FastQCFastQC Report
Wed 25 May 2016
SRR1780255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608598
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT153782.526791083769582No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC48050.7895195186313462No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG40650.6679285833998797No Hit
CACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTTCTG30230.49671540162800404RNA PCR Primer, Index 31 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC8590.14114407211328334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACAG254.4415865E-543.99794837
TCTATCG250.002352416335.19835729
ATGCCGT18850.032.79422844
TCTTCTG3700.032.70117644
CGTCTTC3800.032.4195442
TCTTTAT1700.031.1136131
CTTTATA1700.031.0599272
TATACCG502.3597677E-630.7985636
CCGTCTT4050.029.87514941
GGTCATA300.00569354129.3850781
GCCGTCT4300.028.13822240
TTGGTAA407.0300116E-427.49871433
GTAAACA407.0300116E-427.49871436
GTCTTCT4450.027.1897443
TATGCCG22950.026.83970543
TCGTCTT609.746125E-625.66546814
CAATCGA450.001397797324.44330438
AATCGAG450.001397797324.44330439
TGCCGTC5400.023.2211439
GGAAGAC500.002578447622.0007823
Produced by FastQC (version 0.11.5)