##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780256_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 171054 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.72026377635133 34.0 34.0 34.0 34.0 34.0 2 33.75627579594748 34.0 34.0 34.0 34.0 34.0 3 33.782822968185485 34.0 34.0 34.0 34.0 34.0 4 33.782022051515895 34.0 34.0 34.0 34.0 34.0 5 33.78185836051773 34.0 34.0 34.0 34.0 34.0 6 37.55397710664469 38.0 38.0 38.0 37.0 38.0 7 37.53947291498591 38.0 38.0 38.0 37.0 38.0 8 37.46558981374303 38.0 38.0 38.0 37.0 38.0 9 37.4823096799841 38.0 38.0 38.0 37.0 38.0 10-11 37.44160323640488 38.0 38.0 38.0 37.0 38.0 12-13 37.42267061863505 38.0 38.0 38.0 37.0 38.0 14-15 37.34889859342664 38.0 38.0 38.0 37.0 38.0 16-17 37.39549498988623 38.0 38.0 38.0 37.0 38.0 18-19 37.36480000467689 38.0 38.0 38.0 36.5 38.0 20-21 37.37532007436248 38.0 38.0 38.0 37.0 38.0 22-23 37.35522408128428 38.0 38.0 38.0 37.0 38.0 24-25 37.3647561588738 38.0 38.0 38.0 36.5 38.0 26-27 37.27819285138027 38.0 38.0 38.0 36.5 38.0 28-29 37.25934207910952 38.0 38.0 38.0 36.0 38.0 30-31 37.216042887041525 38.0 38.0 38.0 36.0 38.0 32-33 37.20423667379892 38.0 38.0 38.0 36.0 38.0 34-35 37.22553988798859 38.0 38.0 38.0 36.0 38.0 36-37 37.17544751949677 38.0 38.0 38.0 36.0 38.0 38-39 36.83402609702199 38.0 38.0 38.0 35.0 38.0 40-41 37.03818969448244 38.0 38.0 38.0 36.0 38.0 42-43 37.15562044734411 38.0 38.0 38.0 36.0 38.0 44-45 37.20174330913045 38.0 38.0 38.0 36.0 38.0 46-47 37.15153401849708 38.0 38.0 38.0 36.0 38.0 48-49 37.16714312439346 38.0 38.0 38.0 36.0 38.0 50-51 37.05496802179429 38.0 38.0 38.0 36.0 38.0 52-53 37.05132005097805 38.0 38.0 38.0 36.0 38.0 54-55 37.01281466671344 38.0 38.0 38.0 36.0 38.0 56-57 37.060106749915235 38.0 38.0 38.0 36.0 38.0 58-59 36.9702316227624 38.0 38.0 38.0 36.0 38.0 60-61 36.987457177265654 38.0 38.0 38.0 36.0 38.0 62-63 36.96098600441966 38.0 38.0 38.0 36.0 38.0 64-65 36.917958656330754 38.0 38.0 38.0 35.5 38.0 66-67 36.659095958001565 38.0 37.5 38.0 34.5 38.0 68-69 36.5971944532136 38.0 38.0 38.0 34.0 38.0 70-71 36.099068130531876 38.0 37.0 38.0 32.5 38.0 72-73 35.295891940556785 38.0 37.0 38.0 26.5 38.0 74-75 35.04553825107861 38.0 37.0 38.0 25.0 38.0 76 34.3554374641926 38.0 35.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11112735987603628 1101 2 -0.03297272765262704 1101 3 0.08830662922479604 1101 4 0.008897813270131394 1101 5 -0.05349800430531815 1101 6 -0.25777419038022487 1101 7 -0.12450910804476933 1101 8 -0.07613247885164753 1101 9 -0.016601540658101044 1101 10-11 -0.0019718593794735284 1101 12-13 -0.026446665126947266 1101 14-15 0.10009407605751619 1101 16-17 0.1369505623506697 1101 18-19 0.08848291599950642 1101 20-21 0.07623368557374732 1101 22-23 -0.03254225985250514 1101 24-25 -0.06384447177692465 1101 26-27 -0.004062379394994764 1101 28-29 -0.07546690782861987 1101 30-31 -0.09196039309932758 1101 32-33 -0.0023259727189994805 1101 34-35 0.04006315203466215 1101 36-37 0.17279666536023086 1101 38-39 0.3681404083968687 1101 40-41 0.04499569588112706 1101 42-43 -0.009759661919950702 1101 44-45 0.01672820128177932 1101 46-47 0.03431061819601666 1101 48-49 0.19130105732008928 1101 50-51 0.1684246760339576 1101 52-53 0.169206876628202 1101 54-55 0.12385816138991146 1101 56-57 0.2624220247733291 1101 58-59 0.09607261646517884 1101 60-61 0.0495718280952957 1101 62-63 0.018729486921266414 1101 64-65 0.019521436419786653 1101 66-67 0.17511506480020955 1101 68-69 -0.1769163799477198 1101 70-71 -0.04237177555866367 1101 72-73 0.16901002280528132 1101 74-75 0.11649126557617251 1101 76 -0.6503515777831907 1102 1 -0.015340296161696187 1102 2 0.02705588938019332 1102 3 -0.1560694006526262 1102 4 0.06338898279764038 1102 5 0.026846639815538254 1102 6 0.022342137341617274 1102 7 0.1517692313408432 1102 8 0.04491653685672503 1102 9 0.11687573876088209 1102 10-11 0.05048925709805019 1102 12-13 0.10559459712181507 1102 14-15 0.05681985359883157 1102 16-17 0.17710222229353434 1102 18-19 0.15795153799123796 1102 20-21 0.148192092731108 1102 22-23 0.18011741957474214 1102 24-25 0.10048393191952698 1102 26-27 0.19112747963770715 1102 28-29 0.17954494706133062 1102 30-31 0.23618502688403709 1102 32-33 0.2548192458087257 1102 34-35 0.23723140882346172 1102 36-37 0.21312395330233613 1102 38-39 0.11287027703745878 1102 40-41 0.020348372400178505 1102 42-43 0.2637912779481155 1102 44-45 0.27248130963909034 1102 46-47 0.23055424522414114 1102 48-49 0.2266642715190983 1102 50-51 0.29972985034473965 1102 52-53 0.14582252508292726 1102 54-55 0.3894774308807456 1102 56-57 0.0390438718633348 1102 58-59 0.2909912852049388 1102 60-61 0.08332339712485037 1102 62-63 0.08791917032597496 1102 64-65 0.27693784524044673 1102 66-67 0.27574216006807006 1102 68-69 0.21478054811196046 1102 70-71 0.35841390146143937 1102 72-73 0.05586684982719703 1102 74-75 -0.10352671046610595 1102 76 0.3469055261111009 1103 1 0.007364989523274801 1103 2 0.06580682993700293 1103 3 0.03438724578595753 1103 4 0.025668990224374966 1103 5 0.008485016487774999 1103 6 -0.0498779676098664 1103 7 0.02728836983966687 1103 8 -0.0412775549807094 1103 9 -0.08360699896790891 1103 10-11 -0.07339795426094753 1103 12-13 -0.00900186736178199 1103 14-15 0.14419147370183083 1103 16-17 8.321041498291493E-4 1103 18-19 0.10719322933225328 1103 20-21 0.1056129638088592 1103 22-23 0.12878744781588125 1103 24-25 -0.012099496690993305 1103 26-27 0.03949017788754361 1103 28-29 0.1565880318922268 1103 30-31 0.171824068053553 1103 32-33 0.14698257533096637 1103 34-35 -0.033299602678212636 1103 36-37 0.10375796188216668 1103 38-39 0.1116048006026773 1103 40-41 0.07909774943398418 1103 42-43 -0.0019758743878881546 1103 44-45 -0.028738726908258627 1103 46-47 0.10480057827408018 1103 48-49 0.13886512559354003 1103 50-51 -0.010253244062731426 1103 52-53 -0.2662122046718949 1103 54-55 -0.2645857745368616 1103 56-57 -0.17946579754926972 1103 58-59 0.10228627313649952 1103 60-61 -0.0569221737076262 1103 62-63 -0.1996053818712653 1103 64-65 -0.09794366903158647 1103 66-67 -0.3194934921653214 1103 68-69 -0.2598732781220079 1103 70-71 -0.31250115037911286 1103 72-73 -0.2507448980937994 1103 74-75 -0.4030610737702247 1103 76 -0.056331943717786714 1104 1 0.06429643017564501 1104 2 -0.07830874441113167 1104 3 -0.14753161055150343 1104 4 -0.0041306674822649825 1104 5 -0.026952414298492045 1104 6 0.09433828125754928 1104 7 -0.009608841476151042 1104 8 -0.14008959565321533 1104 9 -0.0022619873903408916 1104 10-11 -0.05706351350789873 1104 12-13 0.06227569228786933 1104 14-15 0.04613449192391528 1104 16-17 -0.04862425344606436 1104 18-19 0.04862582774158852 1104 20-21 0.08842278964194605 1104 22-23 0.09060663637924904 1104 24-25 0.073121483878829 1104 26-27 0.06531319208264108 1104 28-29 0.026692733392273738 1104 30-31 -0.07777323386932977 1104 32-33 -0.0869095015843655 1104 34-35 -0.10037400114795503 1104 36-37 -0.0667595051469263 1104 38-39 -0.10862071032050835 1104 40-41 -0.10936495065662655 1104 42-43 -0.12265945437378889 1104 44-45 -0.15495940545451958 1104 46-47 -0.36185098721469444 1104 48-49 -0.2230849398448953 1104 50-51 -0.3600971985578454 1104 52-53 -0.18313055763675834 1104 54-55 -0.37399380836350105 1104 56-57 -0.357533853524302 1104 58-59 -0.04537807801622762 1104 60-61 -0.1296846825166469 1104 62-63 -0.0649783809651936 1104 64-65 -0.46840073134911364 1104 66-67 -0.722418086950384 1104 68-69 -0.4070845939379737 1104 70-71 -0.40645564549640056 1104 72-73 -0.2796888223865608 1104 74-75 -0.49621017273327084 1104 76 -0.2831012983941221 1105 1 -0.2658749284534849 1105 2 -0.06796509988755872 1105 3 -0.024965681988021515 1105 4 -0.05774978398387276 1105 5 0.045281553284311826 1105 6 -0.14602743218038228 1105 7 -0.17113382200357563 1105 8 -0.09482050326050029 1105 9 -0.03433609443384711 1105 10-11 0.09399495984249029 1105 12-13 -0.02849662698811528 1105 14-15 -0.10485443652829218 1105 16-17 0.01595513900970502 1105 18-19 -0.0729873357167321 1105 20-21 -0.12947760351854498 1105 22-23 -0.018669262140832643 1105 24-25 0.1337623169339821 1105 26-27 0.14320115988799387 1105 28-29 0.13448732558099863 1105 30-31 -0.093056884327396 1105 32-33 -0.036174180194208816 1105 34-35 -0.009803362076709732 1105 36-37 -0.11067015386174006 1105 38-39 0.04152904302691951 1105 40-41 -0.1487018632340522 1105 42-43 0.05364613518149497 1105 44-45 -0.049728701845602075 1105 46-47 -0.10685070903510052 1105 48-49 -0.04968864519707239 1105 50-51 0.01224618633143848 1105 52-53 0.19375874543063532 1105 54-55 -0.16919103769475186 1105 56-57 0.1896245419358067 1105 58-59 0.1465588864880587 1105 60-61 0.17643109582984806 1105 62-63 0.05157655023172225 1105 64-65 -0.24430388776023193 1105 66-67 -0.32946159423868693 1105 68-69 0.02517172073878271 1105 70-71 -0.13633630645110628 1105 72-73 0.41943908459018076 1105 74-75 0.3159096484899777 1105 76 0.5177452948331336 1106 1 0.00794000845682774 1106 2 0.06616680113930329 1106 3 0.026131282886368012 1106 4 0.015243850209600396 1106 5 2.8515299633369295E-4 1106 6 0.048338071210928035 1106 7 0.058418866412928594 1106 8 0.08738176266349029 1106 9 0.040774738804756794 1106 10-11 0.01863546228651103 1106 12-13 0.015976394117558357 1106 14-15 0.0707807231852442 1106 16-17 0.08636266430242046 1106 18-19 0.05217607226325782 1106 20-21 0.1251238704687978 1106 22-23 -0.005035352853575148 1106 24-25 -0.017143768473928844 1106 26-27 -0.1760577730815811 1106 28-29 0.10604012767262105 1106 30-31 -0.0795129678407136 1106 32-33 -0.07835238488519991 1106 34-35 -0.03157688335289777 1106 36-37 -0.0901660047199826 1106 38-39 -0.21544539901771742 1106 40-41 0.05295542523550267 1106 42-43 0.1424216647180785 1106 44-45 0.11563543744430405 1106 46-47 -0.09335889364349015 1106 48-49 0.048505341389265766 1106 50-51 -0.10105948605687587 1106 52-53 -0.07029670908037389 1106 54-55 0.15172092092750233 1106 56-57 -0.009669251402833368 1106 58-59 -0.068407863540358 1106 60-61 -0.04375050016879101 1106 62-63 -0.12024575661531145 1106 64-65 0.11200602849910268 1106 66-67 0.08693035756306244 1106 68-69 0.1627399258086939 1106 70-71 0.03691167581556698 1106 72-73 -0.4322077836603597 1106 74-75 -0.6992636113334427 1106 76 -0.6574043774036156 1107 1 -0.26234895666125624 1107 2 -0.07907334435493851 1107 3 -0.0844198730211616 1107 4 -0.13014687147384052 1107 5 0.0027978931535912466 1107 6 -0.04208293040306188 1107 7 0.05073006975195682 1107 8 0.072648823651555 1107 9 0.010584212870362819 1107 10-11 -0.1710764195864698 1107 12-13 -0.13905590918052724 1107 14-15 -0.09988797625997847 1107 16-17 -0.13599131208535198 1107 18-19 -0.2121557223696442 1107 20-21 0.07053529637826017 1107 22-23 -0.007639850376122581 1107 24-25 0.10665103382424945 1107 26-27 0.14166033888123053 1107 28-29 0.015435611328044274 1107 30-31 -0.1427573200572425 1107 32-33 0.02604632204177193 1107 34-35 -0.25855637205262383 1107 36-37 -0.009835960535291122 1107 38-39 -0.0686289090427934 1107 40-41 0.06452626427426367 1107 42-43 -0.11868215285887374 1107 44-45 -0.09199736369630784 1107 46-47 0.002934579349258115 1107 48-49 0.032132533566830546 1107 50-51 0.09896215927032159 1107 52-53 -0.03881922727891407 1107 54-55 0.16733143679211793 1107 56-57 -0.1007019687854438 1107 58-59 0.09922343183836091 1107 60-61 -0.31573580016831926 1107 62-63 -0.09008581646934744 1107 64-65 0.23621726807052568 1107 66-67 -0.036318606049263735 1107 68-69 0.1573168302445751 1107 70-71 -0.306693776924682 1107 72-73 -0.29420470625949235 1107 74-75 0.004183642298023926 1107 76 -0.06092974243323823 1108 1 -0.08415986493261585 1108 2 0.019111427638151213 1108 3 0.1192040094820328 1108 4 0.030725715108680163 1108 5 0.017982702709595344 1108 6 -0.07154164875630187 1108 7 0.16700138670785947 1108 8 0.07971065221439488 1108 9 -0.037965858499184435 1108 10-11 -0.08369333416839453 1108 12-13 -0.10678231966809903 1108 14-15 0.11945363969675071 1108 16-17 0.04760214445435196 1108 18-19 0.1504132994919729 1108 20-21 -0.005233804365495587 1108 22-23 -0.0035830552442774888 1108 24-25 0.19011818505255462 1108 26-27 0.2332457757379629 1108 28-29 0.2001066540746379 1108 30-31 0.08402644900593259 1108 32-33 0.07228392627887104 1108 34-35 0.01970737597532235 1108 36-37 0.04002176991321704 1108 38-39 -0.10358589950181596 1108 40-41 -0.02587594066901744 1108 42-43 0.05559335236237217 1108 44-45 -0.024017873049473337 1108 46-47 -0.1290262988717643 1108 48-49 -0.06732702995206097 1108 50-51 0.0660687816127421 1108 52-53 -0.006691194073290774 1108 54-55 -0.267818533157552 1108 56-57 0.061335068251594294 1108 58-59 0.008400991751216225 1108 60-61 0.1008299161565347 1108 62-63 0.13226318060075215 1108 64-65 0.33074904629906143 1108 66-67 0.10151617345641739 1108 68-69 0.0641349693776192 1108 70-71 0.58093910189735 1108 72-73 0.39465460183156154 1108 74-75 0.2752071379031662 1108 76 0.9704071218143824 1109 1 0.05162073377901777 1109 2 0.148957013087184 1109 3 0.15473356613227907 1109 4 0.04058740514278725 1109 5 0.15260756061031344 1109 6 -0.08793990567180288 1109 7 0.15938960194090157 1109 8 0.08842607472292485 1109 9 0.1688738535129417 1109 10-11 -0.07401400487700016 1109 12-13 0.07461451625459858 1109 14-15 0.02204695155730718 1109 16-17 0.13884409216181126 1109 18-19 0.09908467122369302 1109 20-21 0.17762428582017975 1109 22-23 0.06992963895807236 1109 24-25 0.14580926121776372 1109 26-27 0.013316351713712038 1109 28-29 -0.04709162178742332 1109 30-31 0.10607315230264192 1109 32-33 0.02788500623339729 1109 34-35 -0.04557066494926687 1109 36-37 0.07410428222848253 1109 38-39 0.1598898488877296 1109 40-41 0.18399285432909096 1109 42-43 0.0397990173870042 1109 44-45 0.1252905771210422 1109 46-47 0.2767453002188063 1109 48-49 0.05173558846400539 1109 50-51 0.1398633060538188 1109 52-53 0.17672818826850545 1109 54-55 0.005194445243354551 1109 56-57 0.18029111220764094 1109 58-59 0.13353835438857686 1109 60-61 0.3547494879640354 1109 62-63 0.08456045229456777 1109 64-65 0.0351399140519959 1109 66-67 -0.11558073825257509 1109 68-69 -0.19875106699979028 1109 70-71 -0.08271427059223413 1109 72-73 -0.5658631132120178 1109 74-75 -0.2547260754352223 1109 76 0.26910928172343773 1110 1 -0.021904456234395298 1110 2 -0.060888387764642005 1110 3 0.06198348965629208 1110 4 -0.030292892289971007 1110 5 0.047065992970246384 1110 6 0.010871775152729413 1110 7 0.010301431011185969 1110 8 0.09757966203496693 1110 9 0.16466649769959218 1110 10-11 -0.03473123197537831 1110 12-13 0.10839572433987144 1110 14-15 -0.07089607971548872 1110 16-17 8.390565122411431E-4 1110 18-19 -0.01136077198406582 1110 20-21 0.12845610924301099 1110 22-23 -0.0988352150316274 1110 24-25 0.01107504468483711 1110 26-27 -0.0686329623555153 1110 28-29 0.11265141889779784 1110 30-31 0.04688779804233434 1110 32-33 0.09205659481612116 1110 34-35 -0.015123809877145789 1110 36-37 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48-49 -0.018081093709653828 2115 50-51 -0.07528241778755529 2115 52-53 0.028474611181970033 2115 54-55 0.10211903095513009 2115 56-57 0.1903396449106367 2115 58-59 -0.2860784132831071 2115 60-61 -0.11061123826626584 2115 62-63 0.08852353726451412 2115 64-65 0.12478459431452649 2115 66-67 0.07557273070410275 2115 68-69 0.07479986719187082 2115 70-71 -0.38562120347628337 2115 72-73 -0.10215702524963888 2115 74-75 0.04784137999264004 2115 76 0.17573537873855116 2116 1 0.004192363062976767 2116 2 0.024710939526045195 2116 3 -0.03241581416778416 2116 4 0.09608943735715059 2116 5 0.08181123364960285 2116 6 0.09081467308277524 2116 7 0.07541147960661476 2116 8 0.22205044122399897 2116 9 0.07591384548444324 2116 10-11 0.1396720807268963 2116 12-13 0.14497429346369017 2116 14-15 0.24519663292616656 2116 16-17 -0.07545918447022615 2116 18-19 0.17877005433373938 2116 20-21 -0.06289204716190255 2116 22-23 0.11289466479981058 2116 24-25 0.17066750799382646 2116 26-27 0.17800349105839075 2116 28-29 0.2871549535550386 2116 30-31 0.14638375494200773 2116 32-33 0.2872156299475819 2116 34-35 0.23349752713261296 2116 36-37 0.09321713284818856 2116 38-39 0.23873223937395238 2116 40-41 0.014014430926010846 2116 42-43 0.20682445770011526 2116 44-45 0.09732128613805457 2116 46-47 0.19828928135182622 2116 48-49 0.1470338974609433 2116 50-51 0.06860283136868617 2116 52-53 0.02257093000165611 2116 54-55 0.1749119423581149 2116 56-57 -0.17211242033287988 2116 58-59 0.13711103741331954 2116 60-61 0.07809235514251611 2116 62-63 0.33385544953477364 2116 64-65 0.15328683562357526 2116 66-67 0.4392238179218495 2116 68-69 0.2353826075448353 2116 70-71 0.4930346380333859 2116 72-73 0.7456377147271667 2116 74-75 0.6743421166466632 2116 76 0.8137979264120787 2117 1 -0.003790521632232924 2117 2 0.05997932410949147 2117 3 0.02772723912595154 2117 4 0.13123009578547595 2117 5 0.05110905911181618 2117 6 0.0838216660897686 2117 7 0.08173392428522419 2117 8 -0.4277244414275927 2117 9 -0.10092301628392875 2117 10-11 0.07536994450695289 2117 12-13 -0.03147938983930487 2117 14-15 0.14802097753132415 2117 16-17 0.060688914006640005 2117 18-19 -0.040575668436645174 2117 20-21 -0.09626848807258881 2117 22-23 0.09773516676359861 2117 24-25 -0.024753509345003977 2117 26-27 0.013516151913513852 2117 28-29 -0.5780939527897573 2117 30-31 -0.1610930148635319 2117 32-33 -0.05839538004698852 2117 34-35 0.07550825612965184 2117 36-37 0.11999172811593439 2117 38-39 0.05449524349312185 2117 40-41 -0.25556758523134704 2117 42-43 -0.050344172756183525 2117 44-45 -0.04830249647202578 2117 46-47 -0.06028433681083101 2117 48-49 0.06568830241671719 2117 50-51 0.27396254015305743 2117 52-53 0.03357134511166038 2117 54-55 0.06583380588271837 2117 56-57 -0.001893369976841086 2117 58-59 0.19794061879083813 2117 60-61 -0.1775515443369997 2117 62-63 -0.019035951301837883 2117 64-65 -0.09286541395332648 2117 66-67 0.20673028405845173 2117 68-69 0.10700983876110826 2117 70-71 0.11482152694356529 2117 72-73 -0.5419536893025878 2117 74-75 -0.31872873943787994 2117 76 -1.0591624465482923 2118 1 -0.040218574453732 2118 2 -0.2780651450825147 2118 3 -0.19327580154921975 2118 4 -0.10796240570210358 2118 5 -0.14870751775394098 2118 6 -0.06765527227241108 2118 7 0.005111093564032387 2118 8 -0.11838350913583895 2118 9 -0.12015515027015766 2118 10-11 -0.03686754477971732 2118 12-13 -0.041256497977073536 2118 14-15 0.015669722229255 2118 16-17 0.05581400749238696 2118 18-19 -0.11750693389249989 2118 20-21 -0.14772249854299702 2118 22-23 -0.08292570242186059 2118 24-25 -0.04548960385159262 2118 26-27 -0.12923454001750656 2118 28-29 0.010519619430375826 2118 30-31 -0.13164978050226495 2118 32-33 -0.21120704925210276 2118 34-35 -0.08462669089054486 2118 36-37 -0.04720719318134314 2118 38-39 -0.0655650241081247 2118 40-41 -0.05407235914596242 2118 42-43 -0.1271799053296192 2118 44-45 0.04132372430173348 2118 46-47 -0.10556419356609581 2118 48-49 -0.23752760735656864 2118 50-51 -0.13668146065487718 2118 52-53 0.02697138769202212 2118 54-55 0.1493486516926481 2118 56-57 -0.03556188725191589 2118 58-59 -0.16563296609422196 2118 60-61 0.033365953465740006 2118 62-63 -0.10598179234603577 2118 64-65 0.018602353252141768 2118 66-67 0.0467864028030931 2118 68-69 -0.236329881627789 2118 70-71 0.07687859555800713 2118 72-73 -0.02098831587819916 2118 74-75 -0.3083020076996803 2118 76 -0.7252340798330366 2119 1 0.1369244960759488 2119 2 0.10491085423464597 2119 3 0.09114749389016907 2119 4 0.13276993787201974 2119 5 -0.0431770647870664 2119 6 0.06651702691724637 2119 7 0.007906460936119686 2119 8 0.12479744822413608 2119 9 0.19078679251016695 2119 10-11 0.03807390721091508 2119 12-13 0.014930919668955767 2119 14-15 -0.30740059406335973 2119 16-17 0.05820127256682639 2119 18-19 0.19575292324502414 2119 20-21 0.16194179063882785 2119 22-23 0.07999246906645396 2119 24-25 -0.009328539478119069 2119 26-27 0.25331735299034364 2119 28-29 0.3759152119255589 2119 30-31 0.08865344217221605 2119 32-33 -0.14096277468727436 2119 34-35 0.2552078276287659 2119 36-37 -0.007639941656968574 2119 38-39 0.23633731941264102 2119 40-41 0.11757066603901478 2119 42-43 -0.11630251206304365 2119 44-45 0.04087294463963076 2119 46-47 0.12461883898072301 2119 48-49 0.06196613240753379 2119 50-51 0.21752732768139538 2119 52-53 0.16134106969231965 2119 54-55 0.18814771065675728 2119 56-57 0.038840763012416346 2119 58-59 0.10279165234346976 2119 60-61 0.37983146748792507 2119 62-63 0.16121318452817235 2119 64-65 -0.16799908817583997 2119 66-67 -0.10083193352654263 2119 68-69 0.007990027896198626 2119 70-71 0.09775582878447153 2119 72-73 0.23763384541022958 2119 74-75 0.39593852192641776 2119 76 -0.5647138427602769 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 1.0 22 10.0 23 17.0 24 37.0 25 65.0 26 123.0 27 213.0 28 441.0 29 679.0 30 1106.0 31 1781.0 32 2835.0 33 4772.0 34 7909.0 35 13157.0 36 21815.0 37 116091.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.69043693804296 18.024717340722813 12.33703976522034 31.94780595601389 2 19.772118746126953 19.440059864136472 35.59168449729325 25.19613689244332 3 18.636804751715832 20.321652811392894 31.497071100354272 29.544471336536997 4 14.667298046231014 21.97610111426801 33.96880517263554 29.387795666865436 5 15.119786734013822 31.464917511429142 37.68108316671928 15.73421258783776 6 28.65410922866463 37.39988541630129 17.516690635705682 16.4293147193284 7 27.120090731581843 26.53781846668303 22.448466566113627 23.8936242356215 8 23.457504647655124 36.11315724858816 17.694412290855517 22.73492581290119 9 22.965262431746698 16.446268429852562 19.14716989956388 41.44129923883686 10-11 25.916669589720204 26.202836531153906 24.575280320834356 23.305213558291534 12-13 25.215429045798405 20.460790159832566 25.793316730389233 28.530464063979792 14-15 22.371005647339437 20.470143931156244 21.5727197259345 35.58613069556982 16-17 26.284097419528337 25.668210038935076 21.31841406807207 26.729278473464518 18-19 27.694470751926293 24.679048721456383 22.005331649654494 25.621148876962828 20-21 24.09589954049598 23.297321313737182 21.86444046909163 30.742338676675203 22-23 23.876962830451205 25.457165573444644 21.945701357466064 28.720170238638094 24-25 26.97159961181849 26.417973271598445 21.698118722742528 24.91230839384054 26-27 26.581956575116628 27.23847439989711 22.335929004875656 23.84364002011061 28-29 26.895892525167493 23.870532112666176 22.90446291814281 26.329112444023522 30-31 26.98592257415787 25.957300033907423 22.962047072854187 24.094730319080522 32-33 23.69573351105499 26.130052498041557 23.116091994340966 27.05812199656249 34-35 25.157260280379294 23.82785553100191 23.698948869947504 27.315935318671297 36-37 25.18356776222713 24.180083482409064 26.579325826931843 24.057022928431955 38-39 21.897763279432226 24.642802857577138 28.1627439288178 25.296689934172832 40-41 22.294129339272978 21.488828089375282 28.26242005448572 27.954622516866017 42-43 25.193213838904672 21.512504823038338 27.609994504659348 25.68428683339764 44-45 21.468074409250878 25.04209197095654 29.50354858699592 23.986285032796662 46-47 23.910870251499528 22.828755831491808 27.938253417049587 25.322120499959077 48-49 23.509827305996936 21.894840225893578 25.70504051352205 28.89029195458744 50-51 21.77411811474739 22.295883171396166 28.083821483274285 27.846177230582153 52-53 19.338045295637635 22.337682836998844 33.22138038280309 25.10289148456043 54-55 19.65636582599647 23.16753773662118 30.032913582845183 27.143182854537162 56-57 22.001239374700386 23.895962678452417 26.217744104201014 27.88505384264618 58-59 20.419575104937625 23.800086522384746 29.892899318343915 25.887439054333722 60-61 22.627649748032784 26.213359523893036 26.32823552796193 24.830755200112247 62-63 19.67156570439744 31.5143171162323 23.386474446665964 25.427642732704292 64-65 19.144539151379096 32.974966969495014 22.521835209933705 25.358658669192184 66-67 19.40498322167269 32.58678545956248 22.308159996258492 25.70007132250634 68-69 19.492674827832147 32.957720953616985 22.19269938148187 25.356904837068996 70-71 20.019701380850492 32.161481169689104 21.969085785775253 25.849731663685148 72-73 20.247699556865083 30.429864253393664 22.19562243502052 27.12681375472073 74-75 20.307329849053517 29.72774679341027 22.315759935458978 27.649163422077237 76 20.516912787774622 29.425210752160137 22.54668116501222 27.511195295053025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.5 12 3.0 13 5.0 14 5.0 15 9.0 16 19.5 17 24.0 18 21.0 19 26.5 20 47.0 21 59.0 22 81.0 23 134.5 24 195.5 25 225.0 26 322.5 27 512.0 28 669.5 29 735.0 30 923.0 31 1186.5 32 1561.0 33 1860.0 34 2201.0 35 2903.5 36 3516.5 37 3768.0 38 4278.5 39 5377.5 40 6607.0 41 9376.0 42 11504.0 43 12466.5 44 13171.5 45 12002.0 46 11090.0 47 10591.5 48 9358.5 49 8066.5 50 7509.0 51 6940.5 52 5701.5 53 4671.0 54 4311.0 55 4100.5 56 3537.0 57 2995.5 58 2807.0 59 2660.5 60 2647.0 61 2946.0 62 3112.0 63 3220.5 64 3322.0 65 3366.5 66 3406.5 67 3395.0 68 3314.5 69 3047.5 70 2673.0 71 2485.0 72 2280.5 73 1809.5 74 1267.5 75 992.0 76 811.0 77 442.0 78 176.0 79 98.0 80 61.0 81 14.5 82 3.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 171054.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.07771814748559 #Duplication Level Percentage of deduplicated Percentage of total 1 57.964813595835075 22.65132648169584 2 20.87846328765484 16.31765407415202 3 8.077015139728323 9.46893963309832 4 3.7520196277900784 5.864814619944579 5 1.9792352342768236 3.8671998316321163 6 1.4331877206630363 3.3603423480304464 7 1.0816228831308718 2.958714791820127 8 0.8646998982706002 2.703239912542238 9 0.6747052839447071 2.3729348626749447 >10 3.253844772904075 20.414021303214188 >50 0.023936329363892048 0.5799338220678849 >100 0.008976123511459518 0.5162112549253451 >500 0.001496020585243253 0.29522840740351 >1k 0.004488061755729759 3.875968992248062 >5k 0.001496020585243253 4.753469664550376 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 8131 4.753469664550376 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 2321 1.3568814526406867 No Hit CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG 2207 1.2902358319594982 TruSeq Adapter, Index 2 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 2102 1.2288517076478773 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 505 0.29522840740351 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 276 0.16135255533340348 TruSeq Adapter, Index 2 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT 180 0.1052299273913501 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013446046277783624 0.0 2 0.0 0.0 0.0 0.019292153355080853 0.0 3 0.0 0.0 0.0 0.02104598547827002 0.0 4 0.0 0.0 0.0 0.09879920960632314 0.0 5 0.0 0.0 0.0 0.10698375951453927 0.0 6 0.0 0.0 0.0 0.10756837022226899 0.0 7 0.0 0.0 0.0 0.1104914237609176 0.0 8 0.0 0.0 0.0 0.1104914237609176 0.0 9 0.0 0.0 0.0 0.11692214154594456 0.0 10 0.0 0.0 0.0 0.12042980579232289 0.0 11 0.0 0.0 0.0 0.12159902720778233 0.0 12 0.0 0.0 0.0 0.12276824862324179 0.0 13 0.0 0.0 0.0 0.12335285933097151 0.0 14 0.0 0.0 0.0 0.12335285933097151 0.0 15 0.0 0.0 0.0 0.12452208074643095 0.0 16 0.0 0.0 0.0 0.12510669145416067 0.0 17 0.0 0.0 0.0 0.12686052357734984 0.0 18 0.0 0.0 0.0 0.12919896640826872 0.0 19 0.0 0.0 0.0 0.13679890560875513 0.0 20 0.0 0.0 0.0 0.13972195914740373 0.0 21 0.0 0.0 0.0 0.14206040197832265 0.0 22 0.0 0.0 0.0 0.14498345551697125 0.0 23 0.0 0.0 0.0 0.1490757304710793 0.0 24 0.0 0.0 0.0 0.15258339471745763 0.0 25 0.0 0.0 0.0 0.1590141125024846 0.0 26 0.0 0.0 0.0 0.17070632665707905 0.0 27 0.0 0.0 0.0 0.23735194733826745 0.0 28 0.0 0.0 0.0 0.31627439288178 0.0 29 0.0 0.0 0.0 0.4209197095654004 0.0 30 0.0 0.0 0.0 0.5138728120944264 0.0 31 0.0 0.0 0.0 0.6360564500099384 0.0 32 0.0 0.0 0.0 0.7687630806645854 0.0 33 0.0 0.0 0.0 0.9347925216598267 0.0 34 0.0 0.0 0.0 1.1867597366913372 0.0 35 0.0 0.0 0.0 1.541618436283279 0.0 36 0.0 0.0 0.0 2.037368316438084 0.0 37 0.0 0.0 0.0 2.732470447928724 0.0 38 0.0 0.0 0.0 3.456803114805851 0.0 39 0.0 0.0 0.0 4.2185508669776794 0.0 40 0.0 0.0 0.0 4.968021794287184 0.0 41 0.0 0.0 0.0 5.722754217966256 0.0 42 0.0 0.0 0.0 6.486255802261274 0.0 43 0.0 0.0 0.0 7.325756778561156 0.0 44 0.0 0.0 0.0 8.205011282986659 0.0 45 0.0 0.0 0.0 9.051527587779297 0.0 46 0.0 0.0 0.0 9.93312053503572 0.0 47 0.0 0.0 0.0 10.77729839699744 0.0 48 0.0 0.0 0.0 11.57529201304851 0.0 49 0.0 0.0 0.0 12.321255276111637 0.0 50 0.0 0.0 0.0 13.110479731546762 0.0 51 0.0 0.0 0.0 13.8763197586727 0.0 52 0.0 0.0 0.0 14.608252364750312 0.0 53 0.0 0.0 0.0 15.30744677119506 0.0 54 0.0 0.0 0.0 15.977410642253323 0.0 55 0.0 0.0 0.0 16.647374513311586 0.0 56 0.0 0.0 0.0 17.327861377108984 0.0 57 0.0 0.0 0.0 18.038163387000598 0.0 58 0.0 0.0 0.0 18.725080968583022 0.0 59 0.0 0.0 0.0 19.40089094671858 0.0 60 0.0 0.0 0.0 20.0562395500836 0.0 61 0.0 0.0 0.0 20.685865282308512 0.0 62 0.0 0.0 0.0 21.30029113613245 0.0 63 0.0 0.0 0.0 21.92991686835736 0.0 64 0.0 0.0 0.0 22.535573561565354 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTTAA 15 0.0022216595 70.0 21 TCGGCTA 25 2.3852682E-4 55.999996 26 CCTTTAT 20 0.0069419765 52.5 62 CTTTATA 115 0.0 48.695652 2 TGTATTC 30 5.8653025E-4 46.666664 21 TAGGGCG 30 5.8653025E-4 46.666664 40 TCTTTAT 120 0.0 46.666664 1 TTTATAC 140 0.0 42.5 3 TAATACG 35 0.0012527752 40.0 4 CGTATTC 35 0.0012527752 40.0 60 GAATATG 35 0.0012527752 40.0 1 CGGCTAT 35 0.0012527752 40.0 27 TAGTGAA 35 0.0012527752 40.0 9 TTCGGCT 45 9.169089E-5 38.88889 25 TAGACAT 40 0.0024137006 35.0 5 ATAATGT 40 0.0024137006 35.0 11 ATTCGGC 40 0.0024137006 35.0 24 TTACTCG 40 0.0024137006 35.0 19 TATTCGG 40 0.0024137006 35.0 23 TCAGATG 40 0.0024137006 35.0 4 >>END_MODULE