FastQCFastQC Report
Wed 25 May 2016
SRR1780257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404310
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT220845.462145383492864No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC66081.6343894536370605No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG58451.4456728747743068No Hit
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG45551.1266107689644085RNA PCR Primer, Index 13 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC12890.31881477084415427No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT6430.15903638297346095TruSeq Adapter, Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA357.27485E-637.717043
TCTTCTG4350.037.42339744
GTCTTCT4500.036.1759543
CTTTATA1550.035.486462
CTACGGA250.00235010435.202573
TCTATAG250.00235010435.202573
ATGCCGT30800.033.9982844
TCTTTAT1650.033.3935971
TTTATAC1850.032.1104553
CCGTCTT5250.031.00795641
CGTCTTC5450.029.87004742
CTTATAA300.005690091829.3863621
CAGCTTA454.013155E-529.3863621
ACGTGTA300.005738500529.3354782
CTTGTCA300.005741970629.33184810
ATTAACG300.005741970629.33184818
AATATCG300.005741970629.33184836
CGCTAAA300.005741970629.33184837
GTCGTTA300.005741970629.33184814
TTTATGA300.005741970629.33184829
Produced by FastQC (version 0.11.5)