##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780250_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75241 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.206576201804868 34.0 27.0 34.0 12.0 34.0 2 29.205699020480854 34.0 27.0 34.0 12.0 34.0 3 29.05777435174971 34.0 27.0 34.0 11.0 34.0 4 28.884291808987122 34.0 27.0 34.0 11.0 34.0 5 29.00676492869579 34.0 27.0 34.0 11.0 34.0 6 31.53323321061655 37.0 27.0 38.0 11.0 38.0 7 31.942956632686965 37.0 28.0 38.0 12.0 38.0 8 31.846067968261984 37.0 28.0 38.0 11.0 38.0 9 32.199425844951556 38.0 31.0 38.0 12.0 38.0 10-11 31.88326843077577 37.5 29.5 38.0 11.0 38.0 12-13 31.9168272617323 37.0 29.5 38.0 11.0 38.0 14-15 31.77561435919246 37.0 27.5 38.0 11.0 38.0 16-17 31.840266609959997 38.0 29.5 38.0 11.0 38.0 18-19 31.833980143804574 38.0 28.0 38.0 11.0 38.0 20-21 31.831986549886366 38.0 29.5 38.0 11.0 38.0 22-23 31.796367671881022 38.0 29.0 38.0 11.0 38.0 24-25 31.81290121077604 38.0 29.5 38.0 11.0 38.0 26-27 31.791529883972835 38.0 28.0 38.0 11.0 38.0 28-29 31.825520660278308 38.0 29.5 38.0 11.0 38.0 30-31 31.836372456506425 38.0 28.0 38.0 11.0 38.0 32-33 31.895462580242153 38.0 29.5 38.0 11.0 38.0 34-35 31.843948113395623 38.0 28.5 38.0 11.0 38.0 36-37 31.786300022594062 38.0 27.0 38.0 11.0 38.0 38-39 31.752023497826983 38.0 27.0 38.0 11.0 38.0 40-41 31.79350354195186 38.0 27.0 38.0 11.0 38.0 42-43 31.91835568373626 38.0 29.0 38.0 11.0 38.0 44-45 31.97858880131843 38.0 31.0 38.0 11.0 38.0 46-47 31.882125436929336 38.0 29.0 38.0 11.0 38.0 48-49 31.80668784306429 38.0 27.0 38.0 11.0 38.0 50-51 31.735609574567057 38.0 27.0 38.0 11.0 38.0 52-53 31.817978229954413 38.0 27.0 38.0 11.0 38.0 54-55 31.79854733456493 38.0 27.0 38.0 11.0 38.0 56-57 31.735270663600964 38.0 27.0 38.0 11.0 38.0 58-59 31.78599433819327 38.0 27.0 38.0 11.0 38.0 60-61 31.79433420608445 38.0 27.0 38.0 11.0 38.0 62-63 31.684380856182134 37.5 27.0 38.0 11.0 38.0 64-65 31.58186361159474 37.0 27.0 38.0 11.0 38.0 66-67 31.511954918196196 37.0 27.0 38.0 11.0 38.0 68-69 31.43312821467019 37.0 27.0 38.0 11.0 38.0 70-71 31.357046025438258 37.0 27.0 38.0 11.0 38.0 72-73 31.299178639305698 37.0 26.0 38.0 11.0 38.0 74-75 31.31022979492564 37.0 26.0 38.0 11.0 38.0 76 29.853749950160154 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2925222624294115 1101 2 -0.9857116958408056 1101 3 0.1472752033815894 1101 4 0.3573196224239936 1101 5 -0.12525331459259803 1101 6 0.6761192019449638 1101 7 -0.34456707147231214 1101 8 -1.073889709155985 1101 9 0.17671338926819402 1101 10-11 -0.30899582002201953 1101 12-13 -0.05777688558759664 1101 14-15 -0.07184079992576287 1101 16-17 0.11395920183919017 1101 18-19 0.18908514140569466 1101 20-21 0.4736162354902582 1101 22-23 -0.4771132484195171 1101 24-25 -0.01087696402982985 1101 26-27 -0.29704316940940245 1101 28-29 -0.5360529681952144 1101 30-31 -0.11075218098739725 1101 32-33 -0.6187673434262102 1101 34-35 -0.8160236528933567 1101 36-37 0.009680250087644282 1101 38-39 0.4321768608498857 1101 40-41 0.09689326640424412 1101 42-43 -0.36699188178855735 1101 44-45 0.2556407213360714 1101 46-47 0.041595184171406174 1101 48-49 0.17352394838668772 1101 50-51 -0.6722010960234783 1101 52-53 0.275923536426788 1101 54-55 -0.5315061310415246 1101 56-57 -0.45466397655582824 1101 58-59 -0.11155353733677842 1101 60-61 -0.4702687441745468 1101 62-63 -0.25427021916166126 1101 64-65 -0.2628290538770415 1101 66-67 -0.09941901058863678 1101 68-69 -0.003201179982603719 1101 70-71 -0.16423228053316308 1101 72-73 0.05944652182360599 1101 74-75 -0.7881760374854743 1101 76 -1.8508154043857523 1102 1 -0.3976491246268061 1102 2 -0.2969605319674322 1102 3 -0.4812164167301418 1102 4 -1.2403002565331782 1102 5 -0.7650345063989299 1102 6 -0.5458314498241243 1102 7 0.19308423206586056 1102 8 0.05145945285518749 1102 9 -0.3638766945306884 1102 10-11 -1.0999059689978132 1102 12-13 -0.593827863241227 1102 14-15 -0.3472568846557458 1102 16-17 -0.33359375439917116 1102 18-19 -1.1286231919276375 1102 20-21 -0.9329829265209177 1102 22-23 -0.7941378061476385 1102 24-25 -0.5776687470838375 1102 26-27 -1.1592696591673146 1102 28-29 -1.1225869532976347 1102 30-31 -0.9343671716764064 1102 32-33 -1.0200272363498577 1102 34-35 -0.6974249564315329 1102 36-37 -1.132821495722414 1102 38-39 -0.9036588002301897 1102 40-41 -1.095364065997991 1102 42-43 -1.1949423007829694 1102 44-45 -0.6964367926304078 1102 46-47 -1.1057220877094167 1102 48-49 -0.11379274155744667 1102 50-51 -0.7947424135281338 1102 52-53 -1.21208856785627 1102 54-55 -0.8432205165163857 1102 56-57 -1.1781713089580634 1102 58-59 -1.8520679693665691 1102 60-61 -1.216835317731345 1102 62-63 -1.3235497815452746 1102 64-65 -1.2891325920520842 1102 66-67 -1.064822874648229 1102 68-69 -1.3392405189025318 1102 70-71 -1.2438557674977844 1102 72-73 -1.225280549870991 1102 74-75 -1.6191961724947816 1102 76 -0.7320712237526052 1103 1 -0.30361122680436026 1103 2 -0.12262531421199085 1103 3 -0.23247269813717608 1103 4 -0.9978641175047009 1103 5 -0.8097371864659308 1103 6 -1.5350483677470734 1103 7 -0.5704731381183912 1103 8 -0.3741058737779781 1103 9 -0.33270521211862913 1103 10-11 -0.5164156004885996 1103 12-13 0.11039638298992926 1103 14-15 -0.7558754985585914 1103 16-17 -0.3021998054879518 1103 18-19 -0.5018696324636522 1103 20-21 -0.482449006922927 1103 22-23 0.14895002886073527 1103 24-25 -0.8052258031977395 1103 26-27 -0.36872841126111666 1103 28-29 -0.4406878745706706 1103 30-31 -0.6761489974720547 1103 32-33 -1.0831422907887216 1103 34-35 -0.7006703500663711 1103 36-37 -0.8678638952199016 1103 38-39 -1.145754696377594 1103 40-41 -0.8943197820783908 1103 42-43 -1.4919743359588473 1103 44-45 -1.117369581575133 1103 46-47 -0.5216820960846249 1103 48-49 -0.5712754676880998 1103 50-51 -0.741415885052426 1103 52-53 -0.647913525980222 1103 54-55 -1.0436759185264357 1103 56-57 -0.41823883408369156 1103 58-59 -1.0655834635039234 1103 60-61 -0.9081565070110749 1103 62-63 -1.0163628049958575 1103 64-65 -0.979877985686926 1103 66-67 -0.9873783604103714 1103 68-69 -0.7946163564234645 1103 70-71 -0.8125717432096735 1103 72-73 -0.8910180917470392 1103 74-75 -1.0748468215735087 1103 76 -0.6235337446906293 1104 1 -0.11904103283936607 1104 2 -0.2849587203732291 1104 3 -0.7638251123823174 1104 4 -0.14390834832061827 1104 5 -0.9730659073651111 1104 6 -0.40905608750528444 1104 7 -0.8650377486104652 1104 8 -1.3600743635699324 1104 9 -0.8151901003277899 1104 10-11 -0.29057625885288374 1104 12-13 -0.36669924005282084 1104 14-15 -1.0547976455253085 1104 16-17 -0.7558084283122142 1104 18-19 -0.5935235542464774 1104 20-21 -0.7667691584049763 1104 22-23 -0.93691407426358 1104 24-25 -0.9070392180983298 1104 26-27 -0.9413499731769797 1104 28-29 -0.9159142479836291 1104 30-31 -0.6036002634638464 1104 32-33 -0.7007714996348859 1104 34-35 -0.9622196424218714 1104 36-37 -0.9762318218093675 1104 38-39 -1.4041116987809161 1104 40-41 -0.7212321215535482 1104 42-43 -0.8651566727636464 1104 44-45 -1.0690968167849988 1104 46-47 -0.9909123051007214 1104 48-49 -0.7211674396250807 1104 50-51 -0.9378206140112297 1104 52-53 -0.6640813214794576 1104 54-55 -1.0715568691733885 1104 56-57 -1.241388398330045 1104 58-59 -0.80813680994628 1104 60-61 -0.5726626124173322 1104 62-63 -0.563057631786819 1104 64-65 -0.6109924954337274 1104 66-67 -0.9346145413148967 1104 68-69 -0.8456565817044641 1104 70-71 -0.6300650066282145 1104 72-73 -0.9376522286149545 1104 74-75 -1.0102363294996124 1104 76 -0.1710054024965686 1105 1 0.2926870964953068 1105 2 0.25249285087085127 1105 3 0.5781895238639159 1105 4 0.5153206180542824 1105 5 0.46908265531724425 1105 6 0.40569495281613754 1105 7 0.3596477634190016 1105 8 0.67460301721162 1105 9 0.3228497906178234 1105 10-11 0.25168231483057113 1105 12-13 -0.6960865266075622 1105 14-15 -0.40570882359963534 1105 16-17 -0.2639516339371255 1105 18-19 0.07522375526707847 1105 20-21 0.17314331110284442 1105 22-23 -0.00966482759978149 1105 24-25 -0.5609350424633739 1105 26-27 0.10676788208681032 1105 28-29 0.5128297133690296 1105 30-31 -0.07555941590083037 1105 32-33 0.2995633164554157 1105 34-35 0.5366922052846412 1105 36-37 0.5088090735845121 1105 38-39 0.421114302080035 1105 40-41 0.11402640429903954 1105 42-43 0.8067594318902991 1105 44-45 0.3030990984587021 1105 46-47 0.009015948594214507 1105 48-49 0.33182916438315146 1105 50-51 0.2849038323464512 1105 52-53 0.29018040904141884 1105 54-55 0.14517676071133678 1105 56-57 0.4090038148043149 1105 58-59 0.4609477072010826 1105 60-61 0.5920549462950575 1105 62-63 0.6299877597088539 1105 64-65 0.38124694590170805 1105 66-67 0.5505339735365915 1105 68-69 0.639049497599121 1105 70-71 0.8701861054395152 1105 72-73 0.5916800524392372 1105 74-75 0.19781599747221534 1105 76 0.10667671354112329 1106 1 0.15996305486037343 1106 2 -0.9262073268392292 1106 3 -0.10942154493526957 1106 4 0.2540106409027203 1106 5 -0.220483224347646 1106 6 -0.3711519626446389 1106 7 -0.5166914089597796 1106 8 -0.4482200343243008 1106 9 -0.37148887401786723 1106 10-11 -0.5337200715619126 1106 12-13 -0.25945286324122563 1106 14-15 -0.4497809231172809 1106 16-17 -0.3678485620914813 1106 18-19 -0.3442481919276368 1106 20-21 -0.177694464982455 1106 22-23 0.03554969385236362 1106 24-25 -0.15887868298127117 1106 26-27 -0.3083882489109051 1106 28-29 -0.393580543041228 1106 30-31 -0.9902966588558968 1106 32-33 -0.33993909532421895 1106 34-35 -0.38748905899563724 1106 36-37 -0.10521732905574765 1106 38-39 0.1080399177185285 1106 40-41 -0.06311246343388888 1106 42-43 0.06122757101190501 1106 44-45 -0.23401692083553627 1106 46-47 -0.43929580565813353 1106 48-49 -0.4699225492497554 1106 50-51 -0.08717029814351918 1106 52-53 -0.1906142088819074 1106 54-55 -0.46862115754202804 1106 56-57 -0.47620816793242327 1106 58-59 -0.55351027705888 1106 60-61 -0.2272519843980092 1106 62-63 -0.9031170892375826 1106 64-65 -0.4333633612828578 1106 66-67 -0.44743505413540774 1106 68-69 -0.12008987787688952 1106 70-71 -0.2530304469849618 1106 72-73 -0.6688302293581749 1106 74-75 0.14085991724880742 1106 76 0.5337140326576488 1107 1 1.5450592087065296 1107 2 1.666581134699232 1107 3 0.9406585832698582 1107 4 1.8013664101334896 1107 5 0.8599654936010701 1107 6 1.9020852168425435 1107 7 2.2764175653991963 1107 8 1.7129177861885232 1107 9 1.3444566388026473 1107 10-11 1.0198856976688582 1107 12-13 1.5962763034254444 1107 14-15 1.4105556153442613 1107 16-17 1.749739578934161 1107 18-19 1.4468976414056947 1107 20-21 1.2206629068124109 1107 22-23 1.6485705271856936 1107 24-25 1.9327479195828268 1107 26-27 1.6975011741660175 1107 28-29 1.851371380035694 1107 30-31 1.544799494990258 1107 32-33 1.5685144303168066 1107 34-35 1.359866710235135 1107 36-37 1.445303504277586 1107 38-39 1.5650911997698032 1107 40-41 1.6754692673353446 1107 42-43 1.9222451992170306 1107 44-45 1.683771540702928 1107 46-47 1.782298745623919 1107 48-49 1.727353091775889 1107 50-51 2.056820086471859 1107 52-53 1.7931197654770656 1107 54-55 1.6984461501502857 1107 56-57 1.8765161910419366 1107 58-59 1.5906403639667666 1107 60-61 1.7831646822686622 1107 62-63 1.5175960517880611 1107 64-65 1.5780549079479158 1107 66-67 1.7841354586851033 1107 68-69 2.067009481097468 1107 70-71 1.4254150658355513 1107 72-73 1.9335736167956732 1107 74-75 2.070907994171886 1107 76 1.856470442914059 1108 1 -0.44819424552490617 1108 2 -0.710955566509746 1108 3 -1.0848433597353235 1108 4 -0.04100729452972374 1108 5 -0.20685229569081187 1108 6 0.5889915208149077 1108 7 0.05248621824893718 1108 8 -0.6315068683883354 1108 9 -0.533970606447788 1108 10-11 -0.07243403462820908 1108 12-13 -0.6262922414795682 1108 14-15 -0.3127010772291463 1108 16-17 -0.09728924230936187 1108 18-19 -0.054438174656482374 1108 20-21 -0.002445361754077169 1108 22-23 0.4084339770993388 1108 24-25 -0.6663318515743377 1108 26-27 -0.27598490096351824 1108 28-29 -0.18535681512837954 1108 30-31 0.05888628255502226 1108 32-33 -0.10704418367282997 1108 34-35 0.5563531091988629 1108 36-37 0.03796056386307711 1108 38-39 -0.2857291801956485 1108 40-41 -0.056868275842550275 1108 42-43 0.31274876139319474 1108 44-45 -0.09337655946978884 1108 46-47 0.030639095364850988 1108 48-49 -0.042022987671437306 1108 50-51 0.20089931790536752 1108 52-53 0.12934062039934702 1108 54-55 0.8226635508411242 1108 56-57 0.4667040148761359 1108 58-59 0.42292123616020305 1108 60-61 -0.1780291864705461 1108 62-63 0.46485155696940694 1108 64-65 -0.046688723312882985 1108 66-67 -0.18331920452733286 1108 68-69 -0.05990653790080103 1108 70-71 -0.30394050411263507 1108 72-73 0.23846351316872827 1108 74-75 0.0668330805276689 1108 76 -0.3955805069995826 1109 1 -0.014395919498600307 1109 2 0.05327985264795032 1109 3 0.059809224295499064 1109 4 -0.24342525653317537 1109 5 -0.39997040383482485 1109 6 0.03397624248356834 1109 7 0.016641924373555383 1109 8 -0.46360464970891613 1109 9 -0.22789913042812415 1109 10-11 0.09192095407911438 1109 12-13 -0.7107349145232753 1109 14-15 -0.36003733337369326 1109 16-17 -0.09092548516840182 1109 18-19 -0.21091485859430392 1109 20-21 -0.3135919008798922 1109 22-23 -0.043937485634817364 1109 24-25 -0.07426329836588508 1109 26-27 -0.005824146346803616 1109 28-29 0.11411176465108497 1109 30-31 0.012267443708207537 1109 32-33 0.052368596983473736 1109 34-35 0.10481863331205687 1109 36-37 0.17170574786733184 1109 38-39 0.14393735361596072 1109 40-41 -0.08106118138260854 1109 42-43 -0.2592852495009197 1109 44-45 -0.08273486955348375 1109 46-47 -0.4162188825812123 1109 48-49 -0.13915331848052404 1109 50-51 -0.07434978532300818 1109 52-53 -0.5034347217024191 1109 54-55 -0.09939038831125657 1109 56-57 -0.3608235525478065 1109 58-59 -0.1560743796229822 1109 60-61 -0.10930326644929167 1109 62-63 0.0840623979419064 1109 64-65 0.0025340746145801063 1109 66-67 -0.08589659259694926 1109 68-69 -0.6226539804409903 1109 70-71 -0.388927882882399 1109 72-73 -0.20216356269150637 1109 74-75 0.17932145571034752 1109 76 0.06191916086277871 1110 1 0.3992258753731939 1110 2 0.7186644680325678 1110 3 0.9458669166031903 1110 4 0.1555330768001575 1110 5 -0.1348261730655942 1110 6 0.5479185501758721 1110 7 0.6462092320658606 1110 8 -0.42770721381148036 1110 9 0.9382066388026473 1110 10-11 0.49123986433552247 1110 12-13 0.640547136758773 1110 14-15 0.2829514486775899 1110 16-17 0.4398437456008253 1110 18-19 0.9364809747390233 1110 20-21 0.18160040681241796 1110 22-23 0.5391955271856936 1110 24-25 0.41712291958282677 1110 26-27 0.48135534083268894 1110 28-29 1.0362672133690296 1110 30-31 0.8208411616569222 1110 32-33 0.7091394303168066 1110 34-35 0.7322625435684635 1110 36-37 0.30988683761091806 1110 38-39 1.0755078664364746 1110 40-41 0.5322401006686732 1110 42-43 0.4352660325503592 1110 44-45 0.4546048740362565 1110 46-47 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-0.7514708445523048 2116 34-35 -0.21825828976486505 2116 36-37 -0.6457059852512081 2116 38-39 -0.29245679324938934 2116 40-41 -1.2180325869403994 2116 42-43 0.1245112332484446 2116 44-45 -0.18015734236862713 2116 46-47 -0.2304221313394308 2116 48-49 -0.4559475190443578 2116 50-51 0.013326412824394396 2116 52-53 -0.25604581043916497 2116 54-55 0.059839038457436544 2116 56-57 -0.17534899220413536 2116 58-59 -0.35506753306639993 2116 60-61 -0.9837964870158089 2116 62-63 -0.5050233853847139 2116 64-65 -0.5419685431864671 2116 66-67 -0.19639464602693835 2116 68-69 -0.13458846829170668 2116 70-71 -0.46586438442622935 2116 72-73 -0.4739852837278882 2116 74-75 -0.6448697108891999 2116 76 -0.06161419931979495 2117 1 0.2075486334104184 2117 2 0.05580490796488391 2117 3 -0.10304382789765398 2117 4 -0.045794122861433806 2117 5 1.0250458658514958 2117 6 -0.1341351723283566 2117 7 1.3673122354499547 2117 8 0.5689086914338652 2117 9 -1.1666209838707893 2117 10-11 0.13111137448780497 2117 12-13 0.19892277127653912 2117 14-15 0.5580545578146463 2117 16-17 -0.006010738324722098 2117 18-19 0.18654707033970297 2117 20-21 0.06939651510344547 2117 22-23 0.08140417777791242 2117 24-25 -0.10589156857283655 2117 26-27 0.3959069482776947 2117 28-29 0.15369265753146522 2117 30-31 0.40060533255370956 2117 32-33 0.37351918920005645 2117 34-35 0.10407673561584474 2117 36-37 -0.21503120384424435 2117 38-39 0.031884769989773076 2117 40-41 0.11097318019490388 2117 42-43 -0.08366374748347738 2117 44-45 -0.420024990599952 2117 46-47 0.34650401212138604 2117 48-49 -0.013499277090438966 2117 50-51 0.24482241303700647 2117 52-53 -0.24772731574120854 2117 54-55 0.1977851941435098 2117 56-57 0.18885756160031164 2117 58-59 0.3401380374016192 2117 60-61 -0.03435858338278308 2117 62-63 -0.05549246344036618 2117 64-65 -0.20304968173059734 2117 66-67 -0.15035058192403383 2117 68-69 0.04937517483689291 2117 70-71 0.2765413348710837 2117 72-73 -0.8521180075698105 2117 74-75 0.08140397555936474 2117 76 -0.1193121289979544 2118 1 0.2592028435473992 2118 2 0.15878172096389065 2118 3 -0.4887541051723545 2118 4 -0.3998741677559572 2118 5 0.6073482406697472 2118 6 0.09309233577051401 2118 7 0.28379821866552746 2118 8 -0.3791309994061187 2118 9 0.059254586876349435 2118 10-11 -0.6294809271217652 2118 12-13 0.1204466342462105 2118 14-15 0.5707576672705521 2118 16-17 0.35609165179848645 2118 18-19 0.2694871514559445 2118 20-21 -0.525162574762124 2118 22-23 0.2770907323783227 2118 24-25 -0.4057283157605518 2118 26-27 0.0307690761760675 2118 28-29 -0.19194415597770131 2118 30-31 0.20073542463849847 2118 32-33 -0.10575535611535614 2118 34-35 -0.41423818926235256 2118 36-37 -0.07640660878773886 2118 38-39 -0.2864582139655383 2118 40-41 0.5051777053587898 2118 42-43 0.17385480976979295 2118 44-45 -0.05958063685151416 2118 46-47 0.1969108687395007 2118 48-49 0.5015888539199409 2118 50-51 -0.027848045856444514 2118 52-53 -0.10268739532695292 2118 54-55 0.5593391568504522 2118 56-57 -0.15190717579223545 2118 58-59 -0.30930205646874853 2118 60-61 -0.32524193414675295 2118 62-63 -0.363096473337567 2118 64-65 -0.5175664278979824 2118 66-67 -0.4999411744807247 2118 68-69 -0.05843545190085564 2118 70-71 -0.4356873210991239 2118 72-73 -0.22518894601839534 2118 74-75 0.30957528897925357 2118 76 0.09505922013348922 2119 1 -0.37224521306217895 2119 2 0.19485494422304228 2119 3 -0.0832572330566741 2119 4 0.18242508360287601 2119 5 0.2753566500636566 2119 6 -0.20867158587854817 2119 7 0.6750145041747082 2119 8 0.6726329222429435 2119 9 0.5171819789387015 2119 10-11 0.38329981331511576 2119 12-13 0.5997308102281558 2119 14-15 0.7378302752082071 2119 16-17 0.9185852421994625 2119 18-19 0.29877901895671855 2119 20-21 0.08168544082602125 2119 22-23 0.22313472786596833 2119 24-25 0.5093316780862303 2119 26-27 0.49070908232928545 2119 28-29 0.03833991745066356 2119 30-31 0.3196719439698512 2119 32-33 0.08111009358211163 2119 34-35 0.01643558778615528 2119 36-37 0.3459731471347247 2119 38-39 0.2241048052119865 2119 40-41 0.12258874012445631 2119 42-43 0.3812694339109086 2119 44-45 0.1916371869614295 2119 46-47 -0.129850489069959 2119 48-49 -0.44590371094520265 2119 50-51 0.3309484878324085 2119 52-53 0.19649986751787907 2119 54-55 0.44281264674891574 2119 56-57 0.7827661910419366 2119 58-59 -0.008743139434596259 2119 60-61 -0.13286422929596853 2119 62-63 0.6185207966860133 2119 64-65 -0.14032306824256224 2119 66-67 -0.09277780662101875 2119 68-69 -0.22625157332430135 2119 70-71 0.21591251481514462 2119 72-73 -0.17675801585738782 2119 74-75 0.6390414975732455 2119 76 0.9198207830501133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 41.0 13 420.0 14 1242.0 15 1613.0 16 2124.0 17 2578.0 18 2744.0 19 2464.0 20 1832.0 21 1350.0 22 967.0 23 739.0 24 626.0 25 631.0 26 602.0 27 694.0 28 769.0 29 921.0 30 1113.0 31 1313.0 32 1710.0 33 2154.0 34 3066.0 35 4678.0 36 8561.0 37 30288.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.798846373652665 20.08080700681809 11.97219601015404 33.14815060937521 2 19.73790885288606 19.206283808030197 32.053003016972134 29.002804322111615 3 17.78684493826504 23.721109501468614 27.091612285854787 31.40043327441156 4 16.150768862721122 19.61962228040563 28.815406493799923 35.41420236307333 5 15.57395568905251 31.238287635730522 32.09420395794846 21.09355271726851 6 29.942451588894354 30.633564147206975 17.182121449741498 22.241862814157177 7 27.1155354128733 27.07433447189697 19.594370090774976 26.215760024454752 8 23.53769886099334 29.6859425047514 21.040390212782924 25.73596842147234 9 22.614000345556278 17.572865857710557 20.258901396844806 39.554232399888356 10-11 25.266144788080968 23.032655068380272 24.414215653699443 27.286984489839316 12-13 23.762974973751014 20.59050251857365 24.540476601852713 31.106045905822622 14-15 23.62541699339456 21.883015908879464 22.03120639013304 32.46036070759293 16-17 24.543134727076993 22.938956154224424 22.83794739570181 29.679961722996772 18-19 23.710477000571498 22.1421831182467 23.81281482170625 30.334525059475553 20-21 23.681902154410494 22.755545513749155 23.93243045679882 29.63012187504153 22-23 25.054159301444688 22.41597001634747 22.89576161932989 29.63410906287795 24-25 23.802182320809134 23.923127018513842 22.19002937228373 30.084661288393296 26-27 23.268563682035058 23.794207945136296 23.723767626692894 29.21346074613575 28-29 23.84869951223402 22.701053946651424 24.881381161866535 28.568865379248017 30-31 23.792878882524153 22.472455177363408 24.87407131749977 28.860594622612673 32-33 23.051261944950227 23.50247870177164 24.64347895429354 28.802780398984595 34-35 23.254608524607594 22.68111800746933 25.466833242514053 28.59744022540902 36-37 23.240653367180126 21.733496365013753 25.79776983293683 29.228080434869284 38-39 21.992663574380988 21.962759665607848 27.85715235044723 28.18742440956393 40-41 22.207307186241543 21.540782286253503 27.597320609773927 28.654589917731027 42-43 20.50477798009064 21.49094243829827 28.112332371978045 29.891947209633045 44-45 19.956539652583032 21.619861511675815 29.203492776544703 29.22010605919645 46-47 20.157892638322192 22.317619383049138 29.216783402666103 28.30770457596257 48-49 20.086787788572718 22.676466288326843 28.70110710915591 28.535638813944527 50-51 19.991759811804734 24.002206243936154 27.104238380670115 28.901795563589 52-53 18.747092675535946 23.7742720059542 27.676399835196236 29.802235483313623 54-55 19.087997235549768 23.325713374357065 27.28565542722718 30.30063396286599 56-57 19.61762868648742 23.858002950519 26.699538815273588 29.824829547719993 58-59 19.835594954878324 23.24596961762869 26.569290679283903 30.34914474820909 60-61 19.408965856381492 22.683776132693612 26.900891801012744 31.00636620991215 62-63 19.563137119389694 23.22470461583445 26.250980183676454 30.9611780810994 64-65 20.256907802926598 23.311758216929597 25.317978229954413 31.113355750189392 66-67 19.659494158769817 24.486649566061057 23.984263898672268 31.869592376496858 68-69 20.215706861950267 24.505256442631012 23.859332013131137 31.41970468228758 70-71 20.804481599128135 24.177642508738586 24.216185324490635 30.80169056764264 72-73 20.81777222524953 23.093791948538694 25.608378410706926 30.480057415504845 74-75 21.312848048271555 23.073191478050532 25.614359192461556 29.99960128121636 76 21.064313339801437 22.096994989433952 26.05893063622227 30.779761034542336 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 5.0 17 8.0 18 8.5 19 10.0 20 14.5 21 18.0 22 28.0 23 54.0 24 82.5 25 95.0 26 119.5 27 169.5 28 245.0 29 295.0 30 353.5 31 501.5 32 697.5 33 804.0 34 920.0 35 1216.0 36 1505.0 37 1614.0 38 1809.0 39 2305.5 40 2850.0 41 3212.0 42 3331.0 43 3350.0 44 3612.5 45 3850.5 46 3845.0 47 3837.0 48 3765.5 49 3548.0 50 3394.0 51 3164.5 52 2697.5 53 2316.5 54 2173.0 55 2088.0 56 1903.0 57 1765.0 58 1727.0 59 1752.5 60 1854.5 61 2076.0 62 2221.0 63 2281.0 64 2271.0 65 2181.0 66 2094.0 67 2027.0 68 1924.0 69 1789.5 70 1557.0 71 1356.0 72 1205.0 73 909.0 74 616.5 75 469.0 76 352.5 77 176.5 78 74.0 79 31.0 80 26.0 81 17.5 82 10.5 83 7.0 84 7.0 85 7.0 86 5.0 87 3.0 88 3.0 89 3.0 90 3.5 91 3.5 92 3.0 93 3.5 94 5.5 95 4.0 96 1.0 97 3.0 98 4.0 99 8.5 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 75241.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.15668319134515 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4915855731336 56.56357571005171 2 12.011804478977941 17.09440331734028 3 4.2623134537439995 9.098762642708099 4 1.8136311847438316 5.162079185550431 5 0.9096172883318703 3.2362674605600668 6 0.5024374754851604 2.1451070559934076 7 0.3436747044210762 1.7118326444358793 8 0.1849119333569921 1.052617588814609 9 0.14942378453090271 0.9569250807405536 >10 0.33060012327462224 2.9784293138049733 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013290626121396578 0.0 2 0.0 0.0 0.0 0.013290626121396578 0.0 3 0.0 0.0 0.0 0.013290626121396578 0.0 4 0.0 0.0 0.0 0.050504379261307 0.0 5 0.0 0.0 0.0 0.05714969232200529 0.0 6 0.0 0.0 0.0 0.05714969232200529 0.0 7 0.0 0.0 0.0 0.05714969232200529 0.0 8 0.0 0.0 0.0 0.05714969232200529 0.0 9 0.0 0.0 0.0 0.05847875493414495 0.0 10 0.0 0.0 0.0 0.05980781754628461 0.0 11 0.0 0.0 0.0 0.05980781754628461 0.0 12 0.0 0.0 0.0 0.06379500538270358 0.0 13 0.0 0.0 0.0 0.06778219321912256 0.0 14 0.0 0.0 0.0 0.07044031844340187 0.0 15 0.0 0.0 0.0 0.07309844366768119 0.0 16 0.0 0.0 0.0 0.07309844366768119 0.0 17 0.0 0.002658125224279316 0.0 0.07442750627982084 0.0 18 0.0 0.002658125224279316 0.0 0.07708563150410015 0.0 19 0.0 0.002658125224279316 0.0 0.08904719501335707 0.0 20 0.0 0.002658125224279316 0.0 0.09303438284977605 0.0 21 0.0 0.002658125224279316 0.0 0.0943634454619157 0.0 22 0.0 0.002658125224279316 0.0 0.09702157068619503 0.0 23 0.0 0.002658125224279316 0.0 0.09967969591047435 0.0 24 0.0 0.002658125224279316 0.0 0.10632500897117263 0.0 25 0.0 0.002658125224279316 0.0 0.11297032203187092 0.0 26 0.0 0.002658125224279316 0.0 0.12891907337754682 0.0 27 0.0 0.002658125224279316 0.0 0.23125689451230047 0.0 28 0.0 0.002658125224279316 0.0 0.36150503050198696 0.0 29 0.0 0.002658125224279316 0.0 0.4890950412673941 0.0 30 0.0 0.002658125224279316 0.0 0.6273175529299185 0.0 31 0.0 0.002658125224279316 0.0 0.7921213168352361 0.0 32 0.0 0.002658125224279316 0.0 0.9702157068619502 0.0 33 0.0 0.002658125224279316 0.0 1.1881819752528542 0.0 34 0.0 0.002658125224279316 0.0 1.4898791882085565 0.0 35 0.0 0.002658125224279316 0.0 1.9909357929852076 0.0 36 0.0 0.002658125224279316 0.0 2.68071928868569 0.0 37 0.0 0.002658125224279316 0.0 3.519357796945814 0.0 38 0.0 0.002658125224279316 0.0 4.238380670113369 0.0 39 0.0 0.002658125224279316 0.0 4.9374676040988295 0.0 40 0.0 0.002658125224279316 0.0 5.745537672279741 0.0 41 0.0 0.002658125224279316 0.0 6.60411211972196 0.0 42 0.0 0.002658125224279316 0.0 7.493255007243391 0.0 43 0.0 0.002658125224279316 0.0 8.359803830358448 0.0 44 0.0 0.002658125224279316 0.0 9.117369519278054 0.0 45 0.0 0.002658125224279316 0.0 9.963982403211014 0.0 46 0.0 0.002658125224279316 0.0 10.780026847064764 0.0 47 0.0 0.002658125224279316 0.0 11.562844725615024 0.0 48 0.0 0.002658125224279316 0.0 12.215414468175595 0.0 49 0.0 0.002658125224279316 0.0 12.930450153506731 0.0 50 0.0 0.002658125224279316 0.0 13.535173642030276 0.0 51 0.0 0.002658125224279316 0.0 14.109328690474609 0.0 52 0.0 0.002658125224279316 0.0 14.84031312715142 0.0 53 0.0 0.002658125224279316 0.0 15.605853191743863 0.0 54 0.0 0.002658125224279316 0.0 16.328863252747837 0.0 55 0.0 0.002658125224279316 0.0 16.973458619635572 0.0 56 0.0 0.002658125224279316 0.0 17.56223335681344 0.0 57 0.0 0.002658125224279316 0.0 18.169614970561263 0.0 58 0.0 0.002658125224279316 0.0 18.771680333860527 0.0 59 0.0 0.002658125224279316 0.0 19.26874975080076 0.0 60 0.0 0.002658125224279316 0.0 19.820310734838717 0.0 61 0.0 0.002658125224279316 0.0 20.410414534628725 0.0 62 0.0 0.002658125224279316 0.0 20.93539426642389 0.0 63 0.0 0.002658125224279316 0.0 21.510878377480363 0.0 64 0.0 0.002658125224279316 0.0 22.082375300700416 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATGA 15 0.0022168055 70.0 19 AACCGTG 20 0.006926924 52.500004 32 CAGAACT 20 0.006926924 52.500004 4 GAACTAA 20 0.006926924 52.500004 6 CGTCTTA 20 0.006926924 52.500004 6 AGTTATG 20 0.006926924 52.500004 18 GGGTTAG 20 0.006926924 52.500004 1 ACCGTGT 20 0.006926924 52.500004 33 GAACCGT 20 0.006926924 52.500004 31 TTCGAAA 20 0.006926924 52.500004 30 AATGAGC 30 5.8442284E-4 46.666664 32 CGGTGTA 35 0.0012483009 40.000004 34 CACAGAA 35 0.0012483009 40.000004 2 TGCTCTC 40 0.002405132 35.000004 40 ACAGAAC 40 0.002405132 35.000004 3 TCGGTGG 65 2.0781494E-5 32.307693 45 CTTAATA 55 2.9671166E-4 31.81818 2 ATCTCGG 45 0.0042831507 31.111109 42 CAGGAAT 45 0.0042831507 31.111109 13 AGATCTC 70 3.4481007E-5 30.000002 40 >>END_MODULE