FastQCFastQC Report
Wed 25 May 2016
SRR1780252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48726
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT732415.030989615400403No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC20074.11895086811969No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG19323.9650289373229897No Hit
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG17003.488897098058531Illumina PCR Primer Index 9 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC5331.0938718548618807No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT2990.613635430776177Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT1790.36736034150145713No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1300.2667980133809465No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1250.25653655132783315No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1240.2544842589172105No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1060.21754299552600256No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGC810.16623568526043592No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT790.16213110043919057Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC690.1416081763329639No Hit
CTTATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC640.1313467142798506No Hit
ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT600.12313754463735993Illumina PCR Primer Index 9 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT550.1128760825842466No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC540.11082379017362395No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG510.10466691294175594No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACC150.002212097970.0000132
GGCAAGA150.002212097970.0000112
GTGGCAA150.002212097970.0000110
TACATTT150.002212097970.0000126
ATCGTAT150.002212097970.0000139
TGGCAAG150.002212097970.0000111
ACGATAG150.002212097970.0000149
TCTACCC150.002212097970.0000133
TACGATA150.002212097970.0000148
CTACGAT150.002212097970.0000147
GAAACTT150.002212097970.0000163
GAAAACT150.002212097970.0000142
CTACATT150.002212097970.0000125
TGGGCTA150.002212097970.0000121
GCTACAT150.002212097970.0000124
CATTTTC150.002212097970.0000128
TTTCTAC150.002212097970.0000131
ACCCCAG150.002212097970.0000136
CGATAGC150.002212097970.0000150
TCTTTAT401.3096724E-1070.01
Produced by FastQC (version 0.11.5)