FastQCFastQC Report
Wed 25 May 2016
SRR1780253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412698
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT190144.60724306878153No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC55601.3472321164628855No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG48781.181978105055028No Hit
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG33810.8192431269354347Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC10360.25103102026178953No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT5370.13011936088859166Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTCCATATCTCGTATGCCGTCT4170.10104240873471643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATC250.00232938435.2663351
TTACTCG250.002351670535.19796419
AAACGTA250.002351670535.19796415
TTTTGCT250.002351670535.19796419
TCTTCTG4450.033.6160344
GTCTTCT4650.032.1701843
ATGCCGT24450.032.0308744
CTTTATA1400.031.4305632
CGTCTTC4950.030.22047442
CCGTCTT4950.030.22047441
TCTTTAT1500.029.388611
CCATAGT300.005738907529.3351923
TTGCTAC300.005742307829.33163621
CTATAGA300.005742307829.33163611
AAAACGT300.005742307829.33163614
CGTATTA407.0266076E-427.49841115
TATGCCG29750.026.25058443
TTTATAC2100.025.144453
TAGAGTT450.001397126224.44303127
GTAATCC450.001397126224.44303121
Produced by FastQC (version 0.11.5)