Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780261_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 340711 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 65 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT | 23774 | 6.977761211114405 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC | 6660 | 1.9547358318340178 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG | 5788 | 1.698800449647942 | No Hit |
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG | 4793 | 1.4067640903874543 | Illumina PCR Primer Index 2 (95% over 24bp) |
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC | 1459 | 0.4282221589558306 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT | 650 | 0.19077752112494167 | Illumina PCR Primer Index 2 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGACACA | 20 | 7.851898E-4 | 43.99633 | 7 |
TCTTTAT | 145 | 0.0 | 41.034363 | 1 |
CTTTATA | 155 | 0.0 | 38.32501 | 2 |
GTCTTCT | 605 | 0.0 | 37.45692 | 43 |
TCTTCTG | 600 | 0.0 | 37.40237 | 44 |
CGTCTTC | 635 | 0.0 | 35.6873 | 42 |
TCTTAAT | 50 | 5.8376827E-8 | 35.259155 | 1 |
TTTATAC | 200 | 0.0 | 34.102158 | 3 |
CCGTCTT | 680 | 0.0 | 33.32564 | 41 |
ATGCCGT | 3200 | 0.0 | 32.452053 | 44 |
CTACAGC | 35 | 3.211855E-4 | 31.430565 | 4 |
CCTTATA | 30 | 0.00569238 | 29.382631 | 1 |
GAGATAG | 30 | 0.005737521 | 29.335192 | 40 |
GGCACTT | 30 | 0.005741639 | 29.330887 | 5 |
GCCGTCT | 820 | 0.0 | 27.635899 | 40 |
TATGCCG | 3810 | 0.0 | 27.371815 | 43 |
TTAATAC | 90 | 1.5033947E-8 | 24.445993 | 2 |
TTTTATA | 45 | 0.0013956402 | 24.445993 | 2 |
TGCCGTC | 930 | 0.0 | 24.367134 | 39 |
ACACATC | 4480 | 0.0 | 23.52036 | 6 |