FastQCFastQC Report
Wed 25 May 2016
SRR1780262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91272
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT1319314.454597247786834No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC36403.988079586291524No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG32173.52462967832413No Hit
CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG31023.398632658427557TruSeq Adapter, Index 12 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC8520.9334735734946096No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT4220.46235428170742393TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT2580.28267157507231133No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1970.2158383732141292No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1860.203786484354457No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1770.1939258480147252No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1250.13695328249627486RNA PCR Primer, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT1160.1270926461565431No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1020.1117538785169603RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT970.10627574721710929No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC950.10408449469716889TruSeq Adapter, Index 12 (95% over 21bp)
CTTATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC940.1029888684371987No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTT150.002218328470.036
TCTTAAT252.8200739E-670.01
CTAAGTC150.002218328470.03
TAAGTCT150.002218328470.04
CCTAAGT150.002218328470.02
AGTCTGG150.002218328470.06
ATCCAGG150.002218328470.023
TCTTTGC150.002218328470.018
AGGATTA150.002218328470.032
AAGGATT150.002218328470.031
TGACTAC150.002218328470.021
TCTTTAT750.060.6666681
CTATGAG200.006931648652.49999614
GAGTGAC200.006931648652.49999618
GGCAGAC200.006931648652.4999962
GGTTTCT200.006931648652.49999669
TGAGTGA200.006931648652.49999617
CTTGGTG200.006931648652.49999630
GGAAGAA200.006931648652.49999641
ACGATCG200.006931648652.49999641
Produced by FastQC (version 0.11.5)