FastQCFastQC Report
Wed 25 May 2016
SRR1780256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171054
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATGA252.3852682E-455.99999640
GGAGCGA200.006941976552.57
GTACACT350.001252775240.036
ATGGTTG350.001252775240.046
TTGCATT350.001252775240.013
CGTCCAA350.001252775240.017
TCCAGTA400.002413700635.032
TGTTACG400.002413700635.048
ACATGAG400.002413700635.027
CGGTCAG400.002413700635.027
CGACTTG400.002413700635.053
GCCGTAT501.7044156E-435.054
CGCCGTA552.982889E-431.81818453
CAAGTGC450.004298314531.11111321
ACTTACC450.004298314531.11111340
GTTACGA450.004298314531.11111349
TGACAGC450.004298314531.11111320
TAATACA802.4524306E-630.6254
CTTGTCT604.966787E-429.16666656
TTGTCTC604.966787E-429.16666657