##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780252_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48726 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.665476337068505 34.0 34.0 34.0 34.0 34.0 2 33.69523457702253 34.0 34.0 34.0 34.0 34.0 3 33.70602963510241 34.0 34.0 34.0 34.0 34.0 4 33.73482329762344 34.0 34.0 34.0 34.0 34.0 5 33.72002626934285 34.0 34.0 34.0 34.0 34.0 6 37.43742560440011 38.0 38.0 38.0 37.0 38.0 7 37.43744612732422 38.0 38.0 38.0 37.0 38.0 8 37.375302713130566 38.0 38.0 38.0 37.0 38.0 9 37.4077084102943 38.0 38.0 38.0 37.0 38.0 10-11 37.32895168903666 38.0 38.0 38.0 36.0 38.0 12-13 37.28969133522144 38.0 38.0 38.0 36.0 38.0 14-15 37.21220703525839 38.0 38.0 38.0 36.0 38.0 16-17 37.22828674629561 38.0 38.0 38.0 36.0 38.0 18-19 37.140664121824074 38.0 38.0 38.0 35.5 38.0 20-21 37.197256085047 38.0 38.0 38.0 36.0 38.0 22-23 37.21831260517999 38.0 38.0 38.0 36.0 38.0 24-25 37.182305134835616 38.0 38.0 38.0 36.0 38.0 26-27 37.01638755489882 38.0 38.0 38.0 35.5 38.0 28-29 37.01489964290112 38.0 38.0 38.0 35.0 38.0 30-31 37.05146123219636 38.0 38.0 38.0 35.0 38.0 32-33 36.88797561876616 38.0 37.5 38.0 35.0 38.0 34-35 36.970180191273656 38.0 38.0 38.0 35.5 38.0 36-37 36.91923203217995 38.0 37.5 38.0 35.5 38.0 38-39 35.50698805565817 38.0 36.0 38.0 31.0 38.0 40-41 36.15104872142183 38.0 37.0 38.0 33.0 38.0 42-43 36.80409842794401 38.0 37.0 38.0 35.0 38.0 44-45 36.95593728194393 38.0 37.5 38.0 35.5 38.0 46-47 36.876698271969786 38.0 37.5 38.0 35.0 38.0 48-49 36.91226449944588 38.0 37.0 38.0 35.5 38.0 50-51 36.65037146492632 38.0 37.0 38.0 34.0 38.0 52-53 36.627888601567946 38.0 37.0 38.0 34.0 38.0 54-55 36.61198333538563 38.0 37.0 38.0 34.0 38.0 56-57 36.686666256208184 38.0 37.0 38.0 34.0 38.0 58-59 36.44550137503592 38.0 37.0 38.0 34.0 38.0 60-61 36.42989369125313 38.0 37.0 38.0 34.0 38.0 62-63 36.476665435291224 38.0 37.0 38.0 34.0 38.0 64-65 36.26499199605959 38.0 37.0 38.0 33.0 38.0 66-67 35.62335303534047 38.0 37.0 38.0 30.5 38.0 68-69 35.37694454705907 38.0 37.0 38.0 29.5 38.0 70-71 33.882608874112385 38.0 33.5 38.0 18.0 38.0 72-73 31.198764519968805 37.5 24.0 38.0 11.0 38.0 74-75 30.48335590854985 37.0 23.0 38.0 11.0 38.0 76 29.955608915158233 36.0 23.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.023583815839991473 1101 2 0.0963075942379561 1101 3 0.051502683187585774 1101 4 0.0204598863360701 1101 5 0.017957395456939196 1101 6 -0.03843530845105647 1101 7 -0.3072656901854458 1101 8 -0.0015110347367865984 1101 9 0.0024313683904182426 1101 10-11 -0.20213184270569684 1101 12-13 0.048553591820912345 1101 14-15 0.25837294675363864 1101 16-17 0.2619451379175075 1101 18-19 0.03952980579736476 1101 20-21 0.023573660277051545 1101 22-23 0.10337781395700318 1101 24-25 -0.010366462458058834 1101 26-27 -0.06818407146798933 1101 28-29 -0.4730540363129805 1101 30-31 -0.2536587440251026 1101 32-33 -0.28471132747295513 1101 34-35 0.05632502963741359 1101 36-37 0.009792224846499664 1101 38-39 0.07842226439293398 1101 40-41 0.0777892833380065 1101 42-43 0.16900664933629628 1101 44-45 0.22887068417323064 1101 46-47 0.14542560824008177 1101 48-49 0.17737449758445933 1101 50-51 -0.01734886809080649 1101 52-53 -0.18669284040525724 1101 54-55 -0.2426717722691052 1101 56-57 -0.21683888182950994 1101 58-59 -0.019549632420826413 1101 60-61 0.02435566539303835 1101 62-63 0.41282219075746696 1101 64-65 -0.12650628825387145 1101 66-67 0.016677170747136927 1101 68-69 -0.5652719771943779 1101 70-71 0.24528572562721251 1101 72-73 0.732822988982484 1101 74-75 0.43772931656621594 1101 76 0.0682145409797954 1102 1 0.0310300927015561 1102 2 -0.05251799296843984 1102 3 0.06269708598618706 1102 4 -0.0983307183615807 1102 5 -0.4373649433736446 1102 6 -0.10385259980537853 1102 7 -0.07148358123991727 1102 8 -0.12759799125852567 1102 9 0.17044736039441943 1102 10-11 0.1140100863297917 1102 12-13 0.04263155284041176 1102 14-15 0.21699363640881586 1102 16-17 0.1871325442143572 1102 18-19 0.059944598401060034 1102 20-21 -0.034672216784422005 1102 22-23 -0.09149974726238241 1102 24-25 0.15762454203969867 1102 26-27 -0.032302012497474664 1102 28-29 0.07452217558107321 1102 30-31 0.1869959286385665 1102 32-33 0.4667879229018652 1102 34-35 0.14610513958244553 1102 36-37 0.19779822184800366 1102 38-39 0.385093928560849 1102 40-41 0.23743446074929864 1102 42-43 0.13784722904644298 1102 44-45 -0.11400787654640965 1102 46-47 0.06511576316261625 1102 48-49 0.3566348673995563 1102 50-51 -0.08414047228870203 1102 52-53 -0.024099137256825998 1102 54-55 0.15392992688132523 1102 56-57 0.04902818463725822 1102 58-59 0.08657230660965354 1102 60-61 -0.06659885734559623 1102 62-63 0.30877421474547617 1102 64-65 0.32259416152124487 1102 66-67 0.6899155515567372 1102 68-69 0.46006535413994953 1102 70-71 0.0993586891454541 1102 72-73 -0.28214452725939765 1102 74-75 -0.45672345704698003 1102 76 -0.3327349842575842 1103 1 -0.11352526120043649 1103 2 -0.13500438135319115 1103 3 -0.0916132588413987 1103 4 -0.19960113578444805 1103 5 0.07688188058279621 1103 6 -0.1764025090612762 1103 7 -0.061365613907796046 1103 8 0.10003358768884141 1103 9 0.15193556366120475 1103 10-11 0.05234946926989892 1103 12-13 0.08988654376599214 1103 14-15 0.2715308414904811 1103 16-17 0.03039479466808359 1103 18-19 0.1672948706333699 1103 20-21 -0.07768492095864588 1103 22-23 0.02104562478843519 1103 24-25 -0.1306467828241864 1103 26-27 -0.15104066948477168 1103 28-29 0.25916373637961954 1103 30-31 0.15943240776742584 1103 32-33 0.024070137057940144 1103 34-35 0.07958971308516283 1103 36-37 0.09217208754672868 1103 38-39 -0.0766120605498628 1103 40-41 0.10277021392533925 1103 42-43 0.109557755362232 1103 44-45 0.23916816701075305 1103 46-47 -0.05713922776297409 1103 48-49 0.09175283473167894 1103 50-51 0.1545618870579375 1103 52-53 0.284703040601606 1103 54-55 0.0337393642678947 1103 56-57 -0.3707767155442312 1103 58-59 0.23226196178207204 1103 60-61 -0.02088651614776893 1103 62-63 0.04654644705036759 1103 64-65 0.3816458856591751 1103 66-67 -0.037670655339816506 1103 68-69 -4.8364767492614646E-4 1103 70-71 -0.36536454134456875 1103 72-73 -0.3516545091105776 1103 74-75 -0.7326082120379063 1103 76 -0.6375444216441544 1104 1 0.25265805423099863 1104 2 0.15634900210545055 1104 3 -0.036341149997859645 1104 4 -0.03203937423772629 1104 5 0.0488654108370028 1104 6 0.17792079920939585 1104 7 0.2570643896725855 1104 8 0.13498704275507833 1104 9 0.28548371976575027 1104 10-11 -0.25211705418158203 1104 12-13 0.041529042870898536 1104 14-15 0.16993757260397757 1104 16-17 0.05239175134548191 1104 18-19 0.11206751653383407 1104 20-21 -0.23396848701665363 1104 22-23 0.02241064448052299 1104 24-25 -0.05776368221552275 1104 26-27 0.05944089156069765 1104 28-29 -0.259655633473578 1104 30-31 -0.11560787150217777 1104 32-33 -0.11930168535523222 1104 34-35 0.34122594652590976 1104 36-37 0.036855223959193495 1104 38-39 -0.086100066278469 1104 40-41 0.3132965297148189 1104 42-43 0.24464995013487112 1104 44-45 0.23863111008272142 1104 46-47 -0.22221710101754155 1104 48-49 0.21035290633926707 1104 50-51 0.08550531706151787 1104 52-53 0.08268012617267573 1104 54-55 -0.19401752796268568 1104 56-57 -0.2980278791675772 1104 58-59 0.06984699579567888 1104 60-61 -0.09099482262826086 1104 62-63 -0.2934777205113903 1104 64-65 -0.36112622318436394 1104 66-67 -0.4118203152037623 1104 68-69 0.07521900180591246 1104 70-71 0.2667290855798896 1104 72-73 0.5010128736319928 1104 74-75 -0.03579922528530943 1104 76 0.4499063481374428 1105 1 -0.10184994615225662 1105 2 0.08793853932879614 1105 3 0.20592195242620903 1105 4 -0.09915636187785282 1105 5 0.0963407389964317 1105 6 0.2543795517244902 1105 7 0.23876411181495882 1105 8 0.0104301777555591 1105 9 0.11153526714526407 1105 10-11 0.2564821601520322 1105 12-13 0.09187876022263453 1105 14-15 0.268936327035334 1105 16-17 0.1569237049720016 1105 18-19 0.06351429242729267 1105 20-21 0.06705192114661429 1105 22-23 -0.14514229218710284 1105 24-25 -0.23679022240906988 1105 26-27 0.07699861887697779 1105 28-29 0.3751292664018635 1105 30-31 0.07941943519514894 1105 32-33 0.49483551882221377 1105 34-35 0.2691745907723444 1105 36-37 0.40017801786548546 1105 38-39 -0.49062243860768007 1105 40-41 0.10555005084157898 1105 42-43 0.26249511637037415 1105 44-45 0.32256327449778155 1105 46-47 0.037699541695886296 1105 48-49 0.2527980534121852 1105 50-51 -0.28066791279380254 1105 52-53 0.07065559805157307 1105 54-55 -0.18781363795597628 1105 56-57 0.022801861179267746 1105 58-59 0.047402806852488766 1105 60-61 0.25649487747713806 1105 62-63 0.40945415646475425 1105 64-65 0.3050613786169194 1105 66-67 0.18063920381511167 1105 68-69 0.15625282378541527 1105 70-71 0.21446310876662977 1105 72-73 -0.5052868293477886 1105 74-75 0.5199399718019784 1105 76 -0.026906912378031222 1106 1 -0.03902696510047576 1106 2 0.04021333920719883 1106 3 -0.033160021431328346 1106 4 0.11580969344690573 1106 5 -0.09511736212829902 1106 6 0.05253539324845491 1106 7 0.030029690900988726 1106 8 0.22635884327384304 1106 9 0.21554783174147474 1106 10-11 0.3547443954342313 1106 12-13 0.3482877175637995 1106 14-15 0.26916431366011295 1106 16-17 -0.007682638122531671 1106 18-19 0.27227404518846043 1106 20-21 0.3278432880530886 1106 22-23 0.10465505303530875 1106 24-25 0.08198574273431092 1106 26-27 0.011161148008604016 1106 28-29 0.10238126761778688 1106 30-31 0.18662381253834326 1106 32-33 0.29747509730027133 1106 34-35 0.13496646431575954 1106 36-37 -0.09014521650476581 1106 38-39 0.24693962441795492 1106 40-41 0.22696559446301734 1106 42-43 0.0939623369601037 1106 44-45 0.08373461911223501 1106 46-47 0.09902794376296953 1106 48-49 0.3315294345044961 1106 50-51 0.1977746852404465 1106 52-53 0.1515148543085303 1106 54-55 0.1895042260626738 1106 56-57 0.05299742303963484 1106 58-59 0.2871180768899819 1106 60-61 0.294973953371354 1106 62-63 0.046854095668315665 1106 64-65 0.01698761227788026 1106 66-67 0.6493797043724143 1106 68-69 0.6450814791709618 1106 70-71 0.05755764722037071 1106 72-73 0.47057192027223493 1106 74-75 1.17185505945017 1106 76 0.4432015079279772 1107 1 0.11235193178201541 1107 2 0.06309503385148219 1107 3 0.241720074491937 1107 4 0.05186085251964556 1107 5 -0.2897116866686673 1107 6 -0.06711888333028782 1107 7 0.1462367252734893 1107 8 0.09601311985258576 1107 9 0.03534199640974123 1107 10-11 0.27310970318802674 1107 12-13 0.2706248478595654 1107 14-15 -0.06107149769080422 1107 16-17 0.24201760168561748 1107 18-19 0.14445130338191348 1107 20-21 0.36566303225772856 1107 22-23 -0.08714150971449186 1107 24-25 0.16686783705885233 1107 26-27 0.3219125468895001 1107 28-29 0.43259209895271766 1107 30-31 0.09492217384929802 1107 32-33 0.24336838267197436 1107 34-35 0.03262333881615831 1107 36-37 0.24696009924263507 1107 38-39 0.5683075300934135 1107 40-41 0.17093541860370465 1107 42-43 0.14756945126866583 1107 44-45 0.20079097402830115 1107 46-47 0.2655946903656812 1107 48-49 0.34449406280185 1107 50-51 0.11399171161934163 1107 52-53 0.320704502589912 1107 54-55 0.13187532918016842 1107 56-57 0.03346305053764098 1107 58-59 0.5065675173376292 1107 60-61 -0.04025786117701102 1107 62-63 0.35922919558838373 1107 64-65 0.40595144121473226 1107 66-67 0.3824682335490692 1107 68-69 -0.20114154241176863 1107 70-71 -0.01211161736795674 1107 72-73 -0.06382556174215637 1107 74-75 -0.5500424225642213 1107 76 -0.16970816433421376 1108 1 0.03596304289312968 1108 2 0.08392836718481789 1108 3 0.012553407825265595 1108 4 -0.08702803636924727 1108 5 0.036677202220225524 1108 6 -0.3379522166636164 1108 7 0.1254033919401607 1108 8 0.22795756429702863 1108 9 0.05617532974307693 1108 10-11 0.13074859207691958 1108 12-13 -0.25715292991821315 1108 14-15 0.04656739119808151 1108 16-17 -0.06353795386993255 1108 18-19 0.04028463671524207 1108 20-21 0.08788525447994289 1108 22-23 0.2843862680632867 1108 24-25 0.0696456148366309 1108 26-27 -0.10169856422161416 1108 28-29 -0.1160190121583895 1108 30-31 0.018533284960405183 1108 32-33 0.33711838267198146 1108 34-35 0.16456778326060828 1108 36-37 -0.06553990075736493 1108 38-39 -0.12266469212880793 1108 40-41 -0.9401756925074025 1108 42-43 0.06770834015755156 1108 44-45 0.13481875180607972 1108 46-47 -0.1892664207454331 1108 48-49 -0.2318948260870357 1108 50-51 -0.08392495504732267 1108 52-53 0.1540378359232477 1108 54-55 -0.08687467081982447 1108 56-57 0.4015186060931981 1108 58-59 0.24267862844873633 1108 60-61 -0.04720230562145389 1108 62-63 0.09186808447727657 1108 64-65 0.5240069967702894 1108 66-67 0.4970515668824049 1108 68-69 0.5592751242549028 1108 70-71 0.5191383826320433 1108 72-73 -0.7478533395199349 1108 74-75 -1.4215702003419999 1108 76 -1.1072081643342138 1109 1 0.1251965433108424 1109 2 0.013491859248311755 1109 3 0.23827395920371686 1109 4 0.11618833789976435 1109 5 0.21785932419182075 1109 6 0.035063656352257055 1109 7 0.2685216041373195 1109 8 0.20623659520763482 1109 9 0.27223631554090133 1109 10-11 -0.044455042008294754 1109 12-13 0.2153564685780296 1109 14-15 0.010252646001760013 1109 16-17 0.3175188548184522 1109 18-19 0.40225727664840605 1109 20-21 0.33208951513157814 1109 22-23 0.09670351952527767 1109 24-25 0.39801863070964316 1109 26-27 0.22019993201899268 1109 28-29 0.3883930596878926 1109 30-31 0.32907526491028705 1109 32-33 0.09032953555418288 1109 34-35 0.1341009912806541 1109 36-37 0.40420216273470544 1109 38-39 0.7015834281719435 1109 40-41 0.941116078587001 1109 42-43 0.36566677791861935 1109 44-45 0.35946891889045673 1109 46-47 0.2750976143422932 1109 48-49 0.44325659413017604 1109 50-51 0.26452429307298075 1109 52-53 0.1963571759399514 1109 54-55 0.26964161990699154 1109 56-57 0.6137157656587746 1109 58-59 0.6222995557670359 1109 60-61 0.6628540853559883 1109 62-63 0.4229562214864657 1109 64-65 0.153732351824587 1109 66-67 0.18037445744213443 1109 68-69 0.2264900365356013 1109 70-71 0.6773986165501711 1109 72-73 0.6107515059270199 1109 74-75 1.1766962992402874 1109 76 1.183884149784415 1110 1 -0.0877654391100009 1110 2 0.059769525244284694 1110 3 0.10028814960930532 1110 4 0.006477440939050894 1110 5 0.12450975308094314 1110 6 8.584249639227437E-4 1110 7 -0.07109504311461734 1110 8 0.1724020087414715 1110 9 0.23829583052555137 1110 10-11 -0.018165727171911783 1110 12-13 -0.07361419752384535 1110 14-15 -0.23810592648580098 1110 16-17 -0.012090379535038664 1110 18-19 0.16210584172306142 1110 20-21 0.2783195267804217 1110 22-23 -0.12753665838428674 1110 24-25 0.21039287618248181 1110 26-27 -0.06384645154555812 1110 28-29 0.3223123649934152 1110 30-31 0.026602533786700633 1110 32-33 -0.2234743403327215 1110 34-35 0.01213233725121654 1110 36-37 0.4248259051894294 1110 38-39 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1 0.03628159539058373 2116 2 0.02397678716442897 2116 3 -0.12028298361204293 2116 4 -0.24885270507464696 2116 5 -0.13062656944134687 2116 6 -0.20084722176045489 2116 7 -0.07458386595994426 2116 8 -0.48356129401081915 2116 9 -0.5578664643445919 2116 10-11 -0.4477108880454068 2116 12-13 -0.4168432968906899 2116 14-15 -0.13153531012709152 2116 16-17 -0.8172331628403668 2116 18-19 -0.8246172491155477 2116 20-21 -0.7242324825231066 2116 22-23 -0.08605843122315804 2116 24-25 -0.3935934269574588 2116 26-27 -0.07130865596473512 2116 28-29 -0.4095651385600192 2116 30-31 -0.59566778537598 2116 32-33 -0.33327534821487603 2116 34-35 0.0012459178171795315 2116 36-37 -0.5007781780254561 2116 38-39 -0.09858212332146365 2116 40-41 -0.46193282808334857 2116 42-43 -0.4594004773755813 2116 44-45 -0.3532992400389787 2116 46-47 -0.5194460843539943 2116 48-49 -0.6382085365865251 2116 50-51 -0.7665511018363134 2116 52-53 -0.48774988069245495 2116 54-55 -0.10598782066691825 2116 56-57 -0.5582163582289255 2116 58-59 -0.3514536345482071 2116 60-61 -0.263372030392091 2116 62-63 0.2496789917147808 2116 64-65 -0.8138816372766016 2116 66-67 -1.4596890039636676 2116 68-69 -0.9362332855310385 2116 70-71 -0.7021281820978231 2116 72-73 -0.5870480388063797 2116 74-75 0.4169873939495403 2116 76 0.7418616623732284 2117 1 -0.3989409031089508 2117 2 -0.15873778027104635 2117 3 -0.18390297846756454 2117 4 0.06847559810635317 2117 5 -0.02653671262920909 2117 6 0.18593148011727578 2117 7 -0.24563762571300884 2117 8 -0.5705886454641345 2117 9 -0.3369970482423881 2117 10-11 -0.08138147022837927 2117 12-13 -0.7884029299182131 2117 14-15 -0.5406924738071126 2117 16-17 -0.07612881575985853 2117 18-19 -0.2164975543543335 2117 20-21 -0.4212658358627266 2117 22-23 0.12203554116816662 2117 24-25 -0.49116695006472355 2117 26-27 -0.2401331436608629 2117 28-29 -1.2912851076931773 2117 30-31 -0.3067198303043881 2117 32-33 -0.5757515550227268 2117 34-35 -0.1807398491381491 2117 36-37 -0.540390627652485 2117 38-39 -0.4182254397923657 2117 40-41 -0.06988104037863963 2117 42-43 -0.21075090179468248 2117 44-45 -0.5992479667816752 2117 46-47 -0.3832890064152039 2117 48-49 -0.31565001819503635 2117 50-51 -0.20396000177629503 2117 52-53 -0.4188139293934725 2117 54-55 -0.09544164901297592 2117 56-57 -0.3871625984758609 2117 58-59 0.04651429823066877 2117 60-61 -0.635403240200894 2117 62-63 -0.5081448957927179 2117 64-65 -0.5873961704155946 2117 66-67 -0.5470164497323395 2117 68-69 -0.8308144396080266 2117 70-71 -0.8489171729235139 2117 72-73 -0.7808232252935596 2117 74-75 -0.439353170227772 2117 76 -1.042241913036186 2118 1 -0.31196779610913694 2118 2 -0.5681407938129155 2118 3 -0.8084499935352767 2118 4 -0.225916925258133 2118 5 0.004647723762175815 2118 6 -0.4343240987497836 2118 7 -0.11708527019136028 2118 8 -0.05208778717689455 2118 9 -0.5988700217308462 2118 10-11 -0.3677633126849855 2118 12-13 -0.1710559911427012 2118 14-15 -0.34876991038922256 2118 16-17 -0.022438180627304405 2118 18-19 -0.24167398006480312 2118 20-21 -0.029886854363589066 2118 22-23 -0.030310443954853383 2118 24-25 -0.4642262672495363 2118 26-27 -0.4779740744256955 2118 28-29 -0.22224066748719196 2118 30-31 -0.5339752184409576 2118 32-33 -0.809777875831422 2118 34-35 -0.6604747337461916 2118 36-37 -0.6213080413469356 2118 38-39 -0.2527667329451333 2118 40-41 -0.21221367436681504 2118 42-43 -0.3743976689127493 2118 44-45 0.21312090600109457 2118 46-47 -0.3038497540787617 2118 48-49 -0.2981504950212752 2118 50-51 -0.553950465251404 2118 52-53 -0.33908858131031394 2118 54-55 -0.2875549429286721 2118 56-57 -0.3932659744056721 2118 58-59 -0.8519217116873108 2118 60-61 -0.17602883623369792 2118 62-63 -0.2985797613277157 2118 64-65 -0.6859561551571502 2118 66-67 0.2123293446601835 2118 68-69 -1.0761415424117686 2118 70-71 -1.9305498259847376 2118 72-73 -0.3884429086809327 2118 74-75 -0.21266997358462802 2118 76 -0.5432909307741234 2119 1 0.1778281222582052 2119 2 0.023695940880962496 2119 3 -0.12477652414752072 2119 4 0.21285858494594834 2119 5 -0.07698492929905143 2119 6 -0.0975894048722381 2119 7 0.005363709400477035 2119 8 0.009136702619024106 2119 9 0.054191202758950396 2119 10-11 -0.051436782072741494 2119 12-13 0.14016849865321745 2119 14-15 -0.3742801144708494 2119 16-17 0.23266386018902097 2119 18-19 0.013428060751522253 2119 20-21 0.010929472167021004 2119 22-23 0.24519976012677347 2119 24-25 -0.07647116520871577 2119 26-27 0.18529123169675188 2119 28-29 0.5379634141454659 2119 30-31 -0.00846501435931657 2119 32-33 -0.43222685542325934 2119 34-35 -0.07884208068497145 2119 36-37 0.07256950967347109 2119 38-39 -0.20684836559819075 2119 40-41 -0.008132041713750482 2119 42-43 -0.35909154646376606 2119 44-45 -0.16443011440708233 2119 46-47 -0.4314007744869244 2119 48-49 -0.007334168490665149 2119 50-51 0.18074341229961988 2119 52-53 -0.11970082620827327 2119 54-55 -0.33857535109194004 2119 56-57 -0.3473476070587296 2119 58-59 -0.5457992627077175 2119 60-61 -0.7576614892949252 2119 62-63 -0.10980425112362724 2119 64-65 -0.3594255429122555 2119 66-67 -0.2570584104418572 2119 68-69 -0.05063133833014177 2119 70-71 0.6051644597295436 2119 72-73 1.3819652545843724 2119 74-75 1.3842688019255753 2119 76 -0.2575766450598387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 2.0 22 2.0 23 8.0 24 21.0 25 36.0 26 51.0 27 112.0 28 203.0 29 389.0 30 596.0 31 960.0 32 1479.0 33 2482.0 34 4311.0 35 7191.0 36 8353.0 37 22529.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.448343800024627 12.100316053031236 13.45072445922095 45.00061568772319 2 15.650781923408447 15.78418093009892 42.13972006731519 26.42531707917744 3 16.221319213561546 13.08131182530887 38.68571194023724 32.011657020892336 4 13.641587653408857 30.072240692853917 28.750564380412918 27.535607273324302 5 14.105405738209582 25.44637359931043 45.28177974797849 15.166440914501496 6 25.79321101670566 41.16898575709067 14.714936584164512 18.322866642039155 7 23.693715880638674 21.653737224479745 21.16323933834093 33.48930755654065 8 20.124779378565858 41.65948364322949 12.204982965972992 26.01075401223166 9 18.5260435906908 14.907852070763042 11.71858966465542 54.84751467389074 10-11 24.302220580388294 29.165127447358703 21.861018757952632 24.671633214300375 12-13 22.792759512375323 14.833969543980627 27.363214710832 35.010056232812055 14-15 19.501908631941877 12.812461519517301 17.96782005500144 49.71780979353939 16-17 31.321060624717813 23.834297910766324 13.099782457004475 31.74485900751139 18-19 36.12342486557485 20.993925214464557 13.237286048516191 29.645363871444403 20-21 25.116980667405493 15.964782662233715 13.486639576406848 45.43159709395395 22-23 22.71990313179822 24.85326109264048 12.946886672413086 39.479949103148215 24-25 31.898780938308093 25.14058203012765 16.579444239215206 26.381192792349058 26-27 34.07113245495218 31.567335713992527 13.729836227065631 20.631695603989655 28-29 31.792061732955712 21.51931207158396 14.393752821902064 32.29487337355826 30-31 27.822928210811476 15.79957312317859 32.482658129130236 23.894840536879695 32-33 19.582974182161475 33.4123465911423 24.774247834831506 22.230431391864713 34-35 19.77383737634938 30.995772277634114 16.81648401264212 32.413906333374385 36-37 32.34104995279728 27.20313590280343 16.43578377047162 24.02003037392768 38-39 22.416163855026063 21.30176907605796 27.68542461929976 28.596642449616223 40-41 20.315642572753767 13.45072445922095 30.286705249763983 35.94692771826129 42-43 30.746418749743466 13.022821491606123 28.038418913926854 28.192340844723557 44-45 20.57423141649222 24.20781512949965 33.0675614661577 22.15039198785043 46-47 27.83626811148052 16.934490826252922 26.984566761072116 28.244674301194433 48-49 27.917333661700116 13.950457661207569 19.314123876369905 38.81808480072241 50-51 23.163198292492716 14.747773262734473 28.526864507655052 33.56216393711776 52-53 15.709272257111193 14.841152567417806 43.448056479087136 26.00151869638386 54-55 17.426014858597053 16.226449944588104 34.251734187086974 32.095801009727865 56-57 26.415055617124327 18.43163813980216 22.829700775766533 32.32360546730698 58-59 19.123260682181996 19.269999589541516 35.09420022164758 26.512539506628908 60-61 26.57821286376883 27.70286910479005 24.16984771990313 21.54907031153799 62-63 18.47781471904117 44.11197307392357 15.550219595287937 21.859992611747323 64-65 15.555350326314493 47.92410622665517 14.826786520543447 21.693756926486884 66-67 16.021220703525838 47.19143783606288 14.558962360957189 22.22837909945409 68-69 15.809834585231705 47.47465418872881 14.658498542872389 22.0570126831671 70-71 16.33316914994048 44.69585026474572 15.853958872060092 23.117021713253706 72-73 16.42962689323975 39.30447810203998 17.972950786027994 26.292944218692277 74-75 16.362927389894512 37.47280712555925 19.157123506957273 27.007141977588965 76 16.88626195460329 37.52616672823544 18.792841604071747 26.79472971308952 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 1.5 17 3.0 18 2.0 19 2.0 20 1.5 21 0.0 22 3.0 23 9.5 24 16.5 25 20.0 26 18.5 27 32.0 28 53.0 29 59.0 30 77.5 31 112.0 32 178.5 33 229.0 34 264.5 35 331.5 36 372.0 37 381.0 38 540.5 39 2401.5 40 5029.0 41 5370.0 42 4785.0 43 3906.0 44 2486.0 45 1666.0 46 1387.0 47 1252.0 48 1074.5 49 999.0 50 966.0 51 852.0 52 703.0 53 679.0 54 690.0 55 690.0 56 733.5 57 934.5 58 1092.0 59 1138.5 60 1215.5 61 1297.0 62 1348.0 63 1459.5 64 1600.0 65 1647.5 66 1703.5 67 1741.0 68 1719.0 69 1575.0 70 1373.0 71 1293.0 72 1161.5 73 886.0 74 589.5 75 437.0 76 338.0 77 159.5 78 52.0 79 24.0 80 13.5 81 2.0 82 1.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48726.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.61934080367771 #Duplication Level Percentage of deduplicated Percentage of total 1 71.32698357970952 33.96543939580511 2 19.54488643709865 18.614292164347574 3 5.727707624014136 8.182489841152567 4 1.8704477869241047 3.562779624840948 5 0.7197345170883076 1.7136641628699256 6 0.3059949144507176 0.8742765669252556 7 0.12929362582424686 0.43098140623075976 8 0.07757617549454812 0.2955301071296638 9 0.06464681291212343 0.27705947543405984 >10 0.15084256346162134 1.356565283421582 >50 0.03447830021979917 1.0528260066494275 >100 0.025858725164849373 1.976357591429627 >500 0.004309787527474896 1.0938718548618807 >1k 0.012929362582424686 11.57287690350121 >5k 0.004309787527474896 15.030989615400403 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 7324 15.030989615400403 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 2007 4.11895086811969 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 1932 3.9650289373229897 No Hit CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG 1700 3.488897098058531 Illumina PCR Primer Index 9 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 533 1.0938718548618807 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 299 0.613635430776177 Illumina PCR Primer Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT 179 0.36736034150145713 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 130 0.2667980133809465 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT 125 0.25653655132783315 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 124 0.2544842589172105 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 106 0.21754299552600256 No Hit CAGCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGC 81 0.16623568526043592 No Hit TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT 79 0.16213110043919057 Illumina PCR Primer Index 9 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 69 0.1416081763329639 No Hit CTTATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 64 0.1313467142798506 No Hit ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT 60 0.12313754463735993 Illumina PCR Primer Index 9 (95% over 24bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT 55 0.1128760825842466 No Hit CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 54 0.11082379017362395 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG 51 0.10466691294175594 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006156877231867997 0.0 2 0.0 0.0 0.0 0.012313754463735994 0.0 3 0.0 0.0 0.0 0.012313754463735994 0.0 4 0.0 0.0 0.0 0.026679801338094652 0.0 5 0.0 0.0 0.0 0.030784386159339983 0.0 6 0.0 0.0 0.0 0.030784386159339983 0.0 7 0.0 0.0 0.0 0.03488897098058531 0.0 8 0.0 0.0 0.0 0.03488897098058531 0.0 9 0.0 0.0 0.0 0.05130731026556664 0.0 10 0.0 0.0 0.0 0.057464187497434635 0.0 11 0.0 0.0 0.0 0.057464187497434635 0.0 12 0.0 0.0 0.0 0.06772564955054797 0.0 13 0.0 0.0 0.0 0.0718302343717933 0.0 14 0.0 0.0 0.0 0.07593481919303863 0.0 15 0.0 0.0 0.0 0.07593481919303863 0.0 16 0.0 0.0 0.0 0.08209169642490662 0.0 17 0.0 0.0 0.0 0.08414398883552929 0.0 18 0.0 0.0 0.0 0.08414398883552929 0.0 19 0.0 0.0 0.0 0.08414398883552929 0.0 20 0.0 0.0 0.0 0.09235315847801995 0.0 21 0.0 0.0 0.0 0.09440545088864262 0.0 22 0.0 0.0 0.0 0.10466691294175594 0.0 23 0.0 0.0 0.0 0.11492837499486927 0.0 24 0.0 0.0 0.0 0.12929442186922793 0.0 25 0.0 0.0 0.0 0.1313467142798506 0.0 26 0.0 0.0 0.0 0.16213110043919057 0.0 27 0.0 0.0 0.0 0.21959528793662522 0.0 28 0.0 0.0 0.0 0.35915117185896644 0.0 29 0.0 0.0 0.0 0.5192299798875344 0.0 30 0.0 0.0 0.0 0.7018840044329516 0.0 31 0.0 0.0 0.0 0.8763288593358782 0.0 32 0.0 0.0 0.0 1.0487214218281822 0.0 33 0.0 0.0 0.0 1.2970488035135246 0.0 34 0.0 0.0 0.0 1.7444485490292656 0.0 35 0.0 0.0 0.0 2.312933546771744 0.0 36 0.0 0.0 0.0 3.0538111070065264 0.0 37 0.0 0.0 0.0 4.008127077946066 0.0 38 0.0 0.0 0.0 4.86598530558634 0.0 39 0.0 0.0 0.0 5.768993966260313 0.0 40 0.0 0.0 0.0 6.633009071132455 0.0 41 0.0 0.0 0.0 7.490867298772729 0.0 42 0.0 0.0 0.0 8.387719082214835 0.0 43 0.0 0.0 0.0 9.364610269671223 0.0 44 0.0 0.0 0.0 10.253252883470838 0.0 45 0.0 0.0 0.0 11.148052374502319 0.0 46 0.0 0.0 0.0 11.977178508393877 0.0 47 0.0 0.0 0.0 12.754997332019867 0.0 48 0.0 0.0 0.0 13.463038213684685 0.0 49 0.0 0.0 0.0 14.11977178508394 0.0 50 0.0 0.0 0.0 14.66773385872019 0.0 51 0.0 0.0 0.0 15.246480318515783 0.0 52 0.0 0.0 0.0 15.958625785001846 0.0 53 0.0 0.0 0.0 16.664614374256043 0.0 54 0.0 0.0 0.0 17.284406682264088 0.0 55 0.0 0.0 0.0 17.959610885358945 0.0 56 0.0 0.0 0.0 18.50962525140582 0.0 57 0.0 0.0 0.0 19.11710380495013 0.0 58 0.0 0.0 0.0 19.62607232278455 0.0 59 0.0 0.0 0.0 20.122727086155237 0.0 60 0.0 0.0 0.0 20.719944177646433 0.0 61 0.0 0.0 0.0 21.22686040307023 0.0 62 0.0 0.0 0.0 21.809711447687068 0.0 63 0.0 0.0 0.0 22.2837909945409 0.0 64 0.0 0.0 0.0 22.862537454336493 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTACC 15 0.0022120979 70.00001 32 GGCAAGA 15 0.0022120979 70.00001 12 GTGGCAA 15 0.0022120979 70.00001 10 TACATTT 15 0.0022120979 70.00001 26 ATCGTAT 15 0.0022120979 70.00001 39 TGGCAAG 15 0.0022120979 70.00001 11 ACGATAG 15 0.0022120979 70.00001 49 TCTACCC 15 0.0022120979 70.00001 33 TACGATA 15 0.0022120979 70.00001 48 CTACGAT 15 0.0022120979 70.00001 47 GAAACTT 15 0.0022120979 70.00001 63 GAAAACT 15 0.0022120979 70.00001 42 CTACATT 15 0.0022120979 70.00001 25 TGGGCTA 15 0.0022120979 70.00001 21 GCTACAT 15 0.0022120979 70.00001 24 CATTTTC 15 0.0022120979 70.00001 28 TTTCTAC 15 0.0022120979 70.00001 31 ACCCCAG 15 0.0022120979 70.00001 36 CGATAGC 15 0.0022120979 70.00001 50 TCTTTAT 40 1.3096724E-10 70.0 1 >>END_MODULE