FastQCFastQC Report
Wed 25 May 2016
SRR1780258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102626
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT1057910.308303938573072No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC28752.8014343343792025No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG26892.6201937130941477No Hit
CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG25412.4759807456200185RNA PCR Primer, Index 15 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC7060.6879348313292928No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT4090.39853448443864126RNA PCR Primer, Index 48 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1710.166624442149163No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT1600.15590591078284255No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1480.14421296747412934No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1310.12764796445345233No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGC1280.12472472862627404No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT1090.10621090172081148RNA PCR Primer, Index 48 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA150.00221911870.06
GCTTATA150.00221911870.03
TCTTTAT850.057.647061
CTTTATA900.054.4444472
CTAGAAT200.006934095652.513
CTGAGAT200.006934095652.562
ATCGTGT200.006934095652.531
CATTAGG200.006934095652.535
ATATCGT200.006934095652.539
GCATTAG200.006934095652.534
TTCGGTA200.006934095652.526
TGGGCTA200.006934095652.541
ATACGGG200.006934095652.51
CGGTAAG200.006934095652.528
AAACTCG352.0740827E-550.036
TTTATAC1200.049.5833323
TCCGGCA305.854265E-446.6666642
TTAATAC305.854265E-446.6666643
ACTCGTA404.5654677E-543.7538
CAGCTTA350.001250432140.01
Produced by FastQC (version 0.11.5)