##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780258_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 102626 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.287773078946856 34.0 27.0 34.0 12.0 34.0 2 29.433028667199345 34.0 27.0 34.0 12.0 34.0 3 29.24039717030772 34.0 27.0 34.0 11.0 34.0 4 29.238545787617173 34.0 27.0 34.0 11.0 34.0 5 29.111511702687427 34.0 27.0 34.0 11.0 34.0 6 31.876717401048467 37.0 31.0 38.0 11.0 38.0 7 32.21963245181533 37.0 31.0 38.0 12.0 38.0 8 32.25565646132559 37.0 31.0 38.0 11.0 38.0 9 32.39769649016819 38.0 31.0 38.0 12.0 38.0 10-11 32.09257400658703 37.5 31.0 38.0 11.0 38.0 12-13 32.17039541636622 38.0 31.0 38.0 11.0 38.0 14-15 32.026635550445306 37.5 31.0 38.0 11.0 38.0 16-17 32.03812873930583 38.0 31.0 38.0 11.0 38.0 18-19 32.07602849180519 38.0 31.0 38.0 11.0 38.0 20-21 32.05586303665738 38.0 31.0 38.0 11.0 38.0 22-23 32.08122697951786 38.0 31.0 38.0 11.0 38.0 24-25 32.06121743028083 38.0 31.0 38.0 11.0 38.0 26-27 32.00390251982928 38.0 31.0 38.0 11.0 38.0 28-29 32.053056730263286 38.0 31.0 38.0 11.0 38.0 30-31 32.07449379299592 38.0 31.0 38.0 11.0 38.0 32-33 32.1185031083741 38.0 31.0 38.0 11.0 38.0 34-35 32.09629138814725 38.0 31.0 38.0 11.0 38.0 36-37 32.00432638902423 38.0 31.0 38.0 11.0 38.0 38-39 31.95058757040126 38.0 31.0 38.0 11.0 38.0 40-41 32.041646366417865 38.0 31.0 38.0 11.0 38.0 42-43 32.092895562528014 38.0 31.0 38.0 11.0 38.0 44-45 32.20355465476585 38.0 31.0 38.0 11.0 38.0 46-47 32.08864225439947 38.0 31.0 38.0 11.0 38.0 48-49 32.01350047746185 38.0 31.0 38.0 11.0 38.0 50-51 31.9585436439109 38.0 30.5 38.0 11.0 38.0 52-53 32.03743690682673 38.0 31.0 38.0 11.0 38.0 54-55 32.00849687213766 38.0 31.0 38.0 11.0 38.0 56-57 31.96627560267379 38.0 30.5 38.0 11.0 38.0 58-59 31.975756630873267 38.0 30.5 38.0 11.0 38.0 60-61 32.00562722896732 38.0 30.5 38.0 11.0 38.0 62-63 31.86940931148052 37.5 29.0 38.0 11.0 38.0 64-65 31.81058406251827 37.0 28.5 38.0 11.0 38.0 66-67 31.74268216631263 37.0 28.0 38.0 11.0 38.0 68-69 31.640412760898798 37.0 27.0 38.0 11.0 38.0 70-71 31.58083234268119 37.0 27.0 38.0 11.0 38.0 72-73 31.522562508526104 37.0 27.0 38.0 11.0 38.0 74-75 31.487902675735192 37.0 27.0 38.0 11.0 38.0 76 29.7744333794555 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02393466175103498 1101 2 -0.09778776899101871 1101 3 -0.19661230591079715 1101 4 0.5351145599871394 1101 5 -0.6050045855370279 1101 6 -0.3832530040678428 1101 7 -0.7687799644485516 1101 8 -0.8664529243126147 1101 9 -0.8379518760609699 1101 10-11 -1.3604612920298464 1101 12-13 -0.6046178479621673 1101 14-15 -0.7043451413570025 1101 16-17 -0.6042680258795912 1101 18-19 -0.7659529284654241 1101 20-21 -0.2698097196501408 1101 22-23 -0.6782701556993977 1101 24-25 -0.8611423482894374 1101 26-27 -0.7278329491301996 1101 28-29 -1.2613755739661912 1101 30-31 -1.2822513279920429 1101 32-33 -0.9312337836266593 1101 34-35 -0.6468389700908475 1101 36-37 -0.3813343612021036 1101 38-39 -0.576745173970707 1101 40-41 -0.4439029682973974 1101 42-43 -0.9697880056496402 1101 44-45 -0.9888771631834885 1101 46-47 -1.1033555941358166 1101 48-49 -0.7201017021038005 1101 50-51 -0.6491242083044995 1101 52-53 -0.6590046580400646 1101 54-55 -0.4924541982539026 1101 56-57 -0.6637312712308656 1101 58-59 -0.9603040125617852 1101 60-61 -1.2019862343706187 1101 62-63 -1.5556275640151611 1101 64-65 -0.8849551377804481 1101 66-67 -1.0080780231575304 1101 68-69 -0.8878650990429193 1101 70-71 -0.5920625700085829 1101 72-73 -0.4507984644323031 1101 74-75 -0.8224244264862328 1101 76 -1.3815818491783496 1102 1 0.9636551668835693 1102 2 0.5926489363710594 1102 3 1.0886005188542676 1102 4 0.5257723819882472 1102 5 1.5357361552037112 1102 6 1.1544473828862785 1102 7 1.0206340764580304 1102 8 1.6672397235591419 1102 9 1.4795163384221723 1102 10-11 0.9954950374339475 1102 12-13 1.4969437883009604 1102 14-15 1.1880677939663826 1102 16-17 0.6724456280728681 1102 18-19 0.5849177183007441 1102 20-21 0.43880000948198017 1102 22-23 1.0205689819457078 1102 24-25 0.9464723559670531 1102 26-27 0.5738392454524472 1102 28-29 0.6220130385269016 1102 30-31 1.1543158361870596 1102 32-33 1.2098662716525013 1102 34-35 0.7277740757908546 1102 36-37 1.3346413159676054 1102 38-39 1.2574588061287955 1102 40-41 1.05566861821449 1102 42-43 1.1349383625095655 1102 44-45 0.8377259324494588 1102 46-47 0.498952310784027 1102 48-49 0.8839751359282637 1102 50-51 0.6822190752775867 1102 52-53 1.014917398344675 1102 54-55 0.6943617221441087 1102 56-57 1.1899724324728354 1102 58-59 0.8878303157964247 1102 60-61 1.121465949156189 1102 62-63 0.470063425481797 1102 64-65 0.9540304896380185 1102 66-67 1.0734863770635883 1102 68-69 1.210656183433585 1102 70-71 1.7061132177194445 1102 72-73 0.9707742276627798 1102 74-75 0.8168375967310162 1102 76 0.6008946571787526 1103 1 0.24197334271064364 1103 2 0.27664788193584045 1103 3 0.23772401957918987 1103 4 0.15470344828586136 1103 5 -0.20820743251211837 1103 6 -0.18160112113375604 1103 7 0.5850337074056995 1103 8 0.07351361310705329 1103 9 0.164385193041916 1103 10-11 0.11802040978138706 1103 12-13 0.012786677453455297 1103 14-15 -0.23482663070740628 1103 16-17 0.47716422567402716 1103 18-19 0.029520328027906828 1103 20-21 0.22524392670882065 1103 22-23 -0.5102534775244365 1103 24-25 0.3029637227787063 1103 26-27 -0.06080554168344321 1103 28-29 -0.06619046747020008 1103 30-31 -0.44799733107272743 1103 32-33 -0.08646297574436801 1103 34-35 0.4899791634407862 1103 36-37 0.04673436987560464 1103 38-39 -0.24212601000406409 1103 40-41 -0.08003060413954088 1103 42-43 0.14958842182154086 1103 44-45 -0.46418523138341783 1103 46-47 0.7002044526055684 1103 48-49 0.49664153898612184 1103 50-51 0.12670306104271845 1103 52-53 0.15232348770805615 1103 54-55 0.17164523341338622 1103 56-57 0.4029947074168234 1103 58-59 0.0894317392839703 1103 60-61 0.5192186840975381 1103 62-63 0.05411509280748916 1103 64-65 0.34696577367650505 1103 66-67 0.4346106686149227 1103 68-69 0.1402660424028781 1103 70-71 -0.39438104878905733 1103 72-73 0.23553632071668673 1103 74-75 0.12207682172113365 1103 76 0.355949356005695 1104 1 -0.12403897887509174 1104 2 -0.2598002870459055 1104 3 -0.5333827547419112 1104 4 0.10366963407904706 1104 5 -0.7322447289062026 1104 6 0.636406761619849 1104 7 0.14261735005420206 1104 8 -0.5365834544575883 1104 9 -0.221678404708058 1104 10-11 0.1712417518903422 1104 12-13 0.24688405114445544 1104 14-15 -0.030570198865163434 1104 16-17 0.12573117168099301 1104 18-19 -0.16275253259531297 1104 20-21 -0.689933562799979 1104 22-23 -0.5867786883051878 1104 24-25 -0.22831856398515527 1104 26-27 -0.5737712682871035 1104 28-29 0.0014634566869986543 1104 30-31 -0.022207461303977993 1104 32-33 0.2525783385581164 1104 34-35 0.1445602167705502 1104 36-37 -0.08572905440276912 1104 38-39 -0.08286377451636895 1104 40-41 -0.1451079648201592 1104 42-43 -0.1498286625800418 1104 44-45 0.4242253350778924 1104 46-47 0.25928683886048987 1104 48-49 -0.208259033725259 1104 50-51 -0.42853361289445147 1104 52-53 -0.06035141885962503 1104 54-55 0.004576775907544572 1104 56-57 0.5002472233927904 1104 58-59 -0.27399764119282466 1104 60-61 -0.10583393136949582 1104 62-63 -0.5649425482338515 1104 64-65 -0.17655493449579396 1104 66-67 0.02910166977565609 1104 68-69 0.08992739012415285 1104 70-71 0.09804870159041101 1104 72-73 0.3181457927762814 1104 74-75 -0.0722305036274058 1104 76 -0.07341836552136627 1105 1 0.3388740221024271 1105 2 1.0257129094350326 1105 3 0.5562099464636958 1105 4 0.6248296210454853 1105 5 0.9277226871902435 1105 6 0.6062992347381275 1105 7 1.103933739757693 1105 8 0.5511454474648616 1105 9 0.7202570791629128 1105 10-11 1.029097731036643 1105 12-13 -0.0710696797125081 1105 14-15 0.4268556727542574 1105 16-17 0.500257075884317 1105 18-19 0.5843116576946841 1105 20-21 0.7470491677311415 1105 22-23 1.373195244571967 1105 24-25 0.6859336354283343 1105 26-27 0.7106405922537888 1105 28-29 1.0142689307827908 1105 30-31 0.6406794725506941 1105 32-33 0.4927282245144511 1105 34-35 0.5859222239390007 1105 36-37 0.7581093294356158 1105 38-39 1.1826103212803076 1105 40-41 0.5528066653525343 1105 42-43 0.5200898776610785 1105 44-45 1.2634498381733579 1105 46-47 1.1600297518614724 1105 48-49 0.8911805231336523 1105 50-51 0.9749463480048632 1105 52-53 0.8868365902638651 1105 54-55 0.8176950554774436 1105 56-57 0.6280869105873137 1105 58-59 0.6960121339782397 1105 60-61 0.7749676326578765 1105 62-63 0.95356510898349 1105 64-65 0.849922745530268 1105 66-67 1.1331160066932213 1105 68-69 1.085235308012713 1105 70-71 0.5662142278204563 1105 72-73 0.3887876956762497 1105 74-75 0.7361305260239455 1105 76 0.18729196357605815 1106 1 0.5804755974224136 1106 2 0.3006981408101552 1106 3 0.3017038749179122 1106 4 1.0518988694865747 1106 5 0.6278474000225387 1106 6 0.7478154802254942 1106 7 0.5241372185893312 1106 8 0.8047982206844253 1106 9 0.9209791008235584 1106 10-11 0.7035709834160286 1106 12-13 0.7533149609176206 1106 14-15 1.0538921020289536 1106 16-17 0.6609200230454597 1106 18-19 0.44183709254863857 1106 20-21 0.6307708612001264 1106 22-23 0.3644719101446654 1106 24-25 0.7273120404168552 1106 26-27 1.1296822725951046 1106 28-29 0.9130546216262445 1106 30-31 1.1461930925047525 1106 32-33 0.7968765671465476 1106 34-35 1.2508319712314275 1106 36-37 1.1354903599574406 1106 38-39 0.6996369048050894 1106 40-41 0.8303911747060013 1106 42-43 0.5067193492725011 1106 44-45 1.4265479674808788 1106 46-47 0.5683198732923884 1106 48-49 0.9322569917913448 1106 50-51 1.1381757539779542 1106 52-53 1.1318447950554678 1106 54-55 0.9831102179323139 1106 56-57 0.842586418299824 1106 58-59 1.191259918684505 1106 60-61 1.54528597857189 1106 62-63 0.610309447677281 1106 64-65 0.9494042027012632 1106 66-67 0.7296503027220993 1106 68-69 0.7879592988233455 1106 70-71 1.1192500007118191 1106 72-73 1.3748723691255407 1106 74-75 1.4039414876255272 1106 76 0.8303638375504612 1107 1 0.9189717738130803 1107 2 0.5860778491548118 1107 3 0.43435918074196067 1107 4 0.6699778778066445 1107 5 0.3071817943912869 1107 6 -0.0661021989536259 1107 7 0.5440510418104765 1107 8 0.7358179792341701 1107 9 1.1905079752202568 1107 10-11 0.45798010314481985 1107 12-13 0.8167765242627283 1107 14-15 0.6450570412782071 1107 16-17 1.0863046483835 1107 18-19 1.0594696896269085 1107 20-21 1.1989553260889139 1107 22-23 1.07451163427546 1107 24-25 0.952685020244239 1107 26-27 0.6986301654046514 1107 28-29 0.856302166364415 1107 30-31 0.7394412842157365 1107 32-33 0.95329518443625 1107 34-35 1.0710835142615807 1107 36-37 0.996708221582896 1107 38-39 1.237924755949578 1107 40-41 1.4749637197676506 1107 42-43 1.3340423051618941 1107 44-45 1.1751213924255595 1107 46-47 0.8471004589321751 1107 48-49 1.3060778838374567 1107 50-51 1.5410004372847652 1107 52-53 1.1421575417138534 1107 54-55 1.0601323314631017 1107 56-57 1.5808923846831178 1107 58-59 0.9249270899899749 1107 60-61 1.3081445632541602 1107 62-63 1.1196452653862181 1107 64-65 1.253552592385926 1107 66-67 1.3678113471950084 1107 68-69 1.2332965657513881 1107 70-71 1.3131024650312675 1107 72-73 1.6210131762888267 1107 74-75 1.6571601773761806 1107 76 1.600416759926663 1108 1 0.05924733700045692 1108 2 -0.11887056792410888 1108 3 0.14552207919788174 1108 4 -0.19566121891303467 1108 5 0.3588737414591705 1108 6 0.1796832651563811 1108 7 0.30021441725509845 1108 8 0.36646518476178613 1108 9 0.303527041140093 1108 10-11 0.4683580144794348 1108 12-13 -0.17801748475917378 1108 14-15 0.03280797140617153 1108 16-17 0.3480828622652403 1108 18-19 -0.1103928013443749 1108 20-21 -0.2538841195139341 1108 22-23 -0.09639341771630683 1108 24-25 -0.3207998592138992 1108 26-27 -0.0724288857966755 1108 28-29 0.018900800813902663 1108 30-31 -0.06146363149354528 1108 32-33 -0.1308251802556839 1108 34-35 -0.13871655933095894 1108 36-37 -0.24838220184679116 1108 38-39 0.038890998777716845 1108 40-41 -0.26029392227276915 1108 42-43 -0.07222260073505282 1108 44-45 -0.49346685729846484 1108 46-47 -0.6710659964966332 1108 48-49 -0.028093586786845037 1108 50-51 -0.22386457491252187 1108 52-53 -0.24511639971193588 1108 54-55 -0.047019773419897604 1108 56-57 -0.0068871647669865865 1108 58-59 -0.15418489332905239 1108 60-61 -0.27738632517137063 1108 62-63 0.2177890979222994 1108 64-65 -0.466952470508442 1108 66-67 -0.2639942594664788 1108 68-69 0.1767665904185236 1108 70-71 0.1626193436172052 1108 72-73 0.05086858057082111 1108 74-75 -0.48402847846965713 1108 76 -0.4925006392583704 1109 1 0.8006922039206081 1109 2 0.09805884065736947 1109 3 0.6947594868109874 1109 4 1.4443325151551214 1109 5 0.8575422309423715 1109 6 1.217535189794308 1109 7 0.9182204268533667 1109 8 1.0490957368758274 1109 9 0.9472233712977385 1109 10-11 1.0578753953199254 1109 12-13 1.0771768303317586 1109 14-15 1.062599629172368 1109 16-17 0.49083930264631803 1109 18-19 1.0257416883382007 1109 20-21 0.9973726270433545 1109 22-23 1.1865490069144968 1109 24-25 1.0366929136033463 1109 26-27 1.0540930948074134 1109 28-29 0.8518923560466618 1109 30-31 0.7601210796327535 1109 32-33 0.7567867876741374 1109 34-35 1.0083192276843178 1109 36-37 0.6545955398544088 1109 38-39 0.9958483192374104 1109 40-41 0.638710649009326 1109 42-43 0.745987051648136 1109 44-45 0.579132507563898 1109 46-47 0.8308707460732521 1109 48-49 0.7188482112507764 1109 50-51 0.9080617719068016 1109 52-53 0.6940684595182098 1109 54-55 0.7511032951778134 1109 56-57 0.9345833355107089 1109 58-59 1.3393284431372443 1109 60-61 1.0033843844454111 1109 62-63 1.183255269010722 1109 64-65 0.7011382715772534 1109 66-67 0.7474980291651292 1109 68-69 0.4331072862217695 1109 70-71 0.8030545410652614 1109 72-73 0.8884812605383487 1109 74-75 1.037873801475083 1109 76 0.6834581195174962 1110 1 0.1557646676887252 1110 2 0.053196440396824585 1110 3 0.19117700838727814 1110 4 -0.5304272959505631 1110 5 0.366009907216597 1110 6 0.8005021332888553 1110 7 -0.12542067394454648 1110 8 -0.15852517177097525 1110 9 0.28837302119189445 1110 10-11 0.7334982580458629 1110 12-13 0.09171029393703378 1110 14-15 0.42719822874898483 1110 16-17 0.36077090987641114 1110 18-19 0.28817858786595707 1110 20-21 0.08815588709872912 1110 22-23 -0.027015559117089083 1110 24-25 0.46575576981568645 1110 26-27 0.28595679778739225 1110 28-29 -0.3680433221816344 1110 30-31 0.39163467676677044 1110 32-33 0.34553454862512467 1110 34-35 0.47845845582306623 1110 36-37 0.3279182885924037 1110 38-39 0.024487791636040868 1110 40-41 0.17217425428533772 1110 42-43 -0.08716308676579843 1110 44-45 0.7637726313222402 1110 46-47 -0.09794785024656605 1110 48-49 0.009619258311516887 1110 50-51 0.2561321187558576 1110 52-53 0.1266323741900841 1110 54-55 0.2095791134484557 1110 56-57 0.633370178044494 1110 58-59 0.20268538826018911 1110 60-61 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0.22520333295754114 2116 22-23 0.47584992829128936 2116 24-25 -0.09266297708462545 2116 26-27 0.06825552484971098 2116 28-29 0.013197378760665401 2116 30-31 -0.09948644518176053 2116 32-33 -0.16694685325948555 2116 34-35 -0.17293709165034699 2116 36-37 -0.4175837227593391 2116 38-39 -0.26529151072798385 2116 40-41 -0.459913694135885 2116 42-43 -0.577926022788283 2116 44-45 -0.0029725607205222104 2116 46-47 0.026652634682072573 2116 48-49 -0.09463351074122173 2116 50-51 -0.2770965140760211 2116 52-53 0.24157561549719375 2116 54-55 -0.005194678362862248 2116 56-57 -0.39281872370234794 2116 58-59 -0.06673242184616512 2116 60-61 -0.2830897472246008 2116 62-63 -0.008446643522560748 2116 64-65 -0.7312110256415245 2116 66-67 -0.028252814599557752 2116 68-69 -0.5798874019769187 2116 70-71 -0.010384458664162821 2116 72-73 -0.5384850315964798 2116 74-75 -0.4821273377852471 2116 76 -0.4316641373572274 2117 1 -0.39248961426120843 2117 2 -0.6085295148073904 2117 3 -0.4315173262635774 2117 4 -0.8169885607726961 2117 5 -0.49515610068854343 2117 6 -0.6194583410194454 2117 7 -0.5463692905453392 2117 8 -0.5262787949593779 2117 9 -1.1615611026552735 2117 10-11 -0.8177204507815397 2117 12-13 -1.0888727100155364 2117 14-15 -0.471023115124531 2117 16-17 -0.503606560479323 2117 18-19 -0.5347792513962268 2117 20-21 -0.7627993171173486 2117 22-23 -0.30089566451893646 2117 24-25 -0.5623239403292359 2117 26-27 -0.42632910471590435 2117 28-29 -0.5531553116414472 2117 30-31 -0.31159325472203037 2117 32-33 -0.757726320940094 2117 34-35 -0.06839595787917574 2117 36-37 -0.5257543069280217 2117 38-39 -0.6978442241742364 2117 40-41 -0.5718145467686782 2117 42-43 -1.076046485975283 2117 44-45 -0.5357168284933067 2117 46-47 -0.5095914602597418 2117 48-49 -0.20571341626028783 2117 50-51 -0.20565971260119653 2117 52-53 -0.5526331067058301 2117 54-55 -0.7779867627043799 2117 56-57 -0.7072918772914747 2117 58-59 -0.5398211993550923 2117 60-61 -0.4601838824936415 2117 62-63 -0.4812833758650008 2117 64-65 -0.6351530120454889 2117 66-67 -0.3283233872461757 2117 68-69 -0.8109768131994066 2117 70-71 -0.5428766812704531 2117 72-73 -1.6066668497782999 2117 74-75 -0.7758391709457513 2117 76 -0.8419504606663644 2118 1 -0.1300770268486211 2118 2 -0.5465248528027296 2118 3 -0.11980403955029217 2118 4 -0.9030025467866842 2118 5 -0.8665430470754885 2118 6 -0.11293153449264182 2118 7 -0.690192134368182 2118 8 -0.25640700008758444 2118 9 -0.9874352285293995 2118 10-11 -2.042895275956365 2118 12-13 -1.0053354139782407 2118 14-15 -0.8266757957772128 2118 16-17 -0.6479247422975014 2118 18-19 -0.2503386919556654 2118 20-21 -0.5421116714297014 2118 22-23 -0.750371188994464 2118 24-25 -0.6293111198164176 2118 26-27 -0.9290097573965603 2118 28-29 -0.5827590412451791 2118 30-31 -0.6129918561206296 2118 32-33 -0.6288801670939392 2118 34-35 -0.40292392990714987 2118 36-37 -0.6053871740608905 2118 38-39 -0.9880540143840264 2118 40-41 -0.6036618661159991 2118 42-43 -0.6990359964647936 2118 44-45 -0.8378438681203448 2118 46-47 -1.1860191991874771 2118 48-49 -0.30819010623697807 2118 50-51 -1.1639347708762529 2118 52-53 -0.892988584561305 2118 54-55 -0.7607664829840992 2118 56-57 -0.9425774250770189 2118 58-59 -0.3544773765112659 2118 60-61 -0.3994029967127517 2118 62-63 -1.066190135771759 2118 64-65 -0.8180492824417591 2118 66-67 -1.3257301471529352 2118 68-69 -1.0586458108684056 2118 70-71 -0.6575619959557706 2118 72-73 -0.9596388777503257 2118 74-75 -1.0970512921578752 2118 76 -0.7934073371232415 2119 1 0.5780644666943324 2119 2 0.4943791337216936 2119 3 -0.11988826189728741 2119 4 0.5877891432750815 2119 5 0.5154333706673526 2119 6 0.2719926653950644 2119 7 0.18897687180812284 2119 8 0.3207406697608164 2119 9 0.4253665682140024 2119 10-11 0.8542271271879329 2119 12-13 0.859852682596717 2119 14-15 0.8991383535770865 2119 16-17 0.48382708252001905 2119 18-19 1.0577887645294552 2119 20-21 0.5822847350834977 2119 22-23 0.08654627310548335 2119 24-25 0.6942149891111526 2119 26-27 0.7022928815703153 2119 28-29 0.7114155797542594 2119 30-31 0.3076005077199042 2119 32-33 0.45486491993581524 2119 34-35 0.5591339027711228 2119 36-37 0.7790316917448372 2119 38-39 0.5334928693891356 2119 40-41 0.5986261743107306 2119 42-43 0.4127972432881464 2119 44-45 0.5423217691620863 2119 46-47 0.412591132087087 2119 48-49 0.6532641329544866 2119 50-51 0.5037519219929152 2119 52-53 0.6434656546301589 2119 54-55 0.5360892160613844 2119 56-57 0.2048818674552635 2119 58-59 0.35899819900810215 2119 60-61 0.5388760599969586 2119 62-63 0.6608312005561459 2119 64-65 0.3197104031281448 2119 66-67 0.24034228406548408 2119 68-69 0.03659562652632076 2119 70-71 0.38064687546478027 2119 72-73 0.7607850413962183 2119 74-75 0.733423160396871 2119 76 0.7965421294685591 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 34.0 13 431.0 14 1379.0 15 2172.0 16 2968.0 17 3324.0 18 3462.0 19 3039.0 20 2374.0 21 1733.0 22 1306.0 23 980.0 24 843.0 25 825.0 26 858.0 27 972.0 28 1108.0 29 1319.0 30 1583.0 31 1976.0 32 2576.0 33 3228.0 34 4478.0 35 6718.0 36 12239.0 37 40701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.410597704285465 18.123087716563056 14.068559624266754 30.397754954884725 2 21.400035078829927 18.555726618985442 30.836240328961473 29.20799797322316 3 19.377155886422546 21.925243115779626 25.942743554265 32.75485744353283 4 16.043692631496892 17.987644456570457 25.926178551244323 40.04248436068833 5 18.770097246311853 29.759515132617466 28.24235573831193 23.228031882758753 6 31.344883362890496 29.950499873326446 15.351860152398029 23.35275661138503 7 29.355134176524466 25.532516126517645 17.208114902656245 27.90423479430164 8 25.475025821916475 30.404575838481477 17.09995517705065 27.0204431625514 9 23.004891547950812 18.116266832966303 17.760606473992947 41.11823514508994 10-11 27.522265312883675 21.899421199306218 21.491142595443648 29.08717089236646 12-13 25.56272289673182 18.97764699004151 22.213669050727887 33.24596106249878 14-15 25.427766842710426 20.22927913004502 20.13573558357531 34.20721844366925 16-17 26.657961919981293 21.48042406407733 20.39444195428059 31.467172061660786 18-19 25.830686180889835 20.22927913004502 21.21197357394812 32.72806111511702 20-21 25.97636076627755 20.864108510513905 21.78638941398866 31.37314130921989 22-23 27.32494689454914 20.54693742326506 20.54011653966831 31.587999142517493 24-25 26.285736557987256 21.544735252275252 19.984701732504433 32.184826457233065 26-27 25.661138502913495 21.949603414339446 21.22707695905521 31.162181123691852 28-29 26.229220665328473 21.005398242160854 22.51330072301366 30.25208036949701 30-31 25.95066528299497 20.361898358595084 22.494896492587124 31.192539865822823 32-33 25.302554908112956 21.375674780270106 22.460682478124454 30.86108783349249 34-35 25.53349053846004 20.790053202892057 22.954222126946387 30.72223413170152 36-37 25.402432132208215 19.92867304581685 23.150078927367336 31.518815894607606 38-39 24.850914972813907 19.740611540935046 25.407304191920176 30.00116929433087 40-41 24.33837429111531 19.65681211388927 25.08672266287296 30.918090932122468 42-43 23.11158965564282 19.774228752947597 25.298657260343383 31.815524331066204 44-45 22.469939391577185 19.639272698926195 26.603394851207295 31.28739305828932 46-47 22.864576228246253 20.732075692319686 26.229707871299674 30.17364020813439 48-49 22.629742950129597 20.935240582308577 25.759066903123966 30.67594956443786 50-51 22.65897530840138 21.77274764679516 24.77637245922086 30.791904585582603 52-53 21.611482470329157 22.00417048311344 25.074542513593045 31.309804532964357 54-55 21.685537777951005 21.36057139516302 24.7710131935377 32.18287763334827 56-57 21.9422953247715 21.939372088944324 24.443123574922534 31.675209011361645 58-59 22.29941730165845 21.699179545144506 23.935454952935903 32.06594820026114 60-61 21.955937091965 20.809054235768716 24.61608169469725 32.61892697756903 62-63 21.983707832323194 21.01952721532555 24.276986338744567 32.719778613606685 64-65 22.58345838286594 21.121353263305593 23.30939527994855 32.98579307387992 66-67 22.311597450938358 22.290647594176917 21.9291407635492 33.468614191335526 68-69 22.394909672012943 22.557636466392534 22.190770370081655 32.856683491512875 70-71 23.364449554693742 21.86044472161051 22.33936819129655 32.435737532399195 72-73 23.110615243700426 21.206127102293763 23.509636934110265 32.17362071989554 74-75 23.343499697932298 21.11550679165124 23.96955937091965 31.571434139496812 76 23.20951805585329 19.902363923372246 24.105002630912246 32.78311538986222 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 2.5 15 4.0 16 6.5 17 9.0 18 11.5 19 16.0 20 23.0 21 28.0 22 36.5 23 51.5 24 75.0 25 92.0 26 130.5 27 191.0 28 282.5 29 352.0 30 399.5 31 561.5 32 786.5 33 897.0 34 1020.5 35 1349.5 36 1692.5 37 1830.0 38 2061.5 39 2559.0 40 3047.5 41 3374.5 42 3479.0 43 3669.0 44 3991.0 45 4041.0 46 3959.0 47 4012.5 48 3925.5 49 3662.5 50 3540.0 51 3266.5 52 2802.0 53 2547.5 54 2484.0 55 2410.5 56 2224.5 57 2064.0 58 2016.0 59 2175.0 60 2423.5 61 2691.5 62 2870.0 63 2950.5 64 3097.5 65 3264.0 66 3415.5 67 3467.0 68 3483.0 69 3520.5 70 3442.5 71 3343.0 72 3305.0 73 3129.5 74 2799.5 75 2607.0 76 2315.5 77 1697.5 78 1163.0 79 955.0 80 726.5 81 371.5 82 169.0 83 93.0 84 67.5 85 30.5 86 12.0 87 5.0 88 8.0 89 8.5 90 5.5 91 8.5 92 12.0 93 8.5 94 3.0 95 2.5 96 4.0 97 6.0 98 5.0 99 10.5 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 4.8720597119638297E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 102626.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.77323485276635 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29835184229819 56.197259953617994 2 13.360395340628308 19.17837585017442 3 4.617285291482256 9.94192504823339 4 1.7703440223736728 5.082532691520667 5 0.7697738195443807 2.7624578566834916 6 0.4045724836406093 1.7422485529982656 7 0.24029976377311357 1.207296396624637 8 0.19142523554807353 1.09913667101904 9 0.10453718537022454 0.6752674760781868 >10 0.24301501534117137 2.1134995030499093 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.744119423927659E-4 0.0 2 0.0 0.0 0.0 9.744119423927659E-4 0.0 3 0.0 0.0 0.0 0.002923235827178298 0.0 4 0.0 0.0 0.0 0.028257946329390212 0.0 5 0.0 0.0 0.0 0.035078829926139574 0.0 6 0.0 0.0 0.0 0.035078829926139574 0.0 7 0.0 0.0 0.0 0.035078829926139574 0.0 8 0.0 0.0 0.0 0.038002065753317875 0.0 9 0.0 0.0 0.0 0.04092530158049617 0.0 10 0.0 0.0 0.0 0.0428741254652817 0.0 11 0.0 0.0 0.0 0.0428741254652817 0.0 12 0.0 0.0 0.0 0.04482294935006723 0.0 13 0.0 0.0 0.0 0.046771773234852765 0.0 14 0.0 0.0 0.0 0.0487205971196383 0.0 15 0.0 0.0 0.0 0.0487205971196383 0.0 16 0.0 0.0 0.0 0.049695009062031066 0.0 17 0.0 0.0 0.0 0.049695009062031066 0.0 18 0.0 0.0 0.0 0.059439128485958725 0.0 19 0.0 0.0 0.0 0.07502971956424298 0.0 20 0.0 0.0 0.0 0.08282501510338511 0.0 21 0.0 0.0 0.0 0.08672266287295617 0.0 22 0.0 0.0 0.0 0.09062031064252724 0.0 23 0.0 0.0 0.0 0.09549237035449107 0.0 24 0.0 0.0 0.0 0.10815972560559703 0.0 25 0.0 0.0 0.0 0.11303178531756085 0.0 26 0.0 0.0 0.0 0.13544325999259446 0.0 27 0.0 0.0 0.0 0.23093563034708553 0.0 28 0.0 0.0 0.0 0.3351977081831115 0.0 29 0.0 0.0 0.0 0.4433574337887085 0.0 30 0.0 0.0 0.0 0.5388498041431996 0.0 31 0.0 0.0 0.0 0.6869604193869 0.0 32 0.0 0.0 0.0 0.8535848615360629 0.0 33 0.0 0.0 0.0 1.0669810769200787 0.0 34 0.0 0.0 0.0 1.4041276089879757 0.0 35 0.0 0.0 0.0 1.8309200397560073 0.0 36 0.0 0.0 0.0 2.3824371991503126 0.0 37 0.0 0.0 0.0 3.1444273381014556 0.0 38 0.0 0.0 0.0 3.8235924619492137 0.0 39 0.0 0.0 0.0 4.4832693469491165 0.0 40 0.0 0.0 0.0 5.1429462319490185 0.0 41 0.0 0.0 0.0 5.869857540974023 0.0 42 0.0 0.0 0.0 6.625026796328416 0.0 43 0.0 0.0 0.0 7.365579872546918 0.0 44 0.0 0.0 0.0 8.1762906086177 0.0 45 0.0 0.0 0.0 8.894432210161169 0.0 46 0.0 0.0 0.0 9.658371172997096 0.0 47 0.0 0.0 0.0 10.369691890943816 0.0 48 0.0 9.744119423927659E-4 0.0 11.022547892346969 0.0 49 0.0 9.744119423927659E-4 0.0 11.652992419075089 0.0 50 0.0 9.744119423927659E-4 0.0 12.228869877029213 0.0 51 0.0 9.744119423927659E-4 0.0 12.852493520160584 0.0 52 0.0 9.744119423927659E-4 0.0 13.44980804084735 0.0 53 0.0 9.744119423927659E-4 0.0 14.027634322686259 0.0 54 0.0 9.744119423927659E-4 0.0 14.631769726969774 0.0 55 0.0 9.744119423927659E-4 0.0 15.237853955138075 0.0 56 0.0 9.744119423927659E-4 0.0 15.762087580145382 0.0 57 0.0 9.744119423927659E-4 0.0 16.315553563424473 0.0 58 0.0 9.744119423927659E-4 0.0 16.833940716777423 0.0 59 0.0 9.744119423927659E-4 0.0 17.326018747685772 0.0 60 0.0 9.744119423927659E-4 0.0 17.86389413988658 0.0 61 0.0 9.744119423927659E-4 0.0 18.365716290218852 0.0 62 0.0 9.744119423927659E-4 0.0 18.887026679398982 0.0 63 0.0 9.744119423927659E-4 0.0 19.41710677606065 0.0 64 0.0 9.744119423927659E-4 0.0 19.9890865862452 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCATT 25 2.380753E-4 55.999996 11 CTAAGGC 20 0.0069340956 52.5 13 TAAGGCG 20 0.0069340956 52.5 14 GAATTGA 20 0.0069340956 52.5 26 ATACGGT 20 0.0069340956 52.5 42 AGTACCC 20 0.0069340956 52.5 56 ACGAATT 20 0.0069340956 52.5 24 TTATAAA 35 0.0012504321 40.0 2 TCGATCT 40 0.002409213 35.0 39 AGTGATT 40 0.002409213 35.0 65 AACGAAT 40 0.002409213 35.0 27 CAGTGAT 40 0.002409213 35.0 64 CAGCAAG 45 0.004290372 31.111113 1 CTTATAA 45 0.004290372 31.111113 1 GTATATC 45 0.004290372 31.111113 38 AACCTAG 45 0.004290372 31.111113 30 ATCCAAC 50 0.0071802824 27.999998 1 GAACGAA 50 0.0071802824 27.999998 26 TTCTACA 80 8.595387E-5 26.25 2 TATAAAC 70 0.0012189549 25.0 3 >>END_MODULE