FastQCFastQC Report
Wed 25 May 2016
SRR1780260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204133
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT404.581472E-543.7512
ACGATGT459.175326E-538.88888522
TAGATCT1007.2759576E-1238.539
TCGTTGT654.843969E-737.69230734
TCGTGTA501.7055686E-435.013
ATCGGTT400.002414790435.025
CGTCAGA400.002414790435.02
GGGTCCG450.00430024431.11110922
TTTGTAA450.00430024431.11110931
TGAGCGG450.00430024431.1111098
CAATACG450.00430024431.11110969
AGTGTGT450.00430024431.11110912
CATCGTT703.4769408E-530.00000232
ACCCGTA604.970113E-429.1666662
TGATATG755.565908E-528.00000219
CGACCAA755.565908E-528.00000212
GCCTTAC500.007196700727.9999987
TGCATAA500.007196700727.99999814
TCAGATG500.007196700727.9999984
AGACAAT500.007196700727.99999816