FastQCFastQC Report
Wed 25 May 2016
SRR1780254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73375
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT69329.447359454855196No Hit
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG20412.78160136286201RNA PCR Primer, Index 23 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC19022.5921635434412265No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG18822.564906303236797No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC3900.5315161839863713No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2720.3706984667802385RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT1710.23304940374787053No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1180.1608177172061329No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1000.1362862010221465No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT980.1335604770017036No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT840.11448040885860306No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG830.11311754684838161No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGAAT150.00221658770.019
TTTACAC150.00221658770.02
CAGCATC150.00221658770.04
ATAGCGA150.00221658770.039
TTACTCG150.00221658770.019
GGATAGC150.00221658770.037
AATATGC150.00221658770.067
AGCGGAG150.00221658770.056
TTTGACG150.00221658770.023
GTTGGAA150.00221658770.018
AGGTGAA150.00221658770.061
AAAACCT252.3762583E-456.00000468
ATTACTC200.006926247452.50000418
ATGCTCG200.006926247452.50000470
ATATGCT200.006926247452.50000468
TATGCTC200.006926247452.50000469
GCCATTA200.006926247452.50000427
GAGCGTT200.006926247452.5000049
AGTTGGA200.006926247452.50000417
TACTCGC200.006926247452.50000420
Produced by FastQC (version 0.11.5)