FastQCFastQC Report
Wed 25 May 2016
SRR1780263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449055
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT225515.021879279820958No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC67531.5038246985335872No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG62771.3978243199608065No Hit
CACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCTG54011.202747993007538TruSeq Adapter, Index 2 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC13590.3026355346227077No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT6270.1396265490864148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG207.845971E-444.0061233
TCTTCTG6200.039.3838144
CTTTATA1750.038.9768522
GTCTTCT6500.037.56609343
TCTTTAT1950.035.0690571
CCGTCTT7050.034.94743741
TTTATAC2150.032.7487453
CGTCTTC7650.032.2064642
ATGCCGT31350.031.9973344
CCTTATA300.00566583329.412761
GCCGTCT8450.028.89699440
TAACCGG407.0286356E-427.49770422
TGACTAT407.0286356E-427.49770437
TGCCGTC9150.026.92671439
TATGCCG37850.026.5024143
CTTAATA959.295036E-1025.542661
TTAATAC959.567884E-1025.477232
CTACACT450.001395714524.4478474
CTTTACA551.5625138E-424.0649851
ACACATC45350.022.3159476
Produced by FastQC (version 0.11.5)