FastQCFastQC Report
Wed 25 May 2016
SRR1780257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404310
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGT250.002344039535.22110738
GGCATAA250.002361185835.1688341
AATTATC353.2214785E-431.41628630
CTATACA502.374949E-630.768921
GTATAAA454.091004E-529.3037361
GCTACCA300.005768940329.3037363
CTTAGGC406.9791445E-427.53013214
TACACAG407.065551E-427.4722525
TTATCAA450.001402820924.4258232
CAAGGAA450.001404844624.4197794
TCAGTTT450.001404844624.4197799
ATAAAAC551.5846E-424.0143940
ATAAACT651.8237133E-523.6684023
CGAAACT500.002583351521.99139825
CAATTAT500.002583351521.99139829
TATGCAG500.002583351521.99139830
TAGATCT1351.10958354E-1021.1978939
AAACTCG550.004488681419.9921827
GGTAATA550.00450478119.9798181
TTACGAG550.00450478119.9798183
Produced by FastQC (version 0.11.5)