FastQCFastQC Report
Wed 25 May 2016
SRR1780250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75241
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT840411.169442192421684No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG21962.9186214962586887No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC21362.838877739530309No Hit
CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG20142.676732100849271RNA PCR Primer, Index 32 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5320.707061309658298No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT2440.3242912773620765RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT1970.2618253345915126No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1200.15948751345675893No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1200.15948751345675893No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1090.1448678247232227No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT1050.13955157427466408No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT870.11562844725615024No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC780.10366688374689331No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATG150.002216805570.022
TCTTTAT850.057.647061
CTTTATA850.057.647062
TTTATAC900.054.4444433
CTATCTA200.00692692452.5000044
CACTGCC200.00692692452.5000043
CCCAAGA200.00692692452.5000048
GACTAGC200.00692692452.50000447
TTAATAC305.8442284E-446.6666643
TCTATAC404.5537672E-543.7500043
TATATAG400.00240513235.0000045
ACAAGGA400.00240513235.0000043
AAACTAA601.2015236E-535.070
TAATACA601.2015236E-535.04
CTTGAAA17650.034.3059557
TATGCCG18100.034.22651743
CTGCTTG17900.034.21787654
GTCTTCT17950.034.12256249
TGCTTGA17850.034.1176555
GCTTGAA17750.034.11267556