FastQCFastQC Report
Wed 25 May 2016
SRR1780264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97523
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT1017410.432410815910092No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC29443.018775058191401No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG28082.8793207756119066No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG22912.3491894219825067RNA PCR Primer, Index 34 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6310.6470268552033879No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT3420.350686504721963RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT2930.3004419470278806No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC2050.21020682300585503No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1720.17636865149759545No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT1510.15483526962870298No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1390.14253047998933585No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1040.10664151020784841No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAT150.002218785770.000011
TAACACC150.002218785770.0000141
TCTTTAT650.059.2307661
GAAGAGT200.00693306552.50000436
TAGAATA200.00693306552.5000042
GTCAGAT200.00693306552.5000043
AGACAGG200.00693306552.50000419
CGTCAGA200.00693306552.5000042
TAGCTAC200.00693306552.50000453
ACGCGCC200.00693306552.50000411
CTTAATA451.6698523E-646.6666681
CTTTATA851.8189894E-1245.2941132
CCTTATA350.001250125840.02
TAATACA556.636388E-638.1818163
TTAATAC556.636388E-638.1818162
TAGCACC1400.037.533
TTTATAC1051.2732926E-1136.6666683
CTTGAAA21900.035.1598257
TGAAAAA21950.035.07972759
CCTCGTA1500.035.038
Produced by FastQC (version 0.11.5)