FastQCFastQC Report
Wed 25 May 2016
SRR1780249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278635
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA301.3032502E-436.6442342
TCCGTTG250.00235054135.19741422
GAAACTC250.00235261335.19109326
CGAAACT353.216324E-431.4206225
AAGTCCC300.00570439929.36809217
ACTTTAG300.005749664729.32064833
TCAGATG300.005754711629.3153864
CTCTAAT300.005754711629.3153861
ACTATAC407.043617E-427.4831731
GTCAGAT407.043617E-427.4831733
ATAAGAG450.001390279424.46021814
AAATAAG450.001397575124.4382631
TGTATAA450.001397575124.4382611
CGGCATT500.002579286521.99443425
CTTAATA500.002584670721.986542
CACAACT500.002584670721.986544
GCGTGTA654.915544E-420.30984734
CGATAGC550.004481658319.99494210
TAATACA808.9991736E-519.2382224
TATAAGA600.007378305318.34186713