FastQCFastQC Report
Wed 25 May 2016
SRR1780259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649090
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG250.002356160735.18729427
CAGACTA250.002364192435.16291
CGAGAAT454.04251E-529.3589614
TTAAGCC300.00576189229.31370531
AAATTCA551.5781335E-424.03209717
TTATCAA852.3661414E-723.27673732
GTTAAGT500.002559218822.02942317
AGTTAAG500.002559218822.02942316
CTCGCTA602.8598466E-422.01242313
GATGTAT602.8628332E-422.00902724
AATTATC904.0899613E-721.99205830
AAGTTAC703.2290325E-521.9852841
GATACTA500.002595003721.976811
TACATGG602.8913395E-421.976812
GTAAGCA856.125343E-620.6984130
TAGCCCG856.1663413E-620.6824635
GACTATC755.4342057E-520.54968638
TTCGGTA755.482841E-520.52592326
ACGGTTG755.5056662E-520.5148544
ATTCAGA654.8845424E-420.33485219
Produced by FastQC (version 0.11.5)