FastQCFastQC Report
Wed 25 May 2016
SRR1780260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204133
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT132626.496744769341557TruSeq Adapter, Index 5 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC35551.7415116615147967RNA PCR Primer, Index 5 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG33361.6342286646451087TruSeq Adapter, Index 5 (95% over 22bp)
CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG28881.4147639039253819TruSeq Adapter, Index 5 (96% over 29bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC7610.3727961672047146RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT4060.19888993940225244TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT3530.17292647440639192No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2100.10287410658737196No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCTT150.002222276370.030
GCTTATA150.002222276370.03
CTTTATA1100.054.0909082
CTGTAGG200.0069438952.59
GGTAACC200.0069438952.519
GATTCGG200.0069438952.56
CGTTACC305.8679824E-446.66666414
GTTACCT305.8679824E-446.66666415
GTCGTTA305.8679824E-446.66666412
CATCGTT451.6789363E-646.66666432
TCTTTAT1350.044.0740781
GTGCCTT404.581472E-543.7511
TTTATAC1450.041.0344853
ATTCATA350.00125334440.0000042
CACCGTA350.00125334440.00000441
CAATACG350.00125334440.00000469
TCGTTGT556.6721805E-638.18181634
CGTATGC28300.037.10247441
CTCGTAT28050.037.05882639
CCGTCTT28150.037.0515147
Produced by FastQC (version 0.11.5)