FastQCFastQC Report
Wed 25 May 2016
SRR1780258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102626
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCATT252.380753E-455.99999611
CTAAGGC200.006934095652.513
TAAGGCG200.006934095652.514
GAATTGA200.006934095652.526
ATACGGT200.006934095652.542
AGTACCC200.006934095652.556
ACGAATT200.006934095652.524
TTATAAA350.001250432140.02
TCGATCT400.00240921335.039
AGTGATT400.00240921335.065
AACGAAT400.00240921335.027
CAGTGAT400.00240921335.064
CAGCAAG450.00429037231.1111131
CTTATAA450.00429037231.1111131
GTATATC450.00429037231.11111338
AACCTAG450.00429037231.11111330
ATCCAAC500.007180282427.9999981
GAACGAA500.007180282427.99999826
TTCTACA808.595387E-526.252
TATAAAC700.001218954925.03
Produced by FastQC (version 0.11.5)