##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780254_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73375 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.432926746166952 34.0 27.0 34.0 12.0 34.0 2 29.563066439523 34.0 31.0 34.0 12.0 34.0 3 29.30085178875639 34.0 27.0 34.0 11.0 34.0 4 29.219638841567292 34.0 27.0 34.0 11.0 34.0 5 29.01643611584327 34.0 27.0 34.0 11.0 34.0 6 31.968095400340715 37.0 31.0 38.0 11.0 38.0 7 32.155584327086885 37.0 31.0 38.0 12.0 38.0 8 32.11517546848382 37.0 31.0 38.0 11.0 38.0 9 32.493069846678026 37.0 31.0 38.0 12.0 38.0 10-11 32.188129471890974 37.0 31.0 38.0 11.0 38.0 12-13 32.25458262350937 37.0 31.0 38.0 11.0 38.0 14-15 32.13002385008518 37.0 31.0 38.0 11.0 38.0 16-17 32.18455877342419 38.0 31.0 38.0 11.0 38.0 18-19 32.25525724020443 38.0 31.0 38.0 11.0 38.0 20-21 32.221478705281086 38.0 31.0 38.0 11.0 38.0 22-23 32.20936967632027 38.0 31.0 38.0 11.0 38.0 24-25 32.23859625212947 38.0 31.0 38.0 11.0 38.0 26-27 32.185935264054514 38.0 31.0 38.0 11.0 38.0 28-29 32.25645655877342 38.0 31.0 38.0 11.0 38.0 30-31 32.25649744463373 38.0 31.0 38.0 11.0 38.0 32-33 32.32966269165247 38.0 31.0 38.0 11.0 38.0 34-35 32.27249744463373 38.0 31.0 38.0 11.0 38.0 36-37 32.22299829642249 38.0 31.0 38.0 11.0 38.0 38-39 32.20015672913118 38.0 31.0 38.0 11.0 38.0 40-41 32.26324361158433 38.0 31.0 38.0 11.0 38.0 42-43 32.34238500851789 38.0 31.0 38.0 11.0 38.0 44-45 32.41691311754685 38.0 31.0 38.0 11.0 38.0 46-47 32.3283475298126 38.0 31.5 38.0 11.0 38.0 48-49 32.27553662691652 38.0 31.0 38.0 11.0 38.0 50-51 32.237948892674616 38.0 31.0 38.0 11.0 38.0 52-53 32.33249744463373 38.0 31.0 38.0 11.0 38.0 54-55 32.24263032367973 38.0 31.0 38.0 11.0 38.0 56-57 32.19681771720613 38.0 31.0 38.0 11.0 38.0 58-59 32.28243270868825 38.0 31.0 38.0 11.0 38.0 60-61 32.29379897785349 38.0 31.0 38.0 11.0 38.0 62-63 32.13591822827939 38.0 31.0 38.0 11.0 38.0 64-65 32.07403747870528 38.0 31.0 38.0 11.0 38.0 66-67 32.01326064735945 37.0 30.5 38.0 11.0 38.0 68-69 31.881199318568996 37.0 29.5 38.0 11.0 38.0 70-71 31.8282930153322 37.0 29.0 38.0 11.0 38.0 72-73 31.78111073253833 37.0 28.0 38.0 11.0 38.0 74-75 31.765417376490632 37.0 28.0 38.0 11.0 38.0 76 30.276415672913117 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4209672536215514 1101 2 0.6067008878459532 1101 3 0.3434864269064981 1101 4 1.8982205768352571 1101 5 0.7509905603505196 1101 6 1.1067803582805098 1101 7 -0.015216854373210964 1101 8 0.07747754978895927 1101 9 1.229091575426743 1101 10-11 0.1565866134733369 1101 12-13 0.7175506924861352 1101 14-15 0.21733777118771513 1101 16-17 0.25219254660375867 1101 18-19 0.22852661240733596 1101 20-21 0.7642770585218628 1101 22-23 0.7101595339670723 1101 24-25 -0.0014702857468691377 1101 26-27 -0.13033743714441215 1101 28-29 0.04288203603154983 1101 30-31 0.026482349872253508 1101 32-33 0.4954623568768213 1101 34-35 -0.09575330634437762 1101 36-37 0.6076507528181239 1101 38-39 0.26785026478501095 1101 40-41 -0.1363353091109012 1101 42-43 0.01985115110380775 1101 44-45 0.28742754873175613 1101 46-47 0.11263002734102656 1101 48-49 0.3829130140684356 1101 50-51 0.11713040769059546 1101 52-53 0.3416299399199261 1101 54-55 0.3034010507938305 1101 56-57 0.6574649125574652 1101 58-59 0.6208152602947621 1101 60-61 0.12208366155017103 1101 62-63 -0.48948821414997923 1101 64-65 0.31266346505393017 1101 66-67 0.6251119269806615 1101 68-69 0.26607070971197544 1101 70-71 0.09191199937388816 1101 72-73 0.10855997182799726 1101 74-75 -0.34748618802278486 1101 76 -0.32874670253733385 1102 1 0.6771248890895301 1102 2 -0.1685358461186688 1102 3 -0.639999624294397 1102 4 -0.32100400776363713 1102 5 -1.0003303003146335 1102 6 -0.655132911583447 1102 7 -0.3685694156943278 1102 8 0.21109504895942877 1102 9 -0.21229283969360324 1102 10-11 0.08037231499711339 1102 12-13 -0.3235299884909253 1102 14-15 -0.07496167494414507 1102 16-17 -0.22450415315887184 1102 18-19 -0.7832909429156629 1102 20-21 -0.1384769678698312 1102 22-23 -0.12795527243432048 1102 24-25 -0.43937069157652786 1102 26-27 -0.25388576660662565 1102 28-29 0.06889086729319871 1102 30-31 -0.03062732622974096 1102 32-33 -0.44804054008328364 1102 34-35 -0.5668219910989691 1102 36-37 -0.7516233481032941 1102 38-39 -0.7181498883582549 1102 40-41 -0.8299594444895462 1102 42-43 -0.4867084854361785 1102 44-45 -0.2875743655590597 1102 46-47 -0.30482575254105626 1102 48-49 0.17812218224526077 1102 50-51 0.1408420899693681 1102 52-53 -0.05270120686789426 1102 54-55 -0.6536167393488483 1102 56-57 -0.2195610452260368 1102 58-59 -0.23331577929277358 1102 60-61 -0.5654019812686855 1102 62-63 -0.7840592853871229 1102 64-65 -0.5626268690536307 1102 66-67 -0.08260138882627643 1102 68-69 -0.8626972527168775 1102 70-71 -0.5797233154631414 1102 72-73 -0.9911362940287098 1102 74-75 -0.6596559728564984 1102 76 -0.03256762701218108 1103 1 -0.020436086520227548 1103 2 -0.6336691710270479 1103 3 0.2613525809374977 1103 4 -0.43903762333295404 1103 5 0.6563612199684457 1103 6 -0.2736593870036437 1103 7 0.22399977424627693 1103 8 0.15963542555491017 1103 9 0.39415225066404247 1103 10-11 0.6714250130888573 1103 12-13 0.2287005182701094 1103 14-15 0.10993659372766018 1103 16-17 0.24634129104686764 1103 18-19 0.071239576482796 1103 20-21 0.4435273288570727 1103 22-23 0.1577265170260702 1103 24-25 0.4024465711556857 1103 26-27 -0.22268394681640302 1103 28-29 0.07749695853545546 1103 30-31 0.13479665961633458 1103 32-33 -0.38151709764678543 1103 34-35 -0.17242037350766282 1103 36-37 -0.07787129452058394 1103 38-39 -0.006005316135329508 1103 40-41 0.16158611345308316 1103 42-43 -0.465742985625738 1103 44-45 0.34837912869090815 1103 46-47 0.40001438394320843 1103 48-49 0.5512297267922079 1103 50-51 -0.3185538407775077 1103 52-53 0.3583186339004598 1103 54-55 0.0592102542060573 1103 56-57 0.2133341904620707 1103 58-59 0.47871505604148723 1103 60-61 0.36128827527118546 1103 62-63 -0.009144588053619174 1103 64-65 0.5179620352810019 1103 66-67 0.48275940985943677 1103 68-69 0.16348750654004363 1103 70-71 0.5593516245090555 1103 72-73 0.2985515613990657 1103 74-75 0.20300041536541613 1103 76 0.6281678715345151 1104 1 0.822052425321413 1104 2 0.8649051190636783 1104 3 0.8619652827166888 1104 4 0.6215255909948425 1104 5 0.0997923499319171 1104 6 -0.2506123739248167 1104 7 0.22187888347215434 1104 8 0.4817516032384219 1104 9 0.12124073894022303 1104 10-11 0.30674962720456733 1104 12-13 0.09953827012588334 1104 14-15 0.13987650182239975 1104 16-17 0.21644851385822506 1104 18-19 0.43148465719446705 1104 20-21 0.657961168847649 1104 22-23 0.758480611523531 1104 24-25 0.4241621361374257 1104 26-27 0.2954496693023714 1104 28-29 0.7612014477542672 1104 30-31 0.712032431974059 1104 32-33 0.7874354179653196 1104 34-35 0.4166452562790681 1104 36-37 -0.03033977572713553 1104 38-39 0.6094889692423067 1104 40-41 0.9199341751945695 1104 42-43 0.02664537304045922 1104 44-45 -0.18604999820905732 1104 46-47 0.5997198157801407 1104 48-49 -0.21848866604389272 1104 50-51 0.4015239252175462 1104 52-53 0.38193121785238304 1104 54-55 0.10717773388086016 1104 56-57 0.7681238687936371 1104 58-59 0.6775250006626763 1104 60-61 0.4606048771328588 1104 62-63 0.461917035606092 1104 64-65 0.7579408263732645 1104 66-67 0.8769740911366846 1104 68-69 1.1232730609173274 1104 70-71 1.355922851090881 1104 72-73 0.39549983522963217 1104 74-75 0.02787375105529577 1104 76 0.928910183305458 1105 1 -0.5872981878335466 1105 2 -0.2571916850983449 1105 3 -1.214655861839237 1105 4 -0.7428603250215069 1105 5 -0.5831322134274259 1105 6 -0.2104089179604891 1105 7 -0.8272431901064436 1105 8 -0.7513401842011902 1105 9 -1.578150751212135 1105 10-11 -0.8465158874739949 1105 12-13 -1.7165152415798026 1105 14-15 -1.7167636876982293 1105 16-17 -0.6600611479775225 1105 18-19 -1.1482022621663646 1105 20-21 -0.6678586936569992 1105 22-23 -0.7098977794092107 1105 24-25 -1.3548001455422494 1105 26-27 -1.249700832369875 1105 28-29 -0.9138960996258945 1105 30-31 -1.3112047475131234 1105 32-33 -1.8156048087162056 1105 34-35 -0.8555699982731397 1105 36-37 -1.3098014070909372 1105 38-39 -0.6821544718576575 1105 40-41 -0.49602411168763183 1105 42-43 -0.49874017136682625 1105 44-45 -0.6429201208400492 1105 46-47 -0.7766272670886849 1105 48-49 -1.436739316359759 1105 50-51 -1.1054089064435502 1105 52-53 -0.995855850152072 1105 54-55 -0.9473094456063222 1105 56-57 -0.8515110252977784 1105 58-59 -0.6896479833581353 1105 60-61 -0.8522318935843458 1105 62-63 -0.9874396161962089 1105 64-65 -1.4158677020548254 1105 66-67 -0.8650595394839051 1105 68-69 -1.515534538488101 1105 70-71 -1.255141808886819 1105 72-73 -0.49903286636828526 1105 74-75 -0.7543401099629143 1105 76 -1.293079783249727 1106 1 -0.19079963921235787 1106 2 -0.8567018447500132 1106 3 -0.04221391699163135 1106 4 0.2915192105372739 1106 5 0.023181284395505486 1106 6 0.3428385421265929 1106 7 0.07052531497338421 1106 8 0.6354522686289954 1106 9 0.5440372023437448 1106 10-11 -0.1550245957468519 1106 12-13 0.3108795334564789 1106 14-15 0.4681510437966949 1106 16-17 0.5911180973054968 1106 18-19 0.28585071316020105 1106 20-21 0.46054297542863054 1106 22-23 0.2838885050610358 1106 24-25 1.1830264146899694 1106 26-27 1.0621641894008036 1106 28-29 0.9328927247572736 1106 30-31 -0.03167825984984063 1106 32-33 0.6380871361313964 1106 34-35 0.4515326867776537 1106 36-37 1.1529616553183502 1106 38-39 1.202370201582113 1106 40-41 0.9784406923762248 1106 42-43 0.6002257212311406 1106 44-45 0.8510022303936253 1106 46-47 1.1096209186878028 1106 48-49 0.7792439927839254 1106 50-51 0.4406270151819953 1106 52-53 0.6677871106606972 1106 54-55 0.8870519477173318 1106 56-57 0.6576508034395232 1106 58-59 0.4281858337681328 1106 60-61 0.16178298304845384 1106 62-63 0.38960138525516896 1106 64-65 0.865200875593942 1106 66-67 0.27218646922436207 1106 68-69 0.17582068647561755 1106 70-71 0.8157943304455486 1106 72-73 0.653452619843609 1106 74-75 0.480692546060304 1106 76 0.632355630393235 1107 1 -0.24035083906580823 1107 2 0.2163426688971768 1107 3 0.04127206945277351 1107 4 -0.08822066856146193 1107 5 0.32818220031417056 1107 6 0.30102685500440884 1107 7 0.18895359854180427 1107 8 0.13943651482793484 1107 9 0.2157719522979491 1107 10-11 -0.3734482997219395 1107 12-13 0.14438841786754608 1107 14-15 -0.15293340390435262 1107 16-17 0.24709904619723488 1107 18-19 0.004663682568512684 1107 20-21 0.08160452516301575 1107 22-23 -0.025875187923027454 1107 24-25 0.3126289059887455 1107 26-27 0.2633686224471532 1107 28-29 -0.07413695100752449 1107 30-31 -0.03149507611655622 1107 32-33 0.13176729733308434 1107 34-35 0.5337363870890712 1107 36-37 0.08435934720331062 1107 38-39 -0.19312347857908918 1107 40-41 0.6093483677746576 1107 42-43 -0.19286825202403435 1107 44-45 -0.44561802972727804 1107 46-47 -0.059114197633867605 1107 48-49 0.3246964566051389 1107 50-51 0.051727949419031916 1107 52-53 0.22697835447824843 1107 54-55 0.861223041324223 1107 56-57 0.1413474511772037 1107 58-59 0.13564141171281108 1107 60-61 -0.024491975735209337 1107 62-63 0.2117528782025957 1107 64-65 -0.0010292031750651631 1107 66-67 -0.3276715633823386 1107 68-69 0.3944962680839694 1107 70-71 0.3418522165052664 1107 72-73 -0.18429238839965123 1107 74-75 0.2618566786853336 1107 76 0.22587092623496474 1108 1 0.667223898990521 1108 2 0.10738371474933217 1108 3 0.43466820518284877 1108 4 0.5916312972176385 1108 5 0.28415643851468886 1108 6 0.49784508051627796 1108 7 0.514388569223339 1108 8 0.6521251623334621 1108 9 0.5488008862593077 1108 10-11 0.7270275245595172 1108 12-13 0.24159595415515156 1108 14-15 0.5057467845028327 1108 16-17 0.46927053519079465 1108 18-19 0.6115559251908351 1108 20-21 0.5953505599606714 1108 22-23 0.6105277727613938 1108 24-25 0.6153540814189071 1108 26-27 0.426422549199053 1108 28-29 0.7963571372362566 1108 30-31 0.31186741547240615 1108 32-33 0.9293256939494441 1108 34-35 0.5656742896171849 1108 36-37 0.3218389204033443 1108 38-39 0.38329678480647544 1108 40-41 0.848630305677176 1108 42-43 1.0088283739492425 1108 44-45 0.7973407321683368 1108 46-47 0.32942756916417437 1108 48-49 0.75171270191899 1108 50-51 0.8035543456508876 1108 52-53 0.5695478055981198 1108 54-55 0.7862206381712866 1108 56-57 0.376640155204889 1108 58-59 0.40207897779951907 1108 60-61 1.0910516174590654 1108 62-63 0.8694958369644894 1108 64-65 0.6824995865971175 1108 66-67 0.44487001116347 1108 68-69 0.6943616915195179 1108 70-71 0.9005131796889643 1108 72-73 0.9317078038525786 1108 74-75 0.7655094711490449 1108 76 0.4431159517083856 1109 1 0.17712488908953006 1109 2 0.013769853363196916 1109 3 0.7347672150838385 1109 4 0.2992550595938752 1109 5 -0.6585168288120435 1109 6 0.4317064666548944 1109 7 -5.124208756726034E-4 1109 8 -0.026340184201188777 1109 9 0.31281078724940414 1109 10-11 0.46502257406447 1109 12-13 -0.5695921646567257 1109 14-15 -0.008782918467460377 1109 16-17 -0.36756114797752204 1109 18-19 0.17910543014132685 1109 20-21 -0.24257023211853834 1109 22-23 0.36077529751387516 1109 24-25 -0.20249245323455511 1109 26-27 0.2116453214762828 1109 28-29 -0.02158840731819822 1109 30-31 0.05869909864072298 1109 32-33 -0.10243173179313203 1109 34-35 0.05087230941916232 1109 36-37 -0.3383590993986303 1109 38-39 0.21515322045003415 1109 40-41 0.11561050369698478 1109 42-43 0.11145213632547524 1109 44-45 0.49496449454456837 1109 46-47 0.07702657906516208 1109 48-49 0.01787606825562449 1109 50-51 0.10199493971029483 1109 52-53 -0.8152789270751484 1109 54-55 -0.21865559945247526 1109 56-57 0.09156589777914803 1109 58-59 0.5455443243341733 1109 60-61 0.015556567954114087 1109 62-63 -0.19176653927313225 1109 64-65 -0.05173308667021104 1109 66-67 0.15647892205455705 1109 68-69 0.22129238458882128 1109 70-71 0.11947357572856987 1109 72-73 0.08769790286248025 1109 74-75 0.282390659267854 1109 76 0.09999713982719527 1110 1 -0.06659370387529506 1110 2 0.0014331699461109793 1110 3 0.025269727646655582 1110 4 -0.43707659869757975 1110 5 -0.3706273815758614 1110 6 -0.2601528298275184 1110 7 0.8838845640489517 1110 8 -0.2676718424926463 1110 9 0.125197721922774 1110 10-11 -0.06996486312145578 1110 12-13 0.5161867298156366 1110 14-15 -0.139272868216203 1110 16-17 0.377313223881778 1110 18-19 -0.1383694442305341 1110 20-21 -0.32865063412858575 1110 22-23 -0.1390990743454239 1110 24-25 -0.2607964733350592 1110 26-27 0.022235773737584452 1110 28-29 0.3358864670536619 1110 30-31 -0.03084864005274568 1110 32-33 0.37516877071943355 1110 34-35 0.16773160590157943 1110 36-37 -0.1055952803031559 1110 38-39 -0.21817592527860796 1110 40-41 -0.34310808926784375 1110 42-43 0.11328630717975585 1110 44-45 0.22466298700688014 1110 46-47 0.21651150368827388 1110 48-49 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2116 26-27 0.9311723485033099 2116 28-29 -0.03206138029117511 2116 30-31 0.4732936932353198 2116 32-33 0.5172304303690325 2116 34-35 0.59857501212187 2116 36-37 0.26326252222299473 2116 38-39 0.5125180853149018 2116 40-41 -0.1534435503570748 2116 42-43 -0.1265208366474937 2116 44-45 -0.06638685680678691 2116 46-47 0.35195901149759123 2116 48-49 0.7448355277150824 2116 50-51 0.9081435883589464 2116 52-53 0.07789674860052997 2116 54-55 0.36033088703401717 2116 56-57 -0.040258426545179304 2116 58-59 0.1893281081179552 2116 60-61 0.08515116254870492 2116 62-63 0.6470848120782193 2116 64-65 0.0436047511676243 2116 66-67 0.2934383815140116 2116 68-69 0.5026437359401683 2116 70-71 0.6490681703231616 2116 72-73 0.8218870920516714 2116 74-75 0.3354987673759666 2116 76 0.33337551820557465 2117 1 0.5718617311947938 2117 2 -0.22713657938534126 2117 3 0.1528373905224356 2117 4 0.2912433636874425 2117 5 0.6245825863926342 2117 6 0.15849009238588962 2117 7 0.4255402107032715 2117 8 0.5483674181379925 2117 9 0.7764657580096355 2117 10-11 0.6965284220176855 2117 12-13 0.3691797651678357 2117 14-15 0.4528691283161663 2117 16-17 0.5661815420809617 2117 18-19 0.628535254702733 2117 20-21 0.10965199010368565 2117 22-23 0.12817295833258413 2117 24-25 -0.026893038029875527 2117 26-27 0.40414532147627824 2117 28-29 0.08652562776951811 2117 30-31 0.6535528998103146 2117 32-33 0.26150101674488013 2117 34-35 0.6438255257934316 2117 36-37 0.19836604680019576 2117 38-39 0.8073023432570494 2117 40-41 0.21639997738119376 2117 42-43 0.2201363468517954 2117 44-45 0.18554928986620212 2117 46-47 0.8049066960242257 2117 48-49 0.17777372907433886 2117 50-51 0.42733119702023714 2117 52-53 0.45426785655057955 2117 54-55 0.5311543420679925 2117 56-57 0.5244167749721313 2117 58-59 -0.01609310256640839 2117 60-61 0.551375281404404 2117 62-63 0.8085989578028858 2117 64-65 -0.1310751919333697 2117 66-67 0.46380348345806155 2117 68-69 -0.40660235225328023 2117 70-71 0.09073088567008725 2117 72-73 -0.6000506351492128 2117 74-75 0.44727370020353163 2117 76 -0.812254322161106 2118 1 -0.8645417775771378 2118 2 -0.47765871806537774 2118 3 -0.5752327849161603 2118 4 -0.188602083263266 2118 5 -0.5794692097644223 2118 6 -0.41210305715463136 2118 7 0.2961542457909907 2118 8 -0.2751497080107157 2118 9 -0.5367130222744052 2118 10-11 -0.837358378316484 2118 12-13 -0.5205445456091056 2118 14-15 0.10752660534206626 2118 16-17 -0.8922040051203801 2118 18-19 -0.18506123652533546 2118 20-21 -0.5976892797375832 2118 22-23 -0.390415178676605 2118 24-25 0.07857897533687463 2118 26-27 0.21962151195246804 2118 28-29 0.44210206887227343 2118 30-31 0.11977052721215387 2118 32-33 0.3639968396354405 2118 34-35 -0.007699119152263734 2118 36-37 -0.01847814701768158 2118 38-39 0.25122464902146646 2118 40-41 0.44822955131603237 2118 42-43 -0.34580976843642475 2118 44-45 0.04984544692551651 2118 46-47 -0.2965448495062688 2118 48-49 0.2365665444460987 2118 50-51 0.43270922542458123 2118 52-53 0.35281631102009214 2118 54-55 0.5625348767380061 2118 56-57 0.6548992311124735 2118 58-59 -0.13195567566582866 2118 60-61 0.06317561557316509 2118 62-63 0.11180488929829835 2118 64-65 0.12362405618692662 2118 66-67 0.21552654110217517 2118 68-69 -0.07311237731594034 2118 70-71 -0.09076451950952702 2118 72-73 0.35650742667200674 2118 74-75 0.8958430402202353 2118 76 -0.2450028601728036 2119 1 0.10316039204811034 2119 2 -0.04010588628177558 2119 3 -1.152155861839237 2119 4 -0.9577271889268353 2119 5 0.021897372371388002 2119 6 -0.0658083262445146 2119 7 -0.33870768714786337 2119 8 0.3832752004141966 2119 9 0.4170415564801715 2119 10-11 0.7529663610467168 2119 12-13 0.02017114895274119 2119 14-15 0.14806619395857723 2119 16-17 0.11545660350176945 2119 18-19 -0.025051374592401032 2119 20-21 -0.06227437413036796 2119 22-23 -0.5843430456813934 2119 24-25 -0.25975576684402313 2119 26-27 -0.06035171994383859 2119 28-29 -0.1531712475548872 2119 30-31 -0.1360938007675614 2119 32-33 0.09175465873941846 2119 34-35 -0.2485063888056871 2119 36-37 0.1286231491220846 2119 38-39 -0.19140003398783278 2119 40-41 -0.4129989637586391 2119 42-43 0.009765745792940095 2119 44-45 -0.08485799066253463 2119 46-47 -0.01479294756205718 2119 48-49 -0.10278961221774807 2119 50-51 -0.28606719046721807 2119 52-53 -0.01730555429408298 2119 54-55 -0.16994258170100096 2119 56-57 -0.5064666465995558 2119 58-59 -0.3630947289202666 2119 60-61 -0.07098189358434581 2119 62-63 -0.4007162434151468 2119 64-65 -0.44157036477672307 2119 66-67 -0.6553701903714817 2119 68-69 -0.9853940059437214 2119 70-71 -0.5532187319637387 2119 72-73 0.07530145315833892 2119 74-75 -0.2820767963534436 2119 76 -0.08523954656333643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 190.0 14 794.0 15 1382.0 16 1922.0 17 2309.0 18 2453.0 19 2119.0 20 1653.0 21 1266.0 22 908.0 23 746.0 24 657.0 25 619.0 26 659.0 27 734.0 28 845.0 29 917.0 30 1185.0 31 1464.0 32 1868.0 33 2412.0 34 3207.0 35 4788.0 36 8953.0 37 29312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.099148211243616 20.46201022146508 11.689267461669505 32.749574105621804 2 18.23236797274276 19.652470187393526 34.811584327086884 27.303577512776833 3 17.62862010221465 24.11039182282794 28.620102214650768 29.640885860306643 4 14.31686541737649 19.493015332197615 30.048381601362863 36.14173764906303 5 14.979216354344121 31.17955706984668 30.784327086882453 23.056899488926746 6 26.721635434412267 34.202385008517886 17.501873935264054 21.574105621805792 7 25.606814310051107 26.402725724020442 22.52810902896082 25.46235093696763 8 22.50221465076661 29.845315161839864 22.518568994889268 25.13390119250426 9 22.096081771720613 17.854855195911416 21.420102214650765 38.62896081771721 10-11 24.551277683134582 23.334241908006813 25.734241908006815 26.38023850085179 12-13 23.489608177172062 20.902896081771722 25.529812606473595 30.07768313458262 14-15 23.09982964224872 22.197614991482112 23.365587734241906 31.336967632027257 16-17 23.90732538330494 23.836456558773424 23.76831345826235 28.487904599659288 18-19 22.978010071618886 22.784482347409522 24.82401924374272 29.41348833722887 20-21 22.982623509369677 23.17955706984668 25.013287904599657 28.82453151618399 22-23 24.867461669505964 22.620102214650768 23.992504258943782 28.51993185689949 24-25 23.58977853492334 24.41771720613288 23.08006814310051 28.91243611584327 26-27 22.703236797274275 24.59148211243612 24.654855195911413 28.050425894378193 28-29 23.447359454855196 23.72402044293015 25.863713798977855 26.964906303236795 30-31 23.47870528109029 22.95127768313458 26.088586030664395 27.481431005110736 32-33 22.303236797274277 24.3045996592845 25.882793867120956 27.509369676320272 34-35 22.572402044293018 23.743100511073255 26.207836456558773 27.476660988074958 36-37 22.8824531516184 22.67052810902896 26.51243611584327 27.934582623509367 38-39 21.736286201022146 22.616013628620102 28.663713798977852 26.9839863713799 40-41 21.64770017035775 22.28960817717206 28.75298126064736 27.30971039182283 42-43 20.217376490630322 22.63168654173765 28.53083475298126 28.620102214650768 44-45 19.70902896081772 22.24395229982964 29.800340715502553 28.246678023850087 46-47 20.014310051107326 22.924020442930154 29.941396933560476 27.120272572402044 48-49 19.521635434412264 23.438500851788756 29.465076660988075 27.5747870528109 50-51 19.464395229982966 24.515843270868825 27.883475298126065 28.13628620102215 52-53 18.487904599659284 24.385689948892676 28.397274275979555 28.729131175468485 54-55 18.190800681431003 23.982964224872234 28.703918228279385 29.122316865417375 56-57 19.104599659284496 24.164906303236798 27.6763202725724 29.054173764906306 58-59 19.210902896081773 24.303918228279386 27.08756388415673 29.39761499148211 60-61 18.823850085178876 23.317887563884156 27.616354344122655 30.24190800681431 62-63 19.501192504258942 23.32947189097104 27.184327086882455 29.98500851788756 64-65 20.02998296422487 23.35536626916525 26.082453151618402 30.532197614991478 66-67 19.44190800681431 24.713458262350937 24.932197614991484 30.912436115843274 68-69 20.02793867120954 25.001703577512778 24.53219761499148 30.438160136286204 70-71 20.83747870528109 24.07155025553663 24.909028960817718 30.181942078364564 72-73 20.83747870528109 23.026235093696762 26.43884156729131 29.697444633730836 74-75 21.167972742759797 23.03304940374787 26.776831345826235 29.022146507666097 76 20.981546596887178 22.289911957914246 26.768337558263145 29.960203886935428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.5 14 2.0 15 4.0 16 7.0 17 9.0 18 9.5 19 12.5 20 15.0 21 15.0 22 22.0 23 51.5 24 81.5 25 89.0 26 124.0 27 195.0 28 292.5 29 354.0 30 406.5 31 547.0 32 758.5 33 882.0 34 1032.5 35 1328.5 36 1641.0 37 1808.0 38 1990.5 39 2428.0 40 2971.0 41 3400.0 42 3541.0 43 3625.0 44 3874.5 45 4083.0 46 4126.0 47 4131.0 48 3963.0 49 3538.5 50 3287.0 51 3204.0 52 2838.5 53 2337.5 54 2119.0 55 2061.5 56 1875.0 57 1740.5 58 1735.0 59 1716.0 60 1782.5 61 1915.0 62 1962.0 63 1978.5 64 2015.0 65 1996.5 66 1844.5 67 1731.0 68 1588.5 69 1315.5 70 1025.5 71 866.0 72 699.5 73 410.0 74 228.0 75 169.0 76 112.0 77 39.5 78 16.5 79 9.0 80 9.0 81 8.5 82 9.5 83 11.0 84 7.0 85 4.5 86 5.0 87 4.0 88 4.0 89 5.5 90 5.5 91 4.0 92 4.0 93 3.0 94 2.0 95 1.5 96 1.0 97 1.5 98 3.5 99 13.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.814310051107325E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.001362862010221465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 73375.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.83713798977853 #Duplication Level Percentage of deduplicated Percentage of total 1 79.00005854458169 55.17137989778534 2 11.945046152645238 16.684156729131175 3 4.197646507815701 8.794548551959114 4 1.9651464590285501 5.489608177172061 5 1.077220303260933 3.7614991482112434 6 0.6459418847452335 2.7066439522998293 7 0.40590909977948214 1.9843270868824532 8 0.2614991315887048 1.4609880749574107 9 0.13465253790761664 0.8463373083475297 >10 0.36687937864683956 3.1005110732538332 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.014991482112436116 0.0 2 0.0 0.0 0.0 0.016354344122657582 0.0 3 0.0 0.0 0.0 0.016354344122657582 0.0 4 0.0 0.0 0.0 0.05042589437819421 0.0 5 0.0 0.0 0.0 0.054514480408858604 0.0 6 0.0 0.0 0.0 0.054514480408858604 0.0 7 0.0 0.0 0.0 0.055877342419080066 0.0 8 0.0 0.0 0.0 0.057240204429301535 0.0 9 0.0 0.0 0.0 0.06269165247018739 0.0 10 0.0 0.0 0.0 0.06541737649063033 0.0 11 0.0 0.0 0.0 0.06541737649063033 0.0 12 0.0 0.0 0.0 0.06950596252129472 0.0 13 0.0 0.0 0.0 0.07223168654173764 0.0 14 0.0 0.0 0.0 0.07223168654173764 0.0 15 0.0 0.0 0.0 0.07495741056218058 0.0 16 0.0 0.0 0.0 0.07632027257240205 0.0 17 0.0 0.0 0.0 0.07904599659284497 0.0 18 0.0 0.0 0.0 0.08313458262350937 0.0 19 0.0 0.0 0.0 0.09540034071550256 0.0 20 0.0 0.0 0.0 0.10221465076660988 0.0 21 0.0 0.0 0.0 0.10494037478705281 0.0 22 0.0 0.0 0.0 0.11039182282793868 0.0 23 0.0 0.0 0.0 0.11448040885860307 0.0 24 0.0 0.0 0.0 0.11993185689948893 0.0 25 0.0 0.0 0.0 0.1321976149914821 0.0 26 0.0 0.0 0.0 0.14582623509369677 0.0 27 0.0 0.0 0.0 0.24258943781942077 0.0 28 0.0 0.0 0.0 0.3706984667802385 0.0 29 0.0 0.0 0.0 0.4797274275979557 0.0 30 0.0 0.0 0.0 0.6378194207836456 0.0 31 0.0 0.0 0.0 0.782282793867121 0.0 32 0.0 0.0 0.0 0.9526405451448041 0.0 33 0.0 0.0 0.0 1.1979557069846678 0.0 34 0.0 0.0 0.0 1.5182282793867121 0.0 35 0.0 0.0 0.0 1.9597955706984669 0.0 36 0.0 0.0 0.0 2.603066439522998 0.0 37 0.0 0.0 0.0 3.4017035775127766 0.0 38 0.0 0.0 0.0 4.218057921635435 0.0 39 0.0 0.0 0.0 5.015332197614992 0.0 40 0.0 0.0 0.0 5.873935264054515 0.0 41 0.0 0.0 0.0 6.697103918228279 0.0 42 0.0 0.0 0.0 7.537989778534923 0.0 43 0.0 0.0 0.0 8.367972742759795 0.0 44 0.0 0.0 0.0 9.230664395229983 0.0 45 0.0 0.0 0.0 10.139693356047701 0.0 46 0.0 0.0 0.0 11.033730834752982 0.0 47 0.0 0.0 0.0 11.927768313458262 0.0 48 0.0 0.0 0.0 12.752299829642249 0.0 49 0.0 0.0 0.0 13.504599659284498 0.0 50 0.0 0.0 0.0 14.22419080068143 0.0 51 0.0 0.0 0.0 14.9396933560477 0.0 52 0.0 0.0 0.0 15.63747870528109 0.0 53 0.0 0.0 0.0 16.350255536626918 0.0 54 0.0 0.0 0.0 17.084838160136286 0.0 55 0.0 0.0 0.0 17.736286201022146 0.0 56 0.0 0.0 0.0 18.368654173764906 0.0 57 0.0 0.0 0.0 19.06098807495741 0.0 58 0.0 0.0 0.0 19.67427597955707 0.0 59 0.0 0.0 0.0 20.248040885860306 0.0 60 0.0 0.0 0.0 20.81090289608177 0.0 61 0.0 0.0 0.0 21.44190800681431 0.0 62 0.0 0.0 0.0 22.018398637137988 0.0 63 0.0 0.0 0.0 22.609880749574106 0.0 64 0.0 0.0 0.0 23.142759795570697 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCCGA 15 0.002216587 70.0 18 AAAGATA 15 0.002216587 70.0 25 GGTGGTA 20 0.0069262474 52.500004 47 TAACAGA 20 0.0069262474 52.500004 44 ACGGATG 20 0.0069262474 52.500004 8 ACCAGTG 20 0.0069262474 52.500004 25 GTACTGG 20 0.0069262474 52.500004 19 TGAGATT 20 0.0069262474 52.500004 53 CGAACAT 30 5.8432814E-4 46.666664 25 TCTCGGT 60 4.833055E-9 46.666664 43 GACGACC 50 3.4363748E-6 42.000004 23 TATACCC 35 0.0012480996 40.000004 3 AGAGAAA 35 0.0012480996 40.000004 37 CGACGAA 55 6.613898E-6 38.18182 22 GTGGGAG 40 0.0024047468 35.000004 40 ATATCCT 40 0.0024047468 35.000004 49 ACGCTTC 40 0.0024047468 35.000004 15 ATCTCGG 60 1.2011204E-5 35.0 42 TCGGTGG 75 1.4667748E-6 32.666668 45 AGATCTC 65 2.0774542E-5 32.307693 40 >>END_MODULE