FastQCFastQC Report
Wed 25 May 2016
SRR1780251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273648
Sequences flagged as poor quality0
Sequence length50
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGC207.8805577E-443.9606931
TTTATAG300.00577276329.2964173
CTACACA551.6028594E-423.9697974
TGTATAA500.002577804321.99643311
AATGTGT703.234935E-521.97231333
CCCAATG755.4442426E-520.53751830
ATCTTAG550.004498102219.9821341
CAATGTG809.043253E-519.22577332
GTACTGG953.3572645E-416.1960471
CCAATGT953.3666327E-416.19012631
AGATCTC1552.6226735E-715.62466440
TCTGACG5500.015.59747111
TAGATCT1301.3515673E-515.24224339
CTGCCGA4750.014.84308918
TGACGCT4800.014.67502813
CCTACGA900.00437345914.6535642
AGATGTG900.004384114.6482096
ATCTGAC5750.014.15422610
TGCCGAC4900.013.93906119
CGACGAA1901.7392267E-713.89248522
Produced by FastQC (version 0.11.5)