##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780250_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75241 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.724458739251205 34.0 34.0 34.0 34.0 34.0 2 33.74107202190295 34.0 34.0 34.0 34.0 34.0 3 33.76296168312489 34.0 34.0 34.0 34.0 34.0 4 33.760423173535706 34.0 34.0 34.0 34.0 34.0 5 33.772903071463695 34.0 34.0 34.0 34.0 34.0 6 37.52956499780705 38.0 38.0 38.0 37.0 38.0 7 37.52501960367353 38.0 38.0 38.0 37.0 38.0 8 37.451721800614024 38.0 38.0 38.0 37.0 38.0 9 37.47385069310615 38.0 38.0 38.0 37.0 38.0 10-11 37.43953429646071 38.0 38.0 38.0 37.0 38.0 12-13 37.41427546151699 38.0 38.0 38.0 37.0 38.0 14-15 37.332644435879374 38.0 38.0 38.0 37.0 38.0 16-17 37.36088037107428 38.0 38.0 38.0 36.5 38.0 18-19 37.29608192341941 38.0 38.0 38.0 36.0 38.0 20-21 37.319048125357185 38.0 38.0 38.0 36.5 38.0 22-23 37.31604444385375 38.0 38.0 38.0 36.0 38.0 24-25 37.315007775016284 38.0 38.0 38.0 36.0 38.0 26-27 37.22164112651347 38.0 38.0 38.0 36.0 38.0 28-29 37.14294068393562 38.0 38.0 38.0 36.0 38.0 30-31 37.133491048763304 38.0 38.0 38.0 36.0 38.0 32-33 37.01206124320517 38.0 38.0 38.0 36.0 38.0 34-35 37.05981446285934 38.0 38.0 38.0 36.0 38.0 36-37 37.08290028043221 38.0 38.0 38.0 36.0 38.0 38-39 36.085511888465064 38.0 37.0 38.0 34.0 38.0 40-41 36.575889475153176 38.0 37.0 38.0 34.5 38.0 42-43 37.039705745537674 38.0 37.5 38.0 36.0 38.0 44-45 37.137631078800126 38.0 38.0 38.0 36.0 38.0 46-47 37.09545992211693 38.0 38.0 38.0 36.0 38.0 48-49 37.11297032203187 38.0 38.0 38.0 36.0 38.0 50-51 36.935952472720984 38.0 38.0 38.0 35.0 38.0 52-53 36.92167169495355 38.0 38.0 38.0 35.0 38.0 54-55 36.919631583843916 38.0 37.5 38.0 35.5 38.0 56-57 36.947289376802544 38.0 38.0 38.0 36.0 38.0 58-59 36.7854494225223 38.0 37.5 38.0 35.0 38.0 60-61 36.779176246992996 38.0 37.0 38.0 35.0 38.0 62-63 36.78531651626108 38.0 37.0 38.0 35.0 38.0 64-65 36.63824909291476 38.0 37.5 38.0 34.5 38.0 66-67 36.174040749059685 38.0 37.0 38.0 33.5 38.0 68-69 35.99432490264616 38.0 37.0 38.0 33.0 38.0 70-71 34.90695897183716 38.0 36.0 38.0 26.5 38.0 72-73 32.95960314190401 38.0 32.5 38.0 16.0 38.0 74-75 32.417352241464094 38.0 31.0 38.0 11.0 38.0 76 31.785369678765566 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.25327108680859567 1101 2 -0.1378415625355558 1101 3 -0.040785804051211016 1101 4 0.0199130809137813 1101 5 -0.09081409712526778 1101 6 -0.06319233263689483 1101 7 0.027133279702411528 1101 8 -0.08291737986282044 1101 9 -0.39215278365237083 1101 10-11 -0.029357976462215163 1101 12-13 -0.0537932069824123 1101 14-15 0.04697749111233662 1101 16-17 0.12322130487336835 1101 18-19 -0.104542909284433 1101 20-21 0.18068661460333146 1101 22-23 -0.10735665652844517 1101 24-25 0.11273072989488497 1101 26-27 -0.4009598418497049 1101 28-29 -0.418226355184224 1101 30-31 -0.062149378524118504 1101 32-33 -0.2023790022619636 1101 34-35 0.2038424295544985 1101 36-37 0.0490829527057457 1101 38-39 0.4811777193404936 1101 40-41 -0.06205448333569308 1101 42-43 -0.11904571224891214 1101 44-45 -0.1393252604035169 1101 46-47 0.02320845973662955 1101 48-49 0.18526848772314963 1101 50-51 -0.0051243175024353604 1101 52-53 0.1614181183028407 1101 54-55 0.12471136224048962 1101 56-57 -0.6735308962970237 1101 58-59 -0.0017924938747739816 1101 60-61 -0.2619341754137139 1101 62-63 0.142322213748173 1101 64-65 0.10584917946844996 1101 66-67 -0.28416854288600746 1101 68-69 -0.10311148009214577 1101 70-71 0.08327817989195552 1101 72-73 -0.36717975793511926 1101 74-75 0.019392915960693813 1101 76 -0.6127412100794594 1102 1 -0.07519846111026851 1102 2 0.010319517538931677 1102 3 -0.017042787291437378 1102 4 -0.0070014907808158 1102 5 -0.01271819395952889 1102 6 0.03448573998880278 1102 7 -0.13607086368678978 1102 8 -0.28979587148293007 1102 9 -0.12661507415516127 1102 10-11 -0.2523270174305665 1102 12-13 0.08194352486116685 1102 14-15 0.01604755628924437 1102 16-17 0.0209022871824871 1102 18-19 0.0879471977919195 1102 20-21 -0.017608310908777014 1102 22-23 -0.07802704758989876 1102 24-25 0.12546059488557404 1102 26-27 0.050870349025530004 1102 28-29 -0.1352014482940902 1102 30-31 -0.029851892490604826 1102 32-33 0.2416386886132713 1102 34-35 0.23629994817648026 1102 36-37 0.20558027579700422 1102 38-39 0.017781536845156154 1102 40-41 0.032830293200618144 1102 42-43 0.0863488408237103 1102 44-45 0.15683686715700418 1102 46-47 0.22227445135674628 1102 48-49 -0.05088141916697708 1102 50-51 -0.409672152697965 1102 52-53 0.005837345490917301 1102 54-55 0.22604108756637942 1102 56-57 0.02298912232495809 1102 58-59 -0.17193623223604249 1102 60-61 0.00614950708162354 1102 62-63 -0.03244792126114504 1102 64-65 -0.017565043064145414 1102 66-67 0.09910950646222716 1102 68-69 -0.027270581581895215 1102 70-71 -0.9304555258808591 1102 72-73 -0.6997565735775737 1102 74-75 -0.822334854057921 1102 76 -0.9960629046790892 1103 1 -0.504218561612781 1103 2 -0.2414602144543636 1103 3 0.001801433814087261 1103 4 0.026734899503935594 1103 5 0.18629771893829172 1103 6 -0.0964030791066719 1103 7 -0.09992660070520998 1103 8 -0.19981471570403642 1103 9 -0.07338014953204919 1103 10-11 -0.11698886835183231 1103 12-13 -0.06935986709863329 1103 14-15 0.07412439883531619 1103 16-17 -0.05378102521282102 1103 18-19 -0.056355042576591075 1103 20-21 0.016808565138120457 1103 22-23 -0.036569761157736025 1103 24-25 -0.11615110946954132 1103 26-27 -0.01923277073996843 1103 28-29 -0.1461677380763362 1103 30-31 -0.3622953598272929 1103 32-33 0.02859430000355445 1103 34-35 -0.07016728381682924 1103 36-37 0.2564465865172707 1103 38-39 -0.3239793509270399 1103 40-41 -0.12067159122149462 1103 42-43 0.0024135393161728302 1103 44-45 0.026798655264201443 1103 46-47 0.10492991618087188 1103 48-49 0.02627519724843097 1103 50-51 -0.05787409993413917 1103 52-53 -0.008636063219299217 1103 54-55 -0.20613280271837908 1103 56-57 -0.09829437851256984 1103 58-59 -0.16331238382733204 1103 60-61 -0.48190273328687994 1103 62-63 -0.3905797261187658 1103 64-65 -0.2957397708698366 1103 66-67 -0.6953445345763072 1103 68-69 -0.5257133160877672 1103 70-71 -0.6224572009227387 1103 72-73 -0.6600266740800862 1103 74-75 -0.9107325718133694 1103 76 -0.524590961630512 1104 1 0.02443922004915322 1104 2 0.020283285654876693 1104 3 -0.037574188257622154 1104 4 -0.0907122395730866 1104 5 -0.039590174635861786 1104 6 0.06022547428831615 1104 7 -0.20377919702011837 1104 8 -0.0885579487776127 1104 9 -0.01142787608753082 1104 10-11 0.13241030141001886 1104 12-13 0.05182577123797927 1104 14-15 0.08496361909117667 1104 16-17 0.008183386216302324 1104 18-19 -0.08268233119358825 1104 20-21 0.03983432194146985 1104 22-23 0.09709372535695593 1104 24-25 -0.007880286756936528 1104 26-27 -0.020046015708771847 1104 28-29 0.10687282706822288 1104 30-31 -0.019737158191084347 1104 32-33 -0.13305937901958487 1104 34-35 -0.3253319962679697 1104 36-37 -0.01433971212570384 1104 38-39 -0.6952317481790047 1104 40-41 -0.40278262950469923 1104 42-43 -0.20871456497339125 1104 44-45 -0.23458070530676878 1104 46-47 -0.23443599550315497 1104 48-49 -0.18041040467422675 1104 50-51 -0.6321585657414488 1104 52-53 -0.3065871714172914 1104 54-55 -1.0476228617089873 1104 56-57 -0.6205797182547599 1104 58-59 -0.1897125003519804 1104 60-61 -0.30050055330484327 1104 62-63 -0.42514870628529877 1104 64-65 -0.3017966251897448 1104 66-67 -0.27766434619478275 1104 68-69 -0.4757533714369728 1104 70-71 -0.3984506949629889 1104 72-73 -0.8004510180220166 1104 74-75 -1.08414795792266 1104 76 -0.8032790882539658 1105 1 -0.3536638076449208 1105 2 -0.23864912932575066 1105 3 -0.296745757588468 1105 4 0.01501789865812242 1105 5 -0.22414558669880336 1105 6 -0.28126302238743506 1105 7 -0.5618650056009784 1105 8 -0.17871913880966162 1105 9 -0.1386819058383324 1105 10-11 0.006387916562836438 1105 12-13 -0.17873098008933397 1105 14-15 -0.06310879684605908 1105 16-17 0.08977089279305517 1105 18-19 -0.11690531870973331 1105 20-21 -0.08975146272395307 1105 22-23 -0.11268051293643566 1105 24-25 0.004663358911976445 1105 26-27 0.15588788202882853 1105 28-29 -0.18599559020828593 1105 30-31 -0.2671662489262516 1105 32-33 -0.33365009026461223 1105 34-35 -0.19606906997533713 1105 36-37 -0.25076770275085636 1105 38-39 -0.49077869417795483 1105 40-41 -0.1715323305617602 1105 42-43 -0.20928853541391135 1105 44-45 -0.12624378465817898 1105 46-47 -0.24485116745513125 1105 48-49 -0.1731999010151668 1105 50-51 -0.3636480190345992 1105 52-53 0.21071564582095448 1105 54-55 -0.38748510880326137 1105 56-57 -0.2813631879060665 1105 58-59 -0.17747975533835358 1105 60-61 -0.2058193460536799 1105 62-63 -0.5820817908981013 1105 64-65 -0.22659453976380917 1105 66-67 -0.5766072839668226 1105 68-69 -0.14884133240698105 1105 70-71 -0.27245222555082904 1105 72-73 -0.47976894981519536 1105 74-75 -0.5061477665991774 1105 76 -0.5871313865272718 1106 1 -0.02840358931539555 1106 2 -0.44705227733285824 1106 3 -0.12088894113759352 1106 4 0.06855940665507632 1106 5 -0.32714127088260625 1106 6 -0.010305926677865784 1106 7 -0.09095868419960595 1106 8 0.11805669261963203 1106 9 0.19117338738330147 1106 10-11 -0.01731099178953599 1106 12-13 -0.0029122443696039113 1106 14-15 -0.14829539242870027 1106 16-17 0.1505096589773558 1106 18-19 0.18454174907396492 1106 20-21 0.25458720191173256 1106 22-23 0.043767824204977046 1106 24-25 0.09304873591121066 1106 26-27 -0.1764934330257546 1106 28-29 -0.00226875598639964 1106 30-31 -5.249694136821859E-4 1106 32-33 0.22208740656198245 1106 34-35 0.38834321740724675 1106 36-37 -0.3139309421517211 1106 38-39 -0.10898128366766713 1106 40-41 -0.23503829654297448 1106 42-43 -0.16757744122757146 1106 44-45 -0.180623068740438 1106 46-47 -0.08289381787402306 1106 48-49 0.10777242698686962 1106 50-51 0.004630731917416142 1106 52-53 -0.03382611604753549 1106 54-55 0.057170895258686016 1106 56-57 0.2904570710429084 1106 58-59 -0.15058206556937392 1106 60-61 0.2564299557995824 1106 62-63 0.03289663002090748 1106 64-65 0.2164493800127829 1106 66-67 0.23809187825709444 1106 68-69 0.22725666200783934 1106 70-71 0.35992908950375835 1106 72-73 0.22860881103780883 1106 74-75 -0.2635608155963851 1106 76 -0.20744110980729147 1107 1 0.028968205556395787 1107 2 0.07281951753893168 1107 3 0.050665546041898324 1107 4 -0.012209824114151502 1107 5 0.05499013937379971 1107 6 -0.09572259334453292 1107 7 0.10351246964654592 1107 8 0.10603746185040563 1107 9 0.003593259178174435 1107 10-11 -0.01534785076389511 1107 12-13 -0.13420230847216175 1107 14-15 -0.17926494371075563 1107 16-17 -0.0363893794841772 1107 18-19 -0.4146569688747448 1107 20-21 0.1490583557578944 1107 22-23 0.10426461907677265 1107 24-25 0.021293928218902636 1107 26-27 0.0638911823588657 1107 28-29 0.2684443850392384 1107 30-31 -0.35276855915727623 1107 32-33 -0.11773631138672869 1107 34-35 -6.792184901911469E-4 1107 36-37 -0.054836390869667184 1107 38-39 0.45528153684514905 1107 40-41 0.42605945986728955 1107 42-43 -0.0074011591762896956 1107 44-45 0.06829520049033277 1107 46-47 -0.13189221530992512 1107 48-49 -0.3217147525003128 1107 50-51 0.12157784730203502 1107 52-53 0.2428165121575887 1107 54-55 0.11145775423304372 1107 56-57 -0.5343025443417133 1107 58-59 0.2551471010972932 1107 60-61 -0.29853799291837646 1107 62-63 -0.14442708792780934 1107 64-65 0.219414123602526 1107 66-67 -0.07797382687111565 1107 68-69 0.15241691841809768 1107 70-71 0.5799611407858052 1107 72-73 1.032014259755755 1107 74-75 1.4172484792754148 1107 76 2.0351870953209144 1108 1 -0.2597839533382498 1108 2 0.06871761771164842 1108 3 0.03088467384846183 1108 4 -0.04200257022814213 1108 5 -0.08406966200789157 1108 6 -0.10840609938943402 1108 7 0.1416439463304826 1108 8 0.1446277036466057 1108 9 -0.045494607833916234 1108 10-11 -0.06695892589342378 1108 12-13 -0.25751567203002423 1108 14-15 -0.3665895335207665 1108 16-17 0.055053301862969306 1108 18-19 0.15847558725651112 1108 20-21 0.16473732812404052 1108 22-23 0.032076579353102375 1108 24-25 -0.07371092929405165 1108 26-27 -0.049019007623869015 1108 28-29 0.027876056023693252 1108 30-31 0.04738256346794145 1108 32-33 0.08738566616076326 1108 34-35 -0.042372871339935614 1108 36-37 -0.1662758338748489 1108 38-39 -0.5629071505468985 1108 40-41 0.03501617402963575 1108 42-43 0.12949974755945703 1108 44-45 -0.08548556375837535 1108 46-47 0.09100016379198195 1108 48-49 0.14331115423543395 1108 50-51 -0.0991556371539275 1108 52-53 -0.027382645873501588 1108 54-55 -0.2954264210692017 1108 56-57 -0.27725915055933115 1108 58-59 -0.2916874196280901 1108 60-61 0.06194344058767598 1108 62-63 0.03360076181312621 1108 64-65 0.32117901997558107 1108 66-67 -0.18482561875366343 1108 68-69 -0.2835178829635865 1108 70-71 -0.08568005092404007 1108 72-73 0.47300897046388 1108 74-75 -0.21359402936016636 1108 76 0.7773071298632246 1109 1 0.03826307735126733 1109 2 -0.021411251691837663 1109 3 0.18167516142651152 1109 4 0.11471325280892586 1109 5 0.10875616501482455 1109 6 -0.020562336934276004 1109 7 0.20134900810808176 1109 8 -0.04604587148293149 1109 9 0.2270708232807337 1109 10-11 -0.03013150461004699 1109 12-13 0.12016467870731873 1109 14-15 0.005550761417453032 1109 16-17 -0.04692623845853916 1109 18-19 0.289669954202175 1109 20-21 0.18279233011686813 1109 22-23 0.14633192676907925 1109 24-25 0.22381796668043563 1109 26-27 0.36966041312809494 1109 28-29 0.133628679911034 1109 30-31 0.26357759468888275 1109 32-33 0.14772843220301013 1109 34-35 -0.13473370566967446 1109 36-37 0.09888957066879556 1109 38-39 0.23973666505028035 1109 40-41 0.10085913935446911 1109 42-43 0.273448199798068 1109 44-45 0.268094879977518 1109 46-47 0.4222343872541856 1109 48-49 0.18982370903815138 1109 50-51 0.12770765499433878 1109 52-53 0.2636097813883538 1109 54-55 0.02640166448944825 1109 56-57 0.41353399411982394 1109 58-59 0.4135204985331882 1109 60-61 0.3077120070816264 1109 62-63 -0.10043670331242538 1109 64-65 0.0036288671922690696 1109 66-67 0.29963033979555576 1109 68-69 0.25289768764886844 1109 70-71 0.6342880638627335 1109 72-73 0.051685734114734316 1109 74-75 -0.04048389251946105 1109 76 -0.5920564944226783 1110 1 -0.2470734611102685 1110 2 -0.24488881579439692 1110 3 0.014207212708562622 1110 4 0.0971651758858485 1110 5 0.03415680604047111 1110 6 -0.2675975933445329 1110 7 -0.07877919702011837 1110 8 0.14770412851706993 1110 9 0.04525992584483873 1110 10-11 0.02371464923610489 1110 12-13 0.10277685819449545 1110 14-15 -0.14020244371075563 1110 16-17 0.0990272871824871 1110 18-19 0.1712805311252552 1110 20-21 0.08655835575789439 1110 22-23 -0.005110380923227353 1110 24-25 0.24525226155223123 1110 26-27 0.2800370156921943 1110 28-29 -0.187284781627433 1110 30-31 -0.022039392490604826 1110 32-33 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2115 58-59 0.019193409387455063 2115 60-61 0.09303152348922339 2115 62-63 0.11391164264213671 2115 64-65 -6.450334047514161E-5 2115 66-67 0.133827230987265 2115 68-69 -0.10476140627965691 2115 70-71 0.09825777291015925 2115 72-73 -0.02180968238586445 2115 74-75 0.680188354059517 2115 76 0.7358563526611519 2116 1 0.06419944464889227 2116 2 0.1607340027047286 2116 3 -0.23824697577311582 2116 4 0.06501530677589784 2116 5 0.06985161406839069 2116 6 0.058454980826496694 2116 7 0.148751343992096 2116 8 -0.10049613326303586 2116 9 -0.260940990385528 2116 10-11 -0.010316764376462118 2116 12-13 0.17888396988734456 2116 14-15 0.28489027007633894 2116 16-17 -0.14973198419622236 2116 18-19 0.02976929639576298 2116 20-21 -0.12856489904140744 2116 22-23 0.23218237649386708 2116 24-25 0.012581559108959084 2116 26-27 0.13086325914769503 2116 28-29 0.1843066232591326 2116 30-31 0.326659887614106 2116 32-33 -0.008252236343096797 2116 34-35 0.21946148831609236 2116 36-37 0.14153959080572065 2116 38-39 0.012927697403618765 2116 40-41 -0.061001513082104 2116 42-43 0.045363893179732884 2116 44-45 0.15300560624950066 2116 46-47 0.18476627072847407 2116 48-49 0.3942538338871202 2116 50-51 0.2844132530612029 2116 52-53 0.12055702262878754 2116 54-55 0.10637213032379123 2116 56-57 0.10910386927085369 2116 58-59 0.26582282099258236 2116 60-61 0.09375040149698322 2116 62-63 0.29348194348580137 2116 64-65 -0.34414935807286184 2116 66-67 -0.41241154502120025 2116 68-69 -0.2604866592433339 2116 70-71 0.28660381094287146 2116 72-73 -0.5156924374519178 2116 74-75 -0.7332614465535627 2116 76 0.013044861464017288 2117 1 0.025689863762828224 2117 2 -0.10463395115819196 2117 3 0.09082523301312762 2117 4 0.07027748553051794 2117 5 0.1453824828594179 2117 6 0.0959546486182461 2117 7 0.2699940686313198 2117 8 -0.17701033848800307 2117 9 -0.0896544142566853 2117 10-11 -0.02450870101770164 2117 12-13 -0.1214987340220759 2117 14-15 0.0554140960523668 2117 16-17 -0.014855432783669187 2117 18-19 0.17454565379868114 2117 20-21 -0.058402515646506004 2117 22-23 0.08314046510045614 2117 24-25 -0.05576032305013001 2117 26-27 0.04633619081909757 2117 28-29 -0.6840857545546655 2117 30-31 -0.15659681634847544 2117 32-33 -0.189093121877427 2117 34-35 0.041278268819453956 2117 36-37 -0.18701437310316749 2117 38-39 0.21136233210742006 2117 40-41 0.01054285665239263 2117 42-43 -0.011922098262580505 2117 44-45 0.170333588815204 2117 46-47 0.058925783844053115 2117 48-49 -0.005724904784578655 2117 50-51 0.18245189552538932 2117 52-53 0.046155661903782175 2117 54-55 0.10057841836164272 2117 56-57 -0.4452321128696326 2117 58-59 0.31506937267089796 2117 60-61 -0.03526599630246352 2117 62-63 -0.19368152955217965 2117 64-65 -0.05874260228579686 2117 66-67 0.10419886771433795 2117 68-69 0.2098936527666595 2117 70-71 0.43608423723250667 2117 72-73 0.5221660152549106 2117 74-75 0.4793651247914852 2117 76 0.5435415792464653 2118 1 -0.051957255080118614 2118 2 -0.21130946068551992 2118 3 -0.229354345080381 2118 4 0.07196103015720468 2118 5 0.015443447581503733 2118 6 0.12470244853151513 2118 7 -0.02957484191124138 2118 8 0.18711995766280154 2118 9 0.22812738815639477 2118 10-11 0.16693072963811773 2118 12-13 0.05878083641895415 2118 14-15 0.15703897169963454 2118 16-17 0.12209837177210403 2118 18-19 0.04163490717215268 2118 20-21 0.1298738917712896 2118 22-23 0.1217216961286951 2118 24-25 0.13008004630935233 2118 26-27 0.1315209981042571 2118 28-29 0.057349849863364 2118 30-31 0.08745338389130808 2118 32-33 0.013518923119136161 2118 34-35 0.057973419700765305 2118 36-37 0.3041852799846083 2118 38-39 0.11571914153527274 2118 40-41 0.0049565494820313916 2118 42-43 0.05266479559758608 2118 44-45 0.02177352963606438 2118 46-47 0.10995504180900895 2118 48-49 -0.14206651129428138 2118 50-51 -0.05787409993414627 2118 52-53 0.1019167005998014 2118 54-55 0.17828930783438324 2118 56-57 0.2559769782713488 2118 58-59 0.13317002823296775 2118 60-61 -0.01959117549793632 2118 62-63 0.10439514825309004 2118 64-65 0.0032552040045388253 2118 66-67 0.10867556592620531 2118 68-69 -0.15134145679127897 2118 70-71 -0.4641657436363076 2118 72-73 -0.004247779607723601 2118 74-75 -0.4986722703058284 2118 76 -0.6652944792184528 2119 1 0.05490357970605686 2119 2 0.15870387128043006 2119 3 0.07479394740244061 2119 4 0.028606502416458568 2119 5 -0.02334744566021385 2119 6 0.129830127743908 2119 7 0.14145889821797653 2119 8 -0.16033158576864537 2119 9 0.1622879870693268 2119 10-11 0.039020771685081 2119 12-13 -0.045926373098019724 2119 14-15 -0.159441389288979 2119 16-17 0.11459914092398549 2119 18-19 0.09023248690755992 2119 20-21 -0.11571630410605138 2119 22-23 -0.019353578202142785 2119 24-25 -0.15137826565864998 2119 26-27 0.09620410752892639 2119 28-29 0.03773647687597048 2119 30-31 -0.11339781085795408 2119 32-33 0.014172702218708366 2119 34-35 -0.037350136857540406 2119 36-37 0.062351109130332816 2119 38-39 0.39384446201521683 2119 40-41 0.2327665176904148 2119 42-43 -0.07787309795180164 2119 44-45 -0.20067801379538253 2119 46-47 -0.011622232316717884 2119 48-49 0.13746892096907004 2119 50-51 0.12715820444489623 2119 52-53 0.04590962440248347 2119 54-55 -0.014126854610502448 2119 56-57 0.013847965862368028 2119 58-59 0.28496215211769993 2119 60-61 0.45039908191156286 2119 62-63 0.36035607533749925 2119 64-65 0.16871259299028196 2119 66-67 0.026671156122091588 2119 68-69 0.2797553537922468 2119 70-71 0.4300886918062119 2119 72-73 0.7613403651979382 2119 74-75 0.6927055370985329 2119 76 0.1674149864773753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 0.0 20 1.0 21 0.0 22 1.0 23 7.0 24 16.0 25 19.0 26 72.0 27 148.0 28 266.0 29 409.0 30 651.0 31 1052.0 32 1722.0 33 2994.0 34 5003.0 35 8630.0 36 11612.0 37 42636.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.19654177908321 14.84164218976356 13.080634228678514 39.881181802474714 2 17.221993328105686 17.544955542855625 40.07788306907138 25.155168059967302 3 16.85650110976728 16.411265134700496 36.20233649207214 30.529897263460082 4 13.707951781608433 27.35875387089486 31.48682234420064 27.446472003296073 5 14.470833720976595 28.649273667282465 41.958506665249004 14.921385946491938 6 26.55998724099892 39.834664611049824 16.16405948884252 17.441288659108732 7 25.116625244215257 23.100437261599392 22.11028561555535 29.672651878630003 8 21.306202735210857 39.78947648223708 14.339256522374768 24.565064260177298 9 20.297444212596854 15.806541646176953 14.95992876224399 48.936085378982206 10-11 24.517218006140272 28.228625350540266 23.30245477864462 23.951701864674845 12-13 23.484536356507753 17.372841934583537 27.081644316263738 32.060977392644965 14-15 20.391143126752702 16.36408341196954 19.61563509256921 43.629138368708546 16-17 29.02406932390585 25.446897303331962 16.847862202788374 28.68117116997382 18-19 32.273627410587316 22.661846599593307 17.233954891614943 27.830571098204437 20-21 24.18030063396287 19.31991866136814 17.263194269082017 39.23658643558698 22-23 21.415850400712376 26.959370555946894 17.459895535678687 34.16488350766205 24-25 29.21346074613575 25.927353437620447 18.130407623503142 26.72877819274066 26-27 30.359777249106205 29.244029186214966 17.812097127895697 22.58409643678314 28-29 22.682447070081473 27.66576733429912 19.678101035339775 29.973684560279633 30-31 22.001302481359897 22.440557674672053 25.622333568134394 29.935806275833656 32-33 19.88543480283356 25.708058106617404 21.685650110976727 32.72085697957231 34-35 20.684201432729495 19.398997886790447 27.68636780478728 32.23043287569277 36-37 29.834132986004967 23.258595712444013 21.82586621655746 25.081405084993552 38-39 21.90627450459191 22.77415239031911 28.35222817346925 26.96734493161973 40-41 20.813785037413112 17.13493972701054 29.856727050411347 32.194548185165 42-43 28.345582860408552 16.827261732300208 28.56155553488125 26.26559987240999 44-45 20.31538655786074 24.929891947209633 32.48561289722359 22.26910859770604 46-47 26.002445475206336 19.45216039127603 27.683045148256934 26.8623489852607 48-49 25.77384670591832 17.603434297789768 22.38872423279861 34.23399476349331 50-51 22.41929267287782 18.236068101168247 28.590794912348322 30.753844313605615 52-53 16.823939075769857 18.02142448930769 40.02139790805545 25.133238526867004 54-55 18.01810183277734 19.399662418096515 32.901609494823305 29.68062625430284 56-57 24.585664730665464 20.94469770470887 24.446113156390798 30.02352440823487 58-59 19.22555521590622 21.53280791058067 33.671801278558235 25.569835594954878 60-61 24.498611129570314 27.42321340758363 25.260828537632406 22.81734692521365 62-63 18.33043154663016 39.873207426801876 19.221568028069804 22.574792998498157 64-65 16.536861551547695 42.53066811977512 18.198854348028334 22.73361598064885 66-67 16.730904692920085 41.79436743264976 18.115787934769607 23.35893993966056 68-69 16.43585279302508 42.2801398173868 18.18755731582515 23.096450073762973 70-71 16.46775029571643 40.35765074892678 18.88398612458633 24.29061283077046 72-73 16.504299517550272 36.19635571031751 19.97315293523478 27.326191836897433 74-75 16.753498757326458 34.141624911949606 21.207852101912522 27.897024228811418 76 16.952193617841335 34.244627264390424 21.17728366183331 27.62589545593493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.5 16 3.0 17 3.0 18 3.5 19 8.5 20 14.0 21 15.0 22 15.5 23 30.0 24 46.0 25 48.0 26 65.0 27 115.5 28 177.5 29 206.0 30 248.0 31 303.0 32 457.0 33 598.0 34 683.5 35 876.5 36 1038.5 37 1093.0 38 1274.0 39 1802.5 40 4145.0 41 6985.0 42 7830.0 43 7267.5 44 5918.5 45 4414.0 46 3696.0 47 3454.0 48 2982.0 49 2608.5 50 2465.0 51 2252.5 52 1857.5 53 1524.0 54 1373.0 55 1390.5 56 1322.0 57 1328.0 58 1420.0 59 1388.5 60 1522.5 61 1767.0 62 1846.0 63 1913.5 64 1994.0 65 1974.5 66 1917.5 67 1893.0 68 1819.0 69 1679.5 70 1480.5 71 1347.0 72 1197.0 73 871.5 74 579.0 75 462.0 76 328.0 77 132.0 78 46.5 79 23.0 80 15.0 81 3.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 75241.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.60122805385362 #Duplication Level Percentage of deduplicated Percentage of total 1 62.79948789855514 27.381347935301232 2 21.16685972078278 18.45802155739557 3 7.6297018838017445 9.979931154556692 4 3.21282692190453 5.603327972780798 5 1.8472230689508016 4.027059714783164 6 1.0638297872340425 2.7830571098204437 7 0.7132841553374383 2.1770045586847595 8 0.43284764982015483 1.5098151273906515 9 0.24080960799853685 0.9449635172312967 >10 0.8352130707797355 4.8962666631225 >50 0.02438578308845943 0.7389588123496498 >100 0.01828933731634457 1.1895110378649938 >500 0.0030482228860574286 0.707061309658298 >1k 0.009144668658172286 8.43423133663827 >5k 0.0030482228860574286 11.169442192421684 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 8404 11.169442192421684 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 2196 2.9186214962586887 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 2136 2.838877739530309 No Hit CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG 2014 2.676732100849271 RNA PCR Primer, Index 32 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 532 0.707061309658298 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT 244 0.3242912773620765 RNA PCR Primer, Index 42 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT 197 0.2618253345915126 No Hit CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 120 0.15948751345675893 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 120 0.15948751345675893 No Hit CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 109 0.1448678247232227 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT 105 0.13955157427466408 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT 87 0.11562844725615024 No Hit CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 78 0.10366688374689331 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.014619688733536237 0.0 2 0.0 0.0 0.0 0.014619688733536237 0.0 3 0.0 0.0 0.0 0.014619688733536237 0.0 4 0.0 0.0 0.0 0.05449156709772597 0.0 5 0.0 0.0 0.0 0.061136880158424264 0.0 6 0.0 0.0 0.0 0.061136880158424264 0.0 7 0.0 0.0 0.0 0.061136880158424264 0.0 8 0.0 0.0 0.0 0.061136880158424264 0.0 9 0.0 0.0 0.0 0.06246594277056392 0.0 10 0.0 0.0 0.0 0.06379500538270358 0.0 11 0.0 0.0 0.0 0.06512406799484324 0.0 12 0.0 0.0 0.0 0.06911125583126221 0.0 13 0.0 0.0 0.0 0.07309844366768119 0.0 14 0.0 0.0 0.0 0.0757565688919605 0.0 15 0.0 0.0 0.0 0.07974375672837948 0.0 16 0.0 0.0 0.0 0.07974375672837948 0.0 17 0.0 0.0 0.0 0.08107281934051913 0.0 18 0.0 0.0 0.0 0.08373094456479845 0.0 19 0.0 0.0 0.0 0.09835063329833468 0.0 20 0.0 0.0 0.0 0.10366688374689331 0.0 21 0.0 0.0 0.0 0.10499594635903298 0.0 22 0.0 0.0 0.0 0.10632500897117263 0.0 23 0.0 0.0 0.0 0.1103121968075916 0.0 24 0.0 0.0 0.0 0.11562844725615024 0.0 25 0.0 0.001329062612139658 0.0 0.12360282292898818 0.0 26 0.0 0.001329062612139658 0.0 0.13955157427466408 0.0 27 0.0 0.001329062612139658 0.0 0.24587658324583672 0.0 28 0.0 0.001329062612139658 0.0 0.37479565662338354 0.0 29 0.0 0.001329062612139658 0.0 0.509030980449489 0.0 30 0.0 0.001329062612139658 0.0 0.6472534921120134 0.0 31 0.0 0.001329062612139658 0.0 0.8173735064658896 0.0 32 0.0 0.001329062612139658 0.0 1.002113209553302 0.0 33 0.0 0.001329062612139658 0.0 1.2147632274956472 0.0 34 0.0 0.001329062612139658 0.0 1.5377254422455842 0.0 35 0.0 0.001329062612139658 0.0 2.042769234858654 0.0 36 0.0 0.001329062612139658 0.0 2.7405271062319745 0.0 37 0.0 0.001329062612139658 0.0 3.5884690527770764 0.0 38 0.0 0.001329062612139658 0.0 4.3154663016174695 0.0 39 0.0 0.001329062612139658 0.0 5.015882298215069 0.0 40 0.0 0.001329062612139658 0.0 5.826610491620261 0.0 41 0.0 0.001329062612139658 0.0 6.6865140016746185 0.0 42 0.0 0.001329062612139658 0.0 7.600909078826704 0.0 43 0.0 0.001329062612139658 0.0 8.478090402838879 0.0 44 0.0 0.001329062612139658 0.0 9.236985154370622 0.0 45 0.0 0.001329062612139658 0.0 10.068978349570049 0.0 46 0.0 0.001329062612139658 0.0 10.892997169096637 0.0 47 0.0 0.001329062612139658 0.0 11.670498797198336 0.0 48 0.0 0.001329062612139658 0.0 12.333701040656026 0.0 49 0.0 0.001329062612139658 0.0 13.050065788599301 0.0 50 0.0 0.001329062612139658 0.0 13.642827713613588 0.0 51 0.0 0.001329062612139658 0.0 14.211666511609362 0.0 52 0.0 0.001329062612139658 0.0 14.941321885674034 0.0 53 0.0 0.001329062612139658 0.0 15.704203825042198 0.0 54 0.0 0.001329062612139658 0.0 16.424555760821892 0.0 55 0.0 0.001329062612139658 0.0 17.082441753831024 0.0 56 0.0 0.001329062612139658 0.0 17.67387461623317 0.0 57 0.0 0.001329062612139658 0.0 18.275939979532435 0.0 58 0.0 0.001329062612139658 0.0 18.88332159328026 0.0 59 0.0 0.001329062612139658 0.0 19.376403822384074 0.0 60 0.0 0.001329062612139658 0.0 19.930622931646308 0.0 61 0.0 0.001329062612139658 0.0 20.52072673143632 0.0 62 0.0 0.001329062612139658 0.0 21.062984277189297 0.0 63 0.0 0.001329062612139658 0.0 21.623848699512234 0.0 64 0.0 0.001329062612139658 0.0 22.199332810568706 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGAATG 15 0.0022168055 70.0 22 TCTTTAT 85 0.0 57.64706 1 CTTTATA 85 0.0 57.64706 2 TTTATAC 90 0.0 54.444443 3 CTATCTA 20 0.006926924 52.500004 4 CACTGCC 20 0.006926924 52.500004 3 CCCAAGA 20 0.006926924 52.500004 8 GACTAGC 20 0.006926924 52.500004 47 TTAATAC 30 5.8442284E-4 46.666664 3 TCTATAC 40 4.5537672E-5 43.750004 3 TATATAG 40 0.002405132 35.000004 5 ACAAGGA 40 0.002405132 35.000004 3 AAACTAA 60 1.2015236E-5 35.0 70 TAATACA 60 1.2015236E-5 35.0 4 CTTGAAA 1765 0.0 34.30595 57 TATGCCG 1810 0.0 34.226517 43 CTGCTTG 1790 0.0 34.217876 54 GTCTTCT 1795 0.0 34.122562 49 TGCTTGA 1785 0.0 34.11765 55 GCTTGAA 1775 0.0 34.112675 56 >>END_MODULE