Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780259_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 649090 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 57 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT | 23680 | 3.6481843812106183 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC | 6407 | 0.9870742115885317 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG | 6207 | 0.9562618435039826 | No Hit |
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG | 4753 | 0.7322559275293102 | TruSeq Adapter, Index 9 (96% over 26bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC | 1321 | 0.20351569119844704 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT | 930 | 0.1432775115931535 | No Hit |
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT | 657 | 0.10121862915774393 | TruSeq Adapter, Index 9 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTTCTG | 655 | 0.0 | 37.618584 | 44 |
GTCTTCT | 695 | 0.0 | 35.453484 | 43 |
CGTCTTC | 710 | 0.0 | 35.01433 | 42 |
CCGTCTT | 710 | 0.0 | 35.01433 | 41 |
CTTTATA | 220 | 0.0 | 32.005157 | 2 |
ATGCCGT | 3420 | 0.0 | 31.842329 | 44 |
GCCGTCT | 795 | 0.0 | 30.993927 | 40 |
TCTTTAT | 230 | 0.0 | 30.65378 | 1 |
ATACTCG | 30 | 0.005745045 | 29.331278 | 26 |
TAAGCAA | 30 | 0.005745045 | 29.331278 | 12 |
CACGCTA | 80 | 4.311005E-9 | 27.498075 | 23 |
TATGCCG | 4005 | 0.0 | 27.355997 | 43 |
TTTATAC | 295 | 0.0 | 26.851782 | 3 |
TGCCGTC | 1005 | 0.0 | 24.9554 | 39 |
CTTATAA | 45 | 0.0013855834 | 24.480448 | 1 |
TATTGCG | 45 | 0.0013980877 | 24.442732 | 11 |
GTGCAAA | 55 | 1.5932664E-4 | 23.998322 | 11 |
TCTGTAC | 50 | 0.00257675 | 22.003546 | 3 |
TGTATAA | 90 | 4.0694613E-7 | 22.003542 | 2 |
TGCTAGC | 50 | 0.002580211 | 21.99846 | 11 |