FastQCFastQC Report
Wed 25 May 2016
SRR1780262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91272
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT200.006931648652.4999966
TACTCTG200.006931648652.49999615
GTCTTGG200.006931648652.49999626
AAGGATT200.006931648652.49999620
ATGTACC305.850838E-446.66666411
CACATTT400.0024078234.9999967
TACCCGA450.004287906531.11110914
TGTATAT450.004287906531.11110937
ACCCGAT450.004287906531.11110915
TGTACCC450.004287906531.11110912
GTACCCG500.007176178527.99999813
TACCCGT500.007176178527.9999981
CGAAGAA657.9030154E-426.92307715
AGCCGCC901.9100086E-423.33333212
TGTAGAT850.00376447720.58823637
CCCGGAG900.00524045919.4444432
CCAGGCC1159.983697E-418.260873
ATTGAAC3900.017.05128370
AATTGAA5500.015.90909170
TGAACCG2653.3744072E-815.84905770
Produced by FastQC (version 0.11.5)