##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 91272 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.090334385134543 34.0 23.0 34.0 12.0 34.0 2 28.390514067841178 34.0 23.0 34.0 12.0 34.0 3 28.164957489701113 34.0 23.0 34.0 11.0 34.0 4 28.078096239810677 34.0 23.0 34.0 11.0 34.0 5 27.994302743448156 34.0 23.0 34.0 11.0 34.0 6 30.353591462880182 37.0 25.0 38.0 11.0 38.0 7 30.70148566920852 37.0 26.0 38.0 12.0 38.0 8 30.795358927162766 37.0 26.0 38.0 11.0 38.0 9 31.003834691909894 37.0 26.0 38.0 12.0 38.0 10-11 30.652993250942238 37.0 25.5 38.0 11.0 38.0 12-13 30.733954553422734 37.0 26.0 38.0 11.0 38.0 14-15 30.53783745288807 37.0 25.0 38.0 11.0 38.0 16-17 30.58191997545797 37.0 25.5 38.0 11.0 38.0 18-19 30.596349373301777 37.0 25.0 38.0 11.0 38.0 20-21 30.579980716977825 37.0 25.5 38.0 11.0 38.0 22-23 30.600934569199755 37.0 25.0 38.0 11.0 38.0 24-25 30.561327679901833 37.0 24.5 38.0 11.0 38.0 26-27 30.45845385222193 37.0 24.0 38.0 11.0 38.0 28-29 30.489432684722587 37.0 24.5 38.0 11.0 38.0 30-31 30.47501424314138 37.0 24.5 38.0 11.0 38.0 32-33 30.544937111052676 37.0 25.0 38.0 11.0 38.0 34-35 30.53635835743711 37.0 25.0 38.0 11.0 38.0 36-37 30.462359759838726 37.0 24.5 38.0 11.0 38.0 38-39 30.42499342624244 37.0 24.5 38.0 11.0 38.0 40-41 30.485910246296783 37.0 25.0 38.0 11.0 38.0 42-43 30.5662415636778 37.0 25.0 38.0 11.0 38.0 44-45 30.685089622228066 37.0 25.5 38.0 11.0 38.0 46-47 30.580358708037515 37.0 25.0 38.0 11.0 38.0 48-49 30.46726268735209 37.0 24.5 38.0 11.0 38.0 50-51 30.410060040319046 37.0 24.0 38.0 11.0 38.0 52-53 30.503763476202998 37.0 24.5 38.0 11.0 38.0 54-55 30.477041151722325 37.0 24.0 38.0 11.0 38.0 56-57 30.427414760276974 37.0 24.0 38.0 11.0 38.0 58-59 30.464090849329477 37.0 24.5 38.0 11.0 38.0 60-61 30.49962200894031 37.0 25.0 38.0 11.0 38.0 62-63 30.339145630642477 37.0 24.0 38.0 11.0 38.0 64-65 30.242352528705407 37.0 24.0 38.0 11.0 38.0 66-67 30.184004952230694 37.0 24.0 38.0 11.0 38.0 68-69 30.12934415812078 37.0 23.5 38.0 11.0 38.0 70-71 30.03972740818652 37.0 23.0 38.0 11.0 38.0 72-73 29.858812122008942 37.0 23.0 38.0 11.0 38.0 74-75 29.832106231922168 37.0 23.0 38.0 11.0 38.0 76 28.422221491804716 34.0 20.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04137811425168181 1101 2 -0.06365508097765016 1101 3 0.10709652000074499 1101 4 -0.6012239194443687 1101 5 -0.28989082352076423 1101 6 -0.18820417082644525 1101 7 -0.6882544775091652 1101 8 -0.6699293678269029 1101 9 -0.7292395722213136 1101 10-11 -0.30708032470096924 1101 12-13 0.3082347446809095 1101 14-15 -0.10976142800626576 1101 16-17 -0.2887431582866711 1101 18-19 -0.48996090269340087 1101 20-21 0.45118276097490906 1101 22-23 -0.08605229962491023 1101 24-25 -0.4290435113891249 1101 26-27 0.10681810420097548 1101 28-29 -0.09328417726027993 1101 30-31 0.23205295732053344 1101 32-33 -0.2563490896780358 1101 34-35 0.4892170233790374 1101 36-37 0.033544860197164184 1101 38-39 -0.5904468289118121 1101 40-41 0.018210576016947755 1101 42-43 -0.5060430149219286 1101 44-45 -5.647988568888707E-4 1101 46-47 0.10830870531888337 1101 48-49 -0.38224900560461705 1101 50-51 -0.3639007344661387 1101 52-53 0.03136215576072132 1101 54-55 -0.3184434473714539 1101 56-57 -0.018867105998616296 1101 58-59 0.04007154103035404 1101 60-61 -0.7044505410056949 1101 62-63 -0.28761580685443633 1101 64-65 0.31622189504102494 1101 66-67 0.0744518066456834 1101 68-69 -0.28110005845178065 1101 70-71 -0.005794531391671853 1101 72-73 -0.5718385425346604 1101 74-75 -0.8192372081327903 1101 76 -1.481328595935448 1102 1 -0.260632554202445 1102 2 -0.9277662116264977 1102 3 -0.37950449986913526 1102 4 0.2802601670687288 1102 5 -0.6813626012594689 1102 6 -0.33251575865834226 1102 7 -0.9533327261464066 1102 8 -0.07314723313374571 1102 9 -0.01299199001628537 1102 10-11 -0.5878346767319158 1102 12-13 -0.629904871988689 1102 14-15 -0.34246759997742515 1102 16-17 -0.03191178849592191 1102 18-19 -0.7606132677364847 1102 20-21 -0.37208082263683195 1102 22-23 -1.0550253961608682 1102 24-25 -1.3317074818480634 1102 26-27 -0.8829392371037557 1102 28-29 -0.21485090840148047 1102 30-31 -0.2545832304761966 1102 32-33 -0.021409754351854104 1102 34-35 -0.417684682265417 1102 36-37 -0.5347248778017821 1102 38-39 -0.3922316011994873 1102 40-41 -1.2405462377688785 1102 42-43 -0.2869116627151378 1102 44-45 -0.5984898911727008 1102 46-47 -0.8773427805260248 1102 48-49 -1.2357657982896875 1102 50-51 -0.7390018422558349 1102 52-53 -0.7752494144872166 1102 54-55 -1.3833458563744436 1102 56-57 -0.5978454777236024 1102 58-59 -0.3759562416318616 1102 60-61 -0.45995432155783433 1102 62-63 -0.6413172311554725 1102 64-65 -0.5180492496749913 1102 66-67 -0.5532868956578127 1102 68-69 -0.21902866756027706 1102 70-71 -0.5880962722040515 1102 72-73 -0.31253662588264675 1102 74-75 -0.1402148764992397 1102 76 -0.47793489099435504 1103 1 -1.0457903918938136 1103 2 -0.8125040520275277 1103 3 -0.4595410802782922 1103 4 -0.16453051170108424 1103 5 -0.24927345344751828 1103 6 -0.8365125070664199 1103 7 -0.6562523847292567 1103 8 -1.1167727581303595 1103 9 -0.7995046576764935 1103 10-11 -1.4797869867170164 1103 12-13 -1.3579058203696626 1103 14-15 -1.0938563006954851 1103 16-17 -0.31076966683219 1103 18-19 -0.8374321269696487 1103 20-21 -0.7862692794855022 1103 22-23 -1.1419208032872739 1103 24-25 -1.3300986212600705 1103 26-27 -0.5455188333644259 1103 28-29 -1.1045677489379955 1103 30-31 -1.5205194179846657 1103 32-33 -1.372158297909639 1103 34-35 -1.2585768377998967 1103 36-37 -0.7827265035977433 1103 38-39 -1.1181325631287642 1103 40-41 -0.8348762743492877 1103 42-43 -1.581705728559875 1103 44-45 -1.2881999575593674 1103 46-47 -0.8827621004013828 1103 48-49 -0.8667101147779697 1103 50-51 -0.8147029667647097 1103 52-53 -0.837080467190102 1103 54-55 -0.32275108601812263 1103 56-57 -0.8877282849312991 1103 58-59 -1.0264439804206411 1103 60-61 -1.0645133244029772 1103 62-63 -1.0018205504888975 1103 64-65 -1.284798335164762 1103 66-67 -0.9505569132706029 1103 68-69 -1.1922877110503158 1103 70-71 -0.3768444093128416 1103 72-73 -0.981229892377705 1103 74-75 -1.3643714948436383 1103 76 -1.7281719862389018 1104 1 1.6759176990633513 1104 2 0.7471204852543352 1104 3 0.8600649482780263 1104 4 0.6166038087642782 1104 5 -0.16003495529839284 1104 6 0.31294545168556454 1104 7 0.4678882815848624 1104 8 -0.14546128272052172 1104 9 1.0566561038253575 1104 10-11 0.894386730892716 1104 12-13 0.4350278126287499 1104 14-15 0.2723851059676541 1104 16-17 0.28008834480186806 1104 18-19 0.5408730025914288 1104 20-21 0.29450808698726405 1104 22-23 0.6159123537724831 1104 24-25 1.2415896389197272 1104 26-27 0.4517048578042733 1104 28-29 0.37477519130526105 1104 30-31 0.06733092574880928 1104 32-33 0.4191227703082383 1104 34-35 -0.17386636714944714 1104 36-37 0.5840767750907823 1104 38-39 0.16881878536409545 1104 40-41 0.6403022026470104 1104 42-43 0.6332929493882986 1104 44-45 0.708494912879555 1104 46-47 0.6631130979063933 1104 48-49 0.6075759772368379 1104 50-51 0.013364193467968022 1104 52-53 0.5644266718897519 1104 54-55 0.2834096754837283 1104 56-57 0.11508107519561861 1104 58-59 0.5226041422657666 1104 60-61 0.455218299076666 1104 62-63 0.6514713585539376 1104 64-65 0.2732397399277815 1104 66-67 0.6632386975173397 1104 68-69 0.6361099621384732 1104 70-71 0.5535826134264461 1104 72-73 0.9072880875271103 1104 74-75 0.30904865322616715 1104 76 0.880236263364484 1105 1 0.3573693119665755 1105 2 0.9930882271898192 1105 3 -0.32138666462519794 1105 4 0.6206360668287942 1105 5 1.3641585930887068 1105 6 1.4782680323307247 1105 7 1.4634610099406586E-4 1105 8 0.5198612979246384 1105 9 0.09697868447052116 1105 10-11 1.003257698634652 1105 12-13 -0.16981089704867358 1105 14-15 0.7824657511289459 1105 16-17 -0.27031488100458745 1105 18-19 -0.06799796515050716 1105 20-21 -0.14299191301273595 1105 22-23 0.25099299893377136 1105 24-25 -0.04066842559640094 1105 26-27 -0.03418223896992245 1105 28-29 0.3465493848536525 1105 30-31 0.13587931284558508 1105 32-33 0.6227518025663024 1105 34-35 0.6910529876892646 1105 36-37 0.03770580734884632 1105 38-39 0.5881736240737716 1105 40-41 0.22901188006636275 1105 42-43 1.0304703687431385 1105 44-45 0.779059429008587 1105 46-47 0.2860969688741335 1105 48-49 0.28701146110780584 1105 50-51 0.4105416128228079 1105 52-53 0.2438621557607199 1105 54-55 0.444699998064376 1105 56-57 0.3711294622923944 1105 58-59 0.7443783358141509 1105 60-61 0.4693312023024738 1105 62-63 0.8450197456507134 1105 64-65 0.5292881270245537 1105 66-67 0.8950935362270158 1105 68-69 0.48086802665460127 1105 70-71 0.34995358116838204 1105 72-73 0.6149493778496904 1105 74-75 0.9300163951616511 1105 76 0.372171747235452 1106 1 -0.182668835537104 1106 2 0.29396829679228276 1106 3 1.2170285408359156 1106 4 0.4099882285206675 1106 5 0.6144262948689239 1106 6 1.4433664127349566 1106 7 0.13279257819843693 1106 8 0.6688540699765753 1106 9 0.8415911861436562 1106 10-11 0.21742748848875237 1106 12-13 0.5052242388099835 1106 14-15 0.749521700801008 1106 16-17 0.7254353532344702 1106 18-19 0.7803489763566525 1106 20-21 0.8469274418259758 1106 22-23 0.6196685443761503 1106 24-25 0.7504656260700457 1106 26-27 0.6153375709618132 1106 28-29 0.72413438016887 1106 30-31 0.7773429723289205 1106 32-33 0.565898636992749 1106 34-35 1.1967918446026644 1106 36-37 0.965300841480822 1106 38-39 1.211751458366372 1106 40-41 1.1090731168485881 1106 42-43 0.852984088459376 1106 44-45 1.3398695615209668 1106 46-47 0.9386786179171018 1106 48-49 1.352305598438818 1106 50-51 1.1312700962922229 1106 52-53 0.8566733590802222 1106 54-55 1.1648177868476708 1106 56-57 0.8289831632695055 1106 58-59 1.0083155597466913 1106 60-61 1.3014821861065151 1106 62-63 1.3181491794614466 1106 64-65 0.9790187521082103 1106 66-67 0.6177377605611092 1106 68-69 1.2705464498911176 1106 70-71 1.0279919963738458 1106 72-73 1.0197479322600778 1106 74-75 1.1724287228286308 1106 76 1.069902974648116 1107 1 -0.15627173127295535 1107 2 0.5831534296606478 1107 3 0.24326673276670263 1107 4 -0.36718136625288267 1107 5 0.19096024030902115 1107 6 -0.02224672401793626 1107 7 -0.037190647721931924 1107 8 0.6109216960028832 1107 9 1.0324625554382614 1107 10-11 0.5886643561500939 1107 12-13 0.44866027659580254 1107 14-15 -0.2735912152403088 1107 16-17 0.2878525863941803 1107 18-19 0.5249327143278748 1107 20-21 0.30011893118767574 1107 22-23 0.6692668493112599 1107 24-25 0.17733946733427786 1107 26-27 0.5089457637754435 1107 28-29 0.3748009291226957 1107 30-31 0.049074233916275745 1107 32-33 0.3755658039389864 1107 34-35 0.22538723614499645 1107 36-37 0.09099166870780451 1107 38-39 0.12444678810946286 1107 40-41 0.077785044102054 1107 42-43 -0.18902173832618274 1107 44-45 0.5526266905048125 1107 46-47 -0.2895636351066493 1107 48-49 0.09221907950176345 1107 50-51 0.8701418187253473 1107 52-53 0.5015749217181664 1107 54-55 -0.08227323460549485 1107 56-57 0.14921800038436572 1107 58-59 -0.07694973556539253 1107 60-61 -0.46402500909080047 1107 62-63 0.49110759740088383 1107 64-65 0.5311155120623035 1107 66-67 0.2553028704754716 1107 68-69 0.1593254734631131 1107 70-71 -0.29090091437039334 1107 72-73 0.10262954257172296 1107 74-75 0.07650747271827285 1107 76 -0.10686051082906545 1108 1 0.1348502032737393 1108 2 0.34506063294185907 1108 3 -0.30862594432464263 1108 4 0.598126674420449 1108 5 0.6319070060955703 1108 6 0.7561861564400658 1108 7 0.6318343885935427 1108 8 0.4258238578417277 1108 9 0.3898922743137625 1108 10-11 0.5367545894236123 1108 12-13 0.354180228744827 1108 14-15 1.3716450489654513 1108 16-17 0.8430093837965522 1108 18-19 0.4638714479859125 1108 20-21 0.2927105743560965 1108 22-23 1.0224497938553938 1108 24-25 0.07541688338144681 1108 26-27 0.7030327500182878 1108 28-29 0.8892929397182705 1108 30-31 0.7259006886714694 1108 32-33 0.5858623091085988 1108 34-35 1.003512503170544 1108 36-37 0.31249042970925345 1108 38-39 0.9153209229466839 1108 40-41 0.49717323516417267 1108 42-43 0.8576500552310264 1108 44-45 0.54001453977423 1108 46-47 0.7727451746002636 1108 48-49 0.7739673491762069 1108 50-51 0.7724489194687472 1108 52-53 1.3450167742346153 1108 54-55 1.109617733522338 1108 56-57 0.9300263401263109 1108 58-59 0.5185556903937645 1108 60-61 0.7045300622584243 1108 62-63 0.737719577235115 1108 64-65 0.7287229631432197 1108 66-67 0.4988472608027408 1108 68-69 0.6267013599879334 1108 70-71 1.0350326069489242 1108 72-73 1.1851981147327066 1108 74-75 0.76970579793403 1108 76 0.1557900915805739 1109 1 0.6140456736799571 1109 2 0.1096270955134564 1109 3 0.7494753131643286 1109 4 -0.2178092002256733 1109 5 0.84498884163445 1109 6 -0.17667644149264916 1109 7 0.2904689267461542 1109 8 -0.15775636468773513 1109 9 0.47098714560219435 1109 10-11 0.354229406725608 1109 12-13 -0.01569270561556735 1109 14-15 0.137701076353693 1109 16-17 0.30603361185633204 1109 18-19 0.17691213532543415 1109 20-21 -0.00553157456746689 1109 22-23 0.6142267376963311 1109 24-25 0.2989997393956685 1109 26-27 0.231120510897874 1109 28-29 0.18026831663577525 1109 30-31 0.26021828693336957 1109 32-33 0.0602187512706891 1109 34-35 0.16560349006895692 1109 36-37 0.19268333246783342 1109 38-39 -0.21649321886647144 1109 40-41 0.16307454532283927 1109 42-43 0.012556566522093249 1109 44-45 -0.005413072313466216 1109 46-47 -0.09997521515547803 1109 48-49 0.2623882458777693 1109 50-51 0.0439035906123415 1109 52-53 -0.15880177866550937 1109 54-55 -0.052259282739431256 1109 56-57 -0.5065807439476906 1109 58-59 -0.47931283287966053 1109 60-61 -0.35549693096035284 1109 62-63 -0.2374762881938146 1109 64-65 -0.3278311220500072 1109 66-67 0.08018079164742886 1109 68-69 0.11508933813000866 1109 70-71 0.024794934949451175 1109 72-73 -0.2865577612301635 1109 74-75 -0.07338788828625908 1109 76 -0.14210641246840794 1110 1 -0.14453895831086783 1110 2 0.11569874092936416 1110 3 -0.004049614313238692 1110 4 -0.39984050293093176 1110 5 -0.10228903733742101 1110 6 -0.10612729489614381 1110 7 -0.2198191390974671 1110 8 0.09219303826713343 1110 9 0.032462555438261376 1110 10-11 0.5929546985019023 1110 12-13 0.45418518684884646 1110 14-15 0.22062120048510891 1110 16-17 0.15318837135171037 1110 18-19 0.03621018565262801 1110 20-21 -0.3073935458283472 1110 22-23 0.10093658050817922 1110 24-25 0.28638420947594767 1110 26-27 0.11465454584356394 1110 28-29 0.1604764391480913 1110 30-31 0.33515251077469443 1110 32-33 -0.16767034000637793 1110 34-35 -0.5661984393650314 1110 36-37 0.44673109607843386 1110 38-39 -0.15379504710636738 1110 40-41 0.4003662541537132 1110 42-43 0.3685826745973806 1110 44-45 0.001737976731934765 1110 46-47 0.12816686134724975 1110 48-49 0.2941716230618745 1110 50-51 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0.07866137422150743 2116 34-35 -0.10078995255399548 2116 36-37 -0.12698879867970803 2116 38-39 0.00686743687123581 2116 40-41 -0.06233529074273392 2116 42-43 0.1908352550466823 2116 44-45 0.09499676375211052 2116 46-47 0.20535265369698052 2116 48-49 0.4447652950580938 2116 50-51 0.29185441028446846 2116 52-53 -0.3616706311245288 2116 54-55 0.31864235660482976 2116 56-57 0.6553045019539496 2116 58-59 0.47560519990722483 2116 60-61 -0.3206608653865821 2116 62-63 -0.06124677999709505 2116 64-65 -0.15569997450902662 2116 66-67 0.3260824309916899 2116 68-69 0.1027942561627988 2116 70-71 -0.31741817980464404 2116 72-73 -0.49967251532851975 2116 74-75 -0.17379772435183227 2116 76 -0.39210641246840794 2117 1 0.02370432104352105 2117 2 0.1320527572833825 2117 3 -0.5292486950678743 2117 4 0.1023774437314593 2117 5 -0.5638891656042162 2117 6 -0.3460930904174937 2117 7 -0.33723670095947256 2117 8 0.013629626370388337 2117 9 -0.673165150189444 2117 10-11 -0.021817360714599943 2117 12-13 -0.3670162454636987 2117 14-15 0.016249440557793093 2117 16-17 -0.3058196987674684 2117 18-19 0.5298235681548995 2117 20-21 -1.256968662070129 2117 22-23 -1.0978235071407987 2117 24-25 -0.6981970528830921 2117 26-27 -0.4698144830699107 2117 28-29 -0.858790302340875 2117 30-31 -0.09381975153089783 2117 32-33 -1.0930350987044726 2117 34-35 -0.7251423760867439 2117 36-37 -0.5972002811862716 2117 38-39 -0.26600177042420015 2117 40-41 -0.8754340772021756 2117 42-43 -0.09269678668208314 2117 44-45 -0.22796647519473723 2117 46-47 -0.37616214298175166 2117 48-49 -0.22504167392920138 2117 50-51 -0.422590980390428 2117 52-53 -0.22231749791893307 2117 54-55 -0.27216915428865107 2117 56-57 -0.7016431951576863 2117 58-59 -0.5655469539498448 2117 60-61 -1.0007847032973025 2117 62-63 -0.9030252201361897 2117 64-65 -0.8027796707690484 2117 66-67 -0.9433892175783178 2117 68-69 -0.5681038348172613 2117 70-71 -0.035065969173746936 2117 72-73 -1.395279640443846 2117 74-75 -0.7643785915720471 2117 76 -0.5544795584481115 2118 1 -0.9135251161859159 2118 2 -0.6385100132793902 2118 3 -0.06958714449723402 2118 4 -1.50073156846846 2118 5 -0.09871797316029429 2118 6 -0.5102017090715663 2118 7 0.18716314162218595 2118 8 -0.4491802909849838 2118 9 -0.240264717289012 2118 10-11 -1.1002313709467941 2118 12-13 -1.3261858637242234 2118 14-15 -0.6606494181592453 2118 16-17 -0.9327382347769131 2118 18-19 -0.7936711189761532 2118 20-21 -0.9795188391657597 2118 22-23 -0.9269262226071504 2118 24-25 -0.5693107876331922 2118 26-27 -0.13293923710375566 2118 28-29 -0.9917104125337133 2118 30-31 -0.9529303379142142 2118 32-33 -1.0090130601369793 2118 34-35 -0.9073541037530184 2118 36-37 -0.7103447125125228 2118 38-39 -0.8261158987201505 2118 40-41 -1.0174057419011113 2118 42-43 -0.5596389354424112 2118 44-45 -1.1893989820817907 2118 46-47 -1.447590714410321 2118 48-49 -1.3411376991161355 2118 50-51 -0.8257787017599689 2118 52-53 -0.8351667698591143 2118 54-55 -1.0837590795149374 2118 56-57 -1.1866884529302126 2118 58-59 -1.0784355804748351 2118 60-61 -1.5611939909793193 2118 62-63 -0.7033007022298499 2118 64-65 -1.1316856133113546 2118 66-67 -0.8611381353272343 2118 68-69 -1.070268336981762 2118 70-71 -1.4042119746833883 2118 72-73 -0.9757597663785162 2118 74-75 -0.7145950417884954 2118 76 -0.6142985273579882 2119 1 0.7128231118572259 2119 2 0.024047767922461105 2119 3 -0.1100690047017423 2119 4 0.09630583172764418 2119 5 0.664869364001774 2119 6 0.08666059657348057 2119 7 0.3625028250512372 2119 8 0.2316573613472741 2119 9 0.7273778096755485 2119 10-11 0.49016994576422945 2119 12-13 0.6429624763794308 2119 14-15 0.7487041327582489 2119 16-17 -0.07164074213088512 2119 18-19 0.28697743124096675 2119 20-21 0.3744698147073322 2119 22-23 0.1509383242481519 2119 24-25 0.04889554378577543 2119 26-27 0.5111635784166282 2119 28-29 -0.056915623347549626 2119 30-31 -0.3386286149838291 2119 32-33 0.34616623668330604 2119 34-35 0.4934723425279728 2119 36-37 0.30653440220942585 2119 38-39 0.08810528071674639 2119 40-41 0.38042658755679426 2119 42-43 0.2185512872778581 2119 44-45 0.05984811134866064 2119 46-47 0.5038730760365162 2119 48-49 0.42462081047904476 2119 50-51 0.20554571342423245 2119 52-53 0.5711926642352942 2119 54-55 0.4054025677745976 2119 56-57 0.21865543276805965 2119 58-59 0.3853023380011429 2119 60-61 0.3156269923516781 2119 62-63 0.24804052203124982 2119 64-65 0.15254537142039837 2119 66-67 0.31663536236151657 2119 68-69 -0.7490048547013295 2119 70-71 -0.17130120286660855 2119 72-73 0.4013763871443885 2119 74-75 0.052931922772366136 2119 76 0.38296999764551387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 33.0 13 428.0 14 1510.0 15 2607.0 16 3524.0 17 4115.0 18 4211.0 19 3590.0 20 2861.0 21 2068.0 22 1429.0 23 1108.0 24 991.0 25 905.0 26 896.0 27 926.0 28 1122.0 29 1178.0 30 1522.0 31 1748.0 32 2188.0 33 2815.0 34 3690.0 35 5470.0 36 9957.0 37 30380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.089622228065565 18.032912612849504 15.543649750197213 31.333815408887723 2 18.95104741870453 17.34376369532825 34.32925760364625 29.375931282320977 3 18.578534490314663 18.49964939959681 25.780085897098783 37.14173021298974 4 15.518450346217898 20.81361206065387 24.1322640021036 39.535673591024626 5 17.038083968796563 31.87286352879306 27.309580156017176 23.7794723463932 6 32.88741344552546 27.0477254798843 15.304803225523708 24.760057849066527 7 28.447935840126213 22.75506179332106 19.60842317468665 29.188579191866072 8 24.22210535542116 31.194670873871505 16.9559119992988 27.627311771408536 9 22.877771934437725 17.24077482689105 16.67324042422649 43.208212814444735 10-11 26.71027259181348 20.943443772460338 22.350227890262072 29.996055745464105 12-13 24.761701288456482 17.99785257253046 22.583596283635725 34.65684985537734 14-15 25.092032605837495 18.94666491366465 20.33756245069682 35.623740029801034 16-17 26.789157682531332 20.115698133052852 20.063108072574284 33.03203611184153 18-19 26.075904987290738 18.61249890437374 20.743491980015776 34.568104128319746 20-21 25.94771671487422 19.146616706109214 21.46989219037602 33.435774388640546 22-23 27.92422648786046 18.989942150933473 19.53830309404856 33.5475282671575 24-25 26.554693662897712 20.736918222455955 18.689192742571652 34.01919537207468 26-27 25.477693049347007 21.067249539836972 20.89304496450171 32.56201244631431 28-29 26.370628451222718 20.14199316329214 22.348036637742133 31.13934174774301 30-31 26.086861249890436 19.19975457971777 22.458694889999123 32.254689280392675 32-33 25.007121570689804 20.418090980804628 22.697541414672624 31.87724603383294 34-35 24.784709439915854 20.414256288894734 22.425826102200016 32.375208168989396 36-37 25.470023665527215 18.37310456657025 23.56801647821895 32.58885528968358 38-39 23.60800683670786 18.183561223595408 26.768888596721887 31.439543342974847 40-41 23.466671049171705 18.42021649574897 26.3711762643527 31.741936190726623 42-43 21.60191515470243 18.334757647471296 26.536615829608206 33.52671136821807 44-45 20.367144359716015 18.11618020860724 29.060390919449556 32.45628451222719 46-47 20.824020510123585 19.88561661845911 28.43862301691647 30.85173985450083 48-49 20.642694364098517 20.632833727758786 27.138662459461827 31.58580944868087 50-51 20.414804102024718 21.943750547813128 25.501249013936366 32.140196336225785 52-53 19.169077044438602 22.002366552721536 26.061114032781134 32.767442370058724 54-55 19.54487685160838 20.93193969673065 25.891839775615743 33.631343676045226 56-57 20.114602506792885 21.43044964501709 25.063546323078274 33.39140152511175 58-59 20.54956613200105 21.558637917433604 24.318520466298533 33.57327548426681 60-61 20.099811552283285 20.05817775440442 25.30787097905163 34.53413971426067 62-63 20.452055394863706 20.48711543518275 24.69925059163818 34.36157857831537 64-65 20.917148742221052 21.037119817687792 23.72249978087475 34.32323165921641 66-67 20.49599272500178 22.96938255643876 21.767473965038377 34.767150753521086 68-69 20.505193268472258 24.51135068805329 21.528508195284424 33.454947848190024 70-71 20.038456481724953 25.14517047944605 22.85585940923832 31.960513629590675 72-73 20.236217021649576 24.149794022263126 24.60064422824086 31.013344727846437 74-75 19.733324568323255 26.101652204400033 23.707708826365153 30.45731440091156 76 18.701244631431326 25.718730826540448 22.046191603120345 33.53383293890788 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 1.5 14 2.0 15 4.0 16 8.5 17 12.0 18 12.0 19 19.0 20 28.5 21 31.0 22 39.0 23 66.0 24 104.5 25 124.0 26 155.5 27 233.5 28 317.0 29 354.0 30 405.0 31 557.5 32 762.0 33 865.0 34 984.5 35 1256.0 36 1520.0 37 1632.0 38 1762.0 39 2025.5 40 2379.0 41 2740.0 42 2881.0 43 2928.5 44 2984.5 45 3046.5 46 3100.0 47 3097.5 48 2899.5 49 2612.0 50 2520.0 51 2454.5 52 2314.0 53 2208.0 54 2177.0 55 2245.5 56 2392.0 57 2651.5 58 2833.0 59 2906.0 60 3145.5 61 3416.0 62 3520.0 63 3625.0 64 3613.0 65 3570.5 66 3709.0 67 3773.0 68 3683.0 69 3460.0 70 3241.0 71 3155.0 72 2934.5 73 2380.5 74 1748.5 75 1450.0 76 1205.5 77 728.5 78 351.0 79 206.0 80 137.0 81 56.5 82 31.5 83 18.0 84 11.0 85 5.5 86 9.5 87 12.0 88 9.5 89 9.0 90 7.0 91 2.5 92 2.0 93 5.5 94 7.5 95 7.0 96 8.0 97 6.5 98 6.0 99 14.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.478131299850995E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 91272.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.16307301253397 #Duplication Level Percentage of deduplicated Percentage of total 1 81.91362610560547 64.02620738013849 2 12.035155100152787 18.814094136208258 3 3.9514444709213494 9.265711280567974 4 1.283974152310733 4.014374616530809 5 0.4527550777252912 1.7694364098518716 6 0.15839419127850746 0.742834604259795 7 0.08830826593403512 0.48317118064685777 8 0.060273895796246195 0.37689543342974846 9 0.016820622082673355 0.1183276360767815 >10 0.039248118192904496 0.38894732228942064 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006573757559821194 0.0 2 0.0 0.0 0.0 0.00986063633973179 0.0 3 0.0 0.0 0.0 0.013147515119642388 0.0 4 0.0 0.0 0.0 0.08326759575773512 0.0 5 0.0 0.0 0.0 0.09531948461740732 0.0 6 0.0 0.0 0.0 0.09531948461740732 0.0 7 0.0 0.0 0.0 0.1007976159172583 0.0 8 0.0 0.0 0.0 0.1062757472171093 0.0 9 0.0 0.0 0.0 0.11723200981681128 0.0 10 0.0 0.0 0.0 0.11942326233675168 0.0 11 0.0 0.0 0.0 0.1270926461565431 0.0 12 0.0 0.0 0.0 0.1270926461565431 0.0 13 0.0 0.0 0.0 0.1292838986764835 0.0 14 0.0 0.0 0.0 0.13695328249627486 0.0 15 0.0 0.0 0.0 0.13804890875624506 0.0 16 0.0 0.0 0.0 0.13914453501621526 0.0 17 0.0 0.0 0.0 0.13914453501621526 0.0 18 0.0 0.0 0.0 0.14024016127618547 0.0 19 0.0 0.0 0.0 0.14571829257603647 0.0 20 0.0 0.0 0.0 0.14900517135594707 0.0 21 0.0 0.0 0.0 0.15777018143570865 0.0 22 0.0 0.0 0.0 0.16215268647558945 0.0 23 0.0 0.0 0.0 0.16982207029538085 0.0 24 0.0 0.0 0.0 0.18187395915505303 0.0 25 0.0 0.0 0.0 0.19173459549478483 0.0 26 0.0 0.0 0.0 0.2169339994740994 0.0 27 0.0 0.0 0.0 0.3418353931107021 0.0 28 0.0 0.0 0.0 0.5083705846261724 0.0 29 0.0 0.0 0.0 0.7231133315803313 0.0 30 0.0 0.0 0.0 0.9060829169953546 0.0 31 0.0 0.0 0.0 1.124112542729424 0.0 32 0.0 0.0 0.0 1.332281532123762 0.0 33 0.0 0.0 0.0 1.6346743798755368 0.0 34 0.0 0.0 0.0 2.113463055482514 0.0 35 0.0 0.0 0.0 2.759882548864931 0.0 36 0.0 0.0 0.0 3.5936541327022526 0.0 37 0.0 0.0 0.0 4.7714523621702165 0.0 38 0.0 0.0 0.0 5.897756157419581 0.0 39 0.0 0.0 0.0 6.965991760890525 0.0 40 0.0 0.0 0.0 7.948768516083794 0.0 41 0.0 0.0 0.0 8.934832150056973 0.0 42 0.0 0.0 0.0 9.85734946095188 0.0 43 0.0 0.0 0.0 10.757954246647383 0.0 44 0.0 0.0 0.0 11.77688666841967 0.0 45 0.0 0.0 0.0 12.77281093873258 0.0 46 0.0 0.0 0.0 13.793934613024804 0.0 47 0.0 0.0 0.0 14.687965641160487 0.0 48 0.0 0.0 0.0 15.465860285739328 0.0 49 0.0 0.0 0.0 16.19773862739942 0.0 50 0.0 0.0 0.0 16.967963888158472 0.0 51 0.0 0.0 0.0 17.61109650276098 0.0 52 0.0 0.0 0.0 18.247655359803662 0.0 53 0.0 0.0 0.0 18.853536681567185 0.0 54 0.0 0.0 0.0 19.48461740731002 0.0 55 0.0 0.0 0.0 20.112411254272942 0.0 56 0.0 0.0 0.0 20.81689893943378 0.0 57 0.0 0.0 0.0 21.42168463493733 0.0 58 0.0 0.0 0.0 22.067008502059778 0.0 59 0.0 0.0 0.0 22.664124813743534 0.0 60 0.0 0.0 0.0 23.308353054606012 0.0 61 0.0 0.0 0.0 23.827679901831885 0.0 62 0.0 0.0 0.0 24.34262424401788 0.0 63 0.0 0.0 0.0 24.971513717240775 0.0 64 0.0 0.0 0.0 25.54233499868525 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACCGT 20 0.0069316486 52.499996 6 TACTCTG 20 0.0069316486 52.499996 15 GTCTTGG 20 0.0069316486 52.499996 26 AAGGATT 20 0.0069316486 52.499996 20 ATGTACC 30 5.850838E-4 46.666664 11 CACATTT 40 0.00240782 34.999996 7 TACCCGA 45 0.0042879065 31.111109 14 TGTATAT 45 0.0042879065 31.111109 37 ACCCGAT 45 0.0042879065 31.111109 15 TGTACCC 45 0.0042879065 31.111109 12 GTACCCG 50 0.0071761785 27.999998 13 TACCCGT 50 0.0071761785 27.999998 1 CGAAGAA 65 7.9030154E-4 26.923077 15 AGCCGCC 90 1.9100086E-4 23.333332 12 TGTAGAT 85 0.003764477 20.588236 37 CCCGGAG 90 0.005240459 19.444443 2 CCAGGCC 115 9.983697E-4 18.26087 3 ATTGAAC 390 0.0 17.051283 70 AATTGAA 550 0.0 15.909091 70 TGAACCG 265 3.3744072E-8 15.849057 70 >>END_MODULE