FastQCFastQC Report
Wed 25 May 2016
SRR1780259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649090
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT236803.6481843812106183No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC64070.9870742115885317No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG62070.9562618435039826No Hit
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG47530.7322559275293102TruSeq Adapter, Index 9 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC13210.20351569119844704No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT9300.1432775115931535No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT6570.10121862915774393TruSeq Adapter, Index 9 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG6550.037.61858444
GTCTTCT6950.035.45348443
CGTCTTC7100.035.0143342
CCGTCTT7100.035.0143341
CTTTATA2200.032.0051572
ATGCCGT34200.031.84232944
GCCGTCT7950.030.99392740
TCTTTAT2300.030.653781
ATACTCG300.00574504529.33127826
TAAGCAA300.00574504529.33127812
CACGCTA804.311005E-927.49807523
TATGCCG40050.027.35599743
TTTATAC2950.026.8517823
TGCCGTC10050.024.955439
CTTATAA450.001385583424.4804481
TATTGCG450.001398087724.44273211
GTGCAAA551.5932664E-423.99832211
TCTGTAC500.0025767522.0035463
TGTATAA904.0694613E-722.0035422
TGCTAGC500.00258021121.9984611