FastQCFastQC Report
Wed 25 May 2016
SRR1780253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412698
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGG353.2356344E-431.3930024
TGCGAAC300.005728232729.34636113
CTGAATA300.00575205829.32145111
GTCTCAA300.005758879229.31434241
TATAAAT407.070879E-427.4688762
TATTAGA602.8903893E-421.97512
ATACCCA755.509268E-520.5100944
GGCGAAT550.00446405420.0113138
GAGATCT550.00446405420.0113139
GCTTAGA550.004508141419.9773641
GACGAAA909.965563E-619.56186523
GTATTAG809.048646E-519.2282121
TACCAAC809.048646E-519.2282127
TGTTAGA809.048646E-519.2282122
TGCGACA600.007348961718.35706916
ACCCAGA600.007374748618.34592824
GCCTAAG600.00737991518.34370234
TATGCAG600.00741616218.32812930
CTGAACC600.00745255518.3125844
CGACGAA2100.017.81527122
Produced by FastQC (version 0.11.5)