##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780262_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 91272 Sequences flagged as poor quality 0 Sequence length 76 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.7390875624507 34.0 34.0 34.0 34.0 34.0 2 33.71204750635463 34.0 34.0 34.0 34.0 34.0 3 33.70664606889298 34.0 34.0 34.0 34.0 34.0 4 33.74442326233675 34.0 34.0 34.0 34.0 34.0 5 33.72506354632308 34.0 34.0 34.0 34.0 34.0 6 37.47235734946095 38.0 38.0 38.0 37.0 38.0 7 37.46358138311859 38.0 38.0 38.0 37.0 38.0 8 37.386569813305286 38.0 38.0 38.0 37.0 38.0 9 37.42407310018407 38.0 38.0 38.0 37.0 38.0 10-11 37.34077811376983 38.0 38.0 38.0 36.0 38.0 12-13 37.31700302392848 38.0 38.0 38.0 36.0 38.0 14-15 37.227161670610926 38.0 38.0 38.0 36.0 38.0 16-17 37.23939433780349 38.0 38.0 38.0 36.0 38.0 18-19 37.15879459198878 38.0 38.0 38.0 36.0 38.0 20-21 37.216999737049704 38.0 38.0 38.0 36.0 38.0 22-23 37.22486633359628 38.0 38.0 38.0 36.0 38.0 24-25 37.21827066351126 38.0 38.0 38.0 36.0 38.0 26-27 37.10475282671575 38.0 38.0 38.0 36.0 38.0 28-29 37.05383359628364 38.0 38.0 38.0 36.0 38.0 30-31 37.04607108423175 38.0 38.0 38.0 36.0 38.0 32-33 36.9155655622754 38.0 38.0 38.0 35.0 38.0 34-35 36.92792422648786 38.0 38.0 38.0 35.5 38.0 36-37 36.92003571741607 38.0 38.0 38.0 35.0 38.0 38-39 35.90712376194233 38.0 37.0 38.0 32.0 38.0 40-41 36.432690200718724 38.0 37.0 38.0 34.0 38.0 42-43 36.884203260583746 38.0 37.0 38.0 35.0 38.0 44-45 36.98101827504601 38.0 37.5 38.0 35.5 38.0 46-47 36.92147646594793 38.0 37.5 38.0 35.5 38.0 48-49 36.95176505390481 38.0 38.0 38.0 35.5 38.0 50-51 36.731829038478395 38.0 37.0 38.0 34.0 38.0 52-53 36.70712266631607 38.0 37.0 38.0 34.0 38.0 54-55 36.6837529581909 38.0 37.0 38.0 34.5 38.0 56-57 36.74915088964852 38.0 37.0 38.0 34.0 38.0 58-59 36.54679967569463 38.0 37.0 38.0 34.0 38.0 60-61 36.503380007012005 38.0 37.0 38.0 34.0 38.0 62-63 36.50506727145236 38.0 37.0 38.0 34.0 38.0 64-65 36.29675585064423 38.0 37.0 38.0 33.5 38.0 66-67 35.746822683846084 38.0 37.0 38.0 31.0 38.0 68-69 35.50853492856517 38.0 37.0 38.0 31.0 38.0 70-71 34.09507844684022 38.0 34.5 38.0 24.0 38.0 72-73 31.667795161714437 38.0 26.0 38.0 11.0 38.0 74-75 30.975578490665264 38.0 24.0 38.0 11.0 38.0 76 30.388651503199227 37.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13342347809810917 1101 2 -0.08043092209865677 1101 3 -0.06356308899908925 1101 4 0.11182732297967846 1101 5 -0.07292421036085983 1101 6 -0.12004694454167719 1101 7 0.05292803916342592 1101 8 0.004030478108823843 1101 9 0.21806124376455216 1101 10-11 0.16608798923959256 1101 12-13 0.014719777786126542 1101 14-15 0.30945759079654067 1101 16-17 0.2288462978294632 1101 18-19 -0.005698953735858936 1101 20-21 0.1844426943876485 1101 22-23 0.16887614718717714 1101 24-25 0.15834697294170041 1101 26-27 0.125506947904924 1101 28-29 -0.5261933442723787 1101 30-31 -0.3859684666555623 1101 32-33 0.04499481587095033 1101 34-35 0.16357026405015773 1101 36-37 0.28672058692489344 1101 38-39 0.46364662138078927 1101 40-41 0.2612007515278094 1101 42-43 0.05645489327149278 1101 44-45 -0.14683182610387746 1101 46-47 -0.21893375766241974 1101 48-49 -0.055385080699217326 1101 50-51 -0.019840823560222987 1101 52-53 0.2576877703725273 1101 54-55 0.05467771533504617 1101 56-57 -0.18408353865661553 1101 58-59 -0.27354721097327683 1101 60-61 0.1198858942189105 1101 62-63 -0.1716933526276918 1101 64-65 -0.3527839166629789 1101 66-67 -0.3283367361235747 1101 68-69 -0.6849526206010097 1101 70-71 -0.7480864995482079 1101 72-73 -0.2988456233526051 1101 74-75 -0.10082682292322076 1101 76 -0.5516594744116823 1102 1 -0.10156133637136833 1102 2 -0.14823468506683213 1102 3 -0.11933942098431771 1102 4 0.1575807958124571 1102 5 -0.19882538848640507 1102 6 -0.060138381151489284 1102 7 -0.2435727521149289 1102 8 -0.2713871410225295 1102 9 -0.015893389609807684 1102 10-11 -0.0360836302434393 1102 12-13 0.05501342997533243 1102 14-15 0.0049385121962188805 1102 16-17 -0.02630580345417144 1102 18-19 -0.09234393351430015 1102 20-21 -0.0664277118019001 1102 22-23 -0.19933204693978013 1102 24-25 -0.05228253294892937 1102 26-27 0.04515702703276503 1102 28-29 -0.016170485119928912 1102 30-31 0.02306969054507846 1102 32-33 0.32456225037068975 1102 34-35 0.3258087025942089 1102 36-37 0.18706347421168346 1102 38-39 -0.024150108002018555 1102 40-41 0.24779297941597633 1102 42-43 0.17316845050729057 1102 44-45 0.2622766669504557 1102 46-47 -0.12024376293760497 1102 48-49 0.0017188405246244542 1102 50-51 -0.24901261888287962 1102 52-53 -0.03197285913336856 1102 54-55 0.04340639477938879 1102 56-57 -0.09101162024620635 1102 58-59 -0.04533374165729498 1102 60-61 0.14342202926375336 1102 62-63 0.1506998247944935 1102 64-65 0.33540845189688895 1102 66-67 0.20584648877532175 1102 68-69 -0.4533806142707846 1102 70-71 -0.254267262692224 1102 72-73 -0.5110840618966499 1102 74-75 -0.7646741941206869 1102 76 -0.3058356657252048 1103 1 -0.08635336997114962 1103 2 0.06847036844895626 1103 3 -0.01759169032451524 1103 4 0.06010077955449589 1103 5 0.09205660582330921 1103 6 0.12086690268539257 1103 7 0.02495454770894412 1103 8 0.1580939590994106 1103 9 0.07488021830239688 1103 10-11 0.16648038547258892 1103 12-13 0.2487710508939074 1103 14-15 0.14655211468910778 1103 16-17 0.12157388764460109 1103 18-19 0.1777244853991249 1103 20-21 0.08204471740823038 1103 22-23 0.07727342657329928 1103 24-25 -0.12927074592820276 1103 26-27 -0.13005635868733378 1103 28-29 -0.11060213394799234 1103 30-31 0.21935068228061283 1103 32-33 0.1754970830491942 1103 34-35 -0.07539032192821082 1103 36-37 0.21526087293813845 1103 38-39 -0.019035624869651713 1103 40-41 -0.07059206326116652 1103 42-43 0.14439524904407364 1103 44-45 0.1254388400977291 1103 46-47 -0.13260658640325573 1103 48-49 0.2604066876998061 1103 50-51 0.03599280043699338 1103 52-53 0.21267556248972141 1103 54-55 -0.10606522153276643 1103 56-57 -0.32593913684286946 1103 58-59 0.2396547422879678 1103 60-61 0.03572998211566869 1103 62-63 0.20516398954182336 1103 64-65 0.18549651584486782 1103 66-67 -0.8394895090569534 1103 68-69 -0.15985670152183218 1103 70-71 -0.9389305874449647 1103 72-73 -0.6242292996693131 1103 74-75 -1.1168453091457522 1103 76 -1.3716804022107745 1104 1 0.03606862897120777 1104 2 0.003491521278142784 1104 3 0.05598049370509983 1104 4 0.16421236072847734 1104 5 0.18393920762130023 1104 6 0.26847398888316576 1104 7 0.2143384715587544 1104 8 -0.20533808743681448 1104 9 0.23187387245364022 1104 10-11 0.25938757743450935 1104 12-13 0.14908834333176912 1104 14-15 0.31808833890909227 1104 16-17 0.11582296220831978 1104 18-19 0.13422898037532605 1104 20-21 0.19640220022155574 1104 22-23 0.06266475391332449 1104 24-25 -0.0012961293232365279 1104 26-27 0.14291429176216042 1104 28-29 -0.004573577628590897 1104 30-31 0.13870552099029254 1104 32-33 0.11845054682497391 1104 34-35 -0.053940031076813 1104 36-37 0.17351708658171816 1104 38-39 0.440542640598359 1104 40-41 0.49755970828827856 1104 42-43 0.05598303328521581 1104 44-45 0.02169082317546156 1104 46-47 0.06705629038150818 1104 48-49 0.3257490991223335 1104 50-51 0.18293886072255106 1104 52-53 0.14577974017348083 1104 54-55 -0.2606791824000325 1104 56-57 -0.038458967620236706 1104 58-59 0.293825815794051 1104 60-61 0.004992277197636952 1104 62-63 -0.0191882050642036 1104 64-65 -0.3348961335469838 1104 66-67 -0.9763549241812299 1104 68-69 -0.25636648470533174 1104 70-71 -0.5735154631720931 1104 72-73 0.40293886532275636 1104 74-75 0.338998159918237 1104 76 0.05626777335770683 1105 1 -0.024415241996535997 1105 2 0.08010442450395061 1105 3 0.1850127517696123 1105 4 0.18437365105106096 1105 5 0.08313275600839631 1105 6 -0.042009882084570904 1105 7 -0.15259701231221356 1105 8 0.028532880305121466 1105 9 0.058486775679448044 1105 10-11 0.15858112582161255 1105 12-13 -0.02833101150693551 1105 14-15 -0.0427987578650999 1105 16-17 0.16622618801476818 1105 18-19 0.34592252876242213 1105 20-21 0.11575703893123546 1105 22-23 0.17153572165526043 1105 24-25 -0.037586451903877105 1105 26-27 0.0723497756331355 1105 28-29 -5.413195640784352E-4 1105 30-31 0.26572165002254877 1105 32-33 0.2111924823088458 1105 34-35 0.13557609795545034 1105 36-37 0.15940418335591033 1105 38-39 0.04336522124351916 1105 40-41 -0.32300480784075347 1105 42-43 -0.05490406348897636 1105 44-45 0.09628759736901316 1105 46-47 -0.041814677360427766 1105 48-49 -0.012960578297025904 1105 50-51 -0.11544823605164112 1105 52-53 0.29698941759284025 1105 54-55 -0.07721144046454498 1105 56-57 0.34057329044427576 1105 58-59 0.16479355772953141 1105 60-61 0.48079872881054087 1105 62-63 0.23282792396805263 1105 64-65 -0.016347746450215084 1105 66-67 -0.7848226661167104 1105 68-69 -0.16765680728598653 1105 70-71 0.18051679489242645 1105 72-73 0.7093904782259806 1105 74-75 0.463998159918237 1105 76 0.4272355152931908 1106 1 0.05878981756710999 1106 2 0.1072092297509073 1106 3 0.17770114689744076 1106 4 -0.02543761919388743 1106 5 0.17674472227796656 1106 6 0.09097097723814329 1106 7 0.039579403160949767 1106 8 0.14812865061698943 1106 9 -0.11786177203839543 1106 10-11 0.1373071999750053 1106 12-13 0.02746807830700959 1106 14-15 0.07225444786372748 1106 16-17 0.09489202926761209 1106 18-19 0.10704051832205153 1106 20-21 0.01090159789254841 1106 22-23 -0.043713241000347125 1106 24-25 0.02922187362148776 1106 26-27 -0.07672665322511563 1106 28-29 0.22250445744034408 1106 30-31 -0.31488168286461615 1106 32-33 0.09089399482390093 1106 34-35 -0.17752623105003806 1106 36-37 0.13544487402650418 1106 38-39 -0.1491458214549155 1106 40-41 0.14444432882100244 1106 42-43 -0.009361298129590523 1106 44-45 0.14067589880122 1106 46-47 -0.17933810126619676 1106 48-49 -0.16657957173832472 1106 50-51 0.060030280880503994 1106 52-53 0.13463665357195964 1106 54-55 -0.013707558788738083 1106 56-57 -0.06959603093171296 1106 58-59 0.08529449468576189 1106 60-61 0.18756489130552012 1106 62-63 0.2756183803995853 1106 64-65 0.37706511662032227 1106 66-67 0.68834023041655 1106 68-69 0.7019724257484228 1106 70-71 0.3838195656058474 1106 72-73 0.9559438178056929 1106 74-75 0.9125659553940757 1106 76 0.4370400929936338 1107 1 0.007002053816783871 1107 2 -0.07617560294971781 1107 3 0.051330528022184296 1107 4 0.14586987617116876 1107 5 0.13133110878807486 1107 6 -0.026429923265077093 1107 7 0.07845995405703832 1107 8 0.20403047810882669 1107 9 0.1627420948283813 1107 10-11 0.012896499877889767 1107 12-13 0.11471977778612796 1107 14-15 0.14350014398802102 1107 16-17 0.17139948931881577 1107 18-19 -0.2120819324592702 1107 20-21 0.1589107794940361 1107 22-23 -0.11835789536601737 1107 24-25 0.03494271762254186 1107 26-27 0.20423035216024488 1107 28-29 0.03338112381273106 1107 30-31 -0.01788336027257742 1107 32-33 -0.006069013916281563 1107 34-35 -0.09387654446047833 1107 36-37 0.17395462947808227 1107 38-39 0.08917853627440309 1107 40-41 -0.002629035706235072 1107 42-43 0.10326340390979283 1107 44-45 0.11487030155569755 1107 46-47 -0.16574226830071837 1107 48-49 0.02972130227950487 1107 50-51 -0.1879259299431979 1107 52-53 0.16407074909592723 1107 54-55 0.10148622597333912 1107 56-57 -0.03514736844385169 1107 58-59 0.34560172519693566 1107 60-61 -0.23968857386619646 1107 62-63 -0.06318271432981959 1107 64-65 0.038705445039148856 1107 66-67 0.029110072387062758 1107 68-69 -0.18069730145207785 1107 70-71 0.14978584087732116 1107 72-73 0.3224309723920733 1107 74-75 0.18853487920443968 1107 76 -0.1686807510074253 1108 1 0.02087883754744979 1108 2 0.08094650249332602 1108 3 0.05693592834688843 1108 4 0.09232804928917204 1108 5 0.14018353978867992 1108 6 -0.1039997771487009 1108 7 0.1031032079235672 1108 8 0.11125084895390813 1108 9 0.03238235800876765 1108 10-11 0.01005962044526143 1108 12-13 -0.12784195852080416 1108 14-15 -0.10173612021773693 1108 16-17 -0.03418027655317957 1108 18-19 -0.21226991844577014 1108 20-21 -0.001988135314135775 1108 22-23 -0.20948837473028448 1108 24-25 -0.16271600236377992 1108 26-27 0.03558983263533122 1108 28-29 -0.10309670238568458 1108 30-31 -0.2334605626993067 1108 32-33 -0.49191025120586573 1108 34-35 -0.05521124496461738 1108 36-37 -0.001367937385118978 1108 38-39 -0.0949622823191234 1108 40-41 -0.054017568561050666 1108 42-43 -0.1243107223826172 1108 44-45 0.0359332768611722 1108 46-47 -0.01706244261499279 1108 48-49 0.10782093485234867 1108 50-51 0.05076500400536332 1108 52-53 0.1504759443450041 1108 54-55 -0.6981548916888016 1108 56-57 -0.19995980969823535 1108 58-59 -0.1713170135879949 1108 60-61 0.11342601213740267 1108 62-63 -0.5028648471162853 1108 64-65 -0.16971120026547482 1108 66-67 -0.16149062828797156 1108 68-69 -0.08623434323623513 1108 70-71 0.28809225803532 1108 72-73 0.864915805340889 1108 74-75 1.1248674435041899 1108 76 0.9301827882560119 1109 1 0.19233515461901618 1109 2 0.14633922090796858 1109 3 0.19962142442957997 1109 4 0.002723730374171396 1109 5 0.1494336550036337 1109 6 0.1126701813739146 1109 7 -0.07750446868450211 1109 8 -0.15747981139239897 1109 9 -0.002988634156615433 1109 10-11 -0.22491305715035992 1109 12-13 -0.10573714582740479 1109 14-15 -0.23049706563875816 1109 16-17 -0.13047529268327196 1109 18-19 0.15108514113682503 1109 20-21 0.05950373380166951 1109 22-23 -0.3346118193283445 1109 24-25 -0.13951664756754667 1109 26-27 -0.097269473441429 1109 28-29 0.07997163654380302 1109 30-31 0.022629370805198334 1109 32-33 -7.324251475324672E-4 1109 34-35 0.014773612498018451 1109 36-37 0.03903136474142599 1109 38-39 -0.39198644454177867 1109 40-41 -0.06239534194969565 1109 42-43 -0.24085065259527028 1109 44-45 -0.10816771727932206 1109 46-47 0.10509833162952731 1109 48-49 0.016554228683411054 1109 50-51 -0.1935153962843188 1109 52-53 0.13685589035857504 1109 54-55 -0.22286850022128846 1109 56-57 0.13512643692761372 1109 58-59 0.225310479992892 1109 60-61 0.1607299821156687 1109 62-63 0.10680333380411611 1109 64-65 -0.23458545136824682 1109 66-67 -0.39891573856514384 1109 68-69 -0.313545226111998 1109 70-71 0.10205301911241804 1109 72-73 -0.7143932340955423 1109 74-75 -0.2787305550473924 1109 76 0.12831959778922553 1110 1 -0.06717708986590765 1110 2 -0.09704943186137172 1110 3 -0.01475825579709067 1110 4 -0.023047031280015062 1110 5 0.11182318478842745 1110 6 -0.0700863456449099 1110 7 -0.08818044520747037 1110 8 -0.03724436647571139 1110 9 0.08112052053541419 1110 10-11 0.014174846782715633 1110 12-13 0.008257996856258387 1110 14-15 -0.06182338284850175 1110 16-17 0.10863790977236931 1110 18-19 0.14612663071383736 1110 20-21 0.21696173825421283 1110 22-23 -0.04297045118424592 1110 24-25 0.25371515436188474 1110 26-27 0.15163425724735902 1110 28-29 0.17716012740260112 1110 30-31 0.14219018845345488 1110 32-33 0.0789410552545462 1110 34-35 0.15421906888336423 1110 36-37 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64-65 0.4304497742109419 2115 66-67 0.3546068193538332 2115 68-69 0.3833962452333495 2115 70-71 -0.09724246930379365 2115 72-73 0.10255230173970986 2115 74-75 -0.2212857643686199 2115 76 0.061932929316117935 2116 1 0.1021712201927869 2116 2 0.23650315533419786 2116 3 0.015195194921382438 2116 4 0.170756517259413 2116 5 -0.05138601712751267 2116 6 0.05529313219359011 2116 7 0.0577414329548418 2116 8 0.05973330336170335 2116 9 -0.03577551940252022 2116 10-11 0.13779186088242312 2116 12-13 0.28565629679555116 2116 14-15 0.16499473763992967 2116 16-17 -0.38047529268327196 2116 18-19 -0.062029612961538305 2116 20-21 0.1496676682278988 2116 22-23 0.29858490198312637 2116 24-25 -0.12107402461673189 2116 26-27 0.05436987082086375 2116 28-29 0.16398802998642736 2116 30-31 0.09639986260848588 2116 32-33 0.021808558459028404 2116 34-35 0.12133098954719657 2116 36-37 0.02878546310207497 2116 38-39 -0.07026513306637128 2116 40-41 -0.031657637031656805 2116 42-43 0.22636246215883205 2116 44-45 -0.10202017629572424 2116 46-47 0.2485409545803492 2116 48-49 -0.0039375745952767716 2116 50-51 0.2675501774861786 2116 52-53 0.17579031658807764 2116 54-55 0.4472134669918262 2116 56-57 0.13922479758335982 2116 58-59 0.2499006439273117 2116 60-61 0.04187752309927362 2116 62-63 0.3281148092139503 2116 64-65 0.060496515844867815 2116 66-67 0.40846131061517355 2116 68-69 0.43235641323226304 2116 70-71 0.4094300682927354 2116 72-73 -0.09758995540701676 2116 74-75 0.14749895314932715 2116 76 0.0791392699203719 2117 1 -0.018916708272193716 2117 2 -0.18640863233168403 2117 3 0.04280146842929611 2117 4 -0.05532750800493602 2117 5 0.08098570950650696 2117 6 0.07661880106024199 2117 7 0.21805652769617012 2117 8 -0.41365396510753527 2117 9 -0.1298249126322375 2117 10-11 0.17239728278293143 2117 12-13 0.041928030526293014 2117 14-15 0.19954693407737523 2117 16-17 0.03990828548718639 2117 18-19 -0.061745743825206034 2117 20-21 0.09374544837125853 2117 22-23 0.1450205701401046 2117 24-25 0.023770202194711487 2117 26-27 0.016526566584118996 2117 28-29 -0.671030776343855 2117 30-31 0.0992208121507403 2117 32-33 -0.1406089420732286 2117 34-35 -0.374801293470334 2117 36-37 -0.143416651722994 2117 38-39 -0.6252913909614861 2117 40-41 -0.42093980295317124 2117 42-43 -0.23326602410899966 2117 44-45 -0.07263084977524414 2117 46-47 0.05872121148191667 2117 48-49 -0.02494389068355929 2117 50-51 -0.13094886445549747 2117 52-53 -0.16538490163632247 2117 54-55 0.0026743995019415934 2117 56-57 -0.6634240562950069 2117 58-59 0.020880347284069956 2117 60-61 -0.5491202769149126 2117 62-63 -0.24255086391073633 2117 64-65 -0.5848001274025947 2117 66-67 -0.04917122078784786 2117 68-69 -0.28523815067377 2117 70-71 -0.2712881206221738 2117 72-73 -0.675192680550726 2117 74-75 -0.7912385388668497 2117 76 -1.1440883221596785 2118 1 0.10918246528152764 2118 2 -0.34658179250484267 2118 3 -0.23504190032316075 2118 4 -0.3424192041875429 2118 5 -0.15750307443681777 2118 6 0.08035748661710329 2118 7 0.10766691730655964 2118 8 0.2451417845973083 2118 9 0.013032230224901298 2118 10-11 -0.08567040710293838 2118 12-13 -0.16812706589243476 2118 14-15 -0.2388631406963384 2118 16-17 0.06666940315739822 2118 18-19 -0.12746790045645184 2118 20-21 -0.14700622419859855 2118 22-23 0.0692629943825338 2118 24-25 -0.13699327675058726 2118 26-27 -0.05814875809121389 2118 28-29 0.012755134714787175 2118 30-31 -0.2165997309425265 2118 32-33 -0.8014708074805483 2118 34-35 -0.32295162798430255 2118 36-37 -0.5360770216560837 2118 38-39 -0.11299308320862878 2118 40-41 -0.1323723098402212 2118 42-43 0.07812712819324474 2118 44-45 -0.031111762801614873 2118 46-47 0.1524835097896613 2118 48-49 -0.25447950658281115 2118 50-51 -0.14983906516387435 2118 52-53 0.11265524004018346 2118 54-55 0.30993532039921945 2118 56-57 -0.008366992147038843 2118 58-59 0.15921171288815827 2118 60-61 0.03598401273482921 2118 62-63 -0.09723405950302322 2118 64-65 -0.1397981596733544 2118 66-67 -0.23010392444782468 2118 68-69 -0.06701697790715144 2118 70-71 -0.8431102378988342 2118 72-73 -0.8271997643759939 2118 74-75 -0.6221122106496146 2118 76 -1.2190588062210708 2119 1 -0.17839282537542545 2119 2 -0.011065613768330707 2119 3 0.21761253307087003 2119 4 -0.12057440799760144 2119 5 -0.029018692870778295 2119 6 -0.12579883779807943 2119 7 0.11421545351610973 2119 8 -0.03570987529903391 2119 9 0.03306304686589101 2119 10-11 -0.08810422136264151 2119 12-13 -0.2768958010094025 2119 14-15 -0.4109985938410432 2119 16-17 -0.2604072182181483 2119 18-19 -0.10374258878815112 2119 20-21 -0.012113382063844824 2119 22-23 0.19060351826543354 2119 24-25 0.02337582256304671 2119 26-27 0.24539515562219805 2119 28-29 0.1157107307366374 2119 30-31 0.026554072111117932 2119 32-33 -0.15164240014058805 2119 34-35 -0.02937059422607291 2119 36-37 -0.1313284574314011 2119 38-39 -0.19891197941257843 2119 40-41 0.006512688058649019 2119 42-43 -0.22275534834408717 2119 44-45 -0.15651448026903836 2119 46-47 0.043101943744005666 2119 48-49 -0.035821431222117894 2119 50-51 0.10215701271818034 2119 52-53 0.11598203651027461 2119 54-55 -0.014164693608336165 2119 56-57 0.13766186343497822 2119 58-59 -0.04580649694515415 2119 60-61 -0.09882128212439767 2119 62-63 -0.022468686201442267 2119 64-65 0.07369462643114133 2119 66-67 0.3951937362889808 2119 68-69 0.7929774190672205 2119 70-71 0.81638338865951 2119 72-73 1.0029935400083794 2119 74-75 0.641212484685866 2119 76 0.23737766947580496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 3.0 22 6.0 23 10.0 24 32.0 25 59.0 26 124.0 27 228.0 28 407.0 29 677.0 30 1018.0 31 1652.0 32 2584.0 33 4307.0 34 7198.0 35 12475.0 36 16318.0 37 44174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.564203698834255 10.833552458585327 12.05846261723201 44.54378122534841 2 17.84994302743448 15.338767639582784 40.05171355947059 26.75957577351214 3 18.139188360066612 11.853580506617583 36.72320098168113 33.284030151634674 4 14.932290297133841 28.13896923481462 26.733280743272854 30.195459724778683 5 16.00929091068455 24.357963011657464 43.26408975370322 16.368656323954774 6 28.68459111227978 38.52660180559207 13.431282320974669 19.357524761153474 7 26.55250241037777 19.687308265404504 19.564598124287844 34.19559119992988 8 22.24230870365501 39.742746954158996 11.249890437374004 26.765053904811992 9 19.461609255850647 13.70628451222719 11.039530195459726 55.792576036462435 10-11 26.572223683057235 27.198374090630207 20.56654833903059 25.662853887281972 12-13 24.594618283811027 13.710119204137083 25.8748575685862 35.82040494346568 14-15 21.433736523797002 11.762095713910071 16.616815671838022 50.18735209045491 16-17 32.61679375931282 22.49375493031817 12.261153475326497 32.62829783504251 18-19 37.33346480848453 19.26220527653607 12.25403190463669 31.150298010342713 20-21 26.799018318871067 15.11252081689894 12.432071171881848 45.65638969234815 22-23 23.482557629941276 22.686585152072926 13.903497239021826 39.92735997896398 24-25 32.933977561574196 25.191734595494786 11.968073450784468 29.906214392146552 26-27 34.57741695152949 25.80473748794811 16.752673328074327 22.865172232448067 28-29 27.04553422736436 19.898216320448768 20.881540888772022 32.17470856341485 30-31 22.455408011219212 25.80418967481813 18.254229117363487 33.486173196599175 32-33 21.644644578841266 16.17144359716014 33.165154702427905 29.018757121570687 34-35 31.693180822157945 20.942895959330354 23.679222543605924 23.684700674905777 36-37 25.98935051275309 34.89186168814094 16.03887281970374 23.079914979402226 38-39 20.760474187045315 22.594552546235427 26.68890787974406 29.956065386975194 40-41 22.03194846174073 12.532320974669119 28.68787799105969 36.74785257253046 42-43 31.548010342711898 12.011898501183277 27.035125777894642 29.404965378210186 44-45 21.299522306950653 22.62687352090455 31.999956174949602 24.073647997195195 46-47 28.3860329564379 15.932049259356647 26.286813042334998 29.395104741870455 48-49 28.55585502673328 13.364449119116486 19.054036287141727 39.0256595670085 50-51 23.5477473924095 14.196029450433867 27.25425102988868 35.00197212726795 52-53 16.923043211499696 14.273271101761766 41.734047681654836 27.069638005083707 54-55 18.32215794548164 15.78085283548076 32.781137698308356 33.11585152072925 56-57 26.340498729073538 17.996209133140503 22.00620124463143 33.65709089315453 58-59 20.296476465947936 18.6618020860724 33.303751424314136 27.737970023665525 60-61 26.927754404417563 26.457182925760364 22.928170742396354 23.686891927425716 62-63 19.23864931194671 41.71651766149531 15.325620124463143 23.71921290209484 64-65 16.582851257778948 45.06639495135419 14.472675081076344 23.878078709790515 66-67 17.139977210973793 44.39203698834254 14.580594267683407 23.88739153300026 68-69 16.798689630993074 45.08392497151372 14.771781050048208 23.345604347445 70-71 16.45411517223245 42.70696380050837 15.790165658690508 25.048755368568678 72-73 16.401525111753877 37.23431063195723 18.354478920150758 28.009685336138133 74-75 16.712682969585416 34.579060390919445 20.235121395389605 28.473135244105528 76 17.079717766675433 35.52896835831361 19.594180033307037 27.797133841703918 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 1.5 16 1.0 17 2.0 18 1.5 19 1.0 20 3.0 21 5.0 22 6.5 23 14.0 24 18.0 25 16.0 26 37.0 27 55.0 28 78.0 29 104.0 30 120.0 31 148.5 32 211.5 33 262.0 34 295.5 35 409.0 36 665.5 37 842.0 38 3684.0 39 8109.0 40 8701.5 41 6248.5 42 4786.0 43 3801.5 44 2437.0 45 1815.5 46 1574.0 47 1542.0 48 1439.0 49 1286.5 50 1205.0 51 1141.0 52 1050.5 53 1046.5 54 1069.0 55 1296.0 56 1596.0 57 1852.0 58 2035.0 59 2205.5 60 2588.0 61 2971.5 62 3143.0 63 3330.0 64 3507.5 65 3632.5 66 3767.0 67 3767.0 68 3692.0 69 3513.0 70 3271.0 71 3133.0 72 2936.0 73 2446.0 74 1849.0 75 1545.0 76 1242.5 77 702.0 78 320.0 79 176.0 80 118.5 81 42.5 82 14.0 83 4.0 84 3.0 85 1.5 86 0.5 87 0.0 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 91272.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.334516609694106 #Duplication Level Percentage of deduplicated Percentage of total 1 63.0643766217719 27.959286528179504 2 23.659953045842087 20.97905162590937 3 8.498702582478685 11.303576124112542 4 2.6665019152353886 4.728722938031379 5 1.0502903743976275 2.3282058024366727 6 0.4176448782898802 1.1109650276097818 7 0.19770171753367105 0.6135507055833114 8 0.13591993080439888 0.48207555438688754 9 0.07413814407512664 0.29581909019195374 >10 0.17793154578030396 1.3421421684634938 >50 0.02471271469170888 0.8228153212376195 >100 0.019770171753367108 1.7343763695328247 >500 0.0024712714691708885 0.9334735734946096 >1k 0.0074138144075126654 10.911341923043212 >5k 0.0 0.0 >10k+ 0.0024712714691708885 14.454597247786834 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 13193 14.454597247786834 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 3640 3.988079586291524 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 3217 3.52462967832413 No Hit CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG 3102 3.398632658427557 TruSeq Adapter, Index 12 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 852 0.9334735734946096 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 422 0.46235428170742393 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT 258 0.28267157507231133 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 197 0.2158383732141292 No Hit CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 186 0.203786484354457 No Hit CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 177 0.1939258480147252 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT 125 0.13695328249627486 RNA PCR Primer, Index 12 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT 116 0.1270926461565431 No Hit CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC 102 0.1117538785169603 RNA PCR Primer, Index 12 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT 97 0.10627574721710929 No Hit CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC 95 0.10408449469716889 TruSeq Adapter, Index 12 (95% over 21bp) CTTATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 94 0.1029888684371987 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00986063633973179 0.0 2 0.0 0.0 0.0 0.013147515119642388 0.0 3 0.0 0.0 0.0 0.016434393899552986 0.0 4 0.0 0.0 0.0 0.08874572705758611 0.0 5 0.0 0.0 0.0 0.1007976159172583 0.0 6 0.0 0.0 0.0 0.1018932421772285 0.0 7 0.0 0.0 0.0 0.1073713734770795 0.0 8 0.0 0.0 0.0 0.1128495047769305 0.0 9 0.0 0.0 0.0 0.12380576737663249 0.0 10 0.0 0.001095626259970199 0.0 0.1303795249364537 0.0 11 0.0 0.001095626259970199 0.0 0.13914453501621526 0.0 12 0.0 0.001095626259970199 0.0 0.13914453501621526 0.0 13 0.0 0.001095626259970199 0.0 0.14133578753615567 0.0 14 0.0 0.001095626259970199 0.0 0.14571829257603647 0.0 15 0.0 0.001095626259970199 0.0 0.14790954509597687 0.0 16 0.0 0.001095626259970199 0.0 0.14900517135594707 0.0 17 0.0 0.001095626259970199 0.0 0.14900517135594707 0.0 18 0.0 0.001095626259970199 0.0 0.15010079761591727 0.0 19 0.0 0.001095626259970199 0.0 0.15557892891576824 0.0 20 0.0 0.001095626259970199 0.0 0.15886580769567885 0.0 21 0.0 0.001095626259970199 0.0 0.16653519151547025 0.0 22 0.0 0.001095626259970199 0.0 0.17091769655535105 0.0 23 0.0 0.001095626259970199 0.0 0.17968270663511263 0.0 24 0.0 0.001095626259970199 0.0 0.18954334297484443 0.0 25 0.0 0.003286878779910597 0.0 0.2004996055745464 0.0 26 0.0 0.003286878779910597 0.0 0.2213165045139802 0.0 27 0.0 0.003286878779910597 0.0 0.3473135244105531 0.0 28 0.0 0.003286878779910597 0.0 0.5215180997458148 0.0 29 0.0 0.003286878779910597 0.0 0.7570777456394074 0.0 30 0.0 0.003286878779910597 0.0 0.9400473310544307 0.0 31 0.0 0.003286878779910597 0.0 1.1591725830484705 0.0 32 0.0 0.003286878779910597 0.0 1.3772022087825402 0.0 33 0.0 0.003286878779910597 0.0 1.6927425716539575 0.0 34 0.0 0.003286878779910597 0.0 2.184678762380577 0.0 35 0.0 0.003286878779910597 0.0 2.8552020334823385 0.0 36 0.0 0.003286878779910597 0.0 3.717459900078885 0.0 37 0.0 0.003286878779910597 0.0 4.902927513366641 0.0 38 0.0 0.003286878779910597 0.0 6.040187571215707 0.0 39 0.0 0.003286878779910597 0.0 7.128144447366115 0.0 40 0.0 0.003286878779910597 0.0 8.106538697519502 0.0 41 0.0 0.003286878779910597 0.0 9.101367341572443 0.0 42 0.0 0.003286878779910597 0.0 10.031554036287142 0.0 43 0.0 0.003286878779910597 0.0 10.935445700762555 0.0 44 0.0 0.003286878779910597 0.0 11.94670873871505 0.0 45 0.0 0.003286878779910597 0.0 12.957971776667543 0.0 46 0.0 0.003286878779910597 0.0 13.976904198439827 0.0 47 0.0 0.003286878779910597 0.0 14.882987115435183 0.0 48 0.0 0.003286878779910597 0.0 15.670742396353756 0.0 49 0.0 0.003286878779910597 0.0 16.41467262687352 0.0 50 0.0 0.003286878779910597 0.0 17.193662897712333 0.0 51 0.0 0.003286878779910597 0.0 17.83679551231484 0.0 52 0.0 0.003286878779910597 0.0 18.474449995617494 0.0 53 0.0 0.003286878779910597 0.0 19.086905074940837 0.0 54 0.0 0.003286878779910597 0.0 19.722368305723553 0.0 55 0.0 0.003286878779910597 0.0 20.358927162766236 0.0 56 0.0 0.003286878779910597 0.0 21.07327548426681 0.0 57 0.0 0.003286878779910597 0.0 21.682443684810238 0.0 58 0.0 0.003286878779910597 0.0 22.338723814532386 0.0 59 0.0 0.003286878779910597 0.0 22.941318257515995 0.0 60 0.0 0.003286878779910597 0.0 23.584450872118502 0.0 61 0.0 0.003286878779910597 0.0 24.109255850644228 0.0 62 0.0 0.003286878779910597 0.0 24.620913314050313 0.002191252519940398 63 0.0 0.003286878779910597 0.0 25.26295030239285 0.002191252519940398 64 0.0 0.003286878779910597 0.0 25.843632220177053 0.002191252519940398 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTGTT 15 0.0022183284 70.0 36 TCTTAAT 25 2.8200739E-6 70.0 1 CTAAGTC 15 0.0022183284 70.0 3 TAAGTCT 15 0.0022183284 70.0 4 CCTAAGT 15 0.0022183284 70.0 2 AGTCTGG 15 0.0022183284 70.0 6 ATCCAGG 15 0.0022183284 70.0 23 TCTTTGC 15 0.0022183284 70.0 18 AGGATTA 15 0.0022183284 70.0 32 AAGGATT 15 0.0022183284 70.0 31 TGACTAC 15 0.0022183284 70.0 21 TCTTTAT 75 0.0 60.666668 1 CTATGAG 20 0.0069316486 52.499996 14 GAGTGAC 20 0.0069316486 52.499996 18 GGCAGAC 20 0.0069316486 52.499996 2 GGTTTCT 20 0.0069316486 52.499996 69 TGAGTGA 20 0.0069316486 52.499996 17 CTTGGTG 20 0.0069316486 52.499996 30 GGAAGAA 20 0.0069316486 52.499996 41 ACGATCG 20 0.0069316486 52.499996 41 >>END_MODULE