FastQCFastQC Report
Wed 25 May 2016
SRR1780263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449055
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTTC250.00235783235.17986742
CTTTATA250.00235783235.1798672
AATAGAG353.227577E-431.4070975
CTTGTCA300.005726023629.34923739
ATGTAGT300.005757292729.3165552
CGAAACT609.744728E-625.66341625
GAAACTC551.5928094E-423.99696226
AAACTCG651.811872E-523.68930827
CCCTAAG500.00256983122.01192738
CTATACA654.9445644E-420.2960781
GTATAGA550.00449415419.988561
TACACCA809.019766E-519.2368475
AGACAGG951.6028776E-518.5136598
TAGATCT1801.4551915E-1118.34327539
CTGTGTA600.007381904418.34327334
ATTATGG600.00739140518.33918412
CTTAATA600.007429507618.3228472
CCTAGTC600.007429507618.3228472
TGCGTGT600.007429507618.32284733
CTAGTCC600.007434281518.3208073
Produced by FastQC (version 0.11.5)