FastQCFastQC Report
Wed 25 May 2016
SRR1780255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608598
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT407.024392E-427.5024711
GTTATAC551.6083325E-423.9646841
TAGATTT651.8021528E-523.7080939
GTATAGC602.846477E-422.02735313
AACCAAG500.002569020322.01465636
TAGATCT1105.2004907E-922.01465639
ACCTTAA500.00259740221.97304332
TACGGTG602.8976233E-421.96943343
ATACAAT654.8519616E-420.3547421
TCCACAA654.9472094E-420.29615226
TTACGCC654.974712E-420.27947644
CAACGGT550.00444897920.02486613
AACAAGA708.19322E-418.83094244
TACTCGT600.00737956118.34554739
AGTTAGA600.00737956118.34554736
CAATGTG600.007460543418.31087132
TTTACGC600.007467619618.30786143
TAGTGAA600.007471159618.3063569
GTATGTA851.4455638E-418.0909881
TAGTGAT851.4455638E-418.0909884
Produced by FastQC (version 0.11.5)