##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780254_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73375 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.7243339011925 34.0 34.0 34.0 34.0 34.0 2 33.737758091993186 34.0 34.0 34.0 34.0 34.0 3 33.75450766609881 34.0 34.0 34.0 34.0 34.0 4 33.76681431005111 34.0 34.0 34.0 34.0 34.0 5 33.75517546848381 34.0 34.0 34.0 34.0 34.0 6 37.52340715502555 38.0 38.0 38.0 37.0 38.0 7 37.514971039182285 38.0 38.0 38.0 37.0 38.0 8 37.44700511073254 38.0 38.0 38.0 37.0 38.0 9 37.46063373083475 38.0 38.0 38.0 37.0 38.0 10-11 37.41989097103918 38.0 38.0 38.0 37.0 38.0 12-13 37.39189778534923 38.0 38.0 38.0 37.0 38.0 14-15 37.31858262350937 38.0 38.0 38.0 36.5 38.0 16-17 37.35219761499148 38.0 38.0 38.0 36.5 38.0 18-19 37.28825212947189 38.0 38.0 38.0 36.0 38.0 20-21 37.316402044293014 38.0 38.0 38.0 36.5 38.0 22-23 37.32506303236797 38.0 38.0 38.0 36.0 38.0 24-25 37.31499829642249 38.0 38.0 38.0 36.0 38.0 26-27 37.22327768313458 38.0 38.0 38.0 36.0 38.0 28-29 37.15726064735945 38.0 38.0 38.0 36.0 38.0 30-31 37.116 38.0 38.0 38.0 36.0 38.0 32-33 37.01823509369676 38.0 38.0 38.0 36.0 38.0 34-35 37.12271209540034 38.0 38.0 38.0 36.0 38.0 36-37 37.10485860306644 38.0 38.0 38.0 36.0 38.0 38-39 36.30363202725724 38.0 37.0 38.0 34.0 38.0 40-41 36.7076933560477 38.0 37.0 38.0 34.5 38.0 42-43 37.04497444633731 38.0 38.0 38.0 36.0 38.0 44-45 37.135216354344124 38.0 38.0 38.0 36.0 38.0 46-47 37.07595911413969 38.0 38.0 38.0 36.0 38.0 48-49 37.08603066439523 38.0 38.0 38.0 36.0 38.0 50-51 36.9314003407155 38.0 38.0 38.0 35.0 38.0 52-53 36.920415672913116 38.0 38.0 38.0 35.0 38.0 54-55 36.89644293015332 38.0 37.5 38.0 35.5 38.0 56-57 36.95646337308348 38.0 38.0 38.0 35.5 38.0 58-59 36.80715502555366 38.0 37.5 38.0 35.0 38.0 60-61 36.80291652470187 38.0 37.0 38.0 35.0 38.0 62-63 36.79918909710392 38.0 37.0 38.0 35.0 38.0 64-65 36.63649744463373 38.0 37.5 38.0 34.5 38.0 66-67 36.17037819420784 38.0 37.0 38.0 33.5 38.0 68-69 36.0074139693356 38.0 37.0 38.0 33.0 38.0 70-71 35.05980238500852 38.0 36.5 38.0 27.0 38.0 72-73 33.44578534923339 38.0 34.5 38.0 16.0 38.0 74-75 32.95887563884156 38.0 34.0 38.0 11.0 38.0 76 32.401553662691654 38.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0339982591219794 1101 2 -0.12562536182182527 1101 3 -0.2920178535450688 1101 4 -0.3202902051575265 1101 5 -0.30578444143414174 1101 6 -0.3431837498132779 1101 7 -0.20317728958271886 1101 8 0.013312732704022778 1101 9 0.06510896394933496 1101 10-11 0.024918338386207495 1101 12-13 -0.018124783097874797 1101 14-15 -0.004006633445946761 1101 16-17 0.17258340857235765 1101 18-19 0.04143235391594402 1101 20-21 -0.2962706641963706 1101 22-23 -0.18823738135444756 1101 24-25 -0.058594383958535445 1101 26-27 -0.015938559352186132 1101 28-29 -0.3956188171547126 1101 30-31 -0.3948461916962387 1101 32-33 -0.28296695254186943 1101 34-35 -0.29435182201017085 1101 36-37 -0.3275983729024219 1101 38-39 0.42063574879967547 1101 40-41 0.08197412707925622 1101 42-43 -0.19414359501593736 1101 44-45 -0.22030113694194142 1101 46-47 -0.13582740035676721 1101 48-49 -0.289311228787426 1101 50-51 -0.012819066801604606 1101 52-53 0.02979976386643557 1101 54-55 -0.23071181308296929 1101 56-57 -0.18317196448207795 1101 58-59 -0.0220501652489844 1101 60-61 -0.5434409931058894 1101 62-63 -0.03752657034375062 1101 64-65 -0.6050269992887465 1101 66-67 -0.724180345805415 1101 68-69 -0.37008715182788876 1101 70-71 -0.6932303259780355 1101 72-73 0.5556444686473299 1101 74-75 0.3676302503001594 1101 76 -0.3438627854339984 1102 1 0.0747845071558757 1102 2 0.16118645062872616 1102 3 -0.02399161499891278 1102 4 0.07157788744309812 1102 5 -0.08515604356794171 1102 6 -0.14151959299456962 1102 7 0.011325377662487313 1102 8 -0.2582868487043868 1102 9 0.14321202936703514 1102 10-11 -0.04940719314854647 1102 12-13 0.16291608063013996 1102 14-15 0.11995007805766278 1102 16-17 0.1837118192004965 1102 18-19 0.2535102527903348 1102 20-21 0.09012255666173985 1102 22-23 -0.11619623686377878 1102 24-25 -0.007648724293915166 1102 26-27 0.014485902732083389 1102 28-29 0.316203842943807 1102 30-31 -0.04929117497810154 1102 32-33 0.31269654065601316 1102 34-35 -0.4134972825885441 1102 36-37 -0.06952644661537732 1102 38-39 0.2083026111494064 1102 40-41 0.22180669044228551 1102 42-43 -0.18638433622934514 1102 44-45 0.058126081721120215 1102 46-47 0.07651849923230003 1102 48-49 0.32975766015817953 1102 50-51 -0.1153484830705267 1102 52-53 0.26469600930403914 1102 54-55 0.06009448621002633 1102 56-57 -0.1167715969382428 1102 58-59 0.08746003154011817 1102 60-61 0.265078877232817 1102 62-63 -0.1302165318026951 1102 64-65 0.31632117192543063 1102 66-67 0.42688272369610303 1102 68-69 -0.09444203582187072 1102 70-71 -0.40040764035563825 1102 72-73 -1.160530012579862 1102 74-75 -0.4959318620559543 1102 76 -0.883488605388564 1103 1 -0.08960320926015442 1103 2 -0.175727488458854 1103 3 -0.16492425748595707 1103 4 0.08007024179669742 1103 5 0.12389235779690466 1103 6 0.10860046261012712 1103 7 0.21789684234086337 1103 8 0.023477336813115812 1103 9 0.050402728217029846 1103 10-11 0.0033035360312752005 1103 12-13 -0.0853841847559309 1103 14-15 0.11704346868069138 1103 16-17 -0.07390223362396853 1103 18-19 -0.12728085045748116 1103 20-21 -0.07926958285550967 1103 22-23 -0.1652168358780557 1103 24-25 -0.2613578106075778 1103 26-27 -0.0836184824568349 1103 28-29 -0.11550347412936901 1103 30-31 0.12740540027780867 1103 32-33 0.21706909215355807 1103 34-35 -0.18021028650614568 1103 36-37 -0.08643533073012577 1103 38-39 -0.05206640186714395 1103 40-41 -0.3204528824125177 1103 42-43 0.12195636893936523 1103 44-45 0.10239500627564979 1103 46-47 -0.19873740876720092 1103 48-49 -0.20197493177913373 1103 50-51 -0.38278182061633714 1103 52-53 -0.40613679746962106 1103 54-55 -0.517663633340085 1103 56-57 -0.4000649117366706 1103 58-59 -0.14413354864439043 1103 60-61 -0.1210452223501548 1103 62-63 -0.18641519223489667 1103 64-65 -0.02972963055150757 1103 66-67 -0.040917099379853994 1103 68-69 -0.37662325659781004 1103 70-71 -0.031293524344008006 1103 72-73 0.28512902950278374 1103 74-75 0.10633023828525268 1103 76 0.5001838323720804 1104 1 -0.04268439224589571 1104 2 -0.32147981554734173 1104 3 0.05633178116138282 1104 4 0.047573482046487925 1104 5 0.01869617540029367 1104 6 0.025084497015690488 1104 7 0.06078596688428206 1104 8 -0.4013370852441511 1104 9 0.07462801394814988 1104 10-11 -0.18939117352451262 1104 12-13 -0.18757577188919328 1104 14-15 0.013532216176862732 1104 16-17 0.08051039745886612 1104 18-19 0.22417431626809758 1104 20-21 -0.15148591371447395 1104 22-23 -0.22959765359927786 1104 24-25 -0.10029972176582191 1104 26-27 -0.26834806888314233 1104 28-29 -0.17413432130740603 1104 30-31 -0.05125608198918741 1104 32-33 -0.29980377222725707 1104 34-35 0.03800580869828707 1104 36-37 -0.040753700000777826 1104 38-39 -0.08809797957422916 1104 40-41 -0.3237991767450268 1104 42-43 -0.15742385964552597 1104 44-45 -0.02317351027908643 1104 46-47 -0.1469051947928861 1104 48-49 0.006862113113044188 1104 50-51 -0.3180593038259474 1104 52-53 -0.06311806293445699 1104 54-55 -0.22902218170818145 1104 56-57 -0.315665241653015 1104 58-59 -0.08511387149109595 1104 60-61 -0.18748801721288544 1104 62-63 -0.16092915476582448 1104 64-65 -0.07424477135509022 1104 66-67 -0.19694396399632552 1104 68-69 -0.4458349921934186 1104 70-71 -5.05259939622249E-4 1104 72-73 0.39423707710500366 1104 74-75 -0.055717599584426125 1104 76 0.22164017737031116 1105 1 -0.3729287252076219 1105 2 -0.2130164190404713 1105 3 -0.00530887287057169 1105 4 -0.08142131542656728 1105 5 0.07262594128658151 1105 6 -0.03530197641426014 1105 7 0.11557507873861539 1105 8 -0.23327688457525397 1105 9 -0.3124725063045446 1105 10-11 -0.0030989029931092205 1105 12-13 -0.06846345304861501 1105 14-15 0.08632114222665876 1105 16-17 0.18184354498766453 1105 18-19 0.11371352102469245 1105 20-21 0.18432094247282294 1105 22-23 0.12569545305252205 1105 24-25 0.045173615283601976 1105 26-27 0.1694654762673622 1105 28-29 0.07341538140534709 1105 30-31 -0.09242339897172513 1105 32-33 0.201496859508147 1105 34-35 0.027751720998544727 1105 36-37 -0.18290578101154864 1105 38-39 -0.8414308483962287 1105 40-41 -0.13794819198099617 1105 42-43 -0.3395703665202987 1105 44-45 -0.07423491867745469 1105 46-47 0.02406981449734502 1105 48-49 -0.2435907854376751 1105 50-51 -0.21044364050376174 1105 52-53 -0.021486234617846378 1105 54-55 0.15480353363927435 1105 56-57 0.1670317111526387 1105 58-59 -0.11732773250930961 1105 60-61 0.19924089397255074 1105 62-63 0.2566781473523534 1105 64-65 -0.060123626799160945 1105 66-67 -0.40123604872319873 1105 68-69 0.09938518617893521 1105 70-71 -0.28220815849034864 1105 72-73 -0.9822841975140975 1105 74-75 -1.44446713507309 1105 76 -1.406007537233922 1106 1 -0.0684113086619007 1106 2 -0.03939522890984648 1106 3 0.07941536515554759 1106 4 -0.1654198640477631 1106 5 -0.03278044478017961 1106 6 0.05903764622724594 1106 7 -0.04779574854730839 1106 8 -0.08160779894389947 1106 9 0.21338017874625592 1106 10-11 0.06175958757292932 1106 12-13 0.09722050921553915 1106 14-15 -0.11052210886187197 1106 16-17 0.09176734760015393 1106 18-19 0.09502701884763098 1106 20-21 0.04067979588356252 1106 22-23 -0.11400882851496164 1106 24-25 0.1298524977219273 1106 26-27 -0.2620929417297333 1106 28-29 -0.13190936460335934 1106 30-31 0.039243872436117044 1106 32-33 -0.09059312597806723 1106 34-35 -0.25522179133817247 1106 36-37 -0.09187711628005246 1106 38-39 -0.0478441285123381 1106 40-41 0.27424339002190123 1106 42-43 0.08581969153485858 1106 44-45 -0.08262570242200695 1106 46-47 -0.2043862812936581 1106 48-49 0.042376195694401986 1106 50-51 0.14147036530174972 1106 52-53 0.20715128352438938 1106 54-55 0.07606623320386774 1106 56-57 -0.28026509581398074 1106 58-59 0.05166718766486156 1106 60-61 0.09882362256471566 1106 62-63 0.05516327071954663 1106 64-65 0.10587782457964323 1106 66-67 0.09332128073979362 1106 68-69 0.7005281469917009 1106 70-71 0.19774467169833088 1106 72-73 0.2782836544452607 1106 74-75 0.23060543386740306 1106 76 -0.08380144144292245 1107 1 -0.07816584245929903 1107 2 0.03987753166901342 1107 3 0.13313399494122535 1107 4 -0.07460652827197833 1107 5 -0.16682514161109907 1107 6 0.032659188634283964 1107 7 0.15109598986632022 1107 8 0.3076118383523081 1107 9 0.3150013547858208 1107 10-11 0.09823604846319967 1107 12-13 0.09992246928148063 1107 14-15 0.0759589316217344 1107 16-17 -0.11889394567701572 1107 18-19 -0.17642837591331784 1107 20-21 0.03371881401874788 1107 22-23 0.13496076298530824 1107 24-25 0.15572720004836071 1107 26-27 0.10269456513965025 1107 28-29 0.17785432838070392 1107 30-31 0.09710703418735278 1107 32-33 0.07509656077775873 1107 34-35 -0.022028898867027635 1107 36-37 -0.13023121043649866 1107 38-39 -0.031334694550075426 1107 40-41 -0.05028949145821571 1107 42-43 0.16484057448045064 1107 44-45 -0.07598529209044358 1107 46-47 0.11007149485581635 1107 48-49 -0.08248641650563826 1107 50-51 -0.11846735222893301 1107 52-53 -0.05418320997258519 1107 54-55 0.0727460280380825 1107 56-57 -0.10887381535968643 1107 58-59 0.06877196876030212 1107 60-61 -0.3983599275360419 1107 62-63 -0.0874452656424225 1107 64-65 -0.03995932508146183 1107 66-67 0.24476843223273903 1107 68-69 0.38229220634323724 1107 70-71 0.3640297055873205 1107 72-73 0.3860643835165263 1107 74-75 -0.02484428219781165 1107 76 0.08353689891245608 1108 1 -0.06518881660137055 1108 2 0.021084875704389106 1108 3 -0.012449009961208901 1108 4 -0.07542360027044026 1108 5 -0.04114024302415231 1108 6 -0.2283712833449556 1108 7 -0.03317777731621874 1108 8 0.1393430697278717 1108 9 -0.2591118817805551 1108 10-11 -0.13614345744856138 1108 12-13 -0.1250847401773285 1108 14-15 -0.26468799714881897 1108 16-17 -0.09714541160029455 1108 18-19 0.16912098484800708 1108 20-21 0.05030056928165294 1108 22-23 -0.04397800467786084 1108 24-25 0.05171874095002238 1108 26-27 -0.054615826093645126 1108 28-29 0.051352357795295234 1108 30-31 0.07406079752484374 1108 32-33 -0.002044648488798373 1108 34-35 -0.037913739778304034 1108 36-37 -0.006901401727468226 1108 38-39 0.03549698861824169 1108 40-41 0.17726506011344867 1108 42-43 0.21600370050179407 1108 44-45 0.02437037454418345 1108 46-47 0.09411455935644142 1108 48-49 -0.030410092368370556 1108 50-51 0.10065213253508176 1108 52-53 0.10125272197012691 1108 54-55 -0.3338674488435842 1108 56-57 0.04817222905057861 1108 58-59 -0.0034187454567558007 1108 60-61 0.2570760044066631 1108 62-63 -0.004055858740564133 1108 64-65 0.11790379132725803 1108 66-67 0.27812419499348806 1108 68-69 -0.16132501945700994 1108 70-71 0.34341065339083343 1108 72-73 0.5382079959360198 1108 74-75 0.4531908999611858 1108 76 1.101608608996088 1109 1 0.10649435171546173 1109 2 0.07390665788260975 1109 3 0.17007574251404378 1109 4 0.14165560765034968 1109 5 0.17108747974812388 1109 6 -0.37530197641426355 1109 7 0.11692123258476528 1109 8 -0.004815346113716146 1109 9 0.14271980138776286 1109 10-11 0.004401097006891064 1109 12-13 0.09278654695138755 1109 14-15 0.23862883453435302 1109 16-17 0.25030508344920577 1109 18-19 0.3308289056400753 1109 20-21 0.2563401732420516 1109 22-23 0.1488685299756014 1109 24-25 0.2306543845143807 1109 26-27 0.15186932242121287 1109 28-29 0.11870384294380898 1109 30-31 0.1659419856436628 1109 32-33 0.03572762873891833 1109 34-35 0.03265556715238915 1109 36-37 0.20911344975768031 1109 38-39 0.3636653054499206 1109 40-41 0.14820565417284826 1109 42-43 -0.03389728959722049 1109 44-45 0.14355354286101374 1109 46-47 0.34839673757426226 1109 48-49 0.16515921456232263 1109 50-51 -0.044001332811454574 1109 52-53 0.4088022269206206 1109 54-55 0.3342266105623608 1109 56-57 0.06780094192186681 1109 58-59 0.3144030367214654 1109 60-61 0.16212550935716052 1109 62-63 0.08138968581388184 1109 64-65 0.04295329627775857 1109 66-67 -0.25469758718472946 1109 68-69 -0.1475378907441467 1109 70-71 0.20029184150965307 1109 72-73 0.01781195633205357 1109 74-75 0.234379018773069 1109 76 0.3209155396891532 1110 1 0.11071545724309573 1110 2 0.007750878988133536 1110 3 0.07427172241354185 1110 4 -0.01450017124412284 1110 5 -0.12069643984986556 1110 6 -0.23379443872581618 1110 7 -0.0656918327418623 1110 8 -0.08803142651572671 1110 9 0.2059610074179119 1110 10-11 -0.07878985776697789 1110 12-13 -0.10568078973203399 1110 14-15 -0.21571789913399186 1110 16-17 0.22815684224317323 1110 18-19 0.022198252373748062 1110 20-21 -0.04989098253684432 1110 22-23 -0.03968674640631065 1110 24-25 0.012425741298301318 1110 26-27 -0.08755278813155343 1110 28-29 -0.10590670982001882 1110 30-31 0.13217314142255532 1110 32-33 0.07625526692986995 1110 34-35 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0.15739774072220314 2115 66-67 -0.07303092051806459 2115 68-69 -0.09281566852192213 2115 70-71 -1.12804149182368 2115 72-73 -1.363854710334607 2115 74-75 -1.2461765367824924 2115 76 -0.8346400158664018 2116 1 -0.4091813239602189 2116 2 -0.03136361238766483 2116 3 -0.09465398721568619 2116 4 -0.17793898694424115 2116 5 -0.24499360133295767 2116 6 -0.167734408846691 2116 7 -0.18091660525306708 2116 8 0.09031978902142157 2116 9 -0.06390182023385904 2116 10-11 0.01075244835823952 2116 12-13 -0.17707831791347672 2116 14-15 0.08335856426408128 2116 16-17 -0.6286814030372767 2116 18-19 -0.17059001327884005 2116 20-21 -0.157713880812004 2116 22-23 0.0010982597053370569 2116 24-25 -0.11481858845859705 2116 26-27 0.223896349448232 2116 28-29 0.10809573483570034 2116 30-31 -0.049193149491472354 2116 32-33 -0.026569668558373394 2116 34-35 -0.15970929771247455 2116 36-37 -0.040818982674757365 2116 38-39 -0.13268604590142985 2116 40-41 0.019692140659337554 2116 42-43 -0.23308647878641153 2116 44-45 -0.1520545652470915 2116 46-47 0.10238322406075184 2116 48-49 -0.0981515962484849 2116 50-51 -0.009677008487130934 2116 52-53 -0.24153561091721798 2116 54-55 0.052672556508298385 2116 56-57 -0.2579423013213713 2116 58-59 -0.12613750381908062 2116 60-61 -0.2586853014536459 2116 62-63 -0.2688805844563831 2116 64-65 0.003493836818300622 2116 66-67 0.23462673713959248 2116 68-69 -0.23192978263603692 2116 70-71 0.31069724691505485 2116 72-73 0.15348763200773163 2116 74-75 0.5005952349892766 2116 76 0.7249695937432108 2117 1 -0.049295121968754074 2117 2 0.040076248525885205 2117 3 -0.2327605147958991 2117 4 0.01782519829362883 2117 5 0.03725707039139792 2117 6 0.11966878381965529 2117 7 0.13528380568418186 2117 8 -0.25768084318973905 2117 9 -0.2726895553373865 2117 10-11 0.09448881630513739 2117 12-13 0.035154968004015075 2117 14-15 0.1419329281016033 2117 16-17 0.02622613608077984 2117 18-19 -0.04114477857044818 2117 20-21 -0.11652532383397585 2117 22-23 0.001777886700743636 2117 24-25 -0.2512023406318207 2117 26-27 -0.0189201512629964 2117 28-29 -0.3943224728456656 2117 30-31 0.1578718102050658 2117 32-33 -0.17547120167043317 2117 34-35 0.13907369580736173 2117 36-37 0.22494678309100635 2117 38-39 -0.4246095483512491 2117 40-41 -0.3012972698037615 2117 42-43 -0.017581500123533544 2117 44-45 0.0870476949077954 2117 46-47 0.24338211769121898 2117 48-49 -0.27739926496983713 2117 50-51 0.0396828777148599 2117 52-53 -0.546972626880553 2117 54-55 0.07163889126410794 2117 56-57 -0.2304885317623473 2117 58-59 -0.03705895158262962 2117 60-61 -0.040272151461543615 2117 62-63 0.236477405112133 2117 64-65 -0.008391732962003573 2117 66-67 0.012056798780186284 2117 68-69 -0.12190923577338708 2117 70-71 0.15090587659737054 2117 72-73 -0.25259740039308554 2117 74-75 0.17209831701867984 2117 76 -0.15949381703599386 2118 1 -0.13826755304644678 2118 2 -0.034902865926916604 2118 3 -0.4375433051050095 2118 4 -0.003582487587742378 2118 5 -0.2817696631090243 2118 6 -0.08292102403331114 2118 7 -0.03712638646285171 2118 8 0.19208941579104533 2118 9 -0.0553754367074788 2118 10-11 0.09511538272117548 2118 12-13 0.10564368980852379 2118 14-15 -0.004109260703742734 2118 16-17 0.0030431786872995303 2118 18-19 -0.33500442769325645 2118 20-21 0.03264969705156773 2118 22-23 0.04636852997560226 2118 24-25 0.07482105118104698 2118 26-27 0.1807978938497783 2118 28-29 0.2090609858009529 2118 30-31 0.10915627135794637 2118 32-33 -0.0560580855467947 2118 34-35 0.26527461477144243 2118 36-37 0.34101821166243695 2118 38-39 -0.043239456454834624 2118 40-41 0.18141993988714233 2118 42-43 -0.0706830038829267 2118 44-45 0.0018868761943480195 2118 46-47 -0.18231754814001988 2118 48-49 -0.018888404485295496 2118 50-51 0.20885581004568365 2118 52-53 0.21832603644443083 2118 54-55 0.5293456581814056 2118 56-57 0.010896180017105905 2118 58-59 0.074522084340515 2118 60-61 -0.04846972873807687 2118 62-63 0.3472230191472221 2118 64-65 0.21414377246824046 2118 66-67 0.3549452699581295 2118 68-69 0.2903192521129938 2118 70-71 0.47672041293822076 2118 72-73 0.08674052776062524 2118 74-75 0.9087837806778225 2118 76 0.31972506349868013 2119 1 0.07122807952610799 2119 2 -0.13387440720615018 2119 3 -0.14362248233803143 2119 4 0.039140814750943775 2119 5 -0.012433230311053478 2119 6 -0.1388522722722456 2119 7 -0.14071190350990292 2119 8 -0.43298102658708615 2119 9 -0.3831086009790994 2119 10-11 -0.2749184296203282 2119 12-13 -0.39742055364033035 2119 14-15 -0.751696609252626 2119 16-17 0.0010003497213944001 2119 18-19 0.014601094989188823 2119 20-21 0.09574845726571368 2119 22-23 0.1269898317507554 2119 24-25 -0.36551425453887987 2119 26-27 -0.1054975414841195 2119 28-29 0.0854346121745806 2119 30-31 -0.7679041682024916 2119 32-33 -0.19791142451551735 2119 34-35 0.00278870324706304 2119 36-37 -0.371700159709782 2119 38-39 -0.46772522709445497 2119 40-41 -0.00998961209933924 2119 42-43 -0.12673752628361257 2119 44-45 -0.1469050370206446 2119 46-47 -0.15215059970383749 2119 48-49 0.022126670183624242 2119 50-51 -0.07009600736766686 2119 52-53 -0.3593190748545254 2119 54-55 0.07714081174578524 2119 56-57 -0.2046398864804999 2119 58-59 -0.03707625321936803 2119 60-61 -0.21533011194460983 2119 62-63 -0.30156889406777054 2119 64-65 -0.477283390112774 2119 66-67 -0.3494609007941989 2119 68-69 -0.8358958789098239 2119 70-71 0.13606107227888486 2119 72-73 0.3736699444977347 2119 74-75 0.5518346400748371 2119 76 -0.950386235458776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 4.0 24 12.0 25 26.0 26 44.0 27 118.0 28 252.0 29 366.0 30 632.0 31 1044.0 32 1617.0 33 2889.0 34 4819.0 35 7741.0 36 10941.0 37 42866.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.431005110732535 15.700170357751277 12.880408858603065 37.98841567291312 2 17.774446337308348 19.001022146507665 38.26098807495741 24.963543441226577 3 16.907666098807496 17.800340715502553 35.074616695059625 30.217376490630322 4 13.937989778534924 26.469505962521296 32.23850085178876 27.354003407155027 5 13.895741056218059 29.34650766609881 41.64497444633731 15.112776831345826 6 26.093356047700173 39.37717206132879 16.65008517887564 17.8793867120954 7 24.907666098807496 24.794548551959114 22.635775127768312 27.662010221465078 8 21.255195911413967 38.64940374787052 15.458943781942077 24.636456558773425 9 20.36252129471891 16.16763202725724 16.38160136286201 47.08824531516184 10-11 24.423168654173764 28.0824531516184 23.855536626916525 23.638841567291312 12-13 23.388756388415676 18.313458262350938 27.390119250425894 30.907666098807496 14-15 20.311413969335604 17.641567291311755 20.81771720613288 41.22930153321976 16-17 27.580238500851788 26.016354344122654 18.41703577512777 27.986371379897783 18-19 30.693015332197614 23.83850085178876 19.03304940374787 26.435434412265757 20-21 23.345144804088587 20.94991482112436 18.984667802385008 36.720272572402045 22-23 21.086882453151617 27.472572402044293 18.71618398637138 32.72436115843271 24-25 27.65451448040886 27.639522998296425 19.909369676320274 24.796592844974448 26-27 31.57069846678024 28.47223168654174 19.155025553662693 20.80204429301533 28-29 22.562180579216353 29.226575809199318 21.22930153321976 26.98194207836457 30-31 22.127427597955705 30.143781942078363 24.714139693356046 23.014650766609883 32-33 32.04020442930153 21.287223168654172 24.423850085178874 22.24872231686542 34-35 25.799659284497444 24.27802385008518 28.156729131175467 21.765587734241908 36-37 28.424531516183986 27.25383304940375 23.119591141396935 21.202044293015334 38-39 21.503918228279385 23.829642248722315 28.556047700170357 26.11039182282794 40-41 20.717546848381602 18.589437819420784 29.725383304940372 30.967632027257242 42-43 27.2442930153322 18.453151618398635 28.47495741056218 25.82759795570698 44-45 20.031345826235096 25.34855195911414 31.951618398637137 22.66848381601363 46-47 25.127768313458258 20.765928449744465 28.469505962521296 25.63679727427598 48-49 24.438160136286204 19.17342419080068 23.920954003407154 32.467461669505965 50-51 21.865076660988077 19.42010221465077 28.89948892674617 29.815332197614993 52-53 16.676660988074957 19.49778534923339 39.10119250425894 24.72436115843271 54-55 17.593185689948893 21.183645655877342 32.85042589437819 28.37274275979557 56-57 23.00511073253833 22.52810902896082 25.51005110732538 28.956729131175468 58-59 18.853151618398638 23.226575809199318 32.59148211243612 25.328790459965926 60-61 23.02350936967632 28.177172061328793 26.079045996592843 22.720272572402045 62-63 17.941396933560476 38.784327086882456 20.314139693356047 22.96013628620102 64-65 16.81158432708688 40.759795570698465 19.55229982964225 22.8763202725724 66-67 16.943100511073254 40.1396933560477 19.580238500851788 23.336967632027257 68-69 16.96899488926746 40.57035775127768 19.5427597955707 22.917887563884157 70-71 17.24701873935264 39.15979557069847 19.777853492333904 23.815332197614993 72-73 17.34787052810903 35.76149914821124 21.179557069846677 25.71107325383305 74-75 18.261669505962523 33.59727427597956 22.133560477001705 26.007495741056218 76 18.04565587734242 34.038841567291314 22.489948892674615 25.42555366269165 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 4.5 17 7.0 18 5.0 19 7.0 20 11.5 21 12.0 22 20.5 23 29.5 24 41.5 25 53.0 26 89.0 27 128.0 28 193.5 29 256.0 30 314.5 31 404.0 32 552.0 33 669.0 34 771.5 35 999.5 36 1195.0 37 1265.0 38 1499.0 39 3506.0 40 6300.0 41 6831.5 42 6342.0 43 5630.0 44 4537.0 45 3913.0 46 3670.0 47 3519.5 48 3244.0 49 2859.0 50 2599.0 51 2398.5 52 1948.0 53 1620.0 54 1542.0 55 1561.5 56 1437.5 57 1373.5 58 1453.0 59 1435.0 60 1524.5 61 1606.0 62 1580.0 63 1648.5 64 1757.0 65 1739.0 66 1609.0 67 1537.0 68 1477.0 69 1251.0 70 974.0 71 863.0 72 692.0 73 397.5 74 201.0 75 128.0 76 83.0 77 22.0 78 4.0 79 2.0 80 2.0 81 1.5 82 1.5 83 2.0 84 1.0 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 73375.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.47836456558774 #Duplication Level Percentage of deduplicated Percentage of total 1 62.71908322098297 27.89642248722317 2 20.722515014094864 18.43407155025554 3 7.602034563059198 10.143781942078364 4 3.4501777178575805 6.138330494037478 5 1.881358009559995 4.1839863713798975 6 1.0969481554112024 2.927427597955707 7 0.7660252481921804 2.385008517887564 8 0.462679249908077 1.64633730834753 9 0.32479470523348447 1.3001703577512778 >10 0.925358499816154 5.4609880749574105 >50 0.024512807942149776 0.8013628620102214 >100 0.012256403971074888 1.2960817717206132 >500 0.0 0.0 >1k 0.009192302978306165 7.938671209540034 >5k 0.003064100992768722 9.447359454855196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 6932 9.447359454855196 No Hit CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG 2041 2.78160136286201 RNA PCR Primer, Index 23 (95% over 24bp) CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 1902 2.5921635434412265 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 1882 2.564906303236797 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 390 0.5315161839863713 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 272 0.3706984667802385 RNA PCR Primer, Index 47 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT 171 0.23304940374787053 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 118 0.1608177172061329 No Hit CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 100 0.1362862010221465 No Hit TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT 98 0.1335604770017036 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT 84 0.11448040885860306 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 83 0.11311754684838161 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016354344122657582 0.0 2 0.0 0.0 0.0 0.019080068143100513 0.0 3 0.0 0.0 0.0 0.019080068143100513 0.0 4 0.0 0.0 0.0 0.06269165247018739 0.0 5 0.0 0.0 0.0 0.06814310051107325 0.0 6 0.0 0.0 0.0 0.06814310051107325 0.0 7 0.0 0.0 0.0 0.06950596252129472 0.0 8 0.0 0.0 0.0 0.07223168654173764 0.0 9 0.0 0.0 0.0 0.0776831345826235 0.0 10 0.0 0.0 0.0 0.0817717206132879 0.0 11 0.0 0.0 0.0 0.0817717206132879 0.0 12 0.0 0.0 0.0 0.0858603066439523 0.0 13 0.0 0.0 0.0 0.08858603066439523 0.0 14 0.0 0.0 0.0 0.08858603066439523 0.0 15 0.0 0.0 0.0 0.09131175468483815 0.0 16 0.0 0.0 0.0 0.09267461669505962 0.0 17 0.0 0.0 0.0 0.09540034071550256 0.0 18 0.0 0.0 0.0 0.09948892674616695 0.0 19 0.0 0.0 0.0 0.1131175468483816 0.0 20 0.0 0.0 0.0 0.11993185689948893 0.0 21 0.0 0.0 0.0 0.12265758091993186 0.0 22 0.0 0.0 0.0 0.12810902896081772 0.0 23 0.0 0.0 0.0 0.13356047700170356 0.0 24 0.0 0.0 0.0 0.13901192504258944 0.0 25 0.0 0.0 0.0 0.1526405451448041 0.0 26 0.0 0.0 0.0 0.16626916524701874 0.0 27 0.0 0.0 0.0 0.2657580919931857 0.0 28 0.0 0.0 0.0 0.39931856899488927 0.0 29 0.0 0.0 0.0 0.5056218057921635 0.0 30 0.0 0.0 0.0 0.6596252129471891 0.0 31 0.0 0.0 0.0 0.8136286201022147 0.0 32 0.0 0.0 0.0 0.9839863713798978 0.0 33 0.0 0.0 0.0 1.2388415672913118 0.0 34 0.0 0.0 0.0 1.5604770017035776 0.0 35 0.0 0.0 0.0 2.012947189097104 0.0 36 0.0 0.0 0.0 2.675298126064736 0.0 37 0.0 0.0 0.0 3.490289608177172 0.0 38 0.0 0.0 0.0 4.301192504258943 0.0 39 0.0 0.0 0.0 5.091652470187394 0.0 40 0.0 0.0 0.0 5.965247018739353 0.0 41 0.0 0.0 0.0 6.776149914821124 0.0 42 0.0 0.0 0.0 7.630664395229983 0.0 43 0.0 0.0 0.0 8.451107325383305 0.0 44 0.0 0.0 0.0 9.319250425894378 0.0 45 0.0 0.0 0.0 10.254173764906303 0.0 46 0.0 0.0 0.0 11.16320272572402 0.0 47 0.0 0.0 0.0 12.058603066439524 0.001362862010221465 48 0.0 0.0 0.0 12.904940374787053 0.001362862010221465 49 0.0 0.0 0.0 13.664054514480409 0.001362862010221465 50 0.0 0.0 0.0 14.378194207836456 0.001362862010221465 51 0.0 0.0 0.0 15.108688245315161 0.001362862010221465 52 0.0 0.0 0.0 15.81328790459966 0.001362862010221465 53 0.0 0.0 0.0 16.535604770017034 0.001362862010221465 54 0.0 0.0 0.0 17.271550255536628 0.001362862010221465 55 0.0 0.0 0.0 17.94344122657581 0.001362862010221465 56 0.0 0.0 0.0 18.57989778534923 0.001362862010221465 57 0.0 0.0 0.0 19.272231686541737 0.001362862010221465 58 0.0 0.0 0.0 19.890971039182283 0.001362862010221465 59 0.0 0.0 0.0 20.468824531516184 0.001362862010221465 60 0.0 0.0 0.0 21.038500851788758 0.001362862010221465 61 0.0 0.0 0.0 21.66541737649063 0.001362862010221465 62 0.0 0.0 0.0 22.245996592844975 0.001362862010221465 63 0.0 0.0 0.0 22.83884156729131 0.001362862010221465 64 0.0 0.0 0.0 23.38534923339012 0.001362862010221465 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGGAAT 15 0.002216587 70.0 19 TTTACAC 15 0.002216587 70.0 2 CAGCATC 15 0.002216587 70.0 4 ATAGCGA 15 0.002216587 70.0 39 TTACTCG 15 0.002216587 70.0 19 GGATAGC 15 0.002216587 70.0 37 AATATGC 15 0.002216587 70.0 67 AGCGGAG 15 0.002216587 70.0 56 TTTGACG 15 0.002216587 70.0 23 GTTGGAA 15 0.002216587 70.0 18 AGGTGAA 15 0.002216587 70.0 61 AAAACCT 25 2.3762583E-4 56.000004 68 ATTACTC 20 0.0069262474 52.500004 18 ATGCTCG 20 0.0069262474 52.500004 70 ATATGCT 20 0.0069262474 52.500004 68 TATGCTC 20 0.0069262474 52.500004 69 GCCATTA 20 0.0069262474 52.500004 27 GAGCGTT 20 0.0069262474 52.500004 9 AGTTGGA 20 0.0069262474 52.500004 17 TACTCGC 20 0.0069262474 52.500004 20 >>END_MODULE