FastQCFastQC Report
Wed 25 May 2016
SRR1780261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340711
Sequences flagged as poor quality0
Sequence length50
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT207.7997206E-444.0559135
ATACTGC250.002364633435.1567968
CTTAGAC250.002364633435.1567961
TAGACAC401.8315823E-532.9594963
ACAAGGA353.236275E-431.3899943
CTTCGGA300.005773849329.2973292
TTCTACA300.005773849329.2973292
TTAGACA300.005773849329.2973292
TTCTGTT406.969742E-427.53494538
GACACAT407.072285E-427.4662485
TTATACC609.8192995E-625.6351622
CTATACA450.001406180924.4144421
TTTACAC551.6036475E-423.9705412
AAATTTG602.8793677E-421.985928
ATACCCA500.002595110321.9729984
ATTATAC703.2371638E-521.9729961
ATTTGCG755.484851E-520.52017630
TGGAAGC755.509713E-520.508135
TATACCC755.509713E-520.508133
AATTTGC654.9434823E-420.2946829
Produced by FastQC (version 0.11.5)