FastQCFastQC Report
Wed 25 May 2016
SRR1780261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340711
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT237746.977761211114405No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC66601.9547358318340178No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG57881.698800449647942No Hit
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG47931.4067640903874543Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC14590.4282221589558306No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT6500.19077752112494167Illumina PCR Primer Index 2 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACA207.851898E-443.996337
TCTTTAT1450.041.0343631
CTTTATA1550.038.325012
GTCTTCT6050.037.4569243
TCTTCTG6000.037.4023744
CGTCTTC6350.035.687342
TCTTAAT505.8376827E-835.2591551
TTTATAC2000.034.1021583
CCGTCTT6800.033.3256441
ATGCCGT32000.032.45205344
CTACAGC353.211855E-431.4305654
CCTTATA300.0056923829.3826311
GAGATAG300.00573752129.33519240
GGCACTT300.00574163929.3308875
GCCGTCT8200.027.63589940
TATGCCG38100.027.37181543
TTAATAC901.5033947E-824.4459932
TTTTATA450.001395640224.4459932
TGCCGTC9300.024.36713439
ACACATC44800.023.520366