FastQCFastQC Report
Wed 25 May 2016
SRR1780250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75241
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGA150.002216805570.019
AACCGTG200.00692692452.50000432
CAGAACT200.00692692452.5000044
GAACTAA200.00692692452.5000046
CGTCTTA200.00692692452.5000046
AGTTATG200.00692692452.50000418
GGGTTAG200.00692692452.5000041
ACCGTGT200.00692692452.50000433
GAACCGT200.00692692452.50000431
TTCGAAA200.00692692452.50000430
AATGAGC305.8442284E-446.66666432
CGGTGTA350.001248300940.00000434
CACAGAA350.001248300940.0000042
TGCTCTC400.00240513235.00000440
ACAGAAC400.00240513235.0000043
TCGGTGG652.0781494E-532.30769345
CTTAATA552.9671166E-431.818182
ATCTCGG450.004283150731.11110942
CAGGAAT450.004283150731.11110913
AGATCTC703.4481007E-530.00000240