FastQCFastQC Report
Wed 25 May 2016
SRR1780251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273648
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT191146.984885692568556No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC56952.081140735543472No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG51821.8936736245103196No Hit
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG29971.0952025960357832RNA PCR Primer, Index 36 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC10630.38845524177044966No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT4910.17942758580366017RNA PCR Primer, Index 15 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT351.413282E-744.1075481
ACCGTAT207.850609E-443.99478
ATCGGTC207.850609E-443.994726
CAGCTTA301.2799782E-436.756291
TCTTTAT1800.036.756291
GCTTATA301.2966324E-436.6756553
TAATACC250.00234679835.208634
TATACAA250.00234679835.208632
ATACAAC250.00234679835.208633
TTTCCCC250.002351011835.1957616
TCTTCTG4150.034.4599844
ATGCCGT26000.034.01750644
CTTTATA1950.033.854452
GTCTTCT4300.033.25788543
CCGTCTT4700.030.4274341
GATCGGT300.005740727329.329825
CAACTGA300.005740727329.329832
TTTATAC2200.029.0071093
CGTCTTC5050.028.31859842
TATGCCG31550.028.10317843
Produced by FastQC (version 0.11.5)