FastQCFastQC Report
Wed 25 May 2016
SRR1780252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48726
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC150.002212097970.0000132
CTTTGGA150.002212097970.0000120
GAACGAA150.002212097970.0000127
TCCCGGT207.852748E-570.043
CATTCCG200.006912329252.518
CCTCCGT200.006912329252.552
ATCTCTG350.001243967140.042
TTCTACA501.6865956E-435.0000042
ATCTAAC400.002396832535.010
TTATACC652.0631986E-532.3076932
TATACCC552.951859E-431.818183
GGGTGGG450.00426845831.1111091
TACCCAT802.4051787E-630.6255
CTTCCGA500.007143825828.00000218
TCGCCGT906.01805E-627.22222152
CTTCTAC1151.4492962E-624.3478281
AGATCTC750.001808063623.33333440
GTGGTCG901.892235E-423.33333248
TATCTCT952.7288002E-422.10526541
GGTGGTC952.7288002E-422.10526547
Produced by FastQC (version 0.11.5)