FastQCFastQC Report
Wed 25 May 2016
SRR1780256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171054
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT81314.753469664550376No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC23211.3568814526406867No Hit
CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG22071.2902358319594982TruSeq Adapter, Index 2 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG21021.2288517076478773No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5050.29522840740351No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2760.16135255533340348TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT1800.1052299273913501No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTAA150.002221659570.021
TCGGCTA252.3852682E-455.99999626
CCTTTAT200.006941976552.562
CTTTATA1150.048.6956522
TGTATTC305.8653025E-446.66666421
TAGGGCG305.8653025E-446.66666440
TCTTTAT1200.046.6666641
TTTATAC1400.042.53
TAATACG350.001252775240.04
CGTATTC350.001252775240.060
GAATATG350.001252775240.01
CGGCTAT350.001252775240.027
TAGTGAA350.001252775240.09
TTCGGCT459.169089E-538.8888925
TAGACAT400.002413700635.05
ATAATGT400.002413700635.011
ATTCGGC400.002413700635.024
TTACTCG400.002413700635.019
TATTCGG400.002413700635.023
TCAGATG400.002413700635.04