0.055558368901706956 1108 72-73 -0.46775034737961363 1108 74-75 -0.13697333577487214 1108 76 -0.6239507845359711 1109 1 0.29271666668536156 1109 2 0.6699618190581482 1109 3 0.28954217162724305 1109 4 0.06967881086076133 1109 5 0.1770125807132601 1109 6 1.0564865629031495 1109 7 1.3069576707146346 1109 8 0.3044369563920277 1109 9 0.6169046360072699 1109 10-11 0.1948105953845456 1109 12-13 0.12200668325554531 1109 14-15 0.6740124425589329 1109 16-17 0.5796408680670346 1109 18-19 0.5434605686874576 1109 20-21 0.2909403047843462 1109 22-23 0.8460624283231901 1109 24-25 0.5079630574552354 1109 26-27 0.48103530742650236 1109 28-29 0.5303813885983608 1109 30-31 0.41269041707730025 1109 32-33 0.2596501638208757 1109 34-35 0.7124083255382345 1109 36-37 0.7319484268410861 1109 38-39 0.6851282243472809 1109 40-41 0.23137895135674924 1109 42-43 0.5705923280800249 1109 44-45 0.6444537576909894 1109 46-47 0.5211459880885805 1109 48-49 0.48068750869622434 1109 50-51 0.8019012499311522 1109 52-53 0.4025973857960281 1109 54-55 0.38286994069817126 1109 56-57 0.6141627629022359 1109 58-59 0.7531611418293522 1109 60-61 0.8227165549245541 1109 62-63 0.9445849572839542 1109 64-65 0.835039380811633 1109 66-67 1.075991239405127 1109 68-69 0.7478225073550817 1109 70-71 0.9582869811505361 1109 72-73 0.27741684175309445 1109 74-75 0.4828890815013409 1109 76 0.5020506087068028 1110 1 -1.0377440745560484 1110 2 -0.17901587610913694 1110 3 0.46164423313451053 1110 4 -1.0327910550849424 1110 5 -0.6686841636823679 1110 6 -0.5234813316558231 1110 7 -0.03281083215795633 1110 8 -0.13122542954917193 1110 9 -0.8211797393447604 1110 10-11 -0.8047669646019351 1110 12-13 -0.787760129856995 1110 14-15 -0.5447551591082984 1110 16-17 -0.9754805130301847 1110 18-19 -0.42745443638182223 1110 20-21 -1.135977572877028 1110 22-23 -0.5593250899825435 1110 24-25 -0.6115170597014625 1110 26-27 -0.8410752028810329 1110 28-29 -0.8399506492522697 1110 30-31 -0.9655975743612473 1110 32-33 -0.7861986589796253 1110 34-35 -1.1970416575641671 1110 36-37 -0.6641383705003641 1110 38-39 -0.946947645879149 1110 40-41 -0.0010405034140070768 1110 42-43 -0.7144631523750817 1110 44-45 -0.2447683894307886 1110 46-47 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2116 28-29 -0.6843552636382952 2116 30-31 -0.17253685564997312 2116 32-33 -0.34947682030611205 2116 34-35 -1.0342547336248273 2116 36-37 -0.6128387304460787 2116 38-39 -0.27491867319961827 2116 40-41 -0.543422635944836 2116 42-43 -0.35428140929370855 2116 44-45 -0.6107771225398935 2116 46-47 -0.46361230916971863 2116 48-49 -0.7841934436847282 2116 50-51 -0.4376369896070891 2116 52-53 -0.5734127152140722 2116 54-55 -0.32591433058610164 2116 56-57 -0.41226220102273103 2116 58-59 -0.5624954238272153 2116 60-61 -0.7714392892312887 2116 62-63 -0.7281242779252786 2116 64-65 -0.9257831300108776 2116 66-67 -0.9103002468863579 2116 68-69 -0.5314884594558826 2116 70-71 -0.6665326436690862 2116 72-73 -0.17081548147923087 2116 74-75 -0.136699663087402 2116 76 -0.48820913155293866 2117 1 0.13570298144587056 2117 2 0.18609085131621228 2117 3 0.08169759684718514 2117 4 0.7293806681432642 2117 5 0.7035277322284124 2117 6 1.0066331904691346 2117 7 1.0741864097175657 2117 8 0.18273607662662883 2117 9 -0.24880895148050897 2117 10-11 -0.11713955124302089 2117 12-13 -0.2843349589731936 2117 14-15 0.6639928922168039 2117 16-17 -0.10242169302778592 2117 18-19 -0.0820892846849759 2117 20-21 0.08969397047349403 2117 22-23 -0.07426992749303807 2117 24-25 -0.02692208428474885 2117 26-27 -0.050123050344758724 2117 28-29 0.18030807481536826 2117 30-31 0.047893251876910625 2117 32-33 0.07685446489614023 2117 34-35 -0.1930168944031152 2117 36-37 -0.20695675399958446 2117 38-39 -0.12498907770550716 2117 40-41 -0.0502070701486268 2117 42-43 0.19791393120808465 2117 44-45 0.25228611350721764 2117 46-47 0.0028175423407752476 2117 48-49 0.10770363772848057 2117 50-51 0.5270967533524633 2117 52-53 0.33362133496513735 2117 54-55 0.22096866992593078 2117 56-57 -0.589649553813306 2117 58-59 -0.2941262481999729 2117 60-61 -0.16170426618981537 2117 62-63 0.12860255259187525 2117 64-65 -0.16752660159306032 2117 66-67 -0.3933391613768862 2117 68-69 -0.17226546918450225 2117 70-71 -0.06835036000293115 2117 72-73 -1.391021574669196 2117 74-75 -0.5178440563285704 2117 76 -0.7280324802472542 2118 1 0.49019997433816087 2118 2 0.4390296156683178 2118 3 0.7494805383452707 2118 4 -0.3474372651536193 2118 5 0.14208705426231205 2118 6 -0.10087091013228999 2118 7 0.6596156059996865 2118 8 0.510715846992948 2118 9 0.1869482929153392 2118 10-11 -0.1172078946000461 2118 12-13 0.5596029852586248 2118 14-15 0.9755019135399294 2118 16-17 0.5721614124943564 2118 18-19 0.08958922816357529 2118 20-21 0.5214873001618479 2118 22-23 0.8357902146611487 2118 24-25 0.6223056357808616 2118 26-27 0.2553034122030837 2118 28-29 0.48829741325166864 2118 30-31 0.20528800310709272 2118 32-33 0.47222078528980305 2118 34-35 0.55264330242575 2118 36-37 0.2245973739391829 2118 38-39 0.8870735248095301 2118 40-41 0.5551783863850055 2118 42-43 0.5409634117985718 2118 44-45 0.2225225815225187 2118 46-47 -0.1195512384137345 2118 48-49 0.16606886462842496 2118 50-51 0.43319298079915214 2118 52-53 0.7942897329968517 2118 54-55 0.02369556987125776 2118 56-57 0.42261807877280333 2118 58-59 0.4998868737245772 2118 60-61 0.22795538389220127 2118 62-63 0.32854104357054936 2118 64-65 0.29748159960978526 2118 66-67 0.44912940067887774 2118 68-69 0.28355055049838285 2118 70-71 0.08091153174119015 2118 72-73 0.4056911098578695 2118 74-75 0.265439158542943 2118 76 0.5799910298675641 2119 1 0.3741798339225859 2119 2 -0.7991804403560785 2119 3 -0.8296095971061135 2119 4 -0.7385974665577777 2119 5 0.4007034644459857 2119 6 -0.07716691750765392 2119 7 0.05586409901436795 2119 8 -0.7793876917631692 2119 9 -0.6373904064676843 2119 10-11 0.05435795179382552 2119 12-13 -0.14520518437473484 2119 14-15 -0.203634317933016 2119 16-17 0.0551147491371502 2119 18-19 -0.2541098002741258 2119 20-21 -0.3316348188368039 2119 22-23 -0.6006061402073009 2119 24-25 -0.37822624139096916 2119 26-27 -0.41545575381351085 2119 28-29 0.378065869389534 2119 30-31 -0.3559399239902774 2119 32-33 -0.08693282083742915 2119 34-35 -0.3515656189273457 2119 36-37 -0.3632953287060232 2119 38-39 -0.47327325910650586 2119 40-41 -0.2347124015023816 2119 42-43 -0.27710800157636584 2119 44-45 -0.7007186779626196 2119 46-47 -0.5579953203937329 2119 48-49 -0.0012685582493823233 2119 50-51 -0.6988277978690256 2119 52-53 -0.30533972590677294 2119 54-55 -0.11916663848275988 2119 56-57 -0.4796228970445142 2119 58-59 0.39118890904121884 2119 60-61 -0.2993831027399594 2119 62-63 -0.1239534603653425 2119 64-65 -0.3634201094888603 2119 66-67 -0.19149005893391546 2119 68-69 -0.31691218264111143 2119 70-71 -0.1963028502175348 2119 72-73 0.3914811247504346 2119 74-75 -0.4000987465701691 2119 76 0.4195414163649751 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 16.0 13 235.0 14 1058.0 15 2145.0 16 2928.0 17 3361.0 18 3552.0 19 3119.0 20 2403.0 21 1683.0 22 1246.0 23 1003.0 24 895.0 25 860.0 26 870.0 27 1094.0 28 1161.0 29 1372.0 30 1652.0 31 2052.0 32 2628.0 33 3144.0 34 4367.0 35 6441.0 36 11873.0 37 36364.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.91034935348584 18.511530613291225 11.249653927791394 29.328466105431538 2 19.807635121971227 18.222368056766097 30.160064805225435 31.80993201603724 3 19.04576356346708 19.172913056407207 29.35205028557366 32.42927309455205 4 15.70603857551552 20.991971124760315 26.465551716005454 36.83643858371871 5 16.878069788665236 29.23823098140951 31.625360171446737 22.258339058478512 6 29.749905150579863 27.718589460947673 16.32743045230356 26.204074936168904 7 28.082606154445617 26.385570583349573 17.791700419388246 27.740122842816568 8 23.774904381530508 28.04979338207397 17.516893450775715 30.6584087856198 9 23.528808588743168 16.81039344564872 18.077786778503533 41.58301118710458 10-11 26.83623350389139 21.21345733826892 22.866913446058877 29.083395711780813 12-13 24.98590076187156 18.43462567804518 23.345774842857583 33.23369871722568 14-15 24.954113388636525 19.784050941829108 21.314459153225393 33.94737651630898 16-17 26.752663474257353 21.207817643017545 21.08887134317033 30.950647539554772 18-19 25.740594526419407 20.045015022097353 21.98660828727582 32.22778216420742 20-21 25.155091619412858 20.59052736277596 22.92074690073111 31.333634117080074 22-23 27.308429806302104 20.39518882725101 21.212944638700616 31.083436727746278 24-25 25.82621535432667 22.153748346543892 20.52643991673759 31.493596382391846 26-27 24.711093793259025 22.49007926335326 22.41419972724383 30.384627216143883 28-29 25.689324569588713 21.508259590045427 23.68877085405494 29.11364498631092 30-31 25.4529700685992 20.420823805666355 23.895901479650956 30.23030464608349 32-33 24.37540054859135 22.075931195365172 23.813991642954193 29.734676613089288 34-35 24.55677122319863 22.115808578489176 23.29911918214165 30.028301016170545 36-37 24.905406929647363 20.5566891912677 24.130717881935542 30.407185997149387 38-39 23.636988197655935 20.282394922223475 26.815725521159113 29.264891358961474 40-41 23.477025932344166 19.9086369369277 26.84700019482584 29.7673369359023 42-43 21.80254914225362 20.008613352747556 26.860330383601816 31.328507121397003 44-45 20.873537524481403 19.615372783856117 28.94650492704285 30.564584764619628 46-47 20.966336146344965 21.043753781159317 28.721429816556093 29.26848025593962 48-49 20.895070906350295 21.727182305712496 27.702183074761848 29.67556371317535 50-51 20.86277083354696 22.885883330086237 26.04872696697189 30.20261886939491 52-53 19.60306799421675 23.077120269064732 26.53374075859028 30.786070978128237 54-55 19.6584395475939 21.846128605559713 26.91672733611558 31.578704510730805 56-57 20.4095444151636 22.28602483516709 26.166647867682496 31.137782881986816 58-59 20.66025450406571 22.01583216266932 25.44989387118936 31.874019462075616 60-61 20.04552772166566 21.26934159121438 26.263548086092513 32.421582601027445 62-63 20.70537206607672 21.64617577392 25.543717892189534 32.10473426781375 64-65 20.97710283727941 22.093762497051976 24.54856803010572 32.38056663556289 66-67 20.756642022907418 23.35602883422372 22.969966059288577 32.917363083580284 68-69 20.723316550967464 24.71211919239564 22.56236990248454 32.00219435415235 70-71 20.422874603939583 25.21661556760969 23.952811131733025 30.4076986967177 72-73 20.749976928519427 24.286065851132555 25.426822390615545 29.537134829732477 74-75 20.232150364529396 26.247141699906688 24.223003804230796 29.29770413133312 76 19.370815089773693 25.841083641807572 23.110445741004686 31.677655527414046 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.5 15 3.5 16 6.0 17 6.0 18 6.0 19 9.5 20 20.5 21 28.0 22 34.5 23 47.0 24 73.5 25 94.0 26 145.0 27 211.0 28 272.5 29 319.0 30 411.0 31 580.0 32 798.5 33 940.0 34 1055.5 35 1298.0 36 1581.5 37 1738.0 38 2015.5 39 2535.0 40 2994.0 41 3457.0 42 3703.0 43 3778.5 44 4089.5 45 4260.0 46 4195.0 47 4246.5 48 4136.5 49 3809.0 50 3643.0 51 3458.5 52 2967.0 53 2642.0 54 2624.0 55 2618.0 56 2606.5 57 2611.0 58 2621.0 59 2710.5 60 2873.5 61 3032.5 62 3118.0 63 3191.5 64 3283.5 65 3239.5 66 3257.0 67 3337.0 68 3315.0 69 3120.5 70 2838.5 71 2729.0 72 2521.0 73 2039.5 74 1445.0 75 1124.0 76 908.5 77 522.0 78 227.0 79 103.0 80 76.0 81 34.0 82 17.5 83 16.0 84 9.5 85 6.5 86 7.5 87 5.0 88 7.5 89 8.0 90 5.0 91 4.0 92 4.0 93 3.0 94 4.0 95 4.0 96 2.0 97 2.5 98 4.0 99 13.5 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 5.126995683069635E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 97523.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.9345897890754 #Duplication Level Percentage of deduplicated Percentage of total 1 79.45675402092003 57.951457604872694 2 12.591384546170284 18.36694933502866 3 4.463783601394669 9.766926776247654 4 1.6364863344955571 4.7742583800744445 5 0.7198290406028568 2.625021789731653 6 0.39365650657968737 1.7226705495113972 7 0.2558767292767967 1.306358500046143 8 0.16870993139129456 0.9843831711493698 9 0.09560229445506692 0.6275442716077233 >10 0.2179169947137555 1.8744296217302585 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001025399136613927 0.0 2 0.0 0.0 0.0 0.01025399136613927 0.0 3 0.0 0.0 0.0 0.011279390502753197 0.0 4 0.0 0.0 0.0 0.02358418014212032 0.0 5 0.0 0.0 0.0 0.02973657496180388 0.0 6 0.0 0.0 0.0 0.02973657496180388 0.0 7 0.0 0.0 0.0 0.03076197409841781 0.0 8 0.0 0.0 0.0 0.03076197409841781 0.0 9 0.0 0.0 0.0 0.03281277237164566 0.0 10 0.0 0.0 0.0 0.04511756201101279 0.0 11 0.0 0.0 0.0 0.04614296114762671 0.0 12 0.0 0.0 0.0 0.04819375942085457 0.0 13 0.0 0.0 0.0 0.05126995683069635 0.0 14 0.0 0.0 0.0 0.0533207551039242 0.0 15 0.0 0.0 0.0 0.05742235165037991 0.0 16 0.0 0.0 0.0 0.0646001456066774 0.0 17 0.0 0.0 0.0 0.06972714128974704 0.0 18 0.0 0.0 0.0 0.07382873783620274 0.0 19 0.0 0.0 0.0 0.0758795361094306 0.0 20 0.0 0.0 0.0 0.08305733006572809 0.0 21 0.0 0.0 0.0 0.08920972488541165 0.0 22 0.0 0.0 0.0 0.09843831711493699 0.0 23 0.0 0.0 0.0 0.10766690934446234 0.0 24 0.0 0.0 0.0 0.11484470330075983 0.0 25 0.0 0.0 0.0 0.140479681716108 0.0 26 0.0 0.0 0.0 0.15893686617515868 0.0 27 0.0 0.0 0.0 0.2594259815633235 0.0 28 0.0 0.0 0.0 0.4040072598258872 0.0 29 0.0 0.0 0.0 0.5588425294545902 0.0 30 0.0 0.0 0.0 0.701373009443926 0.0 31 0.0 0.0 0.0 0.8961988454005722 0.0 32 0.0 0.0 0.0 1.1033294709965855 0.0 33 0.0 0.0 0.0 1.3596792551500672 0.0 34 0.0 0.0 0.0 1.7072895624621884 0.0 35 0.0 0.0 0.0 2.2087097402663987 0.0 36 0.0 0.0 0.0 2.9100827497103245 0.0 37 0.0 0.0 0.0 3.920100899275043 0.0 38 0.0 0.0 0.0 4.836807727407893 0.0 39 0.0 0.0 0.0 5.721727182305712 0.0 40 0.0 0.0 0.0 6.596392645837392 0.0 41 0.0 0.0 0.0 7.527455061882838 0.0 42 0.0 0.0 0.0 8.507736636485753 0.0 43 0.0 0.0 0.0 9.458281636126863 0.0 44 0.0 0.0 0.0 10.395496446991991 0.0 45 0.0 0.0 0.0 11.253755524337848 0.0 46 0.0 0.0 0.0 12.265824472175794 0.0 47 0.0 0.0 0.0 13.09742317196969 0.0 48 0.0 0.0 0.0 13.948504455359249 0.0 49 0.0 0.0 0.0 14.689868031131118 0.0 50 0.0 0.0 0.0 15.339971083744347 0.0 51 0.0 0.0 0.0 16.057750479374096 0.0 52 0.0 0.0 0.0 16.84935861284005 0.0 53 0.0 0.0 0.0 17.731201870328025 0.0 54 0.0 0.0 0.0 18.491022630558945 0.0 55 0.0 0.0 0.0 19.23648780287727 0.0 56 0.0 0.0 0.0 19.898895645129866 0.0 57 0.0 0.0 0.0 20.600268654573792 0.0 58 0.0 0.0 0.0 21.172441372804364 0.0 59 0.0 0.0 0.0 21.81639203059791 0.0 60 0.0 0.0 0.0 22.42752991601981 0.0 61 0.0 0.0 0.0 23.019185217846047 0.0 62 0.0 0.0 0.0 23.59956112916953 0.0 63 0.0 0.0 0.0 24.32861991530203 0.0 64 0.0 0.0 0.0 24.979748367051876 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGTT 15 0.0022187857 70.00001 19 GTTTACC 20 0.006933065 52.500004 36 TGGAATA 20 0.006933065 52.500004 36 ATTCACG 20 0.006933065 52.500004 27 GCCTAAA 20 0.006933065 52.500004 11 TTTTGGT 20 0.006933065 52.500004 23 CGCTTTG 20 0.006933065 52.500004 53 CAAAGAG 20 0.006933065 52.500004 56 AACCGTT 30 5.852823E-4 46.666668 70 TTGTGCC 30 5.852823E-4 46.666668 38 CATAAGG 35 0.0012501258 40.0 29 CGACGAT 75 3.4855475E-8 37.333332 22 CACGACG 40 0.0024086267 35.000004 12 TATAGTG 40 0.0024086267 35.000004 5 TCATAAC 40 0.0024086267 35.000004 2 ATTGCCA 40 0.0024086267 35.000004 46 GGGTGTG 45 0.004289335 31.111113 4 TGGCTAT 50 0.0071785557 27.999998 42 GACGATG 70 0.0012185192 25.0 23 CTCATAA 90 1.9113172E-4 23.333334 1 >>END_MODULE