##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780252_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48726 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.866888314247014 34.0 23.0 34.0 12.0 34.0 2 27.901161597504412 34.0 23.0 34.0 12.0 34.0 3 27.434388211632392 34.0 21.0 34.0 11.0 34.0 4 27.50205229241062 34.0 23.0 34.0 11.0 34.0 5 27.43196650658786 34.0 23.0 34.0 11.0 34.0 6 29.7593892377786 36.0 23.0 38.0 11.0 38.0 7 30.145363871444403 36.0 24.0 38.0 12.0 38.0 8 30.223843533226614 36.0 25.0 38.0 11.0 38.0 9 30.429154865985307 37.0 24.0 38.0 12.0 38.0 10-11 30.017967820055002 36.0 23.5 38.0 11.0 38.0 12-13 30.101085662685218 36.0 23.0 38.0 11.0 38.0 14-15 29.860084964905802 36.0 22.0 38.0 11.0 38.0 16-17 29.899571070886182 36.0 22.5 38.0 11.0 38.0 18-19 29.968569141731315 36.5 22.5 38.0 11.0 38.0 20-21 29.94541928333949 36.0 22.5 38.0 11.0 38.0 22-23 29.942730780281572 36.5 22.5 38.0 11.0 38.0 24-25 29.89921191971432 36.5 22.5 38.0 11.0 38.0 26-27 29.789137216270575 36.0 22.0 38.0 11.0 38.0 28-29 29.81308746870254 36.5 22.0 38.0 11.0 38.0 30-31 29.836904322127815 37.0 22.0 38.0 11.0 38.0 32-33 29.904034806879285 37.0 22.0 38.0 11.0 38.0 34-35 29.872121659894102 36.5 22.0 38.0 11.0 38.0 36-37 29.823410499527974 36.0 21.5 38.0 11.0 38.0 38-39 29.731293354677177 36.5 21.5 38.0 11.0 38.0 40-41 29.798013380946518 36.5 22.0 38.0 11.0 38.0 42-43 29.922782498050324 37.0 22.5 38.0 11.0 38.0 44-45 30.079454500677258 37.0 23.0 38.0 11.0 38.0 46-47 29.919303862414317 37.0 22.0 38.0 11.0 38.0 48-49 29.778229282108114 37.0 21.5 38.0 11.0 38.0 50-51 29.680581209210686 36.5 21.0 38.0 11.0 38.0 52-53 29.88024873784017 36.5 22.5 38.0 11.0 38.0 54-55 29.801163649796823 37.0 22.0 38.0 11.0 38.0 56-57 29.72070352583836 36.5 22.0 38.0 11.0 38.0 58-59 29.74132906456512 36.5 22.0 38.0 11.0 38.0 60-61 29.783924393547593 37.0 22.5 38.0 11.0 38.0 62-63 29.62485120880023 36.0 22.0 38.0 11.0 38.0 64-65 29.54178467348028 36.0 21.5 38.0 11.0 38.0 66-67 29.471206337478964 36.0 22.0 38.0 11.0 38.0 68-69 29.357191232606823 36.0 21.0 38.0 11.0 38.0 70-71 29.272010836103927 36.0 21.0 38.0 11.0 38.0 72-73 29.166800065673357 36.0 20.0 38.0 11.0 38.0 74-75 29.096734802774698 36.0 20.0 38.0 11.0 38.0 76 27.495115544062717 32.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5953094833268544 1101 2 -0.42750746835920594 1101 3 -0.2633569080833347 1101 4 -0.3452426731071405 1101 5 -0.5401248225366295 1101 6 0.5819294261907437 1101 7 -0.27411485364207877 1101 8 -0.500819437045898 1101 9 0.1732401565029491 1101 10-11 -0.950064389897598 1101 12-13 -0.8521434846596421 1101 14-15 -1.2606608347183226 1101 16-17 -0.6941977136614526 1101 18-19 -0.9544288430795476 1101 20-21 -0.5715980906230875 1101 22-23 -1.2292219979554275 1101 24-25 -1.226707211561557 1101 26-27 -1.1627636327651345 1101 28-29 -1.3495245229828825 1101 30-31 -0.5810441532713462 1101 32-33 -0.831326240957555 1101 34-35 -0.23284132689540016 1101 36-37 -0.6405362219678459 1101 38-39 -0.9985449387507686 1101 40-41 -0.11621387843152675 1101 42-43 -0.6169016836860024 1101 44-45 -0.08684023728373091 1101 46-47 -0.43801128904233977 1101 48-49 -0.34178449329115423 1101 50-51 -0.33331729776347174 1101 52-53 0.09599828954540257 1101 54-55 0.08535315367097596 1101 56-57 -0.6526479052919463 1101 58-59 -0.18261332090990479 1101 60-61 -0.7768424594389813 1101 62-63 -0.6198375456340166 1101 64-65 -0.3246407793925492 1101 66-67 -0.212703530921992 1101 68-69 -0.8853032951347437 1101 70-71 -0.8295870943679553 1101 72-73 -0.16700271178148896 1101 74-75 0.17414050065046993 1101 76 -0.013745526949609399 1102 1 -0.312400937599719 1102 2 0.17803975802760164 1102 3 -0.08264726290592606 1102 4 0.2785954078523787 1102 5 0.2993554373334355 1102 6 -0.46994463677777176 1102 7 -1.218042889624087 1102 8 0.15080475086015 1102 9 0.43430962177031773 1102 10-11 0.0692759399374836 1102 12-13 0.27556765976814646 1102 14-15 1.2710233232027157 1102 16-17 0.16694671412465567 1102 18-19 0.7115381734122082 1102 20-21 -0.12339719107286484 1102 22-23 0.4357954932989472 1102 24-25 0.4195196749951613 1102 26-27 0.5236931388490582 1102 28-29 -0.38403226910982013 1102 30-31 -0.05875529769913257 1102 32-33 0.38743937623384994 1102 34-35 0.05758845821204872 1102 36-37 0.23919891046594088 1102 38-39 0.36592282736617676 1102 40-41 -0.6278330688363276 1102 42-43 0.20843564764832934 1102 44-45 0.7145340755598468 1102 46-47 -0.26999529703834213 1102 48-49 -0.3932837436659682 1102 50-51 -0.15683054114178674 1102 52-53 0.27535860938548495 1102 54-55 -0.08098867541448129 1102 56-57 1.123514013748533 1102 58-59 -0.32941492011030604 1102 60-61 0.1620131127749147 1102 62-63 -0.19027732574395984 1102 64-65 0.19952213914817918 1102 66-67 0.06803110176166527 1102 68-69 -0.338676608478071 1102 70-71 0.5076942649523843 1102 72-73 0.2618578579336521 1102 74-75 -0.2648150215884115 1102 76 -0.5039504245008359 1103 1 -0.4502866000316601 1103 2 0.2607076346156241 1103 3 -0.4120029797843294 1103 4 -0.2866042291712141 1103 5 -1.3529585372582176 1103 6 0.1079591744744981 1103 7 -0.7658196591340705 1103 8 0.5384635530380102 1103 9 -1.2036667847632607 1103 10-11 -1.2651387606069804 1103 12-13 -0.24335248542241672 1103 14-15 -1.358105533421604 1103 16-17 0.26785415514098787 1103 18-19 -0.1377812458255434 1103 20-21 0.7177325729924711 1103 22-23 -0.2860965212201094 1103 24-25 -1.0825901253678119 1103 26-27 -0.9714974237643688 1103 28-29 -0.5206248280934851 1103 30-31 -0.4497035735612016 1103 32-33 -0.14091815552658815 1103 34-35 -0.7313634474140862 1103 36-37 -0.1331468245612868 1103 38-39 -1.3229156481329198 1103 40-41 -0.990355754861735 1103 42-43 -0.5652712489033931 1103 44-45 -0.5019813509374274 1103 46-47 -0.7490334095610258 1103 48-49 0.2671972000726903 1103 50-51 -0.08541493315630433 1103 52-53 -0.40390636339128605 1103 54-55 -0.10372007287364582 1103 56-57 0.20976628235107597 1103 58-59 -0.6074321614896157 1103 60-61 0.2972898822848933 1103 62-63 -0.2706765997911482 1103 64-65 -0.06143521112405281 1103 66-67 -0.6376177185650143 1103 68-69 -0.4805550113818846 1103 70-71 -0.36701081671730407 1103 72-73 0.12490232254345557 1103 74-75 -0.11070885098950001 1103 76 -0.28453118675128763 1104 1 0.6118777393883157 1104 2 -0.38743056803385656 1104 3 1.1873973066460337 1104 4 0.5294985277210174 1104 5 0.5701975672888651 1104 6 -1.0028088169325926 1104 7 -0.19425682347348783 1104 8 -0.15935241545465217 1104 9 0.7035566309188042 1104 10-11 0.8485159117882901 1104 12-13 0.23887988120844383 1104 14-15 0.021504205210693073 1104 16-17 0.6608008074861331 1104 18-19 0.6535273828890986 1104 20-21 0.4710668014206867 1104 22-23 -0.17494811448899839 1104 24-25 -0.16710945941722244 1104 26-27 -0.22901622075685424 1104 28-29 0.37444767459179573 1104 30-31 0.7854154740578458 1104 32-33 0.5811996389596281 1104 34-35 0.8274219088336743 1104 36-37 0.3444534260652823 1104 38-39 0.2008043661885992 1104 40-41 0.07507568087045158 1104 42-43 0.5780110640217764 1104 44-45 0.7715596444080788 1104 46-47 0.3790546677751685 1104 48-49 0.6662752523462316 1104 50-51 0.21703762681505268 1104 52-53 0.4337681801103237 1104 54-55 0.2961635647919465 1104 56-57 0.6188693972812587 1104 58-59 -0.12146277373451042 1104 60-61 -0.036602885348472114 1104 62-63 0.2169710908640603 1104 64-65 0.5976455264341247 1104 66-67 0.7759832481374609 1104 68-69 0.8186304522772652 1104 70-71 1.1093363010771782 1104 72-73 0.36279616429068184 1104 74-75 0.4386332542736575 1104 76 0.45105778567979016 1105 1 1.14320858158435 1105 2 0.8906186798343043 1105 3 0.01210747240247656 1105 4 0.614340915380307 1105 5 1.1147026932829256 1105 6 0.1743972767724955 1105 7 0.3612188879378344 1105 8 -0.21616950848904892 1105 9 0.8436831040432651 1105 10-11 0.7800335892818211 1105 12-13 0.15295959760204525 1105 14-15 1.3186920944508955 1105 16-17 0.27156060436263374 1105 18-19 0.7822521122174493 1105 20-21 0.06266400368187064 1105 22-23 0.3383938420119108 1105 24-25 0.5945318168115783 1105 26-27 0.7875833768286604 1105 28-29 0.34311683239576496 1105 30-31 0.5652612151711907 1105 32-33 0.47388910911388393 1105 34-35 0.6931639803101497 1105 36-37 0.8592086239190699 1105 38-39 0.5888708603919142 1105 40-41 0.4761008796823738 1105 42-43 0.006031567998014964 1105 44-45 0.33901197745397127 1105 46-47 0.6675714829519457 1105 48-49 0.3786686604136804 1105 50-51 0.2274093811505935 1105 52-53 0.21775783230923196 1105 54-55 0.12561647271567722 1105 56-57 -0.11223149377939023 1105 58-59 0.25084248639770834 1105 60-61 0.9510611943679201 1105 62-63 0.10867847804428621 1105 64-65 0.07074603424288384 1105 66-67 0.3243448462978513 1105 68-69 -0.3012555302847737 1105 70-71 0.4959895539276147 1105 72-73 0.6491332343299625 1105 74-75 0.7837036768088694 1105 76 1.2384487984229153 1106 1 0.3738803821720502 1106 2 0.9073376989851809 1106 3 0.9239019804580053 1106 4 1.2595430635209475 1106 5 1.1451009099208846 1106 6 0.738638841267381 1106 7 0.3539739796391217 1106 8 0.45856957348480876 1106 9 1.0117345783567728 1106 10-11 0.15096782780649676 1106 12-13 0.8883684536158256 1106 14-15 1.131008438558709 1106 16-17 0.77277653302815 1106 18-19 0.5682486670862303 1106 20-21 1.1596343147569534 1106 22-23 1.0245575870722021 1106 24-25 0.8130696625912925 1106 26-27 0.9477566466634961 1106 28-29 1.5548910749735363 1106 30-31 0.7485877933452727 1106 32-33 1.1935668880175214 1106 34-35 0.46049096379378796 1106 36-37 0.7973482530608287 1106 38-39 0.634392189807258 1106 40-41 0.4118593151874883 1106 42-43 0.7085596863484049 1106 44-45 0.6307335297895662 1106 46-47 0.7607166851000855 1106 48-49 1.1443744056766292 1106 50-51 0.847659659800911 1106 52-53 0.668462057661344 1106 54-55 0.31595997256368946 1106 56-57 0.9799764300224112 1106 58-59 1.6439167593736919 1106 60-61 1.5691329341591853 1106 62-63 0.654351798542816 1106 64-65 0.799720601068298 1106 66-67 0.6780534287276794 1106 68-69 0.6622412779024778 1106 70-71 1.0161537042974587 1106 72-73 0.9667945981966177 1106 74-75 0.5069785780146638 1106 76 0.5884335993145946 1107 1 -0.12791817897903002 1107 2 -0.6409736519340825 1107 3 0.7239619324963726 1107 4 -0.675858615136125 1107 5 -0.4187480109424264 1107 6 0.9409270107318086 1107 7 0.09273297244487821 1107 8 0.2500863600555512 1107 9 -0.01909076137144794 1107 10-11 0.5621638709719647 1107 12-13 0.8498492689635455 1107 14-15 0.5424792619000343 1107 16-17 0.4721670206380608 1107 18-19 0.767189514408372 1107 20-21 0.6391507016474414 1107 22-23 -0.405248568003735 1107 24-25 -0.11091614875962463 1107 26-27 0.4078885411479085 1107 28-29 1.020493592959145 1107 30-31 0.24474087088324126 1107 32-33 0.4343742421342327 1107 34-35 0.3903470789017014 1107 36-37 1.0379058070176619 1107 38-39 0.1394381530366715 1107 40-41 1.1331822568341714 1107 42-43 0.29583986220771763 1107 44-45 0.7114689414602324 1107 46-47 0.16434378342142963 1107 48-49 0.22388579656391983 1107 50-51 -0.35807575186975527 1107 52-53 -0.2308434978942131 1107 54-55 -0.1519656869087349 1107 56-57 -0.20744862992962965 1107 58-59 0.10090117184372005 1107 60-61 -0.1619571937384947 1107 62-63 0.09317573939013712 1107 64-65 0.09006332688764118 1107 66-67 0.00876386038235566 1107 68-69 0.6088090237058381 1107 70-71 0.058245031235909295 1107 72-73 -0.14556551371385495 1107 74-75 0.8309943653847682 1107 76 1.029430801552806 1108 1 -0.3431959567568086 1108 2 -0.9743069852674182 1108 3 -0.5677047341702917 1108 4 -0.5647475040250143 1108 5 0.2687519890575736 1108 6 -0.9896285448237485 1108 7 -1.0669892497773432 1108 8 -1.7568580843888917 1108 9 -1.7204796502603337 1108 10-11 -0.7885305734724781 1108 12-13 -1.330706286592008 1108 14-15 -1.190159626988855 1108 16-17 -0.8889440904730463 1108 18-19 -1.5314215967027351 1108 20-21 -1.2358492983525586 1108 22-23 -1.2976096791148493 1108 24-25 -0.5900828154262925 1108 26-27 -0.6685003477409808 1108 28-29 -1.7954786292630764 1108 30-31 -1.3872035735612016 1108 32-33 -1.7635424245324351 1108 34-35 -0.8596529210982986 1108 36-37 -1.0454275263156738 1108 38-39 -0.4057007358522142 1108 40-41 -1.8911232987213822 1108 42-43 -1.2423545822367288 1108 44-45 -1.4204755029842104 1108 46-47 -0.9085728832452382 1108 48-49 -1.508753092324973 1108 50-51 -1.5351590852030874 1108 52-53 -2.3037601645608774 1108 54-55 -1.0026601313531778 1108 56-57 -0.8463375188185154 1108 58-59 -1.1977099392673942 1108 60-61 -0.8147349715162733 1108 62-63 0.1730368505012514 1108 64-65 -0.06271445089013739 1108 66-67 -0.8731805840620872 1108 68-69 -1.1620243096274976 1108 70-71 -0.7125883020974229 1108 72-73 -0.4997321803805228 1108 74-75 -1.1169223012818996 1108 76 -1.6997358651138619 1109 1 1.7611795653818731 1109 2 0.9204820707058481 1109 3 0.6979594262307103 1109 4 0.8102002821069831 1109 5 0.6385828161252434 1109 6 0.5224328586850255 1109 7 0.5791051528960054 1109 8 0.5083883650680825 1109 9 0.5059196814021547 1109 10-11 0.9994289085659496 1109 12-13 0.6542039055550255 1109 14-15 1.1926463462776447 1109 16-17 0.5197338543891092 1109 18-19 0.2954894308661835 1109 20-21 0.14489422712781064 1109 22-23 0.7002756592310178 1109 24-25 1.7722031077148905 1109 26-27 0.4439161100702158 1109 28-29 0.39055938243282995 1109 30-31 0.5159919151606047 1109 32-33 0.8055104159019848 1109 34-35 0.8057606127362895 1109 36-37 0.9020975363409711 1109 38-39 1.0285881112655773 1109 40-41 0.7634036436345077 1109 42-43 1.178619728540216 1109 44-45 0.5882442129723415 1109 46-47 0.9821695979577676 1109 48-49 1.70422727526066 1109 50-51 0.6232316833850504 1109 52-53 0.07823649375156805 1109 54-55 0.08331000231432384 1109 56-57 0.6054429740803968 1109 58-59 0.6223610715930938 1109 60-61 0.7686244687510637 1109 62-63 0.8759399415622333 1109 64-65 1.1871550144398562 1109 66-67 0.6589048378259648 1109 68-69 0.7647457404978155 1109 70-71 1.081767378771417 1109 72-73 0.7654098413404462 1109 74-75 1.1679565625443367 1109 76 0.9523109185118699 1110 1 0.9108142153871661 1110 2 0.9187876939188122 1110 3 0.6599947963461403 1110 4 1.4453268308732632 1110 5 0.5865336791984177 1110 6 1.1462282626879912 1110 7 1.2274160710364264 1110 8 1.3472107732010912 1110 9 1.6535422589728412 1110 10-11 0.6004561244930926 1110 12-13 0.6494384708414778 1110 14-15 0.3820723761410356 1110 16-17 1.507476097320378 1110 18-19 1.201266196724493 1110 20-21 0.8549175248086307 1110 22-23 0.6412107434203627 1110 24-25 0.11917970413552226 1110 26-27 0.8051890106314765 1110 28-29 1.0438210577478806 1110 30-31 1.2448386799599263 1110 32-33 0.8682553062969873 1110 34-35 0.34105130425381347 1110 36-37 1.0810396098345656 1110 38-39 1.761406071659522 1110 40-41 0.8634248233443422 1110 42-43 0.8546231172937908 1110 44-45 1.2509838084398872 1110 46-47 0.7309517646420858 1110 48-49 0.5195137308362163 1110 50-51 0.6006488177703098 1110 52-53 0.6966310717458519 1110 54-55 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-0.13697951408325437 2116 38-39 0.7228351968694966 2116 40-41 0.8318315942449779 2116 42-43 0.2736048978855976 2116 44-45 0.4678399914092637 2116 46-47 1.6897642727180653 2116 48-49 0.004944665065444553 2116 50-51 0.20321374863993213 2116 52-53 -0.831537942338656 2116 54-55 0.22685694306068527 2116 56-57 -0.4701588745779439 2116 58-59 0.06286600364800066 2116 60-61 0.06603082868760168 2116 62-63 0.29523358852367565 2116 64-65 0.012049820261751165 2116 66-67 0.7126056035016219 2116 68-69 0.08577767814008652 2116 70-71 0.9898518100330556 2116 72-73 -0.17468121198092845 2116 74-75 0.25514701574154586 2116 76 -0.5860763340231365 2117 1 -0.6349275247734241 2117 2 -0.6690759364616028 2117 3 -0.43997887747247333 2117 4 -0.5603991135784945 2117 5 -1.0787947399143896 2117 6 -0.33639645759633297 2117 7 -1.0538791770878326 2117 8 -1.5569619265488086 2117 9 -1.939586607685051 2117 10-11 -0.9860253813644846 2117 12-13 -1.6172457466127774 2117 14-15 -1.0382580174353748 2117 16-17 -1.018422283619465 2117 18-19 -1.3863670795687817 2117 20-21 -2.0050600979371893 2117 22-23 -1.626756875376529 2117 24-25 -0.6837030127263937 2117 26-27 -1.7210055398489743 2117 28-29 -1.2852891173212306 2117 30-31 -0.34631572309391245 2117 32-33 -0.263704677907306 2117 34-35 -0.20077441642539995 2117 36-37 -1.2045007350384118 2117 38-39 -0.4688822000266697 2117 40-41 -1.176656787817958 2117 42-43 -1.1674908750716178 2117 44-45 -0.35343240848784774 2117 46-47 -1.1185612010022474 2117 48-49 -1.2189685648068007 2117 50-51 -1.2849968318282166 2117 52-53 0.004910655792187413 2117 54-55 -0.3088971510831868 2117 56-57 -1.5122578199607801 2117 58-59 -1.6874555259755972 2117 60-61 -1.4058889175183502 2117 62-63 -0.9701355274842136 2117 64-65 -1.328128521502805 2117 66-67 -1.7759908125148414 2117 68-69 -1.1410936242692422 2117 70-71 -1.2996534630527705 2117 72-73 -2.134597185572627 2117 74-75 -2.0800583345113886 2117 76 -0.12393108837450484 2118 1 0.1628981475515836 2118 2 0.5955626292450553 2118 3 0.030934721612020155 2118 4 -0.28002528180279285 2118 5 -1.0284418884934468 2118 6 -0.4926047352999383 2118 7 -0.5309915173510404 2118 8 -0.645746973277781 2118 9 0.04029132479635322 2118 10-11 -1.7535249045382386 2118 12-13 -0.6352697786555019 2118 14-15 -0.20638695125189344 2118 16-17 -0.6419202809492397 2118 18-19 -0.6696018688115828 2118 20-21 -0.9251917019806761 2118 22-23 -0.38753314850260523 2118 24-25 -0.37629313288661237 2118 26-27 0.602616432304373 2118 28-29 -0.9469808968367737 2118 30-31 -0.25710153274487624 2118 32-33 -0.9885282521968364 2118 34-35 0.2678980993098641 2118 36-37 -0.17765541747213476 2118 38-39 -0.56110039571616 2118 40-41 -0.6068971082451959 2118 42-43 -0.6430773713523728 2118 44-45 -0.6400049814422637 2118 46-47 -1.1209453322248315 2118 48-49 -1.056513863300026 2118 50-51 -1.0397650942733812 2118 52-53 -0.6070481464202899 2118 54-55 -1.3551969794257417 2118 56-57 -0.350518357820782 2118 58-59 -0.23880971251002236 2118 60-61 -0.4770790758246619 2118 62-63 -1.2167023785236957 2118 64-65 -0.5639190994162142 2118 66-67 -0.4995269559441766 2118 68-69 -1.3633423368383824 2118 70-71 -0.25630995742622176 2118 72-73 -0.49264601258006735 2118 74-75 -0.9083055212365458 2118 76 -1.065949017041298 2119 1 0.550653249592397 2119 2 -0.1493353299386193 2119 3 -0.30579997226553246 2119 4 -0.9636987511905488 2119 5 0.48176219313920754 2119 6 0.7727013871490414 2119 7 0.6730901153020206 2119 8 1.7726203736609953 2119 9 1.8566178554085973 2119 10-11 1.3842301975025748 2119 12-13 1.2830975682832744 2119 14-15 1.069123252829737 2119 16-17 0.8121613517038213 2119 18-19 0.7130511924129088 2119 20-21 0.023787889856059508 2119 22-23 0.43899746374229665 2119 24-25 0.24615584670522495 2119 26-27 0.260779697610495 2119 28-29 0.6040395113264942 2119 30-31 0.05922499786736779 2119 32-33 0.6951452171909196 2119 34-35 -0.06373455375135961 2119 36-37 0.011120092731946585 2119 38-39 -0.4539575385733059 2119 40-41 -0.5864889449798873 2119 42-43 0.5814124245659968 2119 44-45 0.38040318182304134 2119 46-47 0.2515036473670058 2119 48-49 0.4485881775162994 2119 50-51 0.3530920485837612 2119 52-53 1.2756049148042017 2119 54-55 0.3948030205742583 2119 56-57 0.8892775605465637 2119 58-59 0.057108654836913075 2119 60-61 0.7014923527467616 2119 62-63 -0.20649829689103782 2119 64-65 -0.3292252218651903 2119 66-67 0.10251386038235566 2119 68-69 -0.2459953980628704 2119 70-71 1.0192002466554086 2119 72-73 1.175721334358709 2119 74-75 0.6172046828450881 2119 76 0.6891530237750274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 14.0 13 246.0 14 896.0 15 1605.0 16 2086.0 17 2391.0 18 2346.0 19 2068.0 20 1773.0 21 1192.0 22 885.0 23 623.0 24 589.0 25 501.0 26 515.0 27 555.0 28 609.0 29 735.0 30 790.0 31 1041.0 32 1236.0 33 1571.0 34 2091.0 35 2967.0 36 5216.0 37 14185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.309239420432625 18.967286458974677 11.316340352173377 37.40713376841933 2 16.964249066206953 18.359807905430365 34.76378114353733 29.912161884825352 3 20.4367278249805 19.449575175471 27.681320034478514 32.432376965069984 4 14.58974674711653 18.58761236300948 29.694618889299345 37.12802200057464 5 15.386036202438124 28.910643188441487 33.7827853712597 21.92053523786069 6 27.61769896974921 31.26872716824693 16.804170258178385 24.309403603825473 7 25.620818454213357 28.013791404999385 17.98629068669704 28.379099454090216 8 21.637318885194762 27.859869474202686 18.072486967943195 32.43032467265936 9 21.479292369576815 17.72975413536921 18.56503714649263 42.22591634856134 10-11 24.898411525674177 21.63424044657883 23.733735582645814 29.733612445101176 12-13 22.678857283585767 18.907769979066618 24.29914214177236 34.11423059557526 14-15 23.910232729959365 19.88055658170176 21.78918852358084 34.42002216475804 16-17 25.523334564708776 20.887206009112177 21.78713623117022 31.802323195008825 18-19 24.491031482165578 19.74100069777942 22.26018963181874 33.50777818823626 20-21 23.93896482370808 20.477773673192956 22.932315396297664 32.6509461068013 22-23 26.564872963099784 20.003694126339123 20.972376144153017 32.45905676640808 24-25 25.06259491852399 21.991339326027173 20.04884455937282 32.89722119607602 26-27 23.648565447604973 22.522883060378444 22.623445388498954 31.205106103517625 28-29 24.809391386440367 20.986957547895866 24.004884506059458 30.198766559604312 30-31 24.535155768993967 20.085785822764027 24.578253909617043 30.800804498624963 32-33 23.39818577350901 21.8220252021508 24.50539752903994 30.27439149530025 34-35 23.103681812584657 21.818946763534868 24.218076591552766 30.85929483232771 36-37 23.889709805853137 19.705085580593522 25.014366046874358 31.39083856667898 38-39 22.643968312605182 19.226901448918444 28.12769363378894 30.001436604687438 40-41 22.08984936173706 19.38184952592045 28.041497352542788 30.486803759799695 42-43 20.087838115174648 19.281287197799944 27.667980133809465 32.962894553215946 44-45 19.274104174362762 18.973443336206543 30.394450601321676 31.358001888109015 46-47 19.543980626359645 20.862578500184707 29.622788654927557 29.970652218528095 48-49 19.569634281492426 21.409514427615647 28.519681484217873 30.501169806674056 50-51 19.729713089520995 23.0985510815581 26.005623281205104 31.166112547715798 52-53 18.313631326191356 22.8902434018799 26.829618684070105 31.96650658785864 54-55 18.69433156836186 21.967737963305012 26.876821409514427 32.4611090588187 56-57 19.65583056273858 22.316627673110865 25.750112876082582 32.27742888806797 58-59 19.48241185404096 22.249928169765628 25.212412264499445 33.055247711693966 60-61 18.970364897590606 20.737388663136723 26.04769527562287 34.244551163649795 62-63 19.507039362968438 21.403357550383777 25.4073800435086 33.68222304313919 64-65 20.374132906456513 21.17452694659935 24.191396790214668 34.259943356729465 66-67 19.667118170997004 23.333538562574397 22.254032754586873 34.74531051184172 68-69 20.185322004679225 23.712186512334277 21.981077863974058 34.121413619012436 70-71 21.037023355087634 22.839962237819645 22.676804991175143 33.44620941591758 72-73 20.830767967820055 21.6137175224726 25.221647580347252 32.33386692936009 74-75 21.150925583877193 21.3961745269466 25.525386857119404 31.927513032056808 76 20.8348725526413 20.145302302672086 26.057956737675987 32.96186840701063 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.5 14 2.5 15 2.5 16 2.0 17 3.0 18 2.5 19 4.0 20 10.5 21 15.0 22 19.5 23 27.5 24 41.0 25 51.0 26 72.0 27 98.5 28 125.5 29 147.0 30 192.5 31 295.5 32 405.0 33 457.0 34 521.5 35 697.5 36 877.5 37 946.0 38 1037.5 39 1265.0 40 1503.0 41 1676.5 42 1748.0 43 1813.5 44 1924.0 45 2035.0 46 2101.0 47 2050.5 48 1969.0 49 1876.0 50 1814.0 51 1717.0 52 1504.0 53 1313.5 54 1239.0 55 1215.5 56 1198.0 57 1232.0 58 1260.0 59 1331.5 60 1484.5 61 1630.5 62 1695.0 63 1722.0 64 1833.0 65 1903.0 66 1878.0 67 1867.0 68 1806.0 69 1667.0 70 1427.0 71 1265.0 72 1128.5 73 856.0 74 595.5 75 471.0 76 364.5 77 187.5 78 81.5 79 46.0 80 27.0 81 7.5 82 8.5 83 10.0 84 8.5 85 6.0 86 4.0 87 3.0 88 3.0 89 1.5 90 1.5 91 2.0 92 1.0 93 2.5 94 4.5 95 5.0 96 5.0 97 4.0 98 2.5 99 7.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0010261462053113327 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48726.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.40105898288388 #Duplication Level Percentage of deduplicated Percentage of total 1 86.43880112210248 72.09087550794237 2 9.609232737831586 16.028403726963017 3 2.655150351887396 6.643270533185568 4 0.7554505635119838 2.520215080244633 5 0.28544711846055415 1.190329598161146 6 0.13534130616664206 0.6772564955054796 7 0.054136522466656824 0.31605303123589046 8 0.029529012254540087 0.19702007141977587 9 0.009843004084846695 0.07388252678241596 >10 0.027068261233328412 0.2626934285597012 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008209169642490662 0.0 2 0.0 0.0 0.0 0.014366046874358659 0.0 3 0.0 0.0 0.0 0.014366046874358659 0.0 4 0.0 0.0 0.0 0.028732093748717318 0.0 5 0.0 0.0 0.0 0.03283667856996265 0.0 6 0.0 0.0 0.0 0.03283667856996265 0.0 7 0.0 0.0 0.0 0.03694126339120798 0.0 8 0.0 0.0 0.0 0.03694126339120798 0.0 9 0.0 0.0 0.0 0.053359602676189305 0.0 10 0.0 0.0 0.0 0.057464187497434635 0.0 11 0.0 0.0 0.0 0.057464187497434635 0.0 12 0.0 0.0 0.0 0.0656733571399253 0.0 13 0.0 0.0 0.0 0.06772564955054797 0.0 14 0.0 0.0 0.0 0.06772564955054797 0.0 15 0.0 0.0 0.0 0.0718302343717933 0.0 16 0.0 0.0 0.0 0.07798711160366129 0.0 17 0.0 0.0 0.0 0.08003940401428396 0.0 18 0.0 0.0 0.0 0.08003940401428396 0.0 19 0.0 0.0 0.0 0.08003940401428396 0.0 20 0.0 0.0 0.0 0.08824857365677462 0.0 21 0.0 0.0 0.0 0.09030086606739729 0.0 22 0.0 0.0 0.0 0.10466691294175594 0.0 23 0.0 0.0 0.0 0.11492837499486927 0.0 24 0.0 0.0 0.0 0.13339900669047325 0.0 25 0.0 0.0 0.0 0.13545129910109593 0.0 26 0.0 0.0 0.0 0.16213110043919057 0.0 27 0.0 0.0 0.0 0.21138611829413456 0.0 28 0.0 0.0 0.0 0.34888970980585315 0.0 29 0.0 0.0 0.0 0.5130731026556664 0.0 30 0.0 0.0 0.0 0.6957271272010837 0.0 31 0.0 0.0 0.0 0.8681196896933875 0.0 32 0.0 0.0 0.0 1.0343553749538235 0.0 33 0.0 0.0 0.0 1.2683167097648074 0.0 34 0.0 0.0 0.0 1.6951935311743218 0.0 35 0.0 0.0 0.0 2.2616262365061774 0.0 36 0.0 0.0 0.0 2.994294627098469 0.0 37 0.0 0.0 0.0 3.9609243525017446 0.0 38 0.0 0.0 0.0 4.796207363625169 0.0 39 0.0 0.0 0.0 5.680745392603538 0.0 40 0.0 0.0 0.0 6.530394450601322 0.0 41 0.0 0.0 0.0 7.390304970652219 0.0 42 0.0 0.0 0.0 8.289209046504945 0.0 43 0.0 0.0 0.0 9.264047941550713 0.0 44 0.0 0.0 0.0 10.14037680088659 0.0 45 0.0 0.0 0.0 11.022862537454337 0.0 46 0.0 0.0 0.0 11.837622624471535 0.0 47 0.0 0.0 0.0 12.60312769363379 0.0 48 0.0 0.0 0.0 13.307063990477364 0.0 49 0.0 0.0 0.0 13.935065468127899 0.0 50 0.0 0.0 0.0 14.476870664532283 0.0 51 0.0 0.0 0.0 15.055617124327874 0.0 52 0.0 0.0 0.0 15.755448836350203 0.0 53 0.0 0.0 0.0 16.45528054837253 0.0 54 0.0 0.0 0.0 17.06891597914871 0.0 55 0.0 0.0 0.0 17.746172474654188 0.0 56 0.0 0.0 0.0 18.287977671058574 0.0 57 0.0 0.0 0.0 18.883142470139145 0.0 58 0.0 0.0 0.0 19.394163280384188 0.0 59 0.0 0.0 0.0 19.87029511964865 0.0 60 0.0 0.0 0.0 20.446989287033617 0.0 61 0.0 0.0 0.0 20.953905512457414 0.0 62 0.0 0.0 0.0 21.54291343430612 0.0 63 0.0 0.0 0.0 22.01288839633871 0.0 64 0.0 0.0 0.0 22.616262365061775 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACGGC 15 0.0022120979 70.00001 32 CTTTGGA 15 0.0022120979 70.00001 20 GAACGAA 15 0.0022120979 70.00001 27 TCCCGGT 20 7.852748E-5 70.0 43 CATTCCG 20 0.0069123292 52.5 18 CCTCCGT 20 0.0069123292 52.5 52 ATCTCTG 35 0.0012439671 40.0 42 TTCTACA 50 1.6865956E-4 35.000004 2 ATCTAAC 40 0.0023968325 35.0 10 TTATACC 65 2.0631986E-5 32.307693 2 TATACCC 55 2.951859E-4 31.81818 3 GGGTGGG 45 0.004268458 31.111109 1 TACCCAT 80 2.4051787E-6 30.625 5 CTTCCGA 50 0.0071438258 28.000002 18 TCGCCGT 90 6.01805E-6 27.222221 52 CTTCTAC 115 1.4492962E-6 24.347828 1 AGATCTC 75 0.0018080636 23.333334 40 GTGGTCG 90 1.892235E-4 23.333332 48 TATCTCT 95 2.7288002E-4 22.105265 41 GGTGGTC 95 2.7288002E-4 22.105265 47 >>END_MODULE