FastQCFastQC Report
Wed 25 May 2016
SRR1780254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73375
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA150.00221658770.018
AAAGATA150.00221658770.025
GGTGGTA200.006926247452.50000447
TAACAGA200.006926247452.50000444
ACGGATG200.006926247452.5000048
ACCAGTG200.006926247452.50000425
GTACTGG200.006926247452.50000419
TGAGATT200.006926247452.50000453
CGAACAT305.8432814E-446.66666425
TCTCGGT604.833055E-946.66666443
GACGACC503.4363748E-642.00000423
TATACCC350.001248099640.0000043
AGAGAAA350.001248099640.00000437
CGACGAA556.613898E-638.1818222
GTGGGAG400.002404746835.00000440
ATATCCT400.002404746835.00000449
ACGCTTC400.002404746835.00000415
ATCTCGG601.2011204E-535.042
TCGGTGG751.4667748E-632.66666845
AGATCTC652.0774542E-532.30769340
Produced by FastQC (version 0.11.5)